| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a1 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTTGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTT-TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a1 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTTGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTT-TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a1 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTTGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTT-TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a10 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGC
TCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCC
TTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTG
ATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGA
TCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT
ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAAC
TCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACAC
CATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGG
TACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAA
GCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT
TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGG
CAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTC
CCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2050
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2051 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2100
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2101 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2150
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2151 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2200
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2201 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2250
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2251 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2300
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2301 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2350
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2351 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2400
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2401 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2450
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2451 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2500
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2501 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2550
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2551 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2600
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2601 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2650
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2651 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2700
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2701 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2750
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2751 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2800
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2801 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2850
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2851 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2900
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2901 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2950
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2951 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 3000
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3001 TTACTGTTGA 3010
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a10 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGC
TCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCC
TTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTG
ATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGA
TCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT
ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAAC
TCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACAC
CATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGG
TACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAA
GCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT
TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGG
CAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTC
CCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2050
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2051 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2100
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2101 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2150
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2151 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2200
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2201 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2250
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2251 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2300
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2301 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2350
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2351 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2400
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2401 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2450
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2451 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2500
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2501 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2550
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2551 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2600
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2601 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2650
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2651 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2700
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2701 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2750
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2751 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2800
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2801 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2850
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2851 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2900
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2901 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2950
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2951 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 3000
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3001 TTACTGTTGA 3010
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a10 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGC
TCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCC
TTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTG
ATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGA
TCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT
ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAAC
TCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACAC
CATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGG
TACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAA
GCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT
TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGG
CAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTC
CCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2050
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2051 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2100
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2101 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2150
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2151 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2200
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2201 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2250
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2251 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2300
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2301 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2350
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2351 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2400
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2401 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2450
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2451 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2500
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2501 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2550
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2551 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2600
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2601 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2650
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2651 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2700
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2701 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2750
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2751 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2800
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2801 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2850
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2851 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2900
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2901 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2950
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2951 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 3000
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3001 TTACTGTTGA 3010
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a2 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||||| |||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTA-GTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a2 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||||| |||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTA-GTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a2 (T0 ) |
