ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTAA
GAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTGGCTGCC
GTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCA
TCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAGACCCCGT
GACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAAT
CTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCAGAAAATT
GTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAGAACGAA
TTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGA
GCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGAGAACAAT
ATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCAT
CAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTAA
GAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTGGCTGCC
GTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCA
TCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAGACCCCGT
GACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAAT
CTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCAGAAAATT
GTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAGAACGAA
TTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGA
GCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGAGAACAAT
ATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCAT
CAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGAG    350
                     ||||||||||||||||||      ||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCT------AAGAGGCGCAGCATCCACAGGAGGAG    344

Wildtype         351 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         345 AAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTTG    394

Wildtype         401 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         395 GCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCT    444

Wildtype         451 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         445 GAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATTC    494

Wildtype         501 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         495 TTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTG    544

Wildtype         551 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         545 AAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATT    594

Wildtype         601 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         595 GATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTGT    644

Wildtype         651 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         645 TGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACG    694

Wildtype         701 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         695 AAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGATG    744

Wildtype         751 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         745 ACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGAG    794

Wildtype         801 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         795 AACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGA    844

Wildtype         851 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         845 AAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGTG    894

Wildtype         901 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         895 AAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAAT    944

Wildtype         951 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         945 GATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTG    994

Wildtype        1001 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1050
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         995 CTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAAG   1044

Wildtype        1051 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1045 GTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1092

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCC-AAGAAGAGGCGCAGCATCCACAGGAGGA    349
                     ||||||||||||||||||||| ||||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCTTCCAAAGAAGAGGCGCAGCATCCACAGGAGGA    350

Wildtype         350 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    400

Wildtype         400 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    450

Wildtype         450 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    500

Wildtype         500 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    550

Wildtype         550 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    600

Wildtype         600 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    650

Wildtype         650 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    700

Wildtype         700 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    750

Wildtype         750 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    800

Wildtype         800 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    850

Wildtype         850 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    900

Wildtype         900 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    950

Wildtype         950 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT   1000

Wildtype        1000 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1050

Wildtype        1050 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     |||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1099

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
ACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACA
TTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTTC
CAAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAA    200
gRNA                       CCAGACAAAATAAGACAACC

Wildtype         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAGA    250

Wildtype         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACT    300

Wildtype         301 GAACAACCAAGAAAATCTTCC-AAGAAGAGGCGCAGCATCCACAGGAGGA    349
                     ||||||||||||||||||||| ||||||||||||||||||||||||||||
Mutation         301 GAACAACCAAGAAAATCTTCCAAAGAAGAGGCGCAGCATCCACAGGAGGA    350

Wildtype         350 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    400

Wildtype         400 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    450

Wildtype         450 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    500

Wildtype         500 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    550

Wildtype         550 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    600

Wildtype         600 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    650

Wildtype         650 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    700

Wildtype         700 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    750

Wildtype         750 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    800

Wildtype         800 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    850

Wildtype         850 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    900

Wildtype         900 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    950

Wildtype         950 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT   1000

Wildtype        1000 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1050

Wildtype        1050 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     |||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1099

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAAC
ATTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTT
CCAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCA-GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    199
                     ||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    200
gRNA                       CCA-GACAAAATAAGACAACC

Wildtype         200 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    250

Wildtype         250 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    300

Wildtype         300 TGAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TGAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGA    350

Wildtype         350 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    400

Wildtype         400 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    450

Wildtype         450 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    500

Wildtype         500 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    550

Wildtype         550 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    600

Wildtype         600 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    650

Wildtype         650 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    700

Wildtype         700 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    750

Wildtype         750 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    800

Wildtype         800 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    850

Wildtype         850 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    900

Wildtype         900 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    950

Wildtype         950 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT   1000

Wildtype        1000 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1050

Wildtype        1050 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     |||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1099

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAAC
ATTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTT
CCAAGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT
GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCTC
TTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTAG
ACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGAT
TTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACCA
GAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA
GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGTT
TCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGGA
GAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATC
TAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCA-GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    199
                     ||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    200
gRNA                       CCA-GACAAAATAAGACAACC

Wildtype         200 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    250

Wildtype         250 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    300

Wildtype         300 TGAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGA    349
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TGAACAACCAAGAAAATCTTCCAAGAAGAGGCGCAGCATCCACAGGAGGA    350

Wildtype         350 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    399
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 GAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTTT    400

Wildtype         400 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    449
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 GGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCC    450

Wildtype         450 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    499
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTATT    500

Wildtype         500 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    549
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 CTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCT    550

Wildtype         550 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    599
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 GAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCAT    600

Wildtype         600 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    649
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCTG    650

Wildtype         650 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    699
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 TTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGAC    700

Wildtype         700 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    749
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 GAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGAT    750

Wildtype         750 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    799
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 GACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTGA    800

Wildtype         800 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    849
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 GAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGG    850

Wildtype         850 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    899
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 AAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGGT    900

Wildtype         900 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    949
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 GAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAA    950

Wildtype         950 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT    999
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 TGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTT   1000

Wildtype        1000 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1049
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 GCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCAA   1050

Wildtype        1050 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     |||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 GGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1099

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAAC
ATTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTT
CCAAGGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT
TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCT
CTTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTA
GACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGA
TTTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACC
AGAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG
AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGT
TTCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGG
AGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGAT
CTAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCA-GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    199
                     ||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    200
gRNA                       CCA-GACAAAATAAGACAACC

Wildtype         200 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    250

Wildtype         250 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    300

Wildtype         300 TGAACAACCAAGAAAATCTTCCAA-GAAGAGGCGCAGCATCCACAGGAGG    348
                     |||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation         301 TGAACAACCAAGAAAATCTTCCAAGGAAGAGGCGCAGCATCCACAGGAGG    350

