Feature Information
Feature ID: Medtr4g094545
Feature Type: gene
Name: MtHen1/Small RNA processing gene
Description: small RNA methyltransferase
Links to other Database:
Feature Type: gene
Name: MtHen1/Small RNA processing gene
Description: small RNA methyltransferase
Links to other Database:
Name: Medtr4g094545
(Exon Intron)
Length: 8865
ACCAAACATCGCCATTATCTTTATAATAAAATAATAATAGGATTCTACCATAAAAAACCAACATGTGTATTCTTTTAAACTTCATGTCAGCCTAGGGGAAGACCCTAACTTTTTAACCATCCAGCTCCACAAAATATCTCCCCACATATATCCTAGCAATTTCAGATTCTATCCCTTAACTAAACCTATCAATACTTCTT
TCCCTTATTTCCTAGCATCCATTCTATTTCGGTTGTCTTCTTGCAAGCCCAGCAATCAGAACTATATAAGTCTCATTCTGTTAGCTTCTTTTAAAGAAAA
AAAAAGTTATTTCTTTTTGCTACTCCATTCCCATATTACATTATAATGGCTATCTATATCATCAGTATGCGTCTGGACTTCATCTTCATGACTCCATTCT
CTTTTGAACACAGCAATCTGTGAAGCAAAACCTGGTTCAACATCAGCAAAACCACCAACCCCACTAAACTTCACATTGTAATTGTGCCGTGCAGCCAAGT
CAGATGCCCACTGGTTGAACTGTTCTCTGGTCCACTCAAATTTGTGGTCATTATTGCGAAACTTGCATGACTGTAAGAGGGTTTGCCCGTCCAATTCTTC
TTGTTCTTGGGTAGGGGGATTCGACTTCTGTAGCACTACATTGTATTCAAAATTTGGTGTAGAAACAATGAGAATCTTTGGACAAAAATAACTTAATGCC
ACATTCCCAAAGAGACAAGCTTGATCTTCATCCATATGTTCAATCACCTGCATTATATAATAAATTTATATCGGTTGATTCAGTTCCAAGATAATCAGAG
TTTCTACGCAAATGCAGATTTATGGGTTTAAACATCATGGATGAGCAAAATACTCTGTCCCCTCAAGAACATGACAGTTGTTGCATGTATAAAGTATATT
CCAGCAAATTGAAGCAGAGGTATTTCCAAGTGAAAAGAATGTAGAAGTGCTTGTTCACAGGCTAAAGAGCAAGACCACATCAGCAACATTATCCAGTTTT
TAAGCGTAAAGTGTTTAAACTAAGATATGTTAAAATGAATGCTAGCAAGCATGGGTTTATGTAGAAGTGCTTGTTTACAGGCTAAAGAACGACCATAACA
GCAGCATTATCCAGTTTTTAAGCCTAAAGTATTTAAAAGTGTAAAAATGAATGTTGGCAACATGGGGTGGGTTTATCTCCTCTAAAGTTAGCTAAATTTG
CATGCATGTGGTATGAATTCCTTACTAAAATTTTCATGAGACACTTCCATATTTGCAATTTAAACGAAAGAGCTAAATTGAAATTTTTTGAAGTCACATG
ATTTCCCCTAATCCAAACATCACAATATGAAATCATCTGACACAGTGCAATTTAAAACATCAAACTCAAGTTACAGACAATGAACTTAATCAAAAATGTT
AATTTACGTCAACATTCTATCAAAAAATATGAAGTCAACAATAATAGGAATATAAAATGGATCAAGAAATACCTCTAAACAAGTACCAATATCAAACCCA
TGCAACCTGGAATCAAAATTTGTAATTGAACCTTCGTAAAGAATAACTGACTTTATGTCAATTCTTGTCACACCAGCATCTGGATTTGCATCTAATTTTG
AATTCAGTACCTGCACAATTTATGCACGCAAAATCAAGCAGCTTACAGGCAGAAATTTCACATACATACAAAAACAAAATTATGATTTCAGCTTCCAACA