-1 bp and -5 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||||| |||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTA-GTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAA---- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a3 (T0 ) |
-17 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCT
TTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAA
CTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC
AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAAT
TTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCT
CTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCG
TCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAG
GAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAG
AATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGG
CATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCG
TCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGT
AATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACC
ATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGA
TGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACG
TTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACG
AGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||
Mutation 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTT------------ 2038
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 2039 -----TACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2083
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2084 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2133
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2134 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2183
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2184 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2233
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2234 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2283
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2284 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2333
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2334 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2383
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2384 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2433
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2434 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2483
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2484 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2533
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2534 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2583
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2584 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2633
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2634 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2683
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2684 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2733
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2734 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2783
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2784 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2833
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2834 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2883
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2884 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2933
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2934 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2983
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2984 TACTGTTGA 2992
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a3 (T0 ) |
-17 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCT
TTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAA
CTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC
AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAAT
TTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCT
CTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCG
TCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAG
GAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAG
AATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGG
CATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCG
TCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGT
AATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACC
ATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGA
TGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACG
TTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACG
AGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||
Mutation 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTT------------ 2038
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 2039 -----TACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2083
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2084 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2133
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2134 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2183
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2184 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2233
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2234 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2283
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2284 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2333
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2334 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2383
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2384 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2433
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2434 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2483
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2484 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2533
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2534 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2583
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2584 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2633
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2634 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2683
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2684 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2733
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2734 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2783
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2784 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2833
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2834 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2883
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2884 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2933
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2934 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2983
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2984 TACTGTTGA 2992
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a3 (T0 ) |
-17 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCT
TTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAA
CTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC
AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAAT
TTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCT
CTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCG
TCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAG
GAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAG
AATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGG
CATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCG
TCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGT
AATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACC
ATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGA
TGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACG
TTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACG
AGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||
Mutation 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTT------------ 2038
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 2039 -----TACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2083
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2084 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2133
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2134 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2183
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2184 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2233
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2234 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2283
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2284 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2333
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2334 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2383
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2384 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2433
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2434 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2483
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2484 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2533
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2534 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2583
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2584 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2633
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2634 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2683
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2684 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2733
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2734 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2783
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2784 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2833
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2834 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2883
