Plant Crispr Database

Feature Information

Feature ID: Solyc02g094060
Feature Type: gene
Name: Transporter gene
Description: Voltage-gated chloride channel
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc02g094060

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
CRT0138	RG-PtoR	CTG0138-2	CGR0138-2	CRT0138-7 (T0 )	-5 bp (47.9%)/ -2 bp (45.9%)
CRT0138	RG-PtoR	CTG0138-2	CGR0138-2	CRT0138-7-11 (T1 )	-2 bp/-2 bp Is tDNA present: No Mutation sequence: ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 2417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 50 Wildtype 2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 2467 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 100 Wildtype 2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 2517 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 150 Wildtype 2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 2567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 200 Wildtype 2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 2617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 250 Wildtype 2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 2667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 300 Wildtype 2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 2717 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 350 Wildtype 2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 2767 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 400 Wildtype 2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC 2817 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC 448 gRNA GATGAGGGTGGCGACACCTC Wildtype 2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 2867 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 498 Wildtype 2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 2917 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 548 Wildtype 2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 2967 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 598 Wildtype 2968 ACAGATAAGGTTCAGTATGAGT 2989 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 599 ACAGATAAGGTTCAGTATGAGT 620	Morphology : Retarded growth; Germination: 75%
CRT0138	RG-PtoR	CTG0138-2	CGR0138-2	CRT0138-7-7 (T1 )	-2 bp/-2 bp Is tDNA present: No Mutation sequence: ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 2417 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT 50 Wildtype 2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 2467 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT 100 Wildtype 2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 2517 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG 150 Wildtype 2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 2567 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT 200 Wildtype 2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 2617 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT 250 Wildtype 2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 2667 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA 300 Wildtype 2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 2717 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA 350 Wildtype 2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 2767 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG 400 Wildtype 2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC 2817 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC 448 gRNA GATGAGGGTGGCGACACCTC Wildtype 2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 2867 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT 498 Wildtype 2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 2917 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA 548 Wildtype 2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 2967 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA 598 Wildtype 2968 ACAGATAAGGTTCAGTATGAGT 2989 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 599 ACAGATAAGGTTCAGTATGAGT 620	Morphology : Retarded growth; Germination: 90%

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0138

RG-PtoR

CTG0138-2

CGR0138-2

CRT0138-7 (T0 )

-5 bp (47.9%)/ -2 bp (45.9%)

CRT0138

RG-PtoR

CTG0138-2

CGR0138-2

CRT0138-7-11 (T1 )

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT
TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC
AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG
GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG
TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG
GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC
AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG
ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT   2417
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT     50

Wildtype        2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT   2467
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT    100

Wildtype        2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG   2517
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG    150

Wildtype        2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT   2567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT    200

Wildtype        2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT   2617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT    250

Wildtype        2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA   2667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA    300

Wildtype        2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA   2717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA    350

Wildtype        2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG   2767
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG    400

Wildtype        2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC   2817
                     ||||||||||||||||||  ||||||||||||||||||||||||||||||
MutationRev      401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC    448
gRNA                      GATGAGGGTGGCGACACCTC

Wildtype        2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT   2867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT    498

Wildtype        2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA   2917
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA    548

Wildtype        2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA   2967
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA    598

Wildtype        2968 ACAGATAAGGTTCAGTATGAGT   2989
                     ||||||||||||||||||||||
MutationRev      599 ACAGATAAGGTTCAGTATGAGT    620

Morphology : Retarded growth; Germination: 75%

CRT0138

RG-PtoR

CTG0138-2

CGR0138-2

CRT0138-7-7 (T1 )

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

ACTCATACTGAACCTTATCTGTTTTGGCAGAGATAATGAAATAGATTTGTTGTATTTACATGTCATTATGTGTCTCTGAT
TTAGGATCAATGAAAATGATCTCTTCAAGCATGATTGGAGAAAAAGATCAAGAGTTCAAACATTACAGTATGTCTTCATC
AAATGGACACTCGCATTCCTGGTCGGCCTTTTTACCGGAGGTCGCCACCCTCATCAGTCTCTCAATCGAAAATATCTCTG
GTTACAAACTCCGCGCTACTGTCAACTATATTGATGACAAAAGGTTAATAAGTAGTAAAATTTATTTCTCGTCTCGTTTG
TTCTAATGACTTACAATTATGATAGTCTGACTTAATTAAAACAATTATGTAGGTACTTTATGGGATTTGCATTTTTTGCG
GGTGCTAACTTTCTGTTGACATTGCTAGCTGCCTTTATCACTGTTTATTTTGCACCTACGGCTGCCGGGCCTGGAGTTCC
AGAAATTAAAGCCTATCTCAATGGGGTAGACACTCCCAATATGTTCGGGGCAACTACACTCTTTGTTAAGGTTAATGCTG
ATTTTTATCAACAGTGTTCTCATTTACCCTCTAGCATGACTTGAGACCACAACTTATCCA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        2368 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT   2417
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 TGGATAAGTTGTGGTCTCAAGTCATGCTAGAGGGTAAATGAGAACACTGT     50

Wildtype        2418 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT   2467
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TGATAAAAATCAGCATTAACCTTAACAAAGAGTGTAGTTGCCCCGAACAT    100

Wildtype        2468 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG   2517
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATTGGGAGTGTCTACCCCATTGAGATAGGCTTTAATTTCTGGAACTCCAG    150

Wildtype        2518 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT   2567
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GCCCGGCAGCCGTAGGTGCAAAATAAACAGTGATAAAGGCAGCTAGCAAT    200

Wildtype        2568 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT   2617
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 GTCAACAGAAAGTTAGCACCCGCAAAAAATGCAAATCCCATAAAGTACCT    250

Wildtype        2618 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA   2667
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ACATAATTGTTTTAATTAAGTCAGACTATCATAATTGTAAGTCATTAGAA    300

Wildtype        2668 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA   2717
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 CAAACGAGACGAGAAATAAATTTTACTACTTATTAACCTTTTGTCATCAA    350

Wildtype        2718 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG   2767
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 TATAGTTGACAGTAGCGCGGAGTTTGTAACCAGAGATATTTTCGATTGAG    400

Wildtype        2768 AGACTGATGAGGGTGGCGACACCTCCGGTAAAAAGGCCGACCAGGAATGC   2817
                     ||||||||||||||||||  ||||||||||||||||||||||||||||||
MutationRev      401 AGACTGATGAGGGTGGCG--ACCTCCGGTAAAAAGGCCGACCAGGAATGC    448
gRNA                      GATGAGGGTGGCGACACCTC

Wildtype        2818 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT   2867
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      449 GAGTGTCCATTTGATGAAGACATACTGTAATGTTTGAACTCTTGATCTTT    498

Wildtype        2868 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA   2917
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      499 TTCTCCAATCATGCTTGAAGAGATCATTTTCATTGATCCTAAATCAGAGA    548

Wildtype        2918 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA   2967
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      549 CACATAATGACATGTAAATACAACAAATCTATTTCATTATCTCTGCCAAA    598

Wildtype        2968 ACAGATAAGGTTCAGTATGAGT   2989
                     ||||||||||||||||||||||
MutationRev      599 ACAGATAAGGTTCAGTATGAGT    620

Morphology : Retarded growth; Germination: 90%

Feature Information

Sequence & Structure:

Name: Solyc02g094060 (Exon Intron) Length: 4984

The mutation and phenotype for selected feature: Solyc02g094060

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc02g094060 (Exon Intron)
Length: 4984