Feature Information
Feature ID: Manes.08G145200
Feature Type: gene
Name: nCBP-2
Description: novel cap-binding protein
Links to other Database:
Feature Type: gene
Name: nCBP-2
Description: novel cap-binding protein
Links to other Database:
Name: Manes.08G145200
(Exon Intron)
Length: 3298
ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGCATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCAAGGTTCTCTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTC
TATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTTGATTATTTTGCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGA
TTTTTGTATTTTTTTTTTCCTGAAGTGGGTTATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTATTTTTTTGAAACCAT
ATCTAAATCATTTCGTTATTTTTTAAGTTCGAGATCATCACTTGTGGTCTTTAATTTTTAGGTTTTTTTTTGAAAGGACAGGCCTTTAGTAAGTCTTTTA
ATTAATATATAGAATATTAGGATTGAACTTAGATGTCGGGCTTCAAAATATGGGTTGCAACTCTTTTGGAAAAGCTTCACATATGCTTGTTTAGATTATT
AAGAATTTAACCCATTTTTGCTTGTTTAAGTTGGTAGCTCAACACAAACTTGATTTTCAGGTAATCAAATCGAGCTTGGAAAAAGAGCCACTGTCAATTT
TACTTTGTCAATAAAGACTATAAGTATAAATCCATAAGTGCCTAGAATTCAAGCTTTACAATCATGGAAGCAAATGATTCAGTTAATGAAAAGTCTATTT
GCATTGATGGCTGTATTATAATGTATCATTGTTTTATAATTGGATTCAAGAGTTTCATGTCTTTTTGTCTCTTTTATAATCCATATATCGATATTAAGTG
CAATGAATTTTCAGGAAATGCACAAACTATAGCTTATGAACTGTCTGTGGGCCTATACTTTAGGCACCTATAAGGACAAACATTGACAAACGATTCCTGA
GTGGGGAGACTTCAAATGGGAACGTCAAATGACCTTGTTCTAGAGTCTCTAATTGGAAAAAGGTGTAATTGGAGTTTCAAGTTTGGAATTTAAAGATAAT
TGTAGTTGCTTTGGAAACTTGAAGAAAAAAAAAAGAAATAGAAAAAAAAAGCAGAAAGGAAATGTTGTTAAATTTATTTGCTTAAGTAAGGTATAATTAC
AATTTAAATTAAGCATTTTAGATTCTCTTATTCTACAAGGGATGAGAAATATTTTTTGCGACTTTTACAATAGCAATAATGCCCAATTAGTTAATCTTCC
AAGCACGTTCCATACAAGCATACTCTGTCTACAGAATTTAAAGTCCATTTCACTGTTGTGTCACTCAAACAATATGCAAATGGATTGAAACATTGTTCTT
TCTATTTCTCTGCACATATCCTTACTTGCCCCTTTAATACATAAATTATAACCATGACAGTGGAAGCAAGGTCAACTTCTCTCTTTGGAATTGTCAATAT
TTTAGGGGCTTTAGAAGCCTATATCATCAGATCAAACTGTAATGTGCACTATATTACACCACAGTTGTAGTGTTTTACCTTTGCTAATATTGTGGTTGTA
CTCATATCTGTTTTATATGTATAATCCGTAATGGTTTTGTTCCAATGTGCTTACTGGGCTTTCCATGTTTGTGATCAGCACAAGTTTGTATTTTGGTACA
CTCGCCGAACACCAGGAGTTCGAACACAAACTTCATATGAGGATAATATAAAGAAAATTGTGGAGTTCAGTACAGTAAGTTCTATTGTCATTGAACATGT
GGATTACTAGTTTAAGTTATGTACTCACTTAATTACAAGATGTTTTCCATGGTTCATTTTGCTAACCGGATCTTTGGCATTGCTTTATATAGGTTGAAGG
CTTTTGGGTCTGCTATTGCCACTTGGCTCGGCCTTCATCTTTGCCTAGTCCCACTGATCTACATCTCTTCAAGGAGGGAATCCGTCCTCTATGGGAGGTC
AAGTCATAAGCCTAACCTTGTGATGTGTTTTGGTTACCTGTTGTTTTGGTTGATAACTGCTGACAAATATTCTTATTTTTATGCTAAGCACAAGTAGTTT
TAACATTTTATCTATCAGGATTCTGCAAACTCCAATGGAGGCAAGTGGATAATACGATTCAAAAAAGTTGTCTCTGGTCGCTTTTGGGAGGACATGGTAA
GAGAAAAAGCTGAAATGAATATGTTTGTTGTTTGCAATATCTTTGCCCTTTGCTATTGCCTTTGACAATTTAGTGTTGCTTGAAAGTTTGCTTCATTTGT
TGTTAAGATTTTGGTTCACCTCAGTATTCCCTATTTGTCCGACTGTTGCAGTTTTTGGAATCCCCAGTGATGTGCATACCAACTTACTTTGCATCACTAT
