TGAAAGATCAAAATCAACGGTCTATAACCGATCTTATCCCACAAACCTGCAAAAGCCTTTACGCTTCAGCCGTCAATAAA
TATCAGTCGCCATAACATTACTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGGACGTATAATT
TCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACG
ACACCGTTGCCGGTCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTAGTCAGCGTTCAAATTCCGG
CAAGGGTATGCTTTTGTTGCTATCTTTCGCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATTAT
GATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATATTTATCTGTGAACGTGCACAAATTAGTGC
GATTTAAATAACGCTGTCTATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAACTGGCTAAAA
TATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAGTTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCA
AAAAGAGAAGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTACCGAAAATATTCGGTTCTCA
TGAAGCATTTTTTTTCGTTTTGCTTCAGAATCAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGACGC
GGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGG

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG    150

Wildtype          51 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    200

Wildtype         101 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    250

Wildtype         151 CGGTCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTT    200
                     ||||  ||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 CGGT--CCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTT    298
gRNA                   GTCCCCGGCCAGATAGTGGC

Wildtype         201 TTAGTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTC    250
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         299 TTAGTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTC    348

Wildtype         251 GCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATT    300
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         349 GCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATT    398

Wildtype         301 ATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGT    350
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         399 ATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGT    448

Wildtype         351 ATATTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTC    400
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         449 ATATTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTC    498

Wildtype         401 TATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTT    450
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         499 TATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTT    548

Wildtype         451 AACTGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGC    500
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         549 AACTGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGC    598

Wildtype         501 AAGTTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGA    550
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         599 AAGTTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGA    648

Wildtype         551 AGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGC    600
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         649 AGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGC    698

Wildtype         601 TACCGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAG    650
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         699 TACCGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAG    748

Wildtype         651 AATCAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGAC    700
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         749 AATCAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGAC    798

Wildtype         701 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGG    744
                     ||||||||||||||||||||||||||||||||||||||||||||
Mutation         799 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGG    842

GATCTGAAAGATCAAAATCAACGGTCTATAACCGATCTTATCCCACAAACCTGCAAAAGCCTTTACGCTTCAGCCGTCAA
TAAATATCAGTCGCCATAACATTACTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGGACGTAT
AATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTAACCTGCACCATGAGCTTACTTGACAAGCTCTGG
GACGACACCGTTGCCGGTCTCCTTTACTGAGGAAGCAAGTGAGGACGCGGTGAAGGTGACGAGAAGTATCATGATCGTAA
AGCCTTCCGGGAGTCAGAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTCCTTTTTCTGGT
AAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCA
TTAGAATAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAAAAAAATCTAGAAGAATCTTGTAAG
GTTATTATGGTAAATGTGTAATTTTCTATAATTGACGTAACTTA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         105 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG    154

Wildtype          51 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         155 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    204

Wildtype         101 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         205 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    254

Wildtype         151 CGGTCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTT    200
                     |||||                                             
Mutation         255 CGGTC---------------------------------------------    259
gRNA                   GTCCCCGGCCAGATAGTGGC

Wildtype         201 TTAGTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTC    250
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         251 GCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATT    300
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         301 ATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGT    350
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         351 ATATTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTC    400
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         401 TATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTT    450
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         451 AACTGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGC    500
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         501 AAGTTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGA    550
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         551 AGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGC    600
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         601 TACCGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAG    650
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         651 AATCAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGAC    700
                                            |||||||||||||||||||||||||||
Mutation         260 -----------------------TCCTTTACTGAGGAAGCAAGTGAGGAC    286

Wildtype         701 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         287 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCA    336

Wildtype         751 GAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTAT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         337 GAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTAT    386

Wildtype         801 CTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         387 CTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAA    436

Wildtype         851 CTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAA    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         437 CTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAA    486

Wildtype         901 TAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAA    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         487 TAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAA    536

Wildtype         951 AAAAATCTAGAAGAATCTTGTAAGGTTATTATGGTAAATGTGTAATTTTC   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         537 AAAAATCTAGAAGAATCTTGTAAGGTTATTATGGTAAATGTGTAATTTTC    586

Wildtype        1001 TATAATTGACGTAACTTA   1018
                     ||||||||||||||||||
Mutation         587 TATAATTGACGTAACTTA    604

GATCTGAAAGATCAAAATCAACGGTCTATAACCGATCTTATCCCACAAACCTGCAAAAGCCTTTACGCTTCAGCCGTCAA
TAAATATCAGTCGCCATAACATTACTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGGACGTAT
AATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTAACCTGCACCATGAGCTTACTTGACAAGCTCTGG
GACGACACCGTTGCCGGTCTCCTTTACTGAGGAAGCAAGTGAGGACGCGGTGAAGGTGACGAGAAGTATCATGATCGTAA
AGCCTTCCGGGAGTCAGAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTCCTTTTTCTGGT
AAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCA
TTAGAATAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAAAAAAATCTAGAAGAATCTTGTAAG
GTTATTATGGTAAATGTGTAATTTTCTATAATTGACGTAA

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype           1 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG     50
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         105 CTCACTCCTATGTAGAACACTACAAGCCGAGAAAGTTCTATCCTTGCAGG    154

Wildtype          51 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    100
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         155 ACGTATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCG    204

Wildtype         101 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    150
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         205 CTAACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGC    254

Wildtype         151 CGGTCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTT    200
                     |||||                                             
Mutation         255 CGGTC---------------------------------------------    259
gRNA                   GTCCCCGGCCAGATAGTGGC

