Name: Solyc02g076980
(Exon Intron)
Length: 1379
CACTATATAAACGCCTTGCTACTGAAGACATTAAATGCAAGCTTCTTACAACAATATTCTTTTCCCTTCTACACTAACCATGGCTATGAAAGTTGATTTGATGAATATTTTAATTACACTATTCTTTGTAATTAGCATGTTCAACACTCAAACAAGAGGTCGCAGCCAGCCAAAACTGTCCGTGTCTGAGAGACACGAGC
TGTGGATGTCACGTCATGGACGCGTTTACAAGGACGAAGTAGAAAAAGGAGAACGATTCATGATTTTCAAAGAAAACATGAAATTCATTGAGTCGGTCAA
TAAGGCAGGGAATCTGTCTTACAAGTTAGGGATGAATGAATTTGCAGATATTACTTCACAAGAGTTTTTGGCTAAATTTACTGGATTAAACATACCTAAT
TCATATCTTTCACCTTCTCCAATGTCATCAACGGAATTTAAAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGACTGGAGAGAGTCTG
GGGCTGTCACTCAAGTGAAACATCAAGGTCGATGTGGTAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATTAGGTATTTTTTTT
GTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTGGATCACTAGAAGGAGCCTACAAAATTGCAACAGGCAACTTGATGGAATTCTCTGAACAGGAAC
TTCTCGATTGCACCACCAACAATTATGGCTGCAACGGAGGTTTTATGACGAATGCATTCGATTTTATCATAGAAAATGGTGGAATTTCAAGGGAATCAGA
TTATGAATACCTAGGTCAACAGTACACATGCAGAAGCCAAGAGAAAACAGCAGCAGTGCAAATAAGTAGTTATCAAGTCGTGCCTGAAGGTGAAACATCA
CTATTACAAGCCGTAACTAAACAGCCAGTGTCCATTGGAATAGCTGCTAGCCAAGATTTACAGTTTTACGCGGGAGGAACTTATGACGGAAACTGTGCCG
ATCGAATTAACCATGCTGTAACAGCGATAGGATATGGAACTGATGAGGAAGGTCAGAAATATTGGTTGTTGAAGAATTCATGGGGAACAAGTTGGGGTGA
GAATGGGTATATGAAAATTATAAGAGATTCTGGGGATCCTTCAGGTCTTTGTGATATCGCAAAGATGTCTTCTTATCCAAACATAGCGTAGCATAGATAA
GCAAGCATTTCTTATATGTTGTTGTATTATTAAGAATTGAGGGGAAAAAATATTGATTGTGTACATGCAAAAGCTTTTTGCATGAAGTATTTGTATCTTT
GTATTAAATGGAACTTTATGAAGTTGTTAAAAAGTGTATTGATTATTATCATATGACGAAGAAAAAAGAGTAATTTTGT
The mutation and phenotype for selected feature: Solyc02g076980
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5 (T0 ) | -13 bp (24.5%); -3 bp (20.6%); +1 bp (23.5%); +2 bp (21.4%) | Morphology : Oval cotyledons (wide type: elongated); Stunted growth(Phenotype Image ) Immunity response: More resistance to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-23 (T1 ) | -3 bp/+1 bp | Morphology : Larger leaves; round and smaller fruits (Phenotype Image ) Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-23-4 (T2 ) |
-3 bp/-3 bp
Is tDNA present: No
Mutation sequence: TGAGAGACACGAGCCTGTGGATGTCACGTCATGGACGCGTTTACAAGGACGAAGTAGAAAAAGGAGAACGATTCATGATT TTCAAAGAAAACATGAAATTCATTGAGTCGGTCAATAAGGCAGGGAATCTGTCTTACATAGGGATGAATGAATTTGCAGA TATTACTTCACAAGAGTTTTTGGCTAAATTTACTGGATTAAACATACCTAATTCATATCTTTCACCTTCTCCAATGTCAT CAACGGAATTTAAAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGACTGGAGAGAGTCTGGGGCTGTC ACTCAAGTGAAACATCAAGGTCGATGTGGTAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATTA GGTATTTTTTTTGTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTGGATCACTAGAAGGAGCCTACAAAATTGCAAC AGGCAACTTGATGGAATTCTCTGAACAGGAACTTCTCGATTGCACCACCAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Larger leaves; round and smaller fruits Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-25 (T1 ) | +2 bp/-13bp | Morphology : Larger leaves; round and smaller fruits (Phenotype Image ) Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-25-1 (T2 ) |
-13 bp/-13 bp
Is tDNA present: No
Mutation sequence: GAGAGACACGAGCTGTGGATGTCACGTCATGGACGCGTTTACAAGGACGAAGTAGAAAAAGGAGAACGATTCATGATTTT CAAAGAAAACATGAAATTCATTGAGTCGGTCAATAAGGCAGGGAATCAGGGATGAATGAATTTGCAGATATTACTTCACA AGAGTTTTTGGCTAAATTTACTGGATTAAACATACCTAATTCATATCTTTCACCTTCTCCAATGTCATCAACGGAATTTA AAAAAATTAATGATCTTAGCGATGATTACATGCCGTCTAACTTGGACTGGAGAGAGTCTGGGGCTGTCACTCAAGTGAAA CATCAAGGTCGATGTGGTAAGATAGAACACTGTCTCATGAAATATTATTCACTGATATTAATTAATTAGGTATTTTTTTT GTATTATTAGGATGTTGCTGGGCGTTTTCTGCAGTTGGATCACTAGAAGGAGCCTACAAAATTGCAACAGGCAACTTGAT GGAATTCTCTGAACAGGAACTTCTCGATTGCACCACCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Larger leaves; round and smaller fruits Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-25-10 (T2 ) | +2 bp (43.5%); -13 bp (50.5%) | Morphology : Larger leaves; round and smaller fruits Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-26 (T1 ) | +1 bp/+2 bp | Morphology : Larger leaves; round and smaller fruits (Phenotype Image ) Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-33 (T1 ) | +1 bp/+2 bp/-13 bp | Morphology : Larger leaves; round and smaller fruits (Phenotype Image ) Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-33-5 (T2 ) | +2 bp (78.8%); -13 bp (20.5%) | Morphology : Larger leaves; round and smaller fruits Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-6 (T1 ) | +1 bp/+2 bp/-3 bp | Morphology : Larger leaves; round and smaller fruits (Phenotype Image ) Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-1 | CGR0039-1 | CRT0039-5-6-16 (T2 ) | +1 bp (48.1%); +2 bp (47.4%) | Morphology : Larger leaves; round and smaller fruits Immunity response: More resistant to DC3000dd and T1 strains |
| CRT0039 | RG-PtoR | CTG0039-3 | CGR0039-3 | CRT0039-1 (T0 ) | -1 bp | |
| CRT0039 | RG-PtoR | CTG0039-3 | CGR0039-3 | CRT0039-1-1 (T1 ) | -1 bp | |
| CRT0039 | RG-PtoR | CTG0039-4 | CGR0039-4 | CRT0039-10 (T0 ) | +1 bp 48%; -5 bp 42% | |
| CRT0039 | RG-PtoR | CTG0039-4 | CGR0039-4 | CRT0039-11 (T0 ) | +1 bp | |
| CRT0039 | RG-PtoR | CTG0039-4 | CGR0039-4 | CRT0039-11-2 (T1 ) | +1 bp | |
| CRT0039 | RG-PtoR | CTG0039-4 | CGR0039-4 | CRT0039-6 (T0 ) | -2 bp (47%); -8 bp 46% | |
| CRT0039 | RG-PtoR | CTG0039-4 | CGR0039-4 | CRT0039-6-1 (T1 ) | -8 bp | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-2 (T0 ) | -2 bp 43%; +1 bp 52% | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-2-1 (T1 ) | +1 bp /-2 bp | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-2-2 (T1 ) | -2 bp | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-3 (T0 ) | +1 bp | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-4 (T0 ) | -1 bp 40%; -36 bp 49% | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-7 (T0 ) | +1 bp | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-8 (T0 ) | -13 bp 47%; -3 bp 45% | |
| CRT0039 | RG-PtoR | CTG0039-5 | CGR0039-5 | CRT0039-9 (T0 ) | -1 bp 47%; -5 bp 43% |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