Wildtype         349 AGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT    398
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 AGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT    400

Wildtype         399 TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCC    448
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCC    450

Wildtype         449 CTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTAT    498
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 CTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTAT    500

Wildtype         499 TCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACC    548
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACC    550

Wildtype         549 TGAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCA    598
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TGAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCA    600

Wildtype         599 TTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCT    648
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCT    650

Wildtype         649 GTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGA    698
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGA    700

Wildtype         699 CGAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGA    748
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CGAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGA    750

Wildtype         749 TGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG    798
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG    800

Wildtype         799 AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAG    848
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAG    850

Wildtype         849 GAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGG    898
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGG    900

Wildtype         899 TGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAA    948
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 TGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAA    950

Wildtype         949 ATGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCT    998
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 ATGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCT   1000

Wildtype         999 TGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCA   1048
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 TGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCA   1050

Wildtype        1049 AGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 AGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1100

ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTTTTACAAGCTCAAGGACATGGGCAAAAACAG
CAGCAGCAGAAGTAGAGGCCATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACTCTACCACCAG
GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAAAATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAAC
ATTCTCCCAGATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCACTGAACAACCAAGAAAATCTT
CCAAGGAAGAGGCGCAGCATCCACAGGAGGAGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT
TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCCCTGAAGAATACCCAAATACACCTGATTCCT
CTTCCTCATCGTCTTCTTATTCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACCTGAAAAATTA
GACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCATTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGA
TTTTCAATCTGTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGACGAAGAATTCGACCAAAACC
AGAAAATTGTGAAAGAAGTACAGAAAATGATGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG
AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAGGAAAAGTATTTCCTCAAGGAGGACAAGTGT
TTCAGAGAGCTTTGCAGTGGTGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAAATGATAGTGG
AGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCTTGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGAT
CTAATCATCAAGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ATGGGGAATTACAGGTTTAGATTATCAGATATGATGCCAAATGCTTGGTT     50

Wildtype          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TTACAAGCTCAAGGACATGGGCAAAAACAGCAGCAGCAGAAGTAGAGGCC    100

Wildtype         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 ATAGCCACACCACCTCATTTTCATCAACAACATCATACTGCTCATCAACT    150

Wildtype         151 CTACCACCA-GACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    199
                     ||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation         151 CTACCACCAGGACAAAATAAGACAACCCCACCAGCTCGGTTGCCAAAGAA    200
gRNA                       CCA-GACAAAATAAGACAACC

Wildtype         200 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    249
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 AATCATATTATATTTCAAGAAACCTCACTCCTGCTGAAACATTCTCCCAG    250

Wildtype         250 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    299
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATGAATCCTGTGTCTAACAGCCCAAAAGCCTCTGACTATTTCAATTTCAC    300

Wildtype         300 TGAACAACCAAGAAAATCTTCCAA-GAAGAGGCGCAGCATCCACAGGAGG    348
                     |||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation         301 TGAACAACCAAGAAAATCTTCCAAGGAAGAGGCGCAGCATCCACAGGAGG    350

Wildtype         349 AGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT    398
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 AGAAATTCTCACAAACTTGTCACTTCCGGATCCTCAGTTTCAGCTAGTTT    400

Wildtype         399 TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCC    448
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 TGGCTGCCGTGCTTCTCTTGAATCTGTCTGGACTAAAGCTGATTCCACCC    450

Wildtype         449 CTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTAT    498
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 CTGAAGAATACCCAAATACACCTGATTCCTCTTCCTCATCGTCTTCTTAT    500

Wildtype         499 TCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACC    548
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 TCTTCGTCTGATCAAGTTTCAGTTTTACCTCAATTCAAAGTCCATACACC    550

Wildtype         549 TGAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCA    598
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 TGAAAAATTAGACCCCGTGACTTCAATTTCACCGGTTTGTGGCTGCAGCA    600

Wildtype         599 TTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCT    648
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         601 TTGATGACATGAAGAAAGATTTGGTCAATATTGATCATGATTTTCAATCT    650

Wildtype         649 GTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGA    698
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         651 GTTGCGAACATTGATCTTCCCCCTATCATAACCAAGCCAAAAAAACTAGA    700

Wildtype         699 CGAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGA    748
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         701 CGAAGAATTCGACCAAAACCAGAAAATTGTGAAAGAAGTACAGAAAATGA    750

Wildtype         749 TGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG    798
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         751 TGACTCCAGTAAGAAGAGTTTCAGTGAGTTCATCAACTGGTGTGAAGCTG    800

Wildtype         799 AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAG    848
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         801 AGAACGAATTCACCAAGAATAACAAACAACAGAAAGATTCAAGGAAGCAG    850

Wildtype         849 GAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGG    898
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         851 GAAAAGTATTTCCTCAAGGAGGACAAGTGTTTCAGAGAGCTTTGCAGTGG    900

Wildtype         899 TGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAA    948
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         901 TGAAATCATCTAGAAATCCACAAAAAGATTTCAGGGAATCAATGGTGGAA    950

Wildtype         949 ATGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCT    998
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         951 ATGATAGTGGAGAACAATATAAGGGCATCAAAGGATTTGGAAGAACTTCT   1000

Wildtype         999 TGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCA   1048
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1001 TGCTTGCTATCTTTCTTTAAATTCAGATGAGTATCATGATCTAATCATCA   1050

Wildtype        1049 AGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1098
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation        1051 AGGTGTTCAAGCAAATCTGGTTCGACATCACTGAGATTCGACTAAAGTAA   1100