Feature Information
Feature ID: Ppr-t_11345
Feature Type: gene
Name: SP5G
Description: SP5G
Links to other Database:
Feature Type: gene
Name: SP5G
Description: SP5G
Links to other Database:
Name: Ppr-t_11345
(Exon Intron)
Length: 1187
ATGCCAAAAGATCCTCTAATAGTTTCTGGAGTTGTTGGAGATGTTGTGGATCCATTCATAAGGTGTGTGGAGTTTAGTGTTGTTTACAACAACAATAGGGTTGTCTACAATGGTTGTGCCTTGAGGCCTTCACAAGTTGTCAACCAACCTAGGGTTGACATTGGTGGAGATGATCTTCGCACTTTTTACACTCTGGTAAA
CTTGTCGTCCTATTTTATACGATATATTTTTCTTTTTTTAATGCGTTCTCTAACGTCATTAGCTTCGTTTTTTTTTTTGAAATCATTTTGAAGTTGCTTG
GAATAGTTTAAGCAATTCACGCAATCGTATACCTTTTATTCATTGATTATGAGTAGGAGAACAAAAGCTAACATATATGTAAAGGTTTATAATTCTGCGA
ATTGTGCTGGTTCTAATTTTGATAAATTACATTTTAACTAAATATTCTTATTATATTTACATTTTATCCTATTACTTTTTAAAAATTACGAATATATACT
ATTTTTTTTGGTGAGACACAAACGGAGGGTTGCTTGATACATAACTTTTAGGCGCGACACATAACTCATATATATCGCGACTACACGATAAATCACGTGC
ATAGACATATATTTGAACGATTTATTAACATGTACTTATATGTATCTGGAGTGTTACTTGAGTTCTGTATCGTACCCAAGTTATGTATTGCGACCCTCCG
TATGTAACTAGAGGGTTTCTTGAGTTATTTATCGGGCCGAAGTTATATGTACGTATAGCGACCCTCCATATTGTATCACGCAATTAGTGTGATATGAGGA
GTAATTAACTCCTTTAGTACTGTATTTTATGTAATTTATCCTTCTAATTTCGATTCAGCAACATCATATTATACATTTGGATAGACAATATATGGTATCT
AGGTTATTTTGCTCTTTATTTTCTTAATTTTGGATTTCAAGATATTTGAAATATTCTTCTCTTTAATTTGGAAGATTATGGTGGATCCTGATGCTCCAAA
CCCTAGCAACCCAAATCTGAGGGAGTATCTACACTGGTAACTCTAACTTTAATATATATATATACTATGCATAGATAATAACAACATATATATATATATA
TATATATATATATATATATATATATATATATATATATATATATATCTATATATATCACAGAGAGTGTGAGAGAGATATATTATAAAC
The mutation and phenotype for selected feature: Ppr-t_11345
Project Name:Rapid improvement of domestication traits in an orphan crop by genome editing
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics res...... Lab: Lippman Lab, Cold Spring Harbor Laboratory; Van Eck lab, Boyce Thompson Institute; Contact: lippman@cshl.edu; jv27@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-16 (T0 ) | -40 bp / +1 bp | Showed more compact growth, and also more floral. Unaffected for primary shoot flowering. Sympodial shoots terminated more rapidly. |
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-16 (T0 ) | -40 bp / +1 bp | |
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-19 (T0 ) |
-40 bp
Is tDNA present:
Mutation sequence: ATGCCAAAAGATCCTCTAATAGTTTCTGGAGTTGTTGGAGATGTTGTGGATCCATTCATAAGGTGTGTGGAGTTTAGTGT TGTTTACAACAACAATAGGGTTGTCTACCTAGGGTTGACATTGGTGGAGATGATCTTCGCACTTTTTACACTCTGGTAAA CTTGTCGTCCTATTTTATACGATATATTTTTCTTTTTTTAATGCGTTCTCTAACGTCATTAGCTTCGTTTTTTTTTTTGA AATCATTTTGAAGTTGCTTGGAATAGTTTAAGCAATTCACGCAATCGTATACCTTTTATTCATTGATTATGAGTAGGAGA ACAAAAGCTAACATATATGTAAAGGTTTATAATTCTGCGAATTGTGCTGGTTCTAATTTTGATAAATTACATTTTAACTA