Feature Information
Feature ID: Niben101Scf05245g01007
Feature Type: gene
Name: AGO2 / Argonaute 2
Description: Protein argonaute 2
Links to other Database:
Feature Type: gene
Name: AGO2 / Argonaute 2
Description: Protein argonaute 2
Links to other Database:
Name: Niben101Scf05245g01007
(Exon Intron)
Length: 5210
CATCAACGAGATACACCCTTTTAATCCTTATTGAGAATTTGAAGCTACTACTATAAAAGACCAATTACTGAGCGGTCACCAAGGTCTATTGAACAAACTATATCCATAGAAAAAGAAACATGAAGAGAGAAAATACAAATCACATTGAATAACTGATAAACAGACTTCAAGTAGCTCACACACTTACATAGAAGAAATTC
AGTTTCTGTCTCAGAATACATAAATAAAAGAGAGTCGTAAACTTCAAACACACTGGCAGTGGCCTCAAAAGACCAAAGTAAACACACAAAATAAAACGTC
AAACCATTAACACATCACTTGACAAACTCATTCCTAGAAGTCATACCTCTAGAAGTGAGGACCACATAAAGTAAAAGACTGAATGGCAGTAAAACAAACA
TGAAATCCCATTAGATTCACGCACAAACACCCTGCTAGGTCGATAGAAAGGAGGTTCAAATGCGTTTGGAAATGGGCATCTCCAAAATGTGGACAACCAC
CTCCTCCACCAGTGTGGGAGTGGTGAAGGAGATCAAAAAGCTGGGTTGTCTATCGTCAGACAGAACACTAAAAGTGGCTGTCATCAGACAGAACAGTATG
GGGTACCTGCGATCAGACAAAGAACATTTTGTTCTGCAGCTCATGGTGCAATTTAAAGAATCCTTGTTCAAATGAGGTAGTTGAAGAAGATGACGATGAA
GTTGTGAAGGATGCAGTTGAAGATGCAGGAGACTGCATCTCCATAAGCACCTCTTGGAACATCCGTCCCCGGTAGGCAACAAGGTCAGCATAGTAAACTG
GTGGAACAAGTGAAACAGGTTTTGTGCACCGCGCGAAGGTGAAGCACATGTTGTATATAAGCTTCTGTAAGCGGTCAGAATTGAAGCCATTCTCATCCCA
CAAAACATGATAGTGAGTAGGCTTGCTAGTTCCCAATCCTCCAAAATGGCTGCAAAGATAGAAGTCAAAATCAGATGGATGAACAATTACTGTGTCCACA
ACAGTACCCGGAGGTACATTGCCAGGGCCACCATCAGGAAATAGTCGTGTATGGTGTCTTTTCTGAGCCACAACAAGAGTGATTGCTGGTCGATAGTGAT
CATCGTATATAGCCTTCATCAAATCAACCAGCTCTTCATTAAGTACCATATCAAATTGGCCCTCACTCACACCATCACGGAAAACAACAATTCTGTTTGG
TTTAACCGAGTTGAGTAGAGTGTAAGCATTCAGTAGGTCTGCACACATGCTCCCAAAATTTAGTATCTTCTCAGTCCTGTGGTCCTGAGGACAAACTCTA
GCCGCATATCTATTAGCGGCTGGCCAGTTGACAGTGGCAACAACAGCTGCTATAGATGGAGATGTCACATTCCTTGCAGCAGGATGATTAACATCAGCTC
CAATGAACATCACATTATCTTCACCTCCAAAATTAGGGAGCCTTCCCGTAAGTTCCATATTGCTACCTCCCAATTTTGCGTTAATCTTAATACAAAGGTT
TGCAAGATATTGATCTTGTCCCTTGTTGGCTAGAGATGACAAGCAACATTGCGTTACAACACCAATTTTTATTTCAGAGACCCATTTAAGATATTTGTAT
CCATTGTGCTTTGCTGCCATAACACAAACTATCATTTGTAGTCTGTCCTTGATTTTCTCGTCAGCTGCACGAACCACACCTCTGAGGAGATCTTCAACCT
TTTCAACTGCAGAGAGCTCATACATGCCAGTGAAATGTGTTATGACAGGTTCTTCCATGTTGATCCCTAACTTTTTGCACCGCTCTTTCAATCTAAAGAC
AAATTCATCAGTTCTTAGCCTAAAGTTGGGGTTGCGTTCCTGGGAGCTAAAATCAATCAGAGCCCATCGCTGAAGCGCCTTGCCTTCCACCACAGATTTT
CCAACAAGGTTCCACTGGCATTTATTATCCAGGGGAACTCGACTTAGACCACCTAGCTTCAAATCAGGGGTAGGAAGGATACGACCCGAAACACAGGTCA
TGTTCCGATCAACTCTAATTTCAAAATTACGGGTGACAGCCCTGTTATAATCAAGTGAAAGTTAGGAAAAGAATCAGTAACAACAAATACTTTAACAACA
TATACTATCATATATCACCACATTCACAAAATCATGGAAGCATTGCAAAAACGAATGGACTCACCACTTTATGAAATGTCTAAGCAAGGGAAAAAACCCA
ACACAAGATTAAACACAAGCAAACCATACGATTAACGTGAACAAAAGTTAAACAGACAGCATAACTGACCATAAATTTGTAACAACTGTATCAAATAACA
ATTCAGTACTTTACGAAATGCCAAAGCAACGAGCGAAACACAATTAGAAAATACCCGCGTCATAAGTGAAATGACTAAACACGAGAACATATGCGGATCC
AGGATTTAATTTCTCTGGGTTCAATATTAAAATTATTAAATTATATGGGTTCAAATCTATTATTTTTGTAATTTCAATAAATTTTTACATATAAATTTCT
ACTCCGCGTCGAAAGCTACGGCTTCAATTGAACCTGTAGCTTTCAAGCTGGATCCGATCCGCCACTGCACGAGAAAACCATAGATTTAAAGATCAGAAGT
CCAACATGCAGCTATGCAGCATAACCGGATCACAACTTTGTAACAACTAACAAGTGTATCAAAAACTAAAAACATTTCTTGTGGGGGAGAGCTAGAGCGG
GATCTAGGATTTAAACTCTATGGGTTAAAGACAAAAGGTCATGGGTTCATATCCACTATTCATTTCAATTTCAATGAATTTTTGCATATAAATTTGTACT
GCGCGTCAAAAGTTATGGGTTCAATTGAACCCATTTCTATACGACTACAAAGAAGAAATTCAAGGAAGCATAAAAAACTCATAGAAGGAAATACAAACAG
AAATGAAAGAAATTTAAGGAATTCACATACCCGCATGGCCCATCTTCAGCCCGTACCATTTCACATATTGCCTCCCTTCTCTCTCGTGGTGGAACTAGTG
ATATTTTTTTCATAAACAAGGCTGAATCCTTATCTAAATGCTCCTTAGGAAACCGCTGTCCCTCGATCAAGACACAGAATTCCATTGGGACATAGTTTTT
CTTATTACCTTTTCCAAGATCTAATGAAGGTAAATTTTGGTACCTAATCTCCACCTGATATTTTTCCCTGAAGTAGTCAACAAGAAAAACTTTCTTAGGA
GGATCTTTGCCTTCTGGATCTTCAAGGATAAAATGAAGGTCGCGAGTCTTCTCATCAGTCAGCTTCTTAACAACATATTTCTGACTGCTACGACGATGAG
TTACTTTGACTTTCAAACCAACCAATGCATCCTCTGCATCTCTTCTACGAGTAAAATTATTTTCATTAAACTCTCCAATATATTCCCTCAGGAAATCTAG
CACGGGCACTGCTTTGCGGAATGCCAAGACTGAGTAATCTAGGCACAAGGCAAGCCCTCCAGATGTAGGCTTTAGGCTTTGCTGAAAACCTCTATATGCA
GCAACTCCAAACCCAAAGTCATGTTCAGCCAAGTGCTCATTTGAGTAGAAGCTACGACCTGCAATTATCCTTAACCTAGAAGGATTTTCTTTCATAACCA
AATCCATTCCTTGTAGTACATCACGAGGTATGTATGAGAGGCTTCCACTCAAATATTCTTTCAACTTGCAAAGTTTCAGTTCAGCAACAAACTTGATGGT
AAAGACATACGAGCGTCCCCTACCCTCATCCCCATCTGAGCAGTTCACAGTGAATCGCCCAGTAGGAAGTTGGACAGCACTGAAAATGTTCTTCTCACCA
TCATATGCAGTTTTGTCTATTGGAAATTGACCAGGATTATCAGCAAACAGCTTATCTCTTATCATACGAAGATCAGACTTGTTTATTGACTTCTTCACAG
CCCGGGTCTCATCAGTCATGATTTGCTTGATATCCACATCATAATGCATAATGGTAGACTGAGGATTAAATCTAACAGGAAAATGATTAGCAAGCAGTCT
AATTGACTTGACAGCAACTTTTCCCGTATCAGGTCGTGCAATAGGTACAAGCTTTCCGTTGTTACTTTCCGGAGGAGATGATGTACTCTCTTTTTCTGTA
ATACTCAGCGACTTCAGATCAACTTGAACAGGATCTACACAAAAAGATACAATGCATTTTAAGATTCTTGAACATAAAATGAAAGAACACACCATTTTAT
GGGTACGAGTTAATACCAGAAGACTGAGGAGGAGGAGGACTAGAGCCTGAAGGTGGACCCCACTGGCGAACAGTTGATGCTTCTGAGCTATTGGGTTGCA
CATCCCGCTGCTGCTGGTTTCCACCACCATGTTGTGGTGGCTGCTGCGACGGTGGCCTGACCCAAGCAGTACCACTGCCGCCACTAACAAGTTGCTGCTG
CGGTGGCCGGGCCCAAGCAGTACCACTGCTGCCACTACCATGTTGCTGTGGCTGCTGCGGTGGTGGCCGGGCCCAAGCAGTACCGCCACCTCCACGGCTG
ACCCATGCACCACCACGACCCGGACTTCGTCCAACTGGATGCTGCAGACCCGGCTGATTATACGGAGCTCCACGAGCCTGATACTGACCCGGACCAGATG
CTCTTGGCTGGTTACCCCATTGCTGTGGTGGCTGAATTGGTTGCTGGTTGAATGAACCCATCTGGGAGGGCCCACCCCTTCCGCCACCAATCTGGAACCC
ACCCCTTCCACCTCCACGGCCTTGCTCCTGTTCGTATTGACCACCACCGCGGCCGCGACCACGGTTACCTCCACCACCACCGCCGCGGCTGCTATCACCT
CCACCACCACCACCACCACCACGATTGCTGTCACCACCACCAAGTCGGTAATTTCCACGATCCATTTTCCAACTTCACTAAAGAGAAAAAAAAACTTGAC
TGTAAATTTTCAAAATGAAAAAGCTCAGATGATCTGCAACTGCAAGGATCTAGTAATGTGTACTTATGAAGAGTAAAATGACTGAACACCGTATATAGAG
TGGAGACTAAGGAATGTAAGATGACTAAAAAGGGCTGTCATTTGTAACTAAGAAATCGAAAATGTAAAGAGAAAATGGGAAAATACATAGTGGGGTCGTG
GAAGTTGGAGGATTCTTGATGATTCCAGAAGGATCGATGAAATGGAGGAAACGGTAATTGTAAGATAGGAAGATAACGAGAAAGTGTGAGGAAATGGCGG
ATGATTTTAT
The mutation and phenotype for selected feature: Niben101Scf05245g01007
Project Name:Crispr/Cas9 Mediated Inactivation of Argonaute 2 Reveals its Differential Involvement in Antiviral Responses
RNA silencing constitutes an important antiviral mechanism in plants. Small RNA guided Argonaute proteins fulfill essential role in this process by acting as executors of viral restriction. Plants enc...... Lab: Karoly Fatyol Lab, Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Szent-Gyorgyi Albert u. 4, Godollo, 2100, Hungary.; Contact: Karoly Fatyol; kfatyol@gmail.com| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1 (T0 ) | -5 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1.1 (T1 ) | -5 bp / -7 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1.2 (T1 ) | -5 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1.3 (T1 ) | -5 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1.4 (T1 ) | -5 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-1.5 (T1 ) | +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-2 (T0 ) | -2 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-3 (T0 ) | -27 bp / -44 bp / - 5bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-4 (T0 ) | +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-5 (T0 ) | -6 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-6 (T0 ) | -5 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-7 (T0 ) | -65 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-8 (T0 ) | -5 bp / +1 bp | |
| NbRT0001 | Nb-1 | NbTG0001-1 | NbGR0001-1 | ago2-9 (T0 ) | -2 bp / +1 bp / +59 bp |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