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2884 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2933
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2934 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2983
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2984 TACTGTTGA 2992
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a4 (T0 ) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTATGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a4 (T0 ) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTATGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a4 (T0 ) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTATGTCAAAGGTGTGAGCAATTGGGCATCTT
TCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAAC
TCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCA
GACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
||||||||| ||||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAG-TATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1849
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1850 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1899
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1900 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1949
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1950 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1999
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2000 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2049
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2099
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2149
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2199
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2249
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2299
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2349
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2399
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2449
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2499
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2549
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2599
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2649
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2699
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2749
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2799
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2849
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2899
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2949
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2999
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 3000 TACTGTTGA 3008
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a5 (T0 ) |
-2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTCA
CAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTC
TCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATG
ATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCC
CAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACA
CAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCC
AAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCAT
ACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTAC
AGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCA
AGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGC
TACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAG
ACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCT
TACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2048
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2098
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2148
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2198
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2248
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2298
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2348
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2398
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2448
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2498
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2548
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2598
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2648
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2698
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2748
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2798
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2848
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2898
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2948
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2998
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2999 TACTGTTGA 3007
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a5 (T0 ) |
-2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTCA
CAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTC
TCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATG
ATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCC
CAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACA
CAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCC
AAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCAT
ACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTAC
AGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCA
AGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGC
TACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAG
ACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCT
TACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2048
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2098
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2148
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2198
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2248
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2298
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2348
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2398
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2448
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2498
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2548
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2598
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2648
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2698
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2748
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2798
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2848
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2898
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2948
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2998
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2999 TACTGTTGA 3007
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a5 (T0 ) |
-2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCTGTTTGTACCCCAACCTTGGCAAAATTAGCTCA
CAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTC
TCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATG
ATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCC
CAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACA
CAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCC
AAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCAT
ACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTAC
AGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCA
AGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGC
TACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAG
ACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCT
TACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2048
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2098
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2148
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2198
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2248
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2298
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2348
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2398
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2448
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2498
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2548
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2598
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2648
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2698
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2748
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2798
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2848
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2898
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2948
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2998
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2999 TACTGTTGA 3007
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a6 (T0 ) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
|||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT--- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a6 (T0 ) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
|||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT--- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a6 (T0 ) |
-2 bp and -4 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTGTCAAAGGTGTGAGCAATTGGGCATCTTT
CTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAAACT
CAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGCAG
ACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGT
TTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTTCTCAA
ATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTT
TAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATCCCAAA
CACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTACACAAA
CTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGC
AATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCATACTA
GGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTT
GTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGCAAGCC
GGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTGCTACA
CATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCAGACTG
TATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCCTTACC
TAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTTATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1850
