Feature Information

Feature ID: Solyc01g099520
Feature Type: gene
Name: RALF2
Description: Rapid alkalinization factor 2
Links to other Database: TFGD, TEA, SGN, iTAK

The mutation and phenotype for selected feature: Solyc01g099520

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu
Transformation Genetic background Construct gRNA Plant Mutation Phenotype
CRT0029 RG-PtoR CTG0029-1 CGR0029-1 CRT0029-2 (T0 ) WT 78.6%; -1 bp (5.8%); -2 bp (7.5%); +1 bp (4.2%)
CRT0029 RG-PtoR CTG0029-1 CGR0029-1 CRT0029-5 (T0 ) WT (64.9%); -4 bp (30.4%)
CRT0029 RG-PtoR CTG0029-1 CGR0029-1 CRT0029-5-5 (T1 ) -4 bp (97%); -5 (1.5%)
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-1 (T0 ) -2 bp (45.2%); -5 bp (45.7%)
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-1-1 (T1 ) -2 bp (43.8%); -5 bp (47.3%)
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-1-3 (T1 ) -5 bp/-5 bp
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-3 (T0 ) -2 bp/-2 bp
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-3-4 (T1 ) -2 bp/-2 bp
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-4 (T0 ) -35 bp/+44 bp; TIDE didn't work well
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-4-4 (T1 ) +44 bp and nucleotide substitution
CRT0029 RG-PtoR CTG0029-2 CGR0029-2 CRT0029-4-8 (T1 ) -35 bp/-35 bp



This database is supported by NSF (IOS-1546625) and hosted by BTI.