Name: Solyc02g077040
(Exon Intron)
Length: 1644
TATTCACTCAGCCTCTCTTTTAGTTTTTAACTTAGTTCCTATTTTGATATTTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCAAGAATATTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTATAGTAACTTCCCGCAACTTAAAAGAATTATCCATGCTTGAAAGGCATGAAAATT
GGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTCAAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAA
GAACGGAACTCAACGTTATAAGCTAGCCATCAATAAATATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTA
TCCCAGCAAGAATCAACAGCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCACAG
GAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTAAAATATTGGTTATGC
TTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTTTCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATT
TAATTTTCATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAAAATTCTAT
ATGCCAATAACCATCATGTGTAACTATAAATGGAAAGTTCAATTTCAAAATGTATAAACTAGGTACGTACTTGTTTTTAAATAAACTATCACACGTACAG
GATGTTGTTGGGCATTTTCTGCGGCCGCGGCTATAGAAGGAGCATATCAAATTGCAAACAACGAACTAATCTCTCTTTCAGAGCAACAACTATTGGATTG
CTCTACGCAGAACAAAGGTTGTGAGGGCGGACTAATGACTGTCGCTTACGACTTCTTACTTCAGAACAATGGCGGGGGCATTACCACAGAGACTAATTAT
CCTTATGAAGAAGCTCAAAATGTTTGCAAGACCGAACAACCAGCAGCAGTTACTATCAATGGCTACGAAGTCGTACCATCTGACGAGTCATCGTTGTTGA
AAGCTGTAGTGAATCAACCTATTTCTGTTGGTATTGCGGCTAACGATGAGTTTCATATGTACGGAAGTGGAATATATGATGGAAGTTGTAATTCTAGACT
GAATCATGCAGTGACAGTTATAGGTTATGGGACAAGTGAAGAAGATGGTACAAAATATTGGATAGTGAAGAATTCATGGGGGAGTGATTGGGGTGAGGAA
GGATATATGAGAATTGCTAGAGATGTTGGAGTTGATGGTGGCCATTGTGGCATTGCAAAGGTTGCTTCGTTCCCTACTGTTTGAATTTATATTACAAAAA
AATAGGCATTGCTATTAATATTATGTAGAAGAGTTGCCAATGCTATAGCTTGTAATTCGTGTTTCAACAATAAATTAATCAATATATGAATGAGAAGTAT
TTTTCTTTTTTAAAATGATACAGTCAAACAGTGATGGTTACTTA
The mutation and phenotype for selected feature: Solyc02g077040
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-1 (T0 ) | -1 bp/-1 bp | Morphology : "Low germination (<25%), and most don't have meristems"(Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-10 (T0 ) | -2 bp (45.1%); -5 bp (48.9%) | Morphology : Germination ~30%; Stunted growth; >80% don't have meristem (Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-11 (T0 ) | -1 bp (48.8%); -2 bp (45.1%) | Morphology : Germination ~65%; Stunted growth; ~70% don't have meristem (Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-11-3 (T1 ) | -1 bp (43.2%); -2 bp (50.2%) | |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-2 (T0 ) |
-2 bp/-2 bp
Is tDNA present: Yes
Mutation sequence: GTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTAGTAACTTCCCGCAACTTAAAAGAATTATCCATGCTTG AAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTCAAAACATTC AAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATAAGCTAGCCATCAATAAATATGCTGA TCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTATCCCAGCAAGAATCAACAGCTACAA CAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCACAGGAGTC AAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTA AAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTTTCGTCATAT TGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAG GTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : "Low germination (<25%), and most don't have meristems"(Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-6 (T0 ) | Large deletion or gene replacement? | Morphology : Low seed set; low germination(Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-7 (T0 ) | -1 bp (47.1%); -4 bp (46.9%) | Morphology : Low seed set; low germination(Phenotype Image ) |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-8 (T0 ) | -4 bp (55.8%); WT (34.6%) | |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-8-15 (T1 ) |
-4 bp/-4 bp
Is tDNA present: Yes
Mutation sequence: CTCTCTTTTAGTTTTTAACTTAGTTCCTATTTTGATATTTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCA AGAATATTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTAAACTTCCCGCAACTTAAAAGAATTAT CCATGCTTGAAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTC AAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATAAGCTAGCCATCAATAA ATATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTATCCCAGCAAGAATCAA CAGCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTC ACAGGAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATG TTCGTCTTAAAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTT TCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTAAGTATCTAAATTATAA TTTTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-8-2 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: ATCAATGGCTTCCAATTTTTTCCTCAAGAATATTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTA GTAACTTCCCGCAACTTAAAAGAATTATCCATGCTTGAAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAA AGATGATATAGAAAAAGAACACCGCTTCAAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAA CTCAACGTTATAAGCTAGCCATCAATAAATATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGAC ACTTCATTACTATCCCAGCAAGAATCAACAGCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAG CATGGACTGGAGAAAGAGAGGGAGTGTCACAGGAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATT CGTTTCACTTGAGTTGTGGTTAAGTATGTTCGTCTTAAAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTT AATATATAACTTAATATCATGTATTTTTTCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTC ATTTAGATTTTAAGTATCTAAATTATAATTTTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-8-5 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: TCTTGTACGCTAAAATTCTCAATATTACGCTTATATAAACTCTCTTAACATCGATAAGTTCTTATTCACTCAGCCTCTCT TTTAGTTTTTAACTTAGTTCCTATTTTGATATTTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCAAGAATA TTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTATAGTAACTTCCCGCAACTTAAAAGAATTATCC ATGCTTGAAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTCAA AACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATAAGCTAGCCATCAATAAAT ATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTATCCCAGCAAGAATCAACA GCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCAC AGGAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATGTT CGTCTTAAAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTTTC GTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTAAGTATCTAAATTATAATT TTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAAAATTCTATATGCCAATAACCATCATG TGTAACTATAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-9 (T0 ) | TIDE didn't work very well; -1 bp; WT | |
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-9-5 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: TCTTGTACGCTAAAATTCTCAATATTACGCTTATATAAACTCTCTTAACATCGATAAGTTCTTATTCACTCAGCCTCTCT TTTAGTTTTTAACTTAGTTCCTATTTTGATATTTCATTACCATTTACCATCAATGGCTTCCAATTTTTTCCTCAAGAATA TTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATCCCTTTATAGTAACTTCCCGCAACTTAAAAGAATTATCC ATGCTTGAAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGAAAAAGAACACCGCTTCAA AACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATAAGCTAGCCATCAATAAAT ATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTATCCCAGCAAGAATCAACA GCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAGAAAGAGAGGGAGTGTCAC AGGAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGAGTTGTGGTTAAGTATGTT CGTCTTAAAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTTAATATCATGTATTTTTTC GTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTAAGTATCTAAATTATAATT TTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAAAATTCTATATGCCAATAACCATCATG TGTAACTATAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0040 | RG-PtoR | CTG0040-1 | CGR0040-1 | CRT0040-9-6 (T1 ) |
-11 bp/-11 bp
Is tDNA present: No
Mutation sequence: CGATAAGTTCTTATTCACTCAGCCTCTCTTTTAGTTTTTAACTTAGTTCCTATTTTGATATTTCATTACCATTTACCATC AATGGCTTCCAATTTTTTCCTCAAGAATATTACTGTTGTTCTTCTTCTTTTTTCCATTCTTAGCCTATATAACTTCCCGC AACTTAAAAGAATTATCCATGCTTGAAAGGCATGAAAATTGGATGGTTCATCATGGACGTGTATACAAAGATGATATAGA AAAAGAACACCGCTTCAAAACATTCAAGGAAAACGTTGAGTTCATCGAATCTTTCAACAAGAACGGAACTCAACGTTATA AGCTAGCCATCAATAAATATGCTGATCTGACCACTGAGGAATTCACAACATCATTTATGGGGCTCGACACTTCATTACTA TCCCAGCAAGAATCAACAGCTACAACAACGTCCTTTAAATATGATAGTGTGACTGAAGTTCCAAATAGCATGGACTGGAG AAAGAGAGGGAGTGTCACAGGAGTCAAGGATCAAGGTGTATGTGGTAAGACCAGTTCAAATTCTTATTCGTTTCACTTGA GTTGTGGTTAAGTATGTTCGTCTTAAAATATTGGTTATGCTTGTCGAGAATCAAACTATATGAATTTTAATATATAACTT AATATCATGTATTTTTTCGTCATATTGATACGAGAAGATCAATTCCTACTTATTGTTATTTAATTTTCATTTAGATTTTA AGTATCTAAATTATAATTTTAAAAGGTGACCACTATTTTTGAATAGACAGAGTATATATGTGTGCTCTTGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