CAAAGCACTAGAAGGTCCTATAAGACCAAGACCTCCAAGATCAAAAGGCTGCCAGCATCAACTATACATCCAACATAAATTCATTACTTTGAACAATATT
TCCACACTGAGCAGAATTTAAGTCAGTAAAAATCAAGTCAAAGACATCTACTCAAATATTAAAATATCTGCATCAGTTAAATAAGGCACGTACATGATAC
TCAACATAATAATGACAATTGTATGATCAATTACTTTTCTATTTTCAAAAGTTATGGGCAGCATTATTGTTAGGACAAGGATAAAGATGAGTTCGTTAGG
ATATGAGGTTAGAAGTGAGTTAGGGAAGTATTAGGTAGTGGGGTAGTTAAATTATCGTGTGTATGGTTTTGTGTAGAGAAGGTAAGGATTCATATTTGTT
AATGTTTGGTGAATAGAGCAGTAGCTCTATTGTAGAGAGTGGGAAACCCTTGGAGGAGAGTTTTCTCTCTGATTCTTTCTTATTTTCCCACTAAAAGTGT
TCTTTCATTAATTCTTTTAAATTCAATTCTTGTGTTCCTAGCAATTATGCACAAAGACTACATAATACAAGATGGACGTAATAAACACCTCAAGATCTTG
ATAGAGTTGGGACAATGATGGGACACCTAGGCAAGGCACATTCTAGAAGATTGCAGAAGCATAGGAAATAAGAGTTAGTTGGCACTTTTGTTGATTTTAG
GGAATATTCTATTATAAAAGGGAGGGGAGTGTAGTGAGGAATGCATTGAGATTGTTAGAAGTTGGTTAGGAAAGATTTCTCTCAGGTTAGGAGCATTGTG
CTCTGGTAGGAGTGTTCACACTCCGGTTTATCCTTTCTTTTCTATTATTCCACCATTTTAGAGCCTTTGAGCTCACCTTTATCATTCAATAAAAAACTGT
TCCGTTATCTTGAATTCACGGTCAGACAGAAGTCATTACTTTGACATATTTAGGATCAATCTTGTTCAGACAGCGCTCCCTGGTATTATATATTCCAAAC
AATAGAAGACACTTTTTTTCTTGCACATCTAAGCCTAAACCCTAAGTATTCTACTTAAAAAAAAAAAAACTAAAATGCAAATTTTGAGATAGGAAAGTGC
ATGTAAACCTTTGCAGCCCGTGTAAGACCCTTTTGTGAAATATCAACACCAGCAATTTTCTCCAGAGATGTTGTGTAATTTAGCAATGCTTCCAACAAGC
TTCCAGATCCACATCCAAAATCAAGCTGCAATGAAGGAGAACCTCAAACTCACAACTCGGAGTAACAGAAGAAAATAAGTTGAGAAAGTTAAGCGTACCA
AAGTAGTAGCATGGGATTCTAAAATGTGTTGCACTGCAAATTCCACTCGTTGCTTTGAAAGTGGAGGGCTAAAAAGAGCCTGCTCCATTCTTTCCTCTGG
AGGTTCTGCAACTTTGGTCAAACTTATTTCATATTCCACACAGCAGGCCTCTGAAAAATAAGAATATAAAACCATCAACACAATTTTCAATTTAGAATCA
GCAAATTAGCCGCTTGCAAAACAACGTACATGTGTGGGTGTTTAGGGGGAGAATAACAGGAGAAAGTAACTCACTAGAAGATAAGGACGACATTTTGGCT
GAATCACCAGCTGAAGCAAAAATCAAATCAGAAGTGAGCAAGTTCGTGTTGAAATAAGCACACTGGCCAACAGACATCTGCATCACAACCTCCTCAATGT
ATGAAATCACAGCACCAACCCCAATCTCAAACTCAAACTCATTACAAGATTCAATAACTTCTTTCATATTTTCACCCTCCACAACCAAAGATACTGAATA
ACTAATACAAGGTAAAGATCCATTGCATGGACAGACACCAGAGTCTGGACCTTCAATCTTTAAGTTGCAAACGCCATTTACTAACATGTCTTGTAATGAA
TTTGCACACATTGGCTCAAGTGATTTATTACACTGCATTGCATAAAGATGGCGATTCTGAATGGATTGGCCAATTAGAATCTCTCTGAAAAGGTTTTTGG
AAAAAACGTGGATGCTGAAGTTTCCTGCAGTCTCATAGAGCTGTTCAAAAGGAGCAGTTACACTATTAAAATACTTGTTCAGCCATGATAGAAGTTTCAA