AATATTCTTATTATATTTACATTTTATCCTATTACTTTTTAAAAATTACGAATATATACTATTTTTTTTGGTGAGACACA AACGGAGGGTTGCTTGATACATAACTTTTAGGCGCGACACATAACTCATATATATCGCGACTACACGATAAATCACGTGC ATAGACATATATTTGAACGATTTATTAACATGTACTTATATGTATCTGGAGTGTTACTTGAGTTCTGTATCGTACCCAAG TTATGTATTGCGACCCTCCGTATGTAACTAGAGGGTTTCTTGAGTTATTTATCGGGCCGAAGTTATATGTACGTATAGCG ACCCTCCATATTGTATCACGCAATTAGTGTGATATGAGGAGTAATTAACTCCTTTAGTACTGTATTTTATGTAATTTATC CTTCTAATTTCGATTCAGCAACATCATATTATACATTTGGATAGACAATATATGGTATCTAGGTTATTTTGCTCTTTATT TTCTTAATTTTGGATTTCAAGATATTTGAAATATTCTTCTCTTTAATTTGGAAGATTATGGTGGATCCTGATGCTCCAAA CCCTAGCAACCCAAATCTGAGGGAGTATCTACACTGGTAACTCTAACTTTAATATATATATATACTATGCATAGATAATA ACAACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATCTATATATATCACAG AGAGTGTGAGAGAGATATATTATAAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Showed more compact growth, and also more floral. Unaffected for primary shoot flowering. Sympodial shoots terminated more rapidly. |
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-19 (T0 ) |
-40 bp
Is tDNA present:
Mutation sequence: ATGCCAAAAGATCCTCTAATAGTTTCTGGAGTTGTTGGAGATGTTGTGGATCCATTCATAAGGTGTGTGGAGTTTAGTGT TGTTTACAACAACAATAGGGTTGTCTACCTAGGGTTGACATTGGTGGAGATGATCTTCGCACTTTTTACACTCTGGTAAA CTTGTCGTCCTATTTTATACGATATATTTTTCTTTTTTTAATGCGTTCTCTAACGTCATTAGCTTCGTTTTTTTTTTTGA AATCATTTTGAAGTTGCTTGGAATAGTTTAAGCAATTCACGCAATCGTATACCTTTTATTCATTGATTATGAGTAGGAGA ACAAAAGCTAACATATATGTAAAGGTTTATAATTCTGCGAATTGTGCTGGTTCTAATTTTGATAAATTACATTTTAACTA AATATTCTTATTATATTTACATTTTATCCTATTACTTTTTAAAAATTACGAATATATACTATTTTTTTTGGTGAGACACA AACGGAGGGTTGCTTGATACATAACTTTTAGGCGCGACACATAACTCATATATATCGCGACTACACGATAAATCACGTGC ATAGACATATATTTGAACGATTTATTAACATGTACTTATATGTATCTGGAGTGTTACTTGAGTTCTGTATCGTACCCAAG TTATGTATTGCGACCCTCCGTATGTAACTAGAGGGTTTCTTGAGTTATTTATCGGGCCGAAGTTATATGTACGTATAGCG ACCCTCCATATTGTATCACGCAATTAGTGTGATATGAGGAGTAATTAACTCCTTTAGTACTGTATTTTATGTAATTTATC CTTCTAATTTCGATTCAGCAACATCATATTATACATTTGGATAGACAATATATGGTATCTAGGTTATTTTGCTCTTTATT TTCTTAATTTTGGATTTCAAGATATTTGAAATATTCTTCTCTTTAATTTGGAAGATTATGGTGGATCCTGATGCTCCAAA CCCTAGCAACCCAAATCTGAGGGAGTATCTACACTGGTAACTCTAACTTTAATATATATATATACTATGCATAGATAATA ACAACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATCTATATATATCACAG AGAGTGTGAGAGAGATATATTATAAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-29 (T0 ) | +1 bp and +1 bp / -3 bp and +1 bp | Showed more compact growth, and also more floral. Unaffected for primary shoot flowering. Sympodial shoots terminated more rapidly. |
| SP5G-sgRNA1 | Physalis pruinosa | SP5G-sgRNA1 | SP5G-sgRNA1 | Ppr-sp5gCR-29 (T0 ) | +1 bp and +1 bp / -3 bp and +1 bp |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