TTTATTTTGACACTTGCACTTGCTGCATTATATTTCAACTGATTTAGATTATTTTATCTAGAATTTCATATAGAACTAGTCAAAACCTCTACCTGTGGGT
GCTTGATTATATGGCAGAAAGATGCTGCGTGGTAGGTACTGTTTACAGTTTTGACGCTGGGTAAATTTCTGTACTAGTTAGATGCATTCCACGTCACATT
TTAGTTGCATCATGTGGCCCTCTTTTAGTCCACGCTCAGGAAAATTGTAAGGTTTAACCACTATAGTGTTGGCTTTTCCACTAAATTAGAAATGCTGGAA
TGGATCTTTATTATTTACTTTCACATGCAAATGCAGGTGCTTGCCTTAGTGGGTGACCAACTTGATTATGGTGATAACATATGCGGTGCAGTACTAAGCA
TTCGTTTTAATGAAGATATACTGAGTGTTTGGAATCGTAATTCCTCTGATCATCAGGTCTGCAATTACTGTTTACTAATTATTCTGTTCCCTCCTTTGGT
GCACTCTATGCAACAACCCTTATTATTGCTTTTATGTTACATTAGGCTGTGATGGCTCTAAGGGATTCGATCAAAAGGCACTTAAAGCTTCCCCACAGCT
ACGTTATGGAATACAAGCCCCATGATGCGTCTTTGCGTGACAACTCATCATATAGAAACACATGGTTGAGAGGATAAAAGTATCAGCGGTTATGAAGCTG
GATGAGCAATTGAAATGTAGAGGGTCCTTTCCGGGTCCCTGCTTCATACTTCTAGTTCATTCCCTGATCACGCGATCCTTCGGATGTCAAGTTTACAATT
TAACTTGTGTGGTTCAATTAGTATCCTGTAGGTTTTAATGATACTGGCTGAAACAAAATGTTGCTTTCAATGAAATACCAGACTTGGGTTTGGATGTT
The mutation and phenotype for selected feature: Manes.08G145200
Project Name:Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and Central Africa and threatens production in West Africa. CBSD is caused by two species of positive-sense RNA viru...... Lab: Rebecca Bart, Donald Danforth Plant Science Center; Contact: rbart@danforthcenter.org| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| nCBP-1 | 60444 | nCBP-1 | nCBP2-sgRNA | ncbp-1/2 #2 (T0 ) |
-11 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGC ATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCTTTCGCT CTTTTATGTGTCTATCTGTTGTTTAATTCTATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTTGATTATTTTG CTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGATTTTTGTATTTTTTTTTTCCTGAAGTGGGTT ATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
| nCBP-1 | 60444 | nCBP-1 | nCBP2-sgRNA | ncbp-1/2 #8 (T0 ) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGC ATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCAGGTTCT CTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTCTATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTT GATTATTTTGCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGATTTTTGTATTTTTTTTTTCCT GAAGTGGGTTATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
| nCBP-1 | 60444 | nCBP-1 | nCBP2-sgRNA | ncbp-2 #6 (T0 ) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGC ATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCAGGTTCT CTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTCTATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTT GATTATTTTGCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGATTTTTGTATTTTTTTTTTCCT GAAGTGGGTTATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Symptom incidence development similar to wild-type |
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