Name: Solyc01g099520
(Exon Intron)
Length: 883
TGAATTATTGACAGACAAATTCAGTTTACTATAAGCTGTAGTATATAGAAACACAAGAGAAAAAAAATGAAAAAATAATTTTTTTAAAAAAAGAGTTTATGACAACAATCAATACCCATTAAGAGCATTTCCTCATTCGTATACCAAACAAGCCCCATCTCCAAAAGAAAAAAAAATAAAATTAAAAATTCAATATACCA
AGAGAAATACAATTTTTTTTTATGGATCCTGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAACGAGTAATAGCACTGCATCCACGAGTGTAG
GGATTTGCTTGAGCTCCAGGTTTGCAGTTGTAGTAGGAAGCTCCACGGCGAGAACACGGCACACTGTTCTTCTGGAGTGCACCATAGCTGATGTACTTTT
TGGTTGCTAAAATACGCCTGTTTGACTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGCAATACTCCCCTTGCATTCACCGGATCTCGC
CGGCACCATCCAATCGTAGCTACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGAACACAAACAATCAAATACGAAGAAACTCCC
ATTTTTTTTTCCTTGAATTGAAAAAAAAATGAAAGAGAAATTAAAGCAACCTTTAGCTAATTCTTCTGGTTTATGATTCGGACGGCTAGTTTTGCCGTAT
TTTCGATGTTGAAAATTTGTTAGTTTCTGCAAATTGTGAAGAAGACAAGTTATAATGGGCAAGGCCAGTTGTATTATAAGTAGAGGTGGTTGTTTATTTA
CCCCTAATACTTTACTTTATTTCCAAATTGCCACGAAGGATTAGAAGACTTTGATACTTTGGTTTATTGGATGCCCACTAACT
The mutation and phenotype for selected feature: Solyc01g099520
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0029 | RG-PtoR | CTG0029-1 | CGR0029-1 | CRT0029-2 (T0 ) | WT 78.6%; -1 bp (5.8%); -2 bp (7.5%); +1 bp (4.2%) | |
| CRT0029 | RG-PtoR | CTG0029-1 | CGR0029-1 | CRT0029-5 (T0 ) | WT (64.9%); -4 bp (30.4%) | |
| CRT0029 | RG-PtoR | CTG0029-1 | CGR0029-1 | CRT0029-5-5 (T1 ) | -4 bp (97%); -5 (1.5%) | |
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-1 (T0 ) | -2 bp (45.2%); -5 bp (45.7%) | |
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-1-1 (T1 ) | -2 bp (43.8%); -5 bp (47.3%) | |
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-1-3 (T1 ) |
-5 bp/-5 bp
Is tDNA present: No
Mutation sequence: TCTCTTTCATTTTTTTTTCAATTCAAGGAAAAAAAAATGGGAGTTTCTTCGTATTTGATTGTTTGTGTTCTTGTTGGAGC TTTTTTCATTTCCATGGCTGCCGCCGGCGACAGTGGTAGCTACGATTGGATGGTGCCGGCGAGATCCGGTGAATGCAAGG GGAGTATTGCAGAGTGCATGGCTGAAGAAGATGAGTTTGCCCTTGACAGTGAGTCAAACAGGCGTATTTTAGCAACCAAA AAGTACATCAGCTATGGTGCACTCCAGAAGAACAGTGTGCCGTGTTCTCGCCGTGGACTACTACAACTGCAAACCTGGAG CTCAAGCAAATCCCTACACTCGTGGATGCAGTGCTATTACTCGTTGCAGGAGCTAGGTTCTCTATTTTCTCTTCTTCTTC CACAGGATCCATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-3 (T0 ) |
-2 bp/-2 bp
Is tDNA present: Yes
