Plant Crispr Database

Feature Information

Feature ID: Solyc03g111800
Feature Type: gene
Name: SOBIR-like
Description: Suppressor Of BIR1-1-like, RLK
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc03g111800

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
CRT0020	RG-PtoR	CTG0020-2	CGR0020-2	CRT0020-2 (T0 )	+1 bp/+1 bp Is tDNA present: Yes Mutation sequence: GTACCTCATCCACCTCCCTATTCCACCACAATACTGTCGGAAAACACCAATCCGATGACCTTCACAGCCTCATATATACA CCTTTCTTTTTTGTCTTTTTTCACTGTCATCCTTCTTGTTCAAGCAAAACTAAACCTTTACTCACCTGATCACAGTGCTC TTTTGCTTGTCCAAAAAGGCTTAGGCATCCCTGCTCAGCGCAATGTTCTTGAGAACCCATGCAACTCTGTTGGAATATCA TGCGAAAAACGACTCATAAACAATTCAAATGTGCTAAGAGTCACAAGGGTTGTCTTCAAATCCTACGGGTTGAAGGGTAC TCTGTCTCCTGCCNATTGGGAGGCTTTCTGAGCTCAAAGAGCTGTCCCTCCAAAACAACAAACTCTTTGACAGAATACCA ACTGAAATAGTTGACTCCAGAAAATTGGAAATCTTGAACCTTCAAAACAACCAATTTTCTGGCAAAGTCCCACCTGAATT ATCATCTCTAGTCCGACTTCGTATCCTTGACCTTGGCTCTAATGAGTTATCTGGGAACCTGAACTTCTTGAAATACTTTC CTAACCTTGAAAAACTCTCCCTTGCTGAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1692 ATCAGCAAGGGAGAGTTTTTCAAGGTTAGGAAAGTATTTCAAGAAGTTCA 1741 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATCAGCAAGGGAGAGTTTTTCAAGGTTAGGAAAGTATTTCAAGAAGTTCA 50 Wildtype 1742 GGTTCCCAGATAACTCATTAGAGCCAAGGTCAAGGATACGAAGTCGGACT 1791 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GGTTCCCAGATAACTCATTAGAGCCAAGGTCAAGGATACGAAGTCGGACT 100 Wildtype 1792 AGAGATGATAATTCAGGTGGGACTTTGCCAGAAAATTGGTTGTTTTGAAG 1841 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AGAGATGATAATTCAGGTGGGACTTTGCCAGAAAATTGGTTGTTTTGAAG 150 Wildtype 1842 GTTCAAGATTTCCAATTTTCTGGAGTCAACTATTTCAGTTGGTATTCTGT 1891 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 GTTCAAGATTTCCAATTTTCTGGAGTCAACTATTTCAGTTGGTATTCTGT 200 Wildtype 1892 CAAAGAGTTTGTTGTTTTGGAGGGACAGCTCTTTGAGCTCAGAAAGCCTC 1941 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 CAAAGAGTTTGTTGTTTTGGAGGGACAGCTCTTTGAGCTCAGAAAGCCTC 250 Wildtype 1942 CCAAT-GGCAGGAGACAGAGTACCCTTCAACCCGTAGGATTTGAAGACAA 1990 \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 CCAATNGGCAGGAGACAGAGTACCCTTCAACCCGTAGGATTTGAAGACAA 300 gRNA AAT-GGCAGGAGACAGAGTAC Wildtype 1991 CCCTTGTGACTCTTAGCACATTTGAATTGTTTATGAGTCGTTTTTCGCAT 2040 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CCCTTGTGACTCTTAGCACATTTGAATTGTTTATGAGTCGTTTTTCGCAT 350 Wildtype 2041 GATATTCCAACAGAGTTGCATGGGTTCTCAAGAACATTGCGCTGAGCAGG 2090 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GATATTCCAACAGAGTTGCATGGGTTCTCAAGAACATTGCGCTGAGCAGG 400 Wildtype 2091 GATGCCTAAGCCTTTTTGGACAAGCAAAAGAGCACTGTGATCAGGTGAGT 2140 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 GATGCCTAAGCCTTTTTGGACAAGCAAAAGAGCACTGTGATCAGGTGAGT 450 Wildtype 2141 AAAGGTTTAGTTTTGCTTGAACAAGAAGGATGACAGTGAAAAAAGACAAA 2190 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 AAAGGTTTAGTTTTGCTTGAACAAGAAGGATGACAGTGAAAAAAGACAAA 500 Wildtype 2191 AAAGAAAGGTGTATATATGAGGCTGTGAAGGTCATCGGATTGGTGTTTTC 2240 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 AAAGAAAGGTGTATATATGAGGCTGTGAAGGTCATCGGATTGGTGTTTTC 550 Wildtype 2241 CGACAGTATTGTGGTGGAATAGGGAGGTGGATGAGGTAC 2279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 CGACAGTATTGTGGTGGAATAGGGAGGTGGATGAGGTAC 589	Morphology : Abiotic stress; late and low fruit set

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0020

RG-PtoR

CTG0020-2

CGR0020-2

CRT0020-2 (T0 )