Wildtype         201 TTAGTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTC    250
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         251 GCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATT    300
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         301 ATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGT    350
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         351 ATATTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTC    400
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         401 TATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTT    450
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         451 AACTGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGC    500
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         501 AAGTTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGA    550
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         551 AGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGC    600
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         601 TACCGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAG    650
                                                                       
Mutation         259 --------------------------------------------------    259

Wildtype         651 AATCAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGAC    700
                                            |||||||||||||||||||||||||||
Mutation         260 -----------------------TCCTTTACTGAGGAAGCAAGTGAGGAC    286

Wildtype         701 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCA    750
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         287 GCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCA    336

Wildtype         751 GAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTAT    800
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         337 GAATAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTAT    386

Wildtype         801 CTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAA    850
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         387 CTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAA    436

Wildtype         851 CTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAA    900
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         437 CTAGTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAA    486

Wildtype         901 TAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAA    950
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         487 TAATTTGTTCGATTATTAACCGAAAGTACCCTCCAAGAAAAGAGGAGAAA    536

Wildtype         951 AAAAATCTAGAAGAATCTTGTAAGGTTATTATGGTAAATGTGTAATTTTC   1000
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         537 AAAAATCTAGAAGAATCTTGTAAGGTTATTATGGTAAATGTGTAATTTTC    586

Wildtype        1001 TATAATTGACGTAA   1014
                     ||||||||||||||
Mutation         587 TATAATTGACGTAA    600

TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTAACCTGCACCATGAGCTTACTTGACAAGCTC
TGGGACGACACCGTTGCCGGTCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTAGTCAGCGTTC
AAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCCGGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACT
ATCTATTATGATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATATTTATCTGTGAACGTGCACA
AATTAGTGCGATTTAAATAACGCTGTCTATTATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC
TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAGTTACATTTTCCTAAATAAGGACTAGATGAT
AGCTTATCAAAAAGAGAAGGAAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTACCGAAAATATT
CGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAATCAGAAGTTTCAACACCGAGATACTGAGGAAGCAAGTGAGG
ACGCGGTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAATAGAGATTCACCTCCCGTTTCTCCG
GCCGGTACTACTCCTCCGGTATCTCCTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTAGTTCC
TACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAATTTGT

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype          54 TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTA    103
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 TATAATTTCCCTTTTCTCTTCTTCTCCCCTTAACGTGAGCCGATTCGCTA     50

Wildtype         104 ACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGCCGG    153
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ACCTGCACCATGAGCTTACTTGACAAGCTCTGGGACGACACCGTTGCCGG    100

Wildtype         154 TCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTA    203
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCCCCGGCCAGATAGTGGCCTCGGGAAGCTCCGGAAGTATTCTACTTTTA    150

Wildtype         204 GTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCC    253
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GTCAGCGTTCAAATTCCGGCAAGGGTATGCTTTTGTTGCTATCTTTCGCC    200

Wildtype         254 GGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATTATG    303
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 GGCGTTTTCTGAACTCTATTAACAAACAATAATCAGTACTATCTATTATG    250

Wildtype         304 ATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATA    353
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 ATTGGCTTATCAAATCTGTGTTAATCTGAATTATCACTTAACATAGTATA    300

Wildtype         354 TTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTCTAT    403
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 TTTATCTGTGAACGTGCACAAATTAGTGCGATTTAAATAACGCTGTCTAT    350

Wildtype         404 TATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC    453
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         351 TATTTTTTTTCAAGGAGATCTGATTAACTTGATCAGTATTTAAAGTTAAC    400

Wildtype         454 TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAG    503
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         401 TGGCTAAAATATGTCGATTTCTGTCTCAATTTGATTGCTGATATTGCAAG    450

Wildtype         504 TTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGAAGG    553
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         451 TTACATTTTCCTAAATAAGGACTAGATGATAGCTTATCAAAAAGAGAAGG    500

Wildtype         554 AAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTAC    603
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         501 AAACGATTTTGATAAATAATTTAATCCTAGGATTCTGCAATTTTCGCTAC    550

Wildtype         604 CGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAAT    653
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         551 CGAAAATATTCGGTTCTCATGAAGCATTTTTTTTCGTTTTGCTTCAGAAT    600

Wildtype         654 CAGAAGTTTCAACACCGAGATCCTTTACTGAGGAAGCAAGTGAGGACGCG    703
                     ||||||||||||||||||||     |||||||||||||||||||||||||
Mutation         601 CAGAAGTTTCAACACCGAGA-----TACTGAGGAAGCAAGTGAGGACGCG    645
gRNA                                  AGATCCTTTACTGAGGAAGC

Wildtype         704 GTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAA    753
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         646 GTGAAGGTGACGAGAAGTATCATGATCGTAAAGCCTTCCGGGAGTCAGAA    695

Wildtype         754 TAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTC    803
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         696 TAGAGATTCACCTCCCGTTTCTCCGGCCGGTACTACTCCTCCGGTATCTC    745

Wildtype         804 CTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTA    853
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         746 CTTTTTCTGGTAAGCTGCCACTTTCTGTGATTAGTTTTCCGATCTAACTA    795

Wildtype         854 GTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAA    903
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         796 GTTCCTACTTTCCCGTCGTCGCTTCGGCGCTGATTGTACCATTAGAATAA    845

Wildtype         904 TTTGT    908
                     |||||
Mutation         846 TTTGT    850