Feature Information
Feature ID: Ppr-t_53014
Feature Type: mRNA
Name: MPF2
Description: MPF2
Links to other Database:
Feature Type: mRNA
Name: MPF2
Description: MPF2
Links to other Database:
Name: Ppr-t_53014
(Exon Intron)
Length: 768
ATGGCAAGAGAGAAGATCAAGATAAAGAAGATCGACAATATAACAGCAAGACAAGTTACATTTTCAAAGAGGAGAAGAGGACTTTTCAAGAAAGCTGAAGAGCTATCTGTTCTCTGTGATGCTGATGTTGCTCTTATCATTTTCTCTTCTACTGGAAAACTCTTTGATTTTTCTAGCTCCAGCATGAAGGATATCCTTGG
AAAATATAAGTTGCAGTCTGCTAACCTTGACAAAGTTGACCAACCTTCTCTTGACTTACAGCTAGAGAATAGCCTCAACGTGAGATTACGCAAGCAGGTA
GCTGATAAAACTCGTGAGCTCAGGCAGATGAAAGGTGAGGAACTTGAAGGATTGAGTTTAGAAGAATTACAACAAATTGAGAAAAGACTTGAAGCTGGAT
TCAACCGTGTGCTTGAGATTAAGGGTACAAGAATTATGGATGAAATTGCCAACCTTCAAAGAAAGGGTGCTGAGCTGATGGAAGAAAACAAAAAATTGAA
ACATAAAATGGAAATGATGAAGTTAGGAAAGTTTCCCTTACTAACAGACATGGACTGCATGGTTATGGAAGAAGGCCAATCATCTGACTCTATAATTACC
ACCAATAATGTTTGCTCTAGTAATAGTGGCCCTCCTCCTGAAGATGATTCCTCAAATGCTTCTTTGAAGTTAGGTTGTAACAATGGTCTAGCACCTGTGG
ATGATGATTGCTCAATTACATCTTTGAAGTTAGGGTTTACTTCTAACCTTCTGATTGTTTTTGAGTAA
The mutation and phenotype for selected feature: Ppr-t_53014
Project Name:Rapid improvement of domestication traits in an orphan crop by genome editing
Genome editing holds great promise for increasing crop productivity, and there is particular interest in advancing breeding in orphan crops, which are often burdened by undesirable characteristics res...... Lab: Lippman Lab, Cold Spring Harbor Laboratory; Van Eck lab, Boyce Thompson Institute; Contact: lippman@cshl.edu; jv27@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| MPF2-sgRNA1 | Physalis pruinosa | MPF2-sgRNA1 | MPF2-sgRNA1 | Ppr-mpf2CR-a1 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGCAAGAGAGAAGATCAAGATAAAGAAGATCGACAATATAACAGCAAGACAAGTTACATTTTCAAAGAGGAGAAGAGG ACTTTTCAAGAAAGCTGAAGAGCTATCTGTTCTCTGTGATGCTGATGTTGCTCTTATCATTTTCTCTTCTACTGGAAAAC TCTTTGATTTTTCTAGCTCCAGCATGAAGGATATCCTTGGAAAATATAAGTTGCAGTCTGCTAACCTTGACAAAGTTGAC CAACCTTCTCTTGACTTACAGCTAGAGAATAGCCTCAACGTGAGATTACGCAAAGCAGGTAGCTGATAAAACTCGTGAGC TCAGGCAGATGAAAGGTGAGGAACTTGAAGGATTGAGTTTAGAAGAATTACAACAAATTGAGAAAAGACTTGAAGCTGGA TTCAACCGTGTGCTTGAGATTAAGGGTACAAGAATTATGGATGAAATTGCCAACCTTCAAAGAAAGGGTGCTGAGCTGAT GGAAGAAAACAAAAAATTGAAACATAAAATGGAAATGATGAAGTTAGGAAAGTTTCCCTTACTAACAGACATGGACTGCA TGGTTATGGAAGAAGGCCAATCATCTGACTCTATAATTACCACCAATAATGTTTGCTCTAGTAATAGTGGCCCTCCTCCT GAAGATGATTCCTCAAATGCTTCTTTGAAGTTAGGTTGTAACAATGGTCTAGCACCTGTGGATGATGATTGCTCAATTAC ATCTTTGAAGTTAGGGTTTACTTCTAACCTTCTGATTGTTTTTGAGTAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| MPF2-sgRNA1 | Physalis pruinosa | MPF2-sgRNA1 | MPF2-sgRNA1 | Ppr-mpf2CR-a1 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGCAAGAGAGAAGATCAAGATAAAGAAGATCGACAATATAACAGCAAGACAAGTTACATTTTCAAAGAGGAGAAGAGG