Feature Information
Feature ID: Manes.09G140300
Feature Type: gene
Name: nCBP-1
Description: novel cap-binding protein
Links to other Database:
Feature Type: gene
Name: nCBP-1
Description: novel cap-binding protein
Links to other Database:
Name: Manes.09G140300
(Exon Intron)
Length: 3861
TAAGAACTAACTATTATTATTATAAATTCATGTTGCATGCTTTTCTTACAACTCAATCCCACACAATTTAAGCTGTGAACCAAACCAGTAAAATTCAGAATCAAAATTAATCAAACAGATCTAACTTATAAACCAAACCGAGATTGTTTAGTAGAGGTACCAATTGCGAACTTTTCCTCCTCAAAAAATTATTTAACAAA
CAAAAAAAATGTATAATAAATTTATTTTAATTTGTTTACTATAGAAACTTAAAACAGCTGTAACTACTTAAATCGAAACCGGTTTGAATTGTTAAATCAA
AGAACCAGTGGTTTGGGCTTGAAGCAGTGATTTCAATCCAAAACCGAATCGTGGCCACTTTTAAAATATAACCACCATATTCAGACCCATTGCCTCAGAT
CTTTTACTGGTTTAACTACATCTGCCATGTACTAGAAACATTATCCTGAAATGGGCAACATCATGCCTTTCAGACAAAACTAGTAAAAGAATTTGGAACG
TTTTCAAGAAAGTAGAAAACATTAACAACAATGGATTAGAGGCAGAGCCTCTCACAAGGTAAGGAAGAGGTGTTTCCCACTTTTTCAAAGAAAAATGAGA
AACGGCCATATTCATTCTTGCGGAGACGAGAAACTTGCGCCTAGATCTAACAAGCATTGCATTTTATCTATAATCATCTAACAAGACAGGCAACATAGTG
AACAAATGTAACTTTCAATGCCGAATAAGGTATTGCATTAAACTCACATTTGTCCAGCCAGTATGACTAAATCCTACAGGATATCAAGTCAATCAGCGGA
CCCATAAGTTGAAAGCTTGAGATATGAAAGGATTGCTTGATCAGGGAATGAACTGTAAATTGAAGCAAGGGTCAGGAAAGAAGCCTCTGCACCTCGATTG
CTCATCCAGCTTCATAATCCAGGATATTTTAACCTCTCAACCAAGTGTTTCTATATGATGAGTTGTCGCGCAAAGAAGCATCATGGGGCTTGTATTCCAT
GACATAGTTGTGGGGAAGCTTTAAATGCCTTTTAAGCGAATCTCTTAGAGCCATCACAGCCTATGTGGCATACAAACAATAAAAATGGTTGTTGCATTAC
ATTTTCAGATAGAATGCAAATAGGGAAGGATGGACAAAAAAAAGCCAATAACAACAGACCTGATGGTCAGATGCATTACGATTCCAAATACTCAGTATAT
CCTCGTTAAAACGAATGCTTAGTACTGCACCACATACATTATCACTGTAACCAAGTTGGTCACCCACTAAGGCAAGCACCTGTATTTGCATGTGAAAGCA
AATAACAAATATCAATCCCAAACTTTCTAAAGTAATGGAAAAGCCACCATTGAAACACCATAATCTTAGCTTGTAACTTTCATGAGTGAGGAACAAAAGA
GGACCACATAATGCAATTGAAAAGTGACATCACATGCATCTAACTTGTATAGAAACTTACAAAGTCTCAACTATTACCTGTTACTATTATATGGCATCCT
TCTACCAAACAGCCAAACACCCACAGGTAGAAGTTGACTTATTCTATATGAATTATAAATAAAATAACTTAAATTAGATGAAATCTAATGCAGCACATGT
CAAAAATAAAATAGCAGCACAATGAACGTGTTCATCTGCACAGCCGCTATCTTCCGAACAGTCAAACACAAATTACAGGCCTTCAGTAAGGATCCTTATA
ACAAAGGAAGCAACATTTCAAGAAGCATTACACTGTCAAAAACAATAACAAAGCAACAAACAAATCATTTCAACTTTTTCTCTTACCAGATCCTCCCAAA
AGCGACCTGAGACAGCCTTTTTGAATCGTATTATCCACTTGCCACCATTGGAGTTTGCAGAATCCTGACAGAGAAATTTTAAAACTACGTGTGTTATATT
TGGCATAATATGAGAATATATGTATGCAATAATGGAATTACCACAACTAAAATAAAAGGGGACCAAAATGGATCAGAAAGTTAGGTTTATGATGAGACCT
CCCATAAAGGACGGATACCCTCCTTGAAAAGATGGAGATCAGTTGGAATAGGCAAAGAAGAAGGTCGAGCCAAGTGGCAATAGCAAACCCAAAAGCCTTC
AACCTATAGAGAACAATGCTAAAGGTCCAGTTAGCAAACTGAACCACGAAAAAAGTCTTGTATTTAAGCAAGAATTTAAATCAAACCAGTATTTACAAAA
GTTCAATGGTGATTGAACTTACTGTACTGAACTCCATGATTTTCTTTATATTCTCCTCATATGATGTCTGTGTTCGAACTCCTGGTGTACGGCGAGTGTA
CAAAAATACAAACTTGTGCTGATCACAGATATGCAAAAGCCCAATAAGCACTTCTATAACAAAGCAATAATAAATTCAATGTGTAGAAAAAAAAACACCA
TGCTATTAGTAAAGATGAAAGGCTCCATGTGGGCAGTAATATAATGCACATTACAGTTTGATGGCATGCGCTTCTGCAGCCCTAAATTAAAGTAGCGACA
ACTCCAAAATGAGCTTACTTTCATGGCATGGCTATAATTTATATCTCAGGAAGGGCAATTAAAGGAGGTGGGGAGAGAGAAAAGGAACATGCTTTCTCCA