+1 bp/+1 bp

Is tDNA present: Yes

Mutation sequence:

GTACCTCATCCACCTCCCTATTCCACCACAATACTGTCGGAAAACACCAATCCGATGACCTTCACAGCCTCATATATACA
CCTTTCTTTTTTGTCTTTTTTCACTGTCATCCTTCTTGTTCAAGCAAAACTAAACCTTTACTCACCTGATCACAGTGCTC
TTTTGCTTGTCCAAAAAGGCTTAGGCATCCCTGCTCAGCGCAATGTTCTTGAGAACCCATGCAACTCTGTTGGAATATCA
TGCGAAAAACGACTCATAAACAATTCAAATGTGCTAAGAGTCACAAGGGTTGTCTTCAAATCCTACGGGTTGAAGGGTAC
TCTGTCTCCTGCCNATTGGGAGGCTTTCTGAGCTCAAAGAGCTGTCCCTCCAAAACAACAAACTCTTTGACAGAATACCA
ACTGAAATAGTTGACTCCAGAAAATTGGAAATCTTGAACCTTCAAAACAACCAATTTTCTGGCAAAGTCCCACCTGAATT
ATCATCTCTAGTCCGACTTCGTATCCTTGACCTTGGCTCTAATGAGTTATCTGGGAACCTGAACTTCTTGAAATACTTTC
CTAACCTTGAAAAACTCTCCCTTGCTGAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1692 ATCAGCAAGGGAGAGTTTTTCAAGGTTAGGAAAGTATTTCAAGAAGTTCA   1741
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ATCAGCAAGGGAGAGTTTTTCAAGGTTAGGAAAGTATTTCAAGAAGTTCA     50

Wildtype        1742 GGTTCCCAGATAACTCATTAGAGCCAAGGTCAAGGATACGAAGTCGGACT   1791
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GGTTCCCAGATAACTCATTAGAGCCAAGGTCAAGGATACGAAGTCGGACT    100

Wildtype        1792 AGAGATGATAATTCAGGTGGGACTTTGCCAGAAAATTGGTTGTTTTGAAG   1841
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AGAGATGATAATTCAGGTGGGACTTTGCCAGAAAATTGGTTGTTTTGAAG    150

Wildtype        1842 GTTCAAGATTTCCAATTTTCTGGAGTCAACTATTTCAGTTGGTATTCTGT   1891
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 GTTCAAGATTTCCAATTTTCTGGAGTCAACTATTTCAGTTGGTATTCTGT    200

Wildtype        1892 CAAAGAGTTTGTTGTTTTGGAGGGACAGCTCTTTGAGCTCAGAAAGCCTC   1941
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 CAAAGAGTTTGTTGTTTTGGAGGGACAGCTCTTTGAGCTCAGAAAGCCTC    250

Wildtype        1942 CCAAT-GGCAGGAGACAGAGTACCCTTCAACCCGTAGGATTTGAAGACAA   1990
                     ||||| ||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 CCAATNGGCAGGAGACAGAGTACCCTTCAACCCGTAGGATTTGAAGACAA    300
gRNA                   AAT-GGCAGGAGACAGAGTAC

Wildtype        1991 CCCTTGTGACTCTTAGCACATTTGAATTGTTTATGAGTCGTTTTTCGCAT   2040
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 CCCTTGTGACTCTTAGCACATTTGAATTGTTTATGAGTCGTTTTTCGCAT    350

Wildtype        2041 GATATTCCAACAGAGTTGCATGGGTTCTCAAGAACATTGCGCTGAGCAGG   2090
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 GATATTCCAACAGAGTTGCATGGGTTCTCAAGAACATTGCGCTGAGCAGG    400

Wildtype        2091 GATGCCTAAGCCTTTTTGGACAAGCAAAAGAGCACTGTGATCAGGTGAGT   2140
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 GATGCCTAAGCCTTTTTGGACAAGCAAAAGAGCACTGTGATCAGGTGAGT    450

Wildtype        2141 AAAGGTTTAGTTTTGCTTGAACAAGAAGGATGACAGTGAAAAAAGACAAA   2190
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 AAAGGTTTAGTTTTGCTTGAACAAGAAGGATGACAGTGAAAAAAGACAAA    500

Wildtype        2191 AAAGAAAGGTGTATATATGAGGCTGTGAAGGTCATCGGATTGGTGTTTTC   2240
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 AAAGAAAGGTGTATATATGAGGCTGTGAAGGTCATCGGATTGGTGTTTTC    550

Wildtype        2241 CGACAGTATTGTGGTGGAATAGGGAGGTGGATGAGGTAC   2279
                     |||||||||||||||||||||||||||||||||||||||
MutationRev      551 CGACAGTATTGTGGTGGAATAGGGAGGTGGATGAGGTAC    589

Morphology : Abiotic stress; late and low fruit set

Feature Information

Sequence & Structure:

Name: Solyc03g111800 (Exon Intron) Length: 2320

The mutation and phenotype for selected feature: Solyc03g111800

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc03g111800 (Exon Intron)
Length: 2320