ACTTTTCAAGAAAGCTGAAGAGCTATCTGTTCTCTGTGATGCTGATGTTGCTCTTATCATTTTCTCTTCTACTGGAAAAC TCTTTGATTTTTCTAGCTCCAGCATGAAGGATATCCTTGGAAAATATAAGTTGCAGTCTGCTAACCTTGACAAAGTTGAC CAACCTTCTCTTGACTTACAGCTAGAGAATAGCCTCAACGTGAGATTACGCAAAGCAGGTAGCTGATAAAACTCGTGAGC TCAGGCAGATGAAAGGTGAGGAACTTGAAGGATTGAGTTTAGAAGAATTACAACAAATTGAGAAAAGACTTGAAGCTGGA TTCAACCGTGTGCTTGAGATTAAGGGTACAAGAATTATGGATGAAATTGCCAACCTTCAAAGAAAGGGTGCTGAGCTGAT GGAAGAAAACAAAAAATTGAAACATAAAATGGAAATGATGAAGTTAGGAAAGTTTCCCTTACTAACAGACATGGACTGCA TGGTTATGGAAGAAGGCCAATCATCTGACTCTATAATTACCACCAATAATGTTTGCTCTAGTAATAGTGGCCCTCCTCCT GAAGATGATTCCTCAAATGCTTCTTTGAAGTTAGGTTGTAACAATGGTCTAGCACCTGTGGATGATGATTGCTCAATTAC ATCTTTGAAGTTAGGGTTTACTTCTAACCTTCTGATTGTTTTTGAGTAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| MPF2-sgRNA1 | Physalis pruinosa | MPF2-sgRNA1 | MPF2-sgRNA1 | Ppr-mpf2CR-a2 (T0 ) |
+45 bp and -54 bp
Is tDNA present:
Mutation sequence: ATGGCAAGAGAGAAGATCAAGATAAAGAAGATCGACAATATAACAGCAAGACAAGTTACATTTTCAAAGAGGAGAAGAGG ACTTTTCAAGAAAGCTGAAGAGCTATCTGTTCTCTGTGATGCTGATGTTGCTCTTATCATTTTCTCTTCTACTGGAAAAC TCTTTGATTTTTCTAGCTCCAGCATGAAGGATATCCTTGGAAAATATAAGTTGCAGTCTGCTAACCTTGACAAAGTTGAC CAACCTTCTCTTGACTTACAGCTAGAGTAAACGTAGATATTGACTTGATAAATAATAATTATTTATGAGCTGAGGCAGAT GAAAGGTGAGGAACTTGAAGGATTGAGTTTAGAAGAATTACAACAAATTGAGAAAAGACTTGAAGCTGGATTCAACCGTG TGCTTGAGATTAAGGGTACAAGAATTATGGATGAAATTGCCAACCTTCAAAGAAAGGGTGCTGAGCTGATGGAAGAAAAC AAAAAATTGAAACATAAAATGGAAATGATGAAGTTAGGAAAGTTTCCCTTACTAACAGACATGGACTGCATGGTTATGGA AGAAGGCCAATCATCTGACTCTATAATTACCACCAATAATGTTTGCTCTAGTAATAGTGGCCCTCCTCCTGAAGATGATT CCTCAAATGCTTCTTTGAAGTTAGGTTGTAACAATGGTCTAGCACCTGTGGATGATGATTGCTCAATTACATCTTTGAAG TTAGGGTTTACTTCTAACCTTCTGATTGTTTTTGAGTAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| MPF2-sgRNA1 | Physalis pruinosa | MPF2-sgRNA1 | MPF2-sgRNA1 | Ppr-mpf2CR-a2 (T0 ) |
+45 bp and -54 bp
Is tDNA present:
Mutation sequence: ATGGCAAGAGAGAAGATCAAGATAAAGAAGATCGACAATATAACAGCAAGACAAGTTACATTTTCAAAGAGGAGAAGAGG ACTTTTCAAGAAAGCTGAAGAGCTATCTGTTCTCTGTGATGCTGATGTTGCTCTTATCATTTTCTCTTCTACTGGAAAAC TCTTTGATTTTTCTAGCTCCAGCATGAAGGATATCCTTGGAAAATATAAGTTGCAGTCTGCTAACCTTGACAAAGTTGAC CAACCTTCTCTTGACTTACAGCTAGAGTAAACGTAGATATTGACTTGATAAATAATAATTATTTATGAGCTGAGGCAGAT GAAAGGTGAGGAACTTGAAGGATTGAGTTTAGAAGAATTACAACAAATTGAGAAAAGACTTGAAGCTGGATTCAACCGTG TGCTTGAGATTAAGGGTACAAGAATTATGGATGAAATTGCCAACCTTCAAAGAAAGGGTGCTGAGCTGATGGAAGAAAAC AAAAAATTGAAACATAAAATGGAAATGATGAAGTTAGGAAAGTTTCCCTTACTAACAGACATGGACTGCATGGTTATGGA AGAAGGCCAATCATCTGACTCTATAATTACCACCAATAATGTTTGCTCTAGTAATAGTGGCCCTCCTCCTGAAGATGATT CCTCAAATGCTTCTTTGAAGTTAGGTTGTAACAATGGTCTAGCACCTGTGGATGATGATTGCTCAATTACATCTTTGAAG TTAGGGTTTACTTCTAACCTTCTGATTGTTTTTGAGTAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
