Feature Information

Feature ID: Manes.09G140300
Feature Type: gene
Name: nCBP-1
Description: novel cap-binding protein
Links to other Database:

The mutation and phenotype for selected feature: Manes.09G140300

Project Name:Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence

Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and Central Africa and threatens production in West Africa. CBSD is caused by two species of positive-sense RNA viru...... Lab: Rebecca Bart, Donald Danforth Plant Science Center; Contact: rbart@danforthcenter.org
Transformation Genetic background Construct gRNA Plant Mutation Phenotype
nCBP-1 60444 nCBP-1 nCBP1-sgRNA ncbp-1 #1 (T0 ) -3 bp Symptom incidence development similar to wild-type
nCBP-1 60444 nCBP-1 nCBP1-sgRNA ncbp-1/2 #2 (T0 ) +1 bp Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV).
nCBP-1 60444 nCBP-1 nCBP1-sgRNA ncbp-1/2 #8 (T0 ) -1 bp and -3 bp Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV).



This database is supported by NSF (IOS-1546625) and hosted by BTI.