|||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGT--TGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACAA 1848
Wildtype 1851 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1849 GAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATG 1898
Wildtype 1901 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1899 TTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAAC 1948
Wildtype 1951 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 2000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1949 AATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATGC 1998
Wildtype 2001 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGCT 2050
|||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 AGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT--- 2045
Wildtype 2051 GTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2100
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2046 -TTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTG 2094
Wildtype 2101 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2095 GCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAA 2144
Wildtype 2151 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2145 TTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTA 2194
Wildtype 2201 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2195 TTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAGC 2244
Wildtype 2251 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2245 CCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAA 2294
Wildtype 2301 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2295 GTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAG 2344
Wildtype 2351 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2345 ATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA 2394
Wildtype 2401 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2395 CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAAC 2444
Wildtype 2451 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2445 TCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCAT 2494
Wildtype 2501 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2495 GTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAG 2544
Wildtype 2551 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2545 AAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGC 2594
Wildtype 2601 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2595 AACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTGG 2644
Wildtype 2651 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2645 ATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCAT 2694
Wildtype 2701 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2695 TGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGA 2744
Wildtype 2751 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2745 CGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT 2794
Wildtype 2801 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2795 GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCT 2844
Wildtype 2851 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2845 TATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTC 2894
Wildtype 2901 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2895 CGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAA 2944
Wildtype 2951 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 3000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2945 AGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTT 2994
Wildtype 3001 TACTGTTGA 3009
|||||||||
Mutation 2995 TACTGTTGA 3003
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a7 (T0 ) |
+1 bp and -57 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATAGT
ACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACA
GTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGA
CGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCA
ACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATT
CTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTT
CCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTT
TCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTC
TTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAG
TCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAAC
TACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTAC
GCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCG
TGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATA-- 1998
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
Mutation 1998 -------------------------------------------------- 1998
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 -----GTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2043
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2044 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2093
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2094 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2143
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2144 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2193
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2194 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2243
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2244 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2293
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2294 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2343
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2344 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2393
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2394 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2443
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2444 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2493
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2494 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2543
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2544 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2593
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2594 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2643
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2644 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2693
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2694 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2743
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2744 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2793
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2794 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2843
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2844 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2893
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2894 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2943
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2944 TTACTGTTGA 2953
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a7 (T0 ) |
+1 bp and -57 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATAGT
ACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACA
GTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGA
CGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCA
ACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATT
CTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTT
CCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTT
TCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTC
TTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAG
TCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAAC
TACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTAC
GCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCG
TGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATA-- 1998
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
Mutation 1998 -------------------------------------------------- 1998
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 -----GTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2043
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2044 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2093
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2094 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2143
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2144 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2193
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2194 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2243
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2244 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2293
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2294 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2343
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2344 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2393
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2394 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2443
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2444 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2493
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2494 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2543
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2544 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2593
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2594 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2643
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2644 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2693
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2694 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2743
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2744 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2793
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2794 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2843
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2844 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2893
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2894 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2943
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2944 TTACTGTTGA 2953
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a7 (T0 ) |