TGAGGCATTCTGAATAGCATCATTCTGCTTCTTGTAACAATCATCAGGGGAACACAAAAGTATTACTTCCGCACACCGAGAAAGCAACTTTATTTCACAT
TTAAACATCCCTGAATCTGATTGCTTTGGACATCCATTTACAGAGGCCCCATTGACACATTCAATCACATCAGTTCCAGAGCCTGCAGCCTTAAAACACG
ACTCTGATGATTCAGTTGGTATTTTGAGAGGATGACTTATAATGGAAAAGAGAGGTTCAGACAGGCGATGCTGGCGACAAAACATACAAAGTATATCCCT
GGGAAGGGAACCCCTCCAGTTAACTCTTGTGGTAAATCTTGAAGGCAACTCAGCTGTGAGTAGTGCCTCTCTAGATAATTTGTAAATGCCCCCTGGAGTC
TTCCCTAGAAGCATCCTGTATAGAAAACCTAGCAATCACTAAATGATGCAGAGTTGAATAATTTTACACAAACAGAAACCGACAAATATTTCTAACCAAC
AAAAAAGACAAATGCTCATCTAAAAGAATTCAACGGATGCAAAAATGGATGGGAGGACAAAACTAGTATACTCTCCAAAAAAGCATCATGCAAACGAGAA
TAAAAGTTGGAAAGAACAGAAAAACCTCCAAGTTAAATAATCTAAGTTTTCTACTTTTGTTATGAAGATTCAGTTTGCAGCAACATCAGAGAGGTCCCTT
TATCTTTCTTAACCTACGCAACCGTGGGATGCCTAATTAAAACCTGGAGAAATTATTTATTAGATAGGTTTTAAGTTAAGACGAGTTAATAGGAAATCTA
CTCACAATTATCAAAAGCAGCAAAGGTTTTTACAGGTTGGTCTCCCAAAGTAAAAATAATTCAGTTGGCCGCAATAATAAAGCAAAAATGCATGACATAA
TAACACCTTTTCACCAAGTATTAAGTTTAGCCAAAGAATGTAATATAAGGTTGATCATTGTACTATTAAAATTTCAATAAACACTTGAATTTAAAGAGAC
CTGTAATATAATCGAACAGTTACATCCTCATAGAAAAGATCTCTAGATTTCCGTGTATATCCGATGCATGCTAAGATTGCATCACCAACTATATCTTGCC
CAGACAAGTATCTTGCCCTTGCATTTAGAGATTCGGTTTCTTTTCCATTTCCCGATACAAAAGACCGATCCAGTAGATATGATTGTTGAGCTGCAAAGAA
CAGTCTGGTCTCTGAAGAAGCTTTGCCTATATTCCTATTGGAGCCACAAAGATCAAGAATAAGTTAGAAAACAATTATCTAGTTTAAGAAAAATCTCTGT
AAATACATCATACCTTGAAATCAGAACATTGGCACCATCTTTTAAACCAAGTTCATTTGCAATGATATCAAGGTAGTACTCTCTTAAAGACATACGGAGA
GTCACAGCTTCAACATATTTTTCCATTGAACTAGGAATGCGTATGGCTTCAACCTGAATGCATTCTTGTGTATCGCCCAATTCCATTAACGACTCTATAA
TATCTTGTGGATATGGACTCAGCTTTCTAATCCAGATATGTTGTTCAGATGTAACAAGAAACTCATTTAACTTGGTAGCAGACCTTACTATATATGGAAG
GACCAAAAACGGATTCGACTCCACCTCAGGATTAATACATTTACACAAACTAAAAATCTTTGCATCACAGACAGCAATGACAGAAATAGGAATTGATCCA
TAATGATCGCTCTTTGTTCTCAAACTAGCCCGAATGTGACCACTGAGCGGATGATCAGATATAAGAAACTGTATAGGTGATGAATGGCAAAGAAAAATGG
TATCAGACTGAATTTAAATTCACTGACACATGTAATATTGGAAAATCATGAAGTCCAAAAAAATAATACTTCCTTTTGACTAATTTTTCAATGTGATGAC
CAGCAAGGGGTAATTGTATATCATATCTTACTATAAGATTAGAGGACCAAAAAACTAAAAGAAAGTAGAAAGATAGAATTCATAAACTTGATGTTTTCAA