CCCACAGATTTCCTTAGTGGCTCAACAATGAAATGGTCTTCAAATTCCATAGATACACATGCTTGGGAGATTAACTAATGCACATAGCTGCTATTGTAAA
AGTGGCATATAATTAAAAACTCTGATGCCTTGTAGATTAATAATCTAAAACGCTTAATTTCAAATATGTGTATAACTAACCTAAGCAAATAACTGTTAAA
AATATTAACTTTGCCTTCAGAAAAGGGGGAAAATTTCTTTCTTCAAGTTTCCAAATTAACTATATGTATTTTCAACTTGAACCTCCTGTTAGGCATTTCA
CCAATTTCACAAACAAGGTCATTCAAAGTTCCCATTTGCCTTTTTCCCCACCCAGTTAAAGATCATACATCAATGTTTGTACTTTCCAGTGCCTAAAGTC
CATGCACAAAGGTGGTTCAGGAATTAACTGTGTTTCTTAAGAACTCATTGCACCAAATGAAAATATGTGGATTTTAAAAAGAGACATAAAGACCTAAAAA
CTCCTGAATTCAAGTATAAAACAATAATACAAGATAATAGAGCATGCTTTTCCACAATCTACTTCTAACCAATCTTCTAAAGCCCGAATACATTTAAGCT
CAATCTTGCCTTATACATCAATTAAAATACCTATCAAAATTTTTCCTTTCAAACCCAAATATAAAAACTAAAACCCTAAATCCCGCTAAATTGACCATCT
CAAACTTATGAATCACGATATAATTTGGCTAGCATTTCAAAAACAACATATCAAAATTCTTTCTTATTGTAACATAACCGCAGATACAACCACTTAAAAA
TAATTAACAAACAGATTCACCGAATGACATTAATGAATCCCATTAATCTATCGCAAAATAATCAAATTTCAAAAACTGGCAAGGATCGAATGTCTACCTA
CAAAAAATTACACAACACAAACTCAAATAAGGAGAGAGGAAGCGAGAACCTTGAGAGGATGCAAACCAGCTTTGAGGTCCCTTGCTTGGCGTTCTTCGGC
TACTTTATCGATATTATCCGGTGATGATGCAGTTCGAGCATTATTATTGCTATAGTTATTGTTTTCTGTTTCCTCCTCTGTGATCTCCATTTCTTTTAGC
TTCTTTGCTGAACCCCAAATCCTAAAAGCTCTAATAGGAGGTCTCTGTATTCGAGTATAGCTTCCTATGAAGTACAGTTGAGAGCGGAAACCAGAGAGGA
TGACAGGTGAAAACCGTTCACCTCTAGCGCTCTTATTCATCGGTAAAATTATTATTTAATC
The mutation and phenotype for selected feature: Manes.09G140300
Project Name:Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and Central Africa and threatens production in West Africa. CBSD is caused by two species of positive-sense RNA viru...... Lab: Rebecca Bart, Donald Danforth Plant Science Center; Contact: rbart@danforthcenter.org| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| nCBP-1 | 60444 | nCBP-1 | nCBP1-sgRNA | ncbp-1 #1 (T0 ) |
-3 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACAGAGGAGGAAACAGAAAACAATAACTATAGCAATAATAATGCTCGAACTGCATCATCACCGGATAATAT CGATAAAGTAGCCGAAGAACGCCAAGCAAGGGACCTCAAAGCTGGTTTGCATCCTCTCATTCTCGCTTCCTCTCTCCTTA TTTGAGTTTGTGTTGTGTAATTTTTTGTAGGTAGACATTCGATCCTTGCCAGTTTTTGAAATTTGATTATTTTGCGATAG ATTAATGGGATTCATTAATGTCATTCGGTGAATCTGTTTGTTAATTATTTTTAAGTGGTTGTATCTGCGGTTATGTTACA ATAAGAAAGAATTTTGATATGTTGTTTTTGAAATGCTAGCCAAATTATATCGTGATTCATAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Symptom incidence development similar to wild-type |
| nCBP-1 | 60444 | nCBP-1 | nCBP1-sgRNA | ncbp-1/2 #2 (T0 ) |
+1 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACAGAGGAGGAAACAGAAAACAATAACTATAGCAATAATAATGCTCGAACTGCATCATCACCGGATAATAT CGATAAAGTAGCCGAAGAACGCCAAGCAAGGGACCTCAAAGCTGGTTTGCATCCTCTCAAAGGTTCTCGCTTCCTCTCTC CTTATTTGAGTTTGTGTTGTGTAATTTTTTGTAGGTAGACATTCGATCCTTGCCAGTTTTTGAAATTTGATTATTTTGCG ATAGATTAATGGGATTCATTAATGTCATTCGGTGAATCTGTTTGTTAATTATTTTTAAGTGGTTGTATCTGCGGTTATGT TACAATAAGAAAGAATTTTGATATGTTGTTTTTGAAATGCTAGCCAAATTATATCGTGATTCATAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
| nCBP-1 | 60444 | nCBP-1 | nCBP1-sgRNA | ncbp-1/2 #8 (T0 ) | -1 bp and -3 bp | Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
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