Submit Data to Plant Genome Editing Database

Prepare your data Submit your data

Prepare your data

You can prepare your dataset using this template. 1) download the blank template file (xlsx format). 2) prepare tables according to the template. If you have any question related to dataset preparation, please contact us (feibioinfolab@btiscience.org).

Meta data

1. *Project Name: Project name, e.g. Using CRISPR to understand and improve the plant immune system (NSF IOS-1546625)
2. *Project Description: Description of the project, maximum 250 words
3. *Lab: Laboratory name, University or Institute name, e.g. Martin Lab, BTI at Cornell University
4. *Contact: Name and email address, e.g. Gregory Martin: gbm7@cornell.edu
5. *Plant Species: Name of the plant species, e.g. Tomato
6. *Reference Genome: URL of the reference genome used for the project. If multiple version available, please indicates which version is used, e.g. SL2.50 (ftp://ftp.solgenomics.net/tomato_genome/assembly/build_2.50/)
7. Plasmid: Name of plasmid, e.g. p201N:Cas9
8. Plasmid Structure: URL of plasmid structure, e.g. https://www.addgene.org/59175
9. Gene Links: External database to which the gene is linked, supporting multiple databases, one URL per row, e.g. http://itak.feilab.net

Transformation/Construct/gRNA

1. *Transformation Name: Name designated to each transformation experiment, e.g. NNN0001, N can be any letter, but different from Construct Name and gRNA Name.
2. *Genetic Background: Plant varieties used for transformation
3. *Construct Name: Name designated to each construct, e.g. NNN0001, N can be any letter, but different from Transformation Name and gRNA Name.
4. *gRNA Name: Name designated to each gRNA, e.g. NNN0001, N can be any letter, but different from Transformation Name and Construct Name.
5. *gRNA Sequence: 19-20 nt gRNA sequence
6. *Target Feature ID: Target feature identifier
7. Feature Name: Name or short description of the feature
8. Feature Type: Feature type such as: gene, promoter. Please check the Sequence Ontology to get appropriate terminology.
9. Feature Location: The location of a feature relative to genome. Format: chr:start-end. E.g.: chr01:10001-10500.
10. Feature Strand: The orientation/directionality of the feature. Should be + or -.
11. *Primers: Primers used for genotyping the mutated site of a target gene
12. Publication: Papers related to plant mutants deposited in the database

Plant mutation

1. *Plant Generation: Generation of the plant, e.g. T0, T1, T2......
2. *Plant Name: Name designated to each plant
3. *Progenitor: Parent of the plant
4. *Transformation Name: Name designated to each transformation experiment
5. *Target Feature: The feature that gRNA targets
6. *Mutation: Mutation type of the plant. Mutation type can be very specific, e.g. -1 bp (48%); -3 bp (48%) , or in general, e.g. heterozygous/homozygous/biallelic/chimeric mutation
7. Mutated Sequence: Provide the mutated sequence only if the mutation is homozgyous
8. *gRNA Name: Name designated to each gRNA
9. Is T-DNA Present?: Yes or No
10. Seed Stock: Stock # used in your lab
11. Seed Availability: Yes or No
12. Plant phenotype (in PDF format): Plant morphology and any traits studied in your project; Information should be provided in pdf format. The pdf files should be sent along with this form. The database only accpets one pdf per transformation, please merge the phenotypic images into single pdf for each transformation.
13. Morphology: Any change in plant growth and development, such as leaf shape, plant structure, fruit shape and size, etc.
14. Other penotype tested in your lab: Other traits studied in your project

* required field

Submit your data

Please send your dataset to feibioinfolab@btiscience.org.