Mutation sequence: TCATAAACCAGAAGAATTAGCTAAAGGTTGCTTTAATTTCTCTTTCATTTTTTTTTCAATTCAAGGAAAAAAAAATGGGA GTTTCTTCGTATTTGATTGTTTGTGTTCTTGTTGGAGCTTTTTTCATTTCCATGGCTGCCGCCGGCGACAGTGGTAGCTA CGATTGGATGGTGCCGGCGAGATCCGGTGAATGCAAGGGGAGTATTGCAGAGTGCATGGCTGAAGAAGATGAGTTTGCCC TTGACAGTGAGTCAAACAGGCGTATTTTAGCAACCAAAAAGTACATCAGCTATGGTGCACTCCAGAAGAACAGTGTGCCG TGTTCTCGCCGTGGCTTCCTACTACAACTGCAAACCTGGAGCTCAAGCAAATCCCTACACTCGTGGATGCAGTGCTATTA CTCGTTGC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-3-4 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: TCATAAACCAGAAGAATTAGCTAAAGGTTGCTTTAATTTCTCTTTCATTTTTTTTTCAATTCAAGGAAAAAAAAATGGGA GTTTCTTCGTATTTGATTGTTTGTGTTCTTGTTGGAGCTTTTTTCATTTCCATGGCTGCCGCCGGCGACAGTGGTAGCTA CGATTGGATGGTGCCGGCGAGATCCGGTGAATGCAAGGGGAGTATTGCAGAGTGCATGGCTGAAGAAGATGAGTTTGCCC TTGACAGTGAGTCAAACAGGCGTATTTTAGCAACCAAAAAGTACATCAGCTATGGTGCACTCCAGAAGAACAGTGTGCCG TGTTCTCGCCGTGGCTTCCTACTACAACTGCAAACCTGGAGCTCAAGCAAATCCCTACACTCGTGGATGCAGTGCTATTA CTCGTTGC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-4 (T0 ) | -35 bp/+44 bp; TIDE didn't work well | |
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-4-4 (T1 ) |
+44 bp and nucleotide substitution
Is tDNA present: No
Mutation sequence: ACAGCCCCATCTCCAAAGAAAAAAAAATAAAATTAAAAATTCAATATACCAAGAGAAATACAATTTTTTTTTATGGATCC TGTGGAAGAAGAAGAGAAAATAGAGAACCTAGCTCCTGCAACGAGTAATAGCACTGCATCCATCGGTTAAAATGTGAAAT TGTTTAGCTACAAGAGGAGTCACAAATGACAAGGTAGAACCGGTATCATGTAAGTCTTATACATCAACAGAGAAGGCCCA CGGCGAGAACACGGCACACTGTTCTTCTGGAGTGCACCATAGCTGATGTACTTTTTGGTTGCTAAAATACGCCTGTTTGA CTCACTGTCAAGGGCAAACTCATCTTCTTCAGCCATGCACTCTGCAATACTCCCCTTGCATTCACCGGATCTCGCCGGCA CCATCCAATCGTAGCTACCACTGTCGCCGGCGGCAGCCATGGAAATGAAAAAAGCTCCAACAAGAACACAAACAATCAAA TACGAAGAAACTCCCATTTTTTTTTCCTTGAATTGAAAAAAAAATGAAAGAGAANTTAANGCANCCTTTAGCTAATTCTT CNGGTTTATGATTCGGACGGCTAGTTTTGCCGTATTTTCNATGTTGAAAATTTGTTAGTTTCTGCAAATTGNGAANAANA CAAGTTATAANGGGCAAGGCCAGTTGTATTATAAGTAGAGGNGGTTGTTTATTTACCCCTAATACTTTACTTTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0029 | RG-PtoR | CTG0029-2 | CGR0029-2 | CRT0029-4-8 (T1 ) |
-35 bp/-35 bp
Is tDNA present: No
Mutation sequence: AAGGTTGCTTTAATTTCTCTTTCATTTTTTTTTCAATTCAAGGAAAAAAAAATGGGAGTTTCTTCGTATTTGATTGTTTG TGTTCTTGTTGGAGCTTTTTTCATTTCCATGGCTGCCGCCGGCGACAGTGGTAGCTACGATTGGATGGTGCCGGCGAGAT CCGGTGAATGCAAGGGGAGTATTGCAGAGTGCATGGCTGAAGAAGATGAGTTTGCCCTTGACAGTGAGTCAAACAGGCGT ATTTTAGCAACCAAAAAGTACATCAGCTATGGTGCACTCCAGAAGAACAGTGTGCAAACCTGGAGCTCAAGCAAATCCCT ACACTCGTGGATGCAGTGCTATTACTCGTTGCAGGAGCTAGGTTCTCTATTTTCTCTTCTTCTTCCACAGGATCCATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
