Plant Crispr Database

Plant Information

Name: CRT0137-4-2-1

Seed stock: 4949
Seed availability: No

Relationship:
Parent: CRT0137-4-2

Transformation: CRT0137
transformation CRT0137 to M82 sp+

Plant ID	Transformation	Construct	gRNA	Target Feature	Mutation	Phenotype
CRT0137-4-2-1 (T2 )	CRT0137	CTG0137-14	CGR0137-14	gene : Solyc03g118330 (LRRXII-RLK)	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: ACACCCATTCTGACTGCAATTATCGGTGTTATTTTATTTGCTGCAATAGCATGTTTTCTGCTTTCTATGTTTTTCATTAA AAGATCCCAAAAAACAAAGAGATGCAACATCCACTCTGGGCATCTCATCTTCCCCATGATTTCATATAAGGAGCTTCATG ATGCAACTAGTGGTTTTTCTTCAGATAGGTTGTTAAAATCAGGCAGTTTTGGCACTGCGACAAAGGAATTCTTCACTCAT CAAATGAAAATCCAGTTGCAGTCAAGGTACTCAACCTCCAAAGACACAGTGCATCTAAAAGCTTCATGGCTGAATGCCAC ACCTTGAGAGATACCCGGCATAGAAACCTGGTGAAAGTCATAACTGTATGATCCAGCATTGACTTCAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 827 TTTGAAGTCAATGCTGGATCATACAGTTATGACTTTCACCAGGTTTCTAT 876 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTTGAAGTCAATGCTGGATCATACAGTTATGACTTTCACCAGGTTTCTAT 50 Wildtype 877 GCCGGGTATCTCTCAAGGTGTGGCATTCAGCCATGAAGCTTTTAGATGCA 926 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GCCGGGTATCTCTCAAGGTGTGGCATTCAGCCATGAAGCTTTTAGATGCA 100 Wildtype 927 CTGTGTCTTTGGAGGTTGAGTACCTTGACTGCAACTGGATTTTCATTTGA 976 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CTGTGTCTTTGGAGGTTGAGTACCTTGACTGCAACTGGATTTTCATTTGA 150 Wildtype 977 TGAGTGAAGAATTCCTTTGTCGCAGTGCCAAAACTGCCTGATTTTAACAA 1026 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TGAGTGAAGAATTCCTTTGTCGCAGTGCCAAAACTGCCTGATTTTAACAA 200 Wildtype 1027 CCTATCTGAAGAAAAACCACTAGTTGCATCATGAAGCTCCTTATATGAAA 1076 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 CCTATCTGAAGAAAAACCACTAGTTGCATCATGAAGCTCCTTATATGAAA 250 Wildtype 1077 TCATGGGGAAGATGAGATGCCCAGATGTGGATGTTGCATCTCTTTGTTTT 1126 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TCATGGGGAAGATGAGATGCCCAGA-GTGGATGTTGCATCTCTTTGTTTT 299 gRNA AGATGTGGATGTTGCATCTC Wildtype 1127 TTGGGATCTTTTAATGAAAAACATAGAAAGCAGAAAACATGCTATTGCAG 1176 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 300 TTGGGATCTTTTAATGAAAAACATAGAAAGCAGAAAACATGCTATTGCAG 349 Wildtype 1177 CAAATAAAATAACACCGATAATTGCAGTCAGAATGGGTGT 1216 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 350 CAAATAAAATAACACCGATAATTGCAGTCAGAATGGGTGT 389

Plant ID

Transformation

Construct

gRNA

Target Feature

Mutation

Phenotype

CRT0137-4-2-1 (T2 )

CRT0137

CTG0137-14

CGR0137-14

gene : Solyc03g118330 (LRRXII-RLK)

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

ACACCCATTCTGACTGCAATTATCGGTGTTATTTTATTTGCTGCAATAGCATGTTTTCTGCTTTCTATGTTTTTCATTAA
AAGATCCCAAAAAACAAAGAGATGCAACATCCACTCTGGGCATCTCATCTTCCCCATGATTTCATATAAGGAGCTTCATG
ATGCAACTAGTGGTTTTTCTTCAGATAGGTTGTTAAAATCAGGCAGTTTTGGCACTGCGACAAAGGAATTCTTCACTCAT
CAAATGAAAATCCAGTTGCAGTCAAGGTACTCAACCTCCAAAGACACAGTGCATCTAAAAGCTTCATGGCTGAATGCCAC
ACCTTGAGAGATACCCGGCATAGAAACCTGGTGAAAGTCATAACTGTATGATCCAGCATTGACTTCAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         827 TTTGAAGTCAATGCTGGATCATACAGTTATGACTTTCACCAGGTTTCTAT    876
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TTTGAAGTCAATGCTGGATCATACAGTTATGACTTTCACCAGGTTTCTAT     50

Wildtype         877 GCCGGGTATCTCTCAAGGTGTGGCATTCAGCCATGAAGCTTTTAGATGCA    926
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GCCGGGTATCTCTCAAGGTGTGGCATTCAGCCATGAAGCTTTTAGATGCA    100

Wildtype         927 CTGTGTCTTTGGAGGTTGAGTACCTTGACTGCAACTGGATTTTCATTTGA    976
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 CTGTGTCTTTGGAGGTTGAGTACCTTGACTGCAACTGGATTTTCATTTGA    150

Wildtype         977 TGAGTGAAGAATTCCTTTGTCGCAGTGCCAAAACTGCCTGATTTTAACAA   1026
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 TGAGTGAAGAATTCCTTTGTCGCAGTGCCAAAACTGCCTGATTTTAACAA    200

Wildtype        1027 CCTATCTGAAGAAAAACCACTAGTTGCATCATGAAGCTCCTTATATGAAA   1076
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 CCTATCTGAAGAAAAACCACTAGTTGCATCATGAAGCTCCTTATATGAAA    250

Wildtype        1077 TCATGGGGAAGATGAGATGCCCAGATGTGGATGTTGCATCTCTTTGTTTT   1126
                     ||||||||||||||||||||||||| ||||||||||||||||||||||||
MutationRev      251 TCATGGGGAAGATGAGATGCCCAGA-GTGGATGTTGCATCTCTTTGTTTT    299
gRNA                                       AGATGTGGATGTTGCATCTC

Wildtype        1127 TTGGGATCTTTTAATGAAAAACATAGAAAGCAGAAAACATGCTATTGCAG   1176
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      300 TTGGGATCTTTTAATGAAAAACATAGAAAGCAGAAAACATGCTATTGCAG    349

Wildtype        1177 CAAATAAAATAACACCGATAATTGCAGTCAGAATGGGTGT   1216
                     ||||||||||||||||||||||||||||||||||||||||
MutationRev      350 CAAATAAAATAACACCGATAATTGCAGTCAGAATGGGTGT    389