Plant Crispr Database

Feature Information

Feature ID: Solyc04g005070
Feature Type: gene
Name: Transporter gene
Description: Peptide transporter
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc04g005070

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
CRT0138	RG-PtoR	CTG0138-7	CGR0138-7	CRT0138-2 (T0 )	-1 bp (49.5%) / wt (45.1 %)
CRT0138	RG-PtoR	CTG0138-7	CGR0138-7	CRT0138-2-4 (T1 )	-2 bp/-2 bp Is tDNA present: No Mutation sequence: CTGACACTTTTATCACAAGTTCTTATACTTGCTCACTTACTCATCAGTGAGGAATTCAAGATTATACGGCTTATTCATGT TTTTCATCTTTAGAACTTGTTTAGTTGTAGAGGCTTATTCCTTTTTATCTCAACAGGGAACGTGTATTCTTTGGCTCACC ACAATAATGCCACAACTCCGGCCTACCTTGCGGTCAGCATCAACATGTTTGTGATGGAACAACAGCAGCCCAACTTGCTA TACTTTTGTGTTCTTTGGGGCTTATGTCCATTGGTACTGGTTTTAACAGACCTTGTTCCATAGCATTTGGTGCTGACCAA TTGGAGAACAAAGAAAATCCAGATAACGAGAGGCTTATAGATAGCTATTTCAACTGGTATTATGCCAGTGTAGGAGTTTC ACTTGTTCTTGCAGTTACTGTAATTGTTTATATCCAAGACCATTTTGGTTGGCAAATTGGGTTTGGTGTCCCTGCTTTGC TCATGCTTATATCTGTTTCCATTTTCTTGATCGGATCTCCTCTTTATATCAAAGCAAAAGCTAGAGGGAGTTTGTTCACT GGATTGTTTCAAGTAGCAGTGGCAGCCTTCAGAAAAAGACATATCAATGTTGAGTCAAATTATATTGCTGACTGCTACTA TAAAGAACCTGAATCGAACCTGCTCGAACCATCTGCCGACTTCAGGTATAGGTGCCTCCATCTTTTCTTCAATGGTATAG ACATCTTCAACATCCATTCATGCACTTAGTATCTTGCAATACTAATTTGTTATACTGTATTACATGATGAACTCGTGTGA T Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 1633 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA 1682 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA 50 Wildtype 1683 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG 1732 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG 100 Wildtype 1733 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT 1782 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT 150 Wildtype 1783 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA 1832 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA 200 Wildtype 1833 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA 1882 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA 250 Wildtype 1883 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA 1932 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA 300 Wildtype 1933 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC 1982 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC 350 Wildtype 1983 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG 2032 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG 400 Wildtype 2033 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC 2082 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC 450 Wildtype 2083 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT 2132 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT 500 Wildtype 2133 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA 2182 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA 550 Wildtype 2183 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT 2232 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT 600 Wildtype 2233 GATGCTGACCGCAAGGTAAAGGCCGGAGTTGTGGCATTATTGTGGTGAGC 2282 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 601 GATGCTGACCGCAAGGT--AGGCCGGAGTTGTGGCATTATTGTGGTGAGC 648 gRNA GCTGACCGCAAGGTAAAGGC Wildtype 2283 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC 2332 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 649 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC 698 Wildtype 2333 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT 2382 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 699 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT 748 Wildtype 2383 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT 2432 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 749 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT 798 Wildtype 2433 CAG 2435 \|\|\| MutationRev 799 CAG 801	Morphology : Elongated internodes; rippled or wavy leaves

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0138

RG-PtoR

CTG0138-7

CGR0138-7

CRT0138-2 (T0 )

-1 bp (49.5%) / wt (45.1 %)

CRT0138

RG-PtoR

CTG0138-7

CGR0138-7

CRT0138-2-4 (T1 )

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

CTGACACTTTTATCACAAGTTCTTATACTTGCTCACTTACTCATCAGTGAGGAATTCAAGATTATACGGCTTATTCATGT
TTTTCATCTTTAGAACTTGTTTAGTTGTAGAGGCTTATTCCTTTTTATCTCAACAGGGAACGTGTATTCTTTGGCTCACC
ACAATAATGCCACAACTCCGGCCTACCTTGCGGTCAGCATCAACATGTTTGTGATGGAACAACAGCAGCCCAACTTGCTA
TACTTTTGTGTTCTTTGGGGCTTATGTCCATTGGTACTGGTTTTAACAGACCTTGTTCCATAGCATTTGGTGCTGACCAA
TTGGAGAACAAAGAAAATCCAGATAACGAGAGGCTTATAGATAGCTATTTCAACTGGTATTATGCCAGTGTAGGAGTTTC
ACTTGTTCTTGCAGTTACTGTAATTGTTTATATCCAAGACCATTTTGGTTGGCAAATTGGGTTTