Plant Crispr Database

Feature Information

Feature ID: Bradi3g51380
Feature Type: gene
Name: BdYDA2 (YODA2) / MAPK kinase kinase
Description: STE_MEKK_ste11_MAP3K.10 - STE kinases include homologs to sterile 7, sterile 11 and sterile 20 from yeast, expressed
Links to other Database:

Sequence & Structure:

The mutation and phenotype for selected feature: Bradi3g51380

Project Name:Conservation and divergence of YODA MAPKKK functionin regulation of grass epidermal patterning

All multicellular organisms must properly pattern cell types to generate functional tissues and organs. The organized and predictable cell lineages of the Brachypodium leaf enabled us to characterize ...... Lab: Bergmann Lab, Department of Biology, Stanford University, Stanford, CA 94305-5020, USA.; Contact: Dominique Bergmann, dbergmann@stanford.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
BdRT0001	Bd21-3	BdTG0001-1	sgRNA_11	bdyda2-1 (T0 )	-1 bp/-1 bp	Guard cells and subsidiary cells are false-colored green and yellow, respectively.
BdRT0001	Bd21-3	BdTG0001-1	sgRNA_11	bdyda2-2 (T0 )	-1 bp Is tDNA present: Mutation sequence: TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTCTCAAAGCCCAAGAACAATATTTCCTGACCA GATTCCAAGTTCAGCTTTTTGGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGCTCCAGTCCAG GTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGATATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGT CCAGAGGTTCACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTGCATAGCGGAAGTGTCTCTCCA TTGCATCCGAGGGCTGGTGGGATGGCACCTGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACAG ATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCGAACAACTCCGCCCCAACAAGTCCTATATCTC GTAGTCCTGGTAGAGCAGAGAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2542 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC 2591 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC 50 Wildtype 2592 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT 2641 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT 100 Wildtype 2642 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC 2691 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC 150 Wildtype 2692 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA 2741 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA 200 Wildtype 2742 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAGTGTT 2791 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| Mutation 201 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAG-GTT 249 gRNA GAGTGTT Wildtype 2792 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG 2841 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG 299 gRNA GAGTGTTCACCAATTCCTAG Wildtype 2842 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC 2891 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC 349 Wildtype 2892 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA 2941 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA 399 Wildtype 2942 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG 2991 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 400 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG 449 Wildtype 2992 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA 3041 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 450 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA 499 Wildtype 3042 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA 3080 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 500 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA 538	Guard cells and subsidiary cells are false-colored green and yellow, respectively.

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

BdRT0001

Bd21-3

BdTG0001-1

sgRNA_11

bdyda2-1 (T0 )

-1 bp/-1 bp

Guard cells and subsidiary cells are false-colored green and yellow, respectively.

BdRT0001

Bd21-3

BdTG0001-1

sgRNA_11

bdyda2-2 (T0 )

-1 bp

Is tDNA present:

Mutation sequence:

TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTCTCAAAGCCCAAGAACAATATTTCCTGACCA
GATTCCAAGTTCAGCTTTTTGGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGCTCCAGTCCAG
GTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGATATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGT
CCAGAGGTTCACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTGCATAGCGGAAGTGTCTCTCCA
TTGCATCCGAGGGCTGGTGGGATGGCACCTGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACAG
ATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCGAACAACTCCGCCCCAACAAGTCCTATATCTC
GTAGTCCTGGTAGAGCAGAGAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2542 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC   2591
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TGCCAGGTTGCTCTGGAGAGTGCTCCAAACAGTTTGATGTCAAGCCCTTC     50

Wildtype        2592 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT   2641
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 TCAAAGCCCAAGAACAATATTTCCTGACCAGATTCCAAGTTCAGCTTTTT    100

Wildtype        2642 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC   2691
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 GGGCAGTGAAGCCTCATGCAGATATAACATTCCTTGGGTCTGGTCAGTGC    150

Wildtype        2692 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA   2741
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 TCCAGTCCAGGTTCAGGGCAAACATCTGGGCATAATTCAGTGGGAGGTGA    200

Wildtype        2742 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAGTGTT   2791
                     |||||||||||||||||||||||||||||||||||||||||||||| |||
Mutation         201 TATGCTAGCTCAGCTCTTCTGGCAGCCTAGCCGAGGCAGTCCAGAG-GTT    249
gRNA                                                            GAGTGTT

Wildtype        2792 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG   2841
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 CACCAATTCCTAGCCCAAGAATGATGAGTCCTGGTCCAAGTTCGAGGGTG    299
gRNA                 GAGTGTTCACCAATTCCTAG

Wildtype        2842 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC   2891
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 CATAGCGGAAGTGTCTCTCCATTGCATCCGAGGGCTGGTGGGATGGCACC    349

Wildtype        2892 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA   2941
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         350 TGAATCTCCAACAAATCGGCATGATGAGGTGAAGAAGAAGCAAACCCACA    399

Wildtype        2942 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG   2991
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         400 GATTGCCGCTTCCACCGTTAAGCATCTCTAATAGTTCCACCTTTTTGCCG    449

Wildtype        2992 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA   3041
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         450 AACAACTCCGCCCCAACAAGTCCTATATCTCGTAGTCCTGGTAGAGCAGA    499

Wildtype        3042 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA   3080
                     |||||||||||||||||||||||||||||||||||||||
Mutation         500 GAATCCACCCAGTCCTGGATCACGTTGGAAGAAGGGAAA    538

Guard cells and subsidiary cells are false-colored green and yellow, respectively.

Feature Information

Sequence & Structure:

Name: Bradi3g51380 (Exon Intron) Length: 12306

The mutation and phenotype for selected feature: Bradi3g51380

Project Name:Conservation and divergence of YODA MAPKKK functionin regulation of grass epidermal patterning

Name: Bradi3g51380 (Exon Intron)
Length: 12306