Plant Crispr Database

Feature Information

Feature ID: Solyc02g031790
Feature Type: gene
Name: LRRXII-RLK
Description: Receptor-like kinase
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc02g031790

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation
CRT0137	M82 sp+	CTG0137-3	CGR0137-3	CRT0137-16 (T0 )	-4 bp/-5 bp
CRT0137	M82 sp+	CTG0137-3	CGR0137-3	CRT0137-16-11 (T1 )	-4 bp/-4 bp Is tDNA present: No Mutation sequence: AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTCAACCTTACCTGAAACCCTGTGGAGCCTTCA AGATCTCTTGGAACTCAATGCATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTAAAGGCTGCGT CAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATATTCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTT TCCATGGCACACAATAAATTAGAAGGGCCTATTCCGAATTCGGGAAAATGGTGGGGTTGGAATTCTTGGATTTTTCTTAC AACAATCTTACTAGTGAAATTCCAAAGTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACAAATT AAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGTCAATCTTTCATTTCCAATTCTGCACTATGTGGTG CTCCTCGGTTTAATGTGTCGCCATGCCTCATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1911 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC 1960 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC 50 Wildtype 1961 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG 2010 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG 100 Wildtype 2011 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA 2060 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA 150 Wildtype 2061 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT 2110 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT 200 Wildtype 2111 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC 2160 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC 250 Wildtype 2161 ACAATAAATTAGAAGGGCCTATTCCGAGTTCATTCGGGAAAATGGTGGGG 2210 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 ACAATAAATTAGAAGGGCCTATTCCGA----ATTCGGGAAAATGGTGGGG 296 gRNA GGCCTATTCCGAGTTCATTC Wildtype 2211 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA 2260 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 297 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA 346 Wildtype 2261 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA 2310 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 347 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA 396 Wildtype 2311 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT 2360 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 397 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT 446 Wildtype 2361 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT 2410 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 447 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT 496 Wildtype 2411 GTCGCCATGCCTCATC 2426 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 497 GTCGCCATGCCTCATC 512
CRT0137	M82 sp+	CTG0137-3	CGR0137-3	CRT0137-16-11-1 (T2 )	-4 bp/-4 bp Is tDNA present: No Mutation sequence: AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTCAACCTTACCTGAAACCCTGTGGAGCCTTCA AGATCTCTTGGAACTCAATGCATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTAAAGGCTGCGT CAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATATTCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTT TCCATGGCACACAATAAATTAGAAGGGCCTATTCCGAATTCGGGAAAATGGTGGGGTTGGAATTCTTGGATTTTTCTTAC AACAATCTTACTAGTGAAATTCCAAAGTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACAAATT AAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGTCAATCTTTCATTTCCAATTCTGCACTATGTGGTG CTCCTCGGTTTAATGTGTCGCCATGCCTCATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1911 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC 1960 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 AATGTTTCTACTTTAAGATATCTTCATTTAGCTTACAACAGATTGACTTC 50 Wildtype 1961 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG 2010 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 AACCTTACCTGAAACCCTGTGGAGCCTTCAAGATCTCTTGGAACTCAATG 100 Wildtype 2011 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA 2060 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 CATCAGCAAATCTGTTGAGCGGGAGTATACCACCTGAGGTTGGTAATCTA 150 Wildtype 2061 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT 2110 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 AAGGCTGCGTCAGTTATCGATCTTTCAAGAAATGATTTTTCTGGTAATAT 200 Wildtype 2111 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC 2160 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 TCCTAGTACTATAGGGGGTCTTCAAAAGTTGATTAGTCTTTCCATGGCAC 250 Wildtype 2161 ACAATAAATTAGAAGGGCCTATTCCGAGTTCATTCGGGAAAATGGTGGGG 2210 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 ACAATAAATTAGAAGGGCCTATTCCGA----ATTCGGGAAAATGGTGGGG 296 gRNA GGCCTATTCCGAGTTCATTC Wildtype 2211 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA 2260 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 297 TTGGAATTCTTGGATTTTTCTTACAACAATCTTACTAGTGAAATTCCAAA 346 Wildtype 2261 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA 2310 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 347 GTCACTTGAAGCACTCTCACATCTCAACTACTTCAACATCTCTTTTAACA 396 Wildtype 2311 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT 2360 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 397 AATTAAGAGGTGAAATTCCTAGTAGTGGTCCATTTGCAAACTTCACCAGT 446 Wildtype 2361 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT 2410 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 447 CAATCTTTCATTTCCAATTCTGCACTATGTGGTGCTCCTCGGTTTAATGT 496 Wildtype 2411 GTCGCCATGCCTCATC 2426 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 497 GTCGCCATGCCTCATC 512
CRT0137	M82 sp+	CTG0137-3	CGR0137-3	CRT0137-16-3 (T1 )	-4 bp/-5 bp

Feature Information

Sequence & Structure:

Name: Solyc02g031790 (Exon Intron) Length: 3562

The mutation and phenotype for selected feature: Solyc02g031790

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc02g031790 (Exon Intron)
Length: 3562