Name: Solyc03g096190
(Exon Intron)
Length: 3213
ATGATTCTCCCAAAGAATTCTCACTTTTATGCTATGAAGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTATGTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTTTCAAGATCATGGA
CTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCATGTGGTAACCGTCATTGCCGAGTTACAGTTTTAAATCTTACTTCTCTGAAATTG
AGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGCTTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCT
ACTTACATAAGTTAAACGTTTTACGCCTTGATAATAACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTTGGTCT
TTCGTATAATATGATGGTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTTTGACAGGAGGAATT
CCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATAATTTGTTAGGGAAAATACCTGATGAATTATGTCAATTACTCAACT
TAAAGTACTTTGTAGTGAATGAAAACAACTTGAGTAGTACCCTGCCTCCTTGTCTTTTCAATCTTTCCTCTATCGTGGCCATCGATGTTGGAACAAACCA
TTTAGAAGGACAATTGCCTCCATTGCTTGGTATTACTCTTCCTAAGTTAGAGTTCCTTAGCATTTATAGAAACAATGTAACTGGAAATATTCCGGGGACA
TTGTCAAATGCTACAAATCTTCAGTCTCTTATTGCTGGTAGAAATGGACTCACAGGAAAAGTACCACCCCTTGGAAATTTGCTGAAAATGAGGAGATTTT
TAGTTGCTTTCAATGATCTTGGCAAGGAGGAGGCGGATGACCTTAGTTTTCTCTCCACATTGGTGAATGCCACCAACTTGGAGCTTGTGGAGCTCAATAC
AAATAATTTTGGTGGAGTCTTACCTGCATCTGTTAGTAATCTTTCGACTGAGCTTATAGAGCTTTCGTTGTCTTATAATCAAGTTTCTGGAGAAATCCCA
AGAGGGATATCCAATCTCAAGAAGCTTCAGGCTTTCTTTGTAGCTTACAACAGATTTATCGGTGAAATTCCTTCTGAAATTGGTGATCTTATGTATTTGC
AAGAGCTGGCTTTACTTGGAAATCAGTTCTCTGGCCAAATTCCAATCTCATTGGGAAATTTAGCTTCTTTAACCAAACTCACTTTAAGAGAAAACAATCT
CCAGGGGAGAATTCCTTCCAGTTTAGGCAAATGCGACAAGTTGGAACTGTTGGATCTTGGTTCAAATAACCTTAGTGGTTTCATACCATCAGAAATTCTT
GAACTCTCATCATTGTCGGAGGGCGTGGACCTATCTCAGAACCACTTAACTGGTTTCCTTCCGATGGGAATTGGGAAGTTGAGAAATCTAGGCTACTTAA
ACCTGTCTTATAACAAGTTGCAGGGCCAGATTCCTACCACTATAGGTACTTGTGTGAAACTTGAAGCACTAGACTTAAACAACAACAACTTTCAGGGTAG
TATTCCTTCAACCATGAATAATTTGCGAGGCCTGGAATTTTTAGTCCTTTCTCACAACAATCTGTCAGGTGGAATCCCGGGATTCTTGAAGGACTTCAAA
TTTCTTCAGATTTTAAATCTGTCTAGCAATAATTTGGAAGGTGCTGTCCCAACTGGAGGTATCTTTAGTAATGCTACTGCGGTTTCCATCATAGGAAATA
AAAATCTCTGTGGGGGTGTACCGGAGTTGGACCTTCCGGTTTGCATTGTAGGAGTAAAGAAAGAAAGGAAATCAGGTTTTCCCTTGAAAAAAGTAATTCC
TGTAGTTAGTGGGCTTATAGGATTGACATTGATAGTTTGTTTTCTTGGCATACGACAGTTTAGTAGATTGAGAAAAACAACACCTACAGATATACCTGAA
AACTCAACCTTAAGAATATCTTATCAGTGTCTACTTAGGGAAACTGATAGATTTTCTGCATCAAATTTGCTTGGCATGGGTGCTTTTGGGTCTGTATATA
AAGGAATTTCTGAACATGATGGTACTGTTTTTGCTGTTAAGGTACTGGACCTTTCACACCATGCAGCTTCCAGGAGTTTTTTAGCTGAATGTGAGGTTCT
AAAGAACATCAGACATCGTAATCTTGTAAAGGTTCTAAGTGCATGCTCAGGTATTGATTATGAAGGTAATGAGTTCAAAGCCATAGTTTATGAATACATG
GATAAGGGGAATCTACAGGACTGGTTGCATTTCACTCCTCAGGAGAACTCTGAGCCACAGGAGGAACACAAGAAGCTAGGGTTTATTCAAAGATTAAACA
TTGCGATTGATGTTGCCTGTGCTCTGGATTATCTCCATAATGATTGCCAACCCCCTATAATTCACCGCGATCTGAAGCCAAGCAATATTCTTCTAGATGA
GAACATGACTGCACATGTTGGTGATTTTGGTTTGGCAAGATTTGTTCCACCAGAAATTCCGAACTCATCAGAAAATTCAAAAAGTCTTACAGGTGTAGGG
GGGACAATTGGTTATACACCTCCAGGTAATAATAAATCAAGGTTTTTGCTGTCATACATATGTCTTTTGATAAGATTATCTCTAATTCTGTAGTTTTTCA
TGCTGACAGAATTAGGTATGGGAAGCGACGCCTCAACCTATGGAGATGGATACAGCTTTGGCATACTGCTCTTGGAAATGTTTACCGGGAGGAAACCAAC
TGATGAAATGTTCAAAGATAATTTAAACCTTCATAACTACGCGAATGCTGCTTTGCCAGACAGAGTGATGCATATTACAGATCCAATTCTTCTACAGGAA
CGAGATGAACTTGAAATGGAATACAAACTTCATGACAACACAAGCAGTGCTGGCGACATATTTCTGTCATTCTTAATCAACGTGATTCAAATCGGAGTTT
CCTGCTCCGCTGAATCTCCAAAAGAAAGAAAGCGGATCAGTGATGTCGTTAGAGAACTCAATTCATTAAGGAAGTTGTTTTTGGAGCAAGCATACCGGAA
GAAAAAGTTATAA
The mutation and phenotype for selected feature: Solyc03g096190
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0032 | RG-PtoR | CTG0032-1 | CGR0032-1 | CRT0032-5 (T0 ) | -8 bp (49.6%); +1 bp (46.5%) | Morphology : (Phenotype Image ) Immunity response: Lost response to csp22 |
| CRT0032 | RG-PtoR | CTG0032-1 | CGR0032-1 | CRT0032-5-5 (T1 ) | -8 bp (48.4%); +1 bp (47.8%) | |
| CRT0032 | RG-PtoR | CTG0032-1 | CGR0032-1 | CRT0032-7 (T0 ) | +1 bp (48.7%); -5 bp (47.7%) | Morphology : (Phenotype Image ) Immunity response: Lost response to csp22 |
| CRT0032 | RG-PtoR | CTG0032-1 | CGR0032-1 | CRT0032-7-20 (T1 ) |
-5 bp/-5 bp
Is tDNA present: No
Mutation sequence: ATGCAGGTAAGACTCCACCAAAATTATTTGTATTGAGCTCCACAAGCTCCAAGTTGGTGGCATTCACCAATGTGGAGAGA AAACTAAGGTCATCCGCCTCCTCCTTGCCAAGATCATTGAAAGCAACTAAAAATCTCCTCATTTTCAGCAAATTTCCAAG GGGTGGTACTTTTCCTGTGAGTCCATTTCTACCAGCAATAAGAGACTGAAGATTTGTAGCATTTGACCCCCGGAATATTT CCAGTTACATTGTTTCTATAAATGCTAAGGAACTCTAACTTAGGAAGAGTAATACCAAGCAATGGAGGCAATTGTCCTTC TAAATGGTTTGTTCCAACATCGATGGCCACGATAGAGGAAAGATTGAAAAGACAAGGAGGCAGGGTACTACTCAAGTTGT TTTCATTCACTACAAAGTACTTTAAGTTGAGTAATTGACATAATTCATCAGGTATTTTCCCTAACAAATTATTCTTGCTT GCAGAAAAGGTATCAAGTAAAGAAAGATTACCAAACGAAGGCGGAATTCCTCCTGTCAAAGAGTTAGAAACAAGAGAAAG TTGTTTGAGTCTCAACAATGTGCCTAATTCTGCTGGAATTTCGCCTACCATCATATTATACGAAAGACCAACAGAAACAA GATTGAAGCAACCAGAAATGTTTGAAGGAATATGACCAGTGAACGAGTTATTATCAAGGCGTAAAACGTTTAACTTATGT AAGTAGCCAATCTCTGATGGGATTTCACCTGAAAAACTATTATTTTGAAGCTTAAGGACATTAAGAAAGCTCAGGTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0032 | RG-PtoR | CTG0032-1 | CGR0032-1 | CRT0032-7-7 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: AGGTAAGACTCCACCAAAATTATTTGTATTGAGCTCCACAAGCTCCAAGTTGGTGGCATTCACCAATGTGGAGAGAAAAC TAAGGTCATCCGCCTCCTCCTTGCCAAGATCATTGAAAGCAACTAAAAATCTCCTCATTTTCAGCAAATTTCCAAGGGGT GGTACTTTTCCTGTGAGTCCATTTCTACCAGCAATAAGAGACTGAAGATTTGTAGCATTTGACAATGTTCCCCGGAATAT TTCCAGTTACATTGTTTCTATAAATGCTAAGGAACTCTAACTTAGGAAGAGTAATACCAAGCAATGGAGGCAATTGTCCT TCTAAATGGTTTGTTCCAACATCGATGGCCACGATAGAGGAAAGATTGAAAAGACAAGGAGGCAGGGTACTACTCAAGTT GTTTTCATTCACTACAAAGTACTTTAAGTTGAGTAATTGACATAATTCATCAGGTATTTTCCCTAACAAATTATTCTTGC TTGCAGAAAAGGTATCAAGTAAAGAAAGATTACCAAACGAAGGCGGAATTCCTCCTGTCAAAGAGTTAGAAACAAGAGAA AGTTGTTTGAGTCTCAACAATGTGCCTAATTCTGCTGGAATTTCGCCTACCATCATATTATACGAAAGACCAACAGAAAC AAGATTGAAGCAACCAGAAATGTTTGAAGGAATATGACCAGTGAACGAGTTATTATCAAGGCGTAAAACGTTTAACTTAT GTAAGTAGCCAATCTCTGATGGGATTTCACCTGAAAAACTATTATTTTGAAGCTTAAGGACATTAAGAAAGCTCAGGTTA CCAATGCTTGGTGACAGAGAACCCCTCAATTTCAGAGAAGTAAGATTTAAAACTGTAACTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0032 | RG-PtoR | CTG0032-2 | CGR0032-2 | CRT0032-4 (T0 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: ATTCTCACTTTTATGCTATGAAGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTAT GTTCTTTGCTCATCAAAAATTCAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCAT TGATGACCCTTTCAAGATCATGGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCCATGTGGTAAC CGTCATTGCCGAGTTACAGTTTTAAATCTTACTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAG CTTTCTTAATGTCCTTAAGCTTCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCTACTTACATAAGTTAA ACGTTTTACGCCTTGATAATAACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTT GGTCTTTCGTATAATATGATGGTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGT TTCTAACTCTTTGACAGGAGGAATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATA ATTTGTTAGGGAAAATACCTGATGAATTATGTCAATTACTCAACTTAAAGTACTTTGTAGTGAATGAAAACAACTTGAGT AGTACCCTGCCTCCTTGTCTTTTCAATCTTTCCTCTATCGTGGCCATCGATGTTGGAACAAACCATTTAGAAGGACAATT GCCTCCATTGCTTGGTATTACTCTTCCTAAGTTAGAGTTCCTTAGCATTTATAGAAACAATGTAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : (Phenotype Image ) Immunity response: Lost response to csp22 |
| CRT0032 | RG-PtoR | CTG0032-2 | CGR0032-2 | CRT0032-4-3 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: AGAAGAAGTATACTGTTGTACTATCTTTACTGACCTTTTTCTCTTTGATACTACCTTATGTTCTTTGCTCATCAAAAATT CAGGTGAAAGGGAATGAAACAGACAAAATGTCACTGCTAGCATTCAAGAATATGATCATTGATGACCCTTTCAAGATCAT GGACTCCTGGAATGAAACTATACATTTCTGTGACTGGCCTGGGGTCTCCATGTGGTAACCGTCATTGCCGAGTTACAGTT TTAAATCTTACTTCTCTGAAATTGAGGGGTTCTCTGTCACCAAGCATTGGTAACCTGAGCTTTCTTAATGTCCTTAAGCT TCAAAATAATAGTTTTTCAGGTGAAATCCCATCAGAGATTGGCTACTTACATAAGTTAAACGTTTTACGCCTTGATAATA ACTCGTTCACTGGTCATATTCCTTCAAACATTTCTGGTTGCTTCAATCTTGTTTCTGTTGGTCTTTCGTATAATATGATG GTAGGCGAAATTCCAGCAGAATTAGGCACATTGTTGAGACTCAAACAACTTTCTCTTGTTTCTAACTCTTTGACAGGAGG AATTCCGCCTTCGTTTGGTAATCTTTCTTTACTTGATACCTTTTCTGCAAGCAAGAATA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