+1 bp and -57 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATAGT
ACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACA
GTTGCATTGTACAATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGA
CGTGACTCATCCACACCCTCTTGATGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCA
ACAAGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATT
CTTGATGATTTTTACGAGGAGCTACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTT
CCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTT
TCGTGGTCGTTCAGAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTC
TTCAACGAAAACATCCTACCAGGTACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAG
TCATTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAAC
TACAAAAACTGGTATACAACCTTTGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTAC
GCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCG
TGCTGCACCACCAAAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATA-- 1998
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
Mutation 1998 -------------------------------------------------- 1998
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
|||||||||||||||||||||||||||||||||||||||||||||
Mutation 1999 -----GTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2043
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2044 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2093
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2094 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2143
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2144 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2193
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2194 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2243
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2244 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2293
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2294 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2343
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2344 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2393
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2394 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2443
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2444 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2493
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2494 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2543
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2544 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2593
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2594 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2643
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2644 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2693
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2694 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2743
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2744 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2793
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2794 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2843
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2844 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2893
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2894 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2943
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2944 TTACTGTTGA 2953
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a8 (T0 ) |
+1 bp and -2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a8 (T0 ) |
+1 bp and -2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a8 (T0 ) |
+1 bp and -2 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATTGTTTGTACCCCAACCTTGGCAAAATTAGCTC
ACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCTT
CTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGAT
GATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGATC
CCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTAC
ACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACTC
CAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACCA
TACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTA
CAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAGC
AAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTTG
CTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGCA
GACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCCC
TTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-- 2048
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2049 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2098
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2099 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2148
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2149 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2198
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2199 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2248
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2249 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2298
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2299 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2348
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2349 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2398
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2399 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2448
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2449 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2498
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2499 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2548
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2549 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2598
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2599 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2648
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2649 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2698
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2699 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2748
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2749 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2798
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2799 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2848
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2849 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2898
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2899 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2948
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2949 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2998
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 2999 TTACTGTTGA 3008
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a9 (T0 ) |
+1 bp and -1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATCTGTTTGTACCCCAACCTTGGCAAAATTAGCT
CACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCT
TCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGA
TGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGAT
CCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA
CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACT
CCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACC
ATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGT
ACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAG
CAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT
GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGC
AGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCC
CTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
|||||||||||||||||||||||||||||||||||||||||||||||| |
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-C 2049
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3000 TTACTGTTGA 3009
|
Developed narrow leaves and unfused petals. |
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a9 (T0 ) |
+1 bp and -1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATCTGTTTGTACCCCAACCTTGGCAAAATTAGCT
CACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCT
TCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGA
TGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGAT
CCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA
CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACT
CCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACC
ATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGT
ACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAG
CAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT
GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGC
AGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCC
CTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
|||||||||||||||||||||||||||||||||||||||||||||||| |
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-C 2049
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3000 TTACTGTTGA 3009
|
|
| AGO7-sgRNA1 |
Physalis pruinosa |
AGO7-sgRNA1 |
AGO7-sgRNA1 |
Ppr-ago7CR-a9 (T0 ) |
+1 bp and -1 bp
Is tDNA present:
Mutation sequence: ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCAATGGATGTTTGCTAGGTCTGTTTCTGATCA
AGTAAAGGAAAAAGTCAAAACTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAATGCCAGACTT
ACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACCTCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTT
CAGAAACCTCCATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTCCAACAAATCTCAGCCTCAAA
GGTGCTCCAAAGACAAAATAATGTGTCCAATCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG
CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCTCTTCTTGCTAATCATTTCCTTGTTCAGTTT
GATCCTTCACAGAGAATCTTCCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGCTTATCAAGAA
AAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCTAGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTA
TCGAATTTCAAAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCAGCAACAAGTCTGGAGAAATG
GTCAAGTTGCAGAAAGCCGGAGGACAGCAGATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA
AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTCCATTTCCTCAGGAGTATTTGCATGCGTTAG
ATGTTGTGTTGCGTGAGAGCCCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTATGGGAGGAGGA
AGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCTTTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAA
TGTTGATTTCTCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAAGCGCCTTGACTTTCTCCGCG
GCATTTCTCACAGGAAAACAAGAGGTTTGACGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC
TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAACCGAGGAAGTTACGGAGAACCTCTGCTTTCA
GGATAGGGATGGAAAGATTCTAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATATAGGAACTTGC
CGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCCGATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTT
GGGAAGTTTTCGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGGGAAAGAAAGGCAATTATAGA
CAGAGTTGTGACAGGACCGGTTGGACCAACAAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA
CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAATCGTGGTCAGGTTAGAGATCTTATTCCTTCT
CGCCATGATAGGCAGTGGAATCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGCTAATGAGTTT
TGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTCATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATC
TTTCTGAACAAGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAATGTTAAAAACTTAGAAACCAA
ACTCAAGAAGTTACATAGAGCTTCATTTAACAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG
CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATCTGTTTGTACCCCAACCTTGGCAAAATTAGCT
CACAGTTTCTGGCAAATTTGGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACAATTCGTTGCCT
TCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTTATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGA
TGATTTTAGCCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACAAGTATGTCTCCAGGATGAGAT
CCCAAACACACAGGCAGGAGATCATTCAAGATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCTA
CACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAACTCAGTTCCTCAAAGTACTTAAAGAGGAACT
CCAAGCAATTCGTGTGGCATGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCAGAAAAGACACC
ATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAGCAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGT
ACAGTTGTGGATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCATTGGGGTGTCAAAGGCACAAG
CAAGCCGGTTCATTACCATGTTCTGTGGGACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTTT
GCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGCTTATTACGCCCATTTAGCTGCATACAGAGGC
AGACTGTATCTTGAACGTTCCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCAAAGACAGTTCC
CTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTTTTACTGTTGA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATGGATGATCGGATTGTGCTTGATAAGGGTCGAAAAAGGCAGTTGCCTCA 50
Wildtype 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 ATGGATGTTTGCTAGGTCTGTTTCTGATCAAGTAAAGGAAAAAGTCAAAA 100
Wildtype 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 CTGATCATGTAGACAACAATAATAATAATAGTAATAATAATCAGAATCAA 150
Wildtype 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 151 TGCCAGACTTACCCTTCTCTTCTCCCTTTACCTCCTCCTGCTATACTACC 200
Wildtype 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 201 TCTGCCTCAAAATCACTACTTCAGATCAAGAACCCACTTTCAGAAACCTC 250
Wildtype 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 251 CATTGAAGCAGAATCATCCTCTTCTTGCCACCCCTTCTCATTCCAACCTC 300
Wildtype 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 301 CAACAAATCTCAGCCTCAAAGGTGCTCCAAAGACAAAATAATGTGTCCAA 350
Wildtype 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 351 TCTTAAAGATGGCAGGAGGGTCAAGGCTGCTACCGCCCTAGAATCTCTCG 400
Wildtype 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 401 CGGTGGCAAGAAGACCGGATTCTGGTGGTGTTGAAGGACCAGTAATATCT 450
Wildtype 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 451 CTTCTTGCTAATCATTTCCTTGTTCAGTTTGATCCTTCACAGAGAATCTT 500
Wildtype 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 501 CCATTATGATGTTGAAGTTTCACCTCATCCATCCAAGGATATTGCTAGGC 550
Wildtype 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 551 TTATCAAGAAAAAACTTGTGGAGGAGCATTCTGTTGTGCTCTCAGAGGCT 600
Wildtype 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 601 AGTCCGGTTTATGATGGCGGAAGGACAATATATAGCCCTATCGAATTTCA 650
Wildtype 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 651 AAGTAATAAGATTGAATTTTACATTAGTCTGCCAATTCCTAGTAGTGGCA 700
Wildtype 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 701 GCAACAAGTCTGGAGAAATGGTCAAGTTGCAGAAAGCCGGAGGACAGCAG 750
Wildtype 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 751 ATTAAGCTTTTCCGCGTTAACATCAAACTTGTATCTAAATTTGATGGGGA 800
Wildtype 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 801 AGAACTAAATAGCTACTTGAATAAAGAAGGAGATGATGGTGGTGGAAGTC 850
Wildtype 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 851 CATTTCCTCAGGAGTATTTGCATGCGTTAGATGTTGTGTTGCGTGAGAGC 900
Wildtype 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 901 CCAACGGAGAAGTGTATAACAGCTGGGAGATCGTTTTACTCGAGTTGTAT 950
Wildtype 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 951 GGGAGGAGGAAGAGATATCGGGGGTGGAGCTGTGGCGCTAAGGGGGTTCT 1000
Wildtype 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1001 TTCAGAGTCTTAGACCAACACAACAAGGGCTTGCTCTCAATGTTGATTTC 1050
Wildtype 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1051 TCGGTGACTGCTTTCCATGAGAGTATTGGAGTGATCTCCTACTTGGAAAA 1100
Wildtype 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1101 GCGCCTTGACTTTCTCCGCGGCATTTCTCACAGGAAAACAAGAGGTTTGA 1150
Wildtype 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1151 CGAATGAAGAGAAGAAAGAGGTGGAGAAAGCACTAAAGAACATTAGAGTC 1200
Wildtype 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1201 TTCGTTTGCCACAGAGAGACTGTTCAGAGATATCGTATTTACAGCTTAAC 1250
Wildtype 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1251 CGAGGAAGTTACGGAGAACCTCTGCTTTCAGGATAGGGATGGAAAGATTC 1300
Wildtype 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1301 TAAGAGTAGTGAACTACTTCAAGGATCATTATAACTATGATATACAATAT 1350
Wildtype 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1351 AGGAACTTGCCGTGCTTGCAGATTAGCAGAAGTAAACCTTGTTATCTTCC 1400
Wildtype 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1401 GATGGAACTTTGTATGATTTGTGAAGGACAGAAGTTTCTTGGGAAGTTTT 1450
Wildtype 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1451 CGGATGATCAGACTGCAAGAATACTCAAAATGGGATGTCAAAAACCAAGG 1500
Wildtype 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1501 GAAAGAAAGGCAATTATAGACAGAGTTGTGACAGGACCGGTTGGACCAAC 1550
Wildtype 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1551 AAGTGGAAATCATGCAAGGGACTTCAAGCTTCAAATCTCGAGAGAAATGA 1600
Wildtype 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1601 CTCGATTGTATGGGAGACTTCTTCAGCCTCCCAAGCTTAAACTAGGGAAT 1650
Wildtype 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1651 CGTGGTCAGGTTAGAGATCTTATTCCTTCTCGCCATGATAGGCAGTGGAA 1700
Wildtype 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1701 TCTTCTAGACAGCCATGTCTTCGAAGGTACTCGAGTTGAGAGATGGACGC 1750
Wildtype 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1751 TAATGAGTTTTGGTGGAACCTCTGATCAAAAGTCCCACATACCGAAATTC 1800
Wildtype 1801 ATAAACCAGTT-ATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1849
||||||||||| ||||||||||||||||||||||||||||||||||||||
Mutation 1801 ATAAACCAGTTAATGTCAAAGGTGTGAGCAATTGGGCATCTTTCTGAACA 1850
Wildtype 1850 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1851 AGAATACAGTACTTAACCCTCAGTTTGAACCCTTGCATCTGCTCAACAAT 1900
Wildtype 1900 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1901 GTTAAAAACTTAGAAACCAAACTCAAGAAGTTACATAGAGCTTCATTTAA 1950
Wildtype 1950 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 1999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1951 CAATCTCCAGCTCGTTATTTGTGTGATGGAGAGAAAACACAAAGGATATG 2000
Wildtype 2000 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAATGC 2049
|||||||||||||||||||||||||||||||||||||||||||||||| |
Mutation 2001 CAGACTTGAAAAGAATCGCTGAGACAGGCATCGGGGTTTTAACCCAAT-C 2049
Wildtype 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2050 TGTTTGTACCCCAACCTTGGCAAAATTAGCTCACAGTTTCTGGCAAATTT 2099
Wildtype 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2100 GGCTCTCAAGATCAATGCCAAAGTTGGGGGATGCACAGTTGCATTGTACA 2149
Wildtype 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2150 ATTCGTTGCCTTCTCAAATACCACGTCTCTTCAAACATGACGGTCCAGTT 2199
Wildtype 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2200 ATTTTTATGGGTGCCGACGTGACTCATCCACACCCTCTTGATGATTTTAG 2249
Wildtype 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2250 CCCCTCCGTCGCTGCTGTAGTTGGTAGTGTGAATTGGCCAGCAGCCAACA 2299
Wildtype 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2300 AGTATGTCTCCAGGATGAGATCCCAAACACACAGGCAGGAGATCATTCAA 2349
Wildtype 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2350 GATCTGAGCATGATGCTCGGGGAGATTCTTGATGATTTTTACGAGGAGCT 2399
Wildtype 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2400 ACACAAACTCCCCGAGAGAATAATCTTCTTCAGGGATGGAGTAAGTGAAA 2449
Wildtype 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2450 CTCAGTTCCTCAAAGTACTTAAAGAGGAACTCCAAGCAATTCGTGTGGCA 2499
Wildtype 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2500 TGTTCGAGATTTCCAGGTTACAAACCTCCTATTACTTTCGTGGTCGTTCA 2549
Wildtype 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2550 GAAAAGACACCATACTAGGTTGTTTCCGTCTGAACTTGATCAGTCATCAG 2599
Wildtype 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2600 CAACTAAAAACACGCTCTTCAACGAAAACATCCTACCAGGTACAGTTGTG 2649
Wildtype 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2650 GATACAGTAATCACTCATCCAAGTGAATTCGACTTCTATCTCTGCAGTCA 2699
Wildtype 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2700 TTGGGGTGTCAAAGGCACAAGCAAGCCGGTTCATTACCATGTTCTGTGGG 2749
Wildtype 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2750 ACGAAAACCAGTTCACTTCTGATGAACTACAAAAACTGGTATACAACCTT 2799
Wildtype 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2800 TGCTACACATTTGTGAGATGCACCAAGCCAATTTCACTCGTGCCCCCGGC 2849
Wildtype 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2850 TTATTACGCCCATTTAGCTGCATACAGAGGCAGACTGTATCTTGAACGTT 2899
Wildtype 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2900 CCGATTTGGCTACTCTAACAAGTTCTAACGTATCTCGTGCTGCACCACCA 2949
Wildtype 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 2950 AAGACAGTTCCCTTACCTAAACTCAACGAGAACATTAAAAAGCTTATGTT 2999
Wildtype 3000 TTACTGTTGA 3009
||||||||||
Mutation 3000 TTACTGTTGA 3009
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