Name: Solyc03g093560
(Exon Intron)
Length: 735
TTATATCATAACAAGCTGAGATAATGGTGACAATGGTGGCACCTCAAAAATACCCTTTTCATCCCAAATCGTCGACCAACTTGACGGTGTTAATGGAGCCGCCGCCGTTGGGAGTACTCTTTCTTCTTCTATTTTCCTTTCCTTCTTGATTACTAATTCCTCTGTTTCTCCTCTCACCCTTTTACTGCCTGACGACTTCA
TCTCTGTTTTAGTCTCATCATTTTGTTGCTTAAAGTTTGCAACTTCGAGTGGGAAATTCAATATTGCTTTGCTTCCTCTAAGCTTAAACGCTGCCCTGTC
ATATGCCTTTGCCGCATCCACAGCAGTATCAAATGTTCCTAACCAAACTCGAGTCCCCTTTCTATTTGGGTCACGAATCTCCGCTGCAAACTTCCCCCAT
GGCCTCTGTCTAACTCCCCTGTAATGCTTCTTCTCAATCTCAACGTTCTCTACAACAACAACAGGCTTCGCCGGTATTGCTACGTTCAGAGATGGCTTCC
GTTCTCTCAAGTTACTCTGTTTCGGAGAATTTGAATGGATAACATGTCGTTTTGCCTCAAATTCGAAAAAACCATCATACTCTGTTTTTGAAGTAGCATT
AAAGAAGCTTGCGATGTCATCAAGATTTGAAGTTTGAGTTGTTTCAAAAGAGAAACAAGTCTGGTCCAGAGATTCATCAAAAAGATGTTGCCTAATCAAA
TCAAGTGAAGTACAAGTCTCTTGTGGAGAACCCAT
The mutation and phenotype for selected feature: Solyc03g093560
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-1 (T0 ) | -7 bp (50.4%); WT (43.1%) | |
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-1-14 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: ATGGTTTTTTCGAATTTGAGGCAAAACGACATGTTATCCATTCAAATTCTCCGAAACAGAGTAACTTGAGAGAACGGAAG CCATCTCTGAACGTAGCAATACCGGCGAAGCCTGTTGTTGTTGTAGAGAACGTTGAGATTGAGAAGAAGCATTACAGGGG AGTTAGACAGAGGCCATGGGGGAAGTTTGCAGCGGAGATTCGTGACCCAAATAGAAAGGGGACTCG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-1-16 (T1 ) |
-7 bp /-7 bp
Is tDNA present: Yes
Mutation sequence: ACTTCAAATCTTGATGACATCGCAAGCTTCTTTAATGCTACTTCAAAAACAGAGTATGATGGTTTTTTCGAATTTGAGGC AAAACGACATGTTATCCATTCAAATTCTCCGAAACAGAGTAACTTGAGAGAACGGAAGCCATCTTAGCAATACCGGCGAA GCCTGTTGTTGTTGTAGAGAACGTTGAGATTGAGAAGAAGCATTACAGGGGAGTTAGACAGAGGCCATGGGGGAAGTTTG CAGCGGAGATTCGTGACCCAAATAGAAAGGGGACTCGAGTTTGGTTAGGAACATTTGATACTGCTGTGGATGCGGCAAAG GCATATGACAGGGCAGCGTTTAAGCTTAGAGGAAGCAAAGCAATATTGAATTTCCCACTCGAAGTTGCAAACTTTAAGCA ACAAAATGATGAGACTAAAACAGAGATGAAGTCGTCAGGCAGTAAAAGGGTGAGAGGAGAAACAGAGGAATTAGTAATCA AGAAGGAAAGGAAAATAGAAGAAGAAAGAGTACTCCCAACGGCGGCGGCTCCATTAACACCGTCAAGTTGGTCGACGATT TGGGATGAAAAGGGTATTTTTGAGGTGCCACCATTGTCACCATTATCTCAGCTTGTTATGATATAATTTTGGATTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-2 (T0 ) | -2 bp (97.7%); -1 bp (1.1%) | |
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-3 (T0 ) | -2 bp (97.7%); -1 bp (0.8%) | |
| CRT0103 | RG-PtoR | CTG0103-1 | CGR0103-1 | CRT0103-4 (T0 ) | WT (91.2%); -1 bp (3.7%); -2 bp (3.5%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