CCTATGAATGTCCAACAACGTTCAGAGATCAGACCAGTTCTAAAAGAATTATTTGCTTGGCATCAAACAATCGGGAATGAAGGGAATAAGAATATAGTAA
TTTCTAGTGGTGTATCCTTAATGTTCACTGCAACAAACATTGATGTTCAATAACTTGAAACTAACTACAGACTAAATAGTAAACGGCAACATCAGTGAGG
AATCTGTTCTCAAACTTCATCACCAAATGTCTAAATTACCAGCTCAACCAATAATTGGCTACTTACAGACCAATCTAGCAGATAGGAAAAGAAATCATAA
GGATGGCCAGGTGAAACAGAATTAGAAACCTGAAGGGTCATTGGATCATCCGGCTAAACACAACAAAATGATTTAGGGCGAGTTTGTAGAACAGTTTACT
TAAGCACTACTGTCATATAAGATCTTGTATATAACCTGTTTCTACAATAGAAGGTGTTTATCAAATATGCCAAAAACACAAGATAAGCGCTTATAAGCCA
ACTATCATAAACTATTTCTAAAAATTATTCACACACTTGCATAACCGCTAATGTCACAAGATAAGCTCAAATAAGCCCTTCCAAACACCTCTTATTACAT
AAGAGGGATTGTTGTGCTCTTAATATACTTTATTTTCATAACTGAAAGTAGGCATCATTCAATACACAGAAAAAATTACCTACTTTTTCTGAAAAGATAT
AGGCGATTCGGGCAACTAAACTTTCTTGTGCTTCCTGAGGAGTAAGATCAGTGGTCTCTGTAATGATACCAATCTGCAGGAGAGTAATTCATAGTTGTAA
TATTGTGAAAGTTTAAATGTCATAATCCTCTCAAAACAGGTAAACACTTGAAATAGCAGATATGATACAACACAAAGGAGTCCCTACATAGCAGTAGCAA
TTTTAAAAATAATATGCAGATAGGAAGCATGCATATTGGATAAATTATTACTGTATAATCGGCACTAGGAAACGATCCATTTGCCTATCCTCTGTATTTA
ACATATATAGTTTGAAACAATGAGTCAGTATAAAATACAATTGAAACCCACATTAAAAAAAGCCTCTAAATTACAAGACATTGACCCAAAAACAAAAATC
ATCAGAAAATTTTAAGGGCTTGAAATTCTAGAGAAAATTGCCATTAAAATTGAGCCCATCTGAAAAATCATCAGAAAATTTTAAGGGCTTGAAATTCTAG
TGAAAAAATTGCCATTTCAATTGAGCCCATCTGATCAATATTTGACAATCGTTAGTTGGGCTGAACGAGGACACCAGGAAGAAAATGAAAGTTTTTTTGT
TTTTTGTTTTGAAGAAGCCAAACTAGCCCAGTGAAAATCAAACCTGAGACCTTGAGAGGAGCACACTCCAAGGTCCCAAGCCAGCACCACCAGGTCAACC
CAAGTGGGTTGGAGGAAACGAAAGTTAGTAAATGTCTACCATAGCATTATAAGTTATGTAACATTTCAAATGCATTACTGTATTACTGAAAAGACTCAAC
AAAGTAAAATTAACATACAAGTGTGTGTTTGGCATTGACATCTTCAAATACAAGAAATCAAACTCAACTTTCTACTTAGGCAGTTAAGCTATTGTCATCT
TTACAGCAGGACTAAAATAACAAATAATCATCAATAACTAAATGTAACCAGTAAGTATTTTATCGGTTCAATTCTACCACTTCTTGGCAACTATAAATAA
CTTGTTTGAACCAATAAAGAAATGAACAGGTAAGTAAAAGGATATTCGAGTACAAGCACAACAATTTATTCATTATTATCAATAATTTGCATACTGATTC
GAAATGAGTCATACCTTCTCTATAGCCATCTCCGCAGCAGATTGCTCTGCATCCTTCTTTTTCTTAAAGGTCCCCGACACAACTGTAAGTTCTGGAAGCT
GCAATGTGCAACGGTACAAAGATGGCCTCATCTGAGGAATGCTTAACCCTGGACATTCAGTTTGACAGACCTCTTTCACTTCCTCTACCACATAGCAAGC
ATTTTTGCCAAACTTTTGGTGTATTATAGCCTTCGGTGTAAGGGCTGGTTTTTTCGGCGCAGCCACAGGACGTTCTTTTGTTGCCATGAGAAAGCTCAGA
ATATTTCCAGCGTATTCTTGAACATCAAACCATAAACAATATATCAGCCAAAAATTATGAAGCACTAACAAAAACACAGACATTCTGGACATGACACTGA
CACTTCGACATCGGTAAATATTTGAAAAAATAAAATAAAATTAATTGAATGTAATCACATGTATGTCGTGTCGGACACACCTTCTATAAGAAGTGTCTAG
TAGGCTTAATACACACTACTCAGCAAGCAACTAATTAATTACAAAGGTGACTAATAAAAATCTAGGAATGAACTTATACAAAGGATTAACTAAATATAAA
GCTAATTTATCTTTTAAGCAGCATTTGAAAAAAGAAAAAAAAAATAATAAAAATGAAATAAAGAGTGTAACTTTTAAGTAATACTTGTGAACCCATGCTC
ATTTTCCAGAAATTTTGTAACAATATCTTCAACACACACAAAAAGGAAAAGAAAACGCTTAAACCCTAAAACATAGAAATGAAAAACAAAACAAAAATAT
TAAAAAAAAAAAAAAAACTTTTCAGCAGCATAATGCAAAAAGAGGCATGTTACTACTGTTAATCTGTTGTAATAACGTTGATTTTGATAAAGCAAGAATG
AAGGAGTGAAATTGAAAGCGAGAGAATAAAGCGAGTGTTAGTGGAAAAGGGAAAAAGCGAGAATCGAAATTGTGAAGAAAGTTGAAGAATGTAAAGGAAA
AGAAGGAGAGCAAGAGAATGTACCTTAAGGTTTATTGTTGAAGAATGAATATGAATGAAATTAGGGTTTAGAAAAATGTAGTGGAGTAGCAATTTATAGA
ATGAAATGAAGTGTAAAGGAAAAAGAGTGGAAGACGAAAGAGGGAAAGTTTTAAGAGTGTTTTTAGCACACTCAGACTCAGTCTCACGCCAAAAACGTAC
TGTCTCTGTTCCGTAACGGAATCATTGCTGCTACTAACTTGGAAGTTAAGTTTAAACAAACAAAT
The mutation and phenotype for selected feature: Medtr4g094545
Project Name:CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula
Processing of double-stranded RNA precursors into small RNAs is an essential regulator of gene expression in plant development and stress response. Small RNA processing requires the combined activity ...... Lab: Stupar Lab, Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, USA; Contact: Robert M. Stupar; stup0004@umn.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT222-10 (T0 ) | Heterozygous mutation | |
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT222-12 (T0 ) | Heterozygous mutation | |
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT589-10-1 (T1 ) |
+9 bp
Is tDNA present:
Mutation sequence: TACCACAAGAGTTAACTGGAGGGGTTCCCTTCCCAGGGATATACTTTGTATGTTTTGTCGCCAGCATCGCCTGTCTGAAC CTCTCTTTTCCATTATAAGTCATCCTCTCAAAATACCAACTGAATCATCAGAGTCGTGTTTTAAGGCTGCAGGCTCTGGA ACTGATGTGATTGAATGTGTCAATGGGGCCTCTGTAAATGGATGTCCAAAGCAATCTCGGTGTGCAGGCTCAGGGATGTT TAAATGTGAAATAAAGTTGCTTTCTCGGTGTGCGGAAGTAATACTTTTGTGTTCCCCTGATGATTGTTACAAGAAGCAGA ATGATGCTATTCAGAATGCCTCATTGAAACTTCTATCATGGCTGAACAAGTATTTTAATAGTGTAACTGCTCCTTTTGAA CAGCTCTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : no obvious phenotype changes |
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT589-10-13 (T1 ) |
+9 bp
Is tDNA present:
Mutation sequence: TACCACAAGAGTTAACTGGAGGGGTTCCCTTCCCAGGGATATACTTTGTATGTTTTGTCGCCAGCATCGCCTGTCTGAAC CTCTCTTTTCCATTATAAGTCATCCTCTCAAAATACCAACTGAATCATCAGAGTCGTGTTTTAAGGCTGCAGGCTCTGGA ACTGATGTGATTGAATGTGTCAATGGGGCCTCTGTAAATGGATGTCCAAAGCAATCTCGGTGTGCAGGCTCAGGGATGTT TAAATGTGAAATAAAGTTGCTTTCTCGGTGTGCGGAAGTAATACTTTTGTGTTCCCCTGATGATTGTTACAAGAAGCAGA ATGATGCTATTCAGAATGCCTCATTGAAACTTCTATCATGGCTGAACAAGTATTTTAATAGTGTAACTGCTCCTTTTGAA CAGCTCTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : no obvious phenotype changes |
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT589-12-10 (T1 ) |
-9 bp
Is tDNA present:
Mutation sequence: TACCACAAGAGTTAACTGGAGGGGTTCCCTTCCCAGGGATATACTTTGTATGTTTTGTCGCCAGCATCGCCTGTCTGAAC CTCTCTTTTCCATTATAAGTCATCCTCTCAAAATACCAACTGAATCATCAGAGTCGTGTTTTAAGGCTGCAGGCTCTGGA ACTGATGTGATTGAATGTGTCAATGGGGCCTCTGTAAATGGATGTCCAAAGCCAGGGATGTTTAAATGTGAAATAAAGTT GCTTTCTCGGTGTGCGGAAGTAATACTTTTGTGTTCCCCTGATGATTGTTACAAGAAGCAGAATGATGCTATTCAGAATG CCTCATTGAAACTTCTATCATGGCTGAACAAGTATTTTAATAGTGTAACTGCTCCTTTTGAACAGCTCTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : no obvious phenotype changes |
| MtRT0001 | R108 (HM340) | MtTG0001-1 | Hen1-2 | WPT589-12-13 (T1 ) |
-9 bp
Is tDNA present:
Mutation sequence: TACCACAAGAGTTAACTGGAGGGGTTCCCTTCCCAGGGATATACTTTGTATGTTTTGTCGCCAGCATCGCCTGTCTGAAC CTCTCTTTTCCATTATAAGTCATCCTCTCAAAATACCAACTGAATCATCAGAGTCGTGTTTTAAGGCTGCAGGCTCTGGA ACTGATGTGATTGAATGTGTCAATGGGGCCTCTGTAAATGGATGTCCAAAGCCAGGGATGTTTAAATGTGAAATAAAGTT GCTTTCTCGGTGTGCGGAAGTAATACTTTTGTGTTCCCCTGATGATTGTTACAAGAAGCAGAATGATGCTATTCAGAATG CCTCATTGAAACTTCTATCATGGCTGAACAAGTATTTTAATAGTGTAACTGCTCCTTTTGAACAGCTCTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : no obvious phenotype changes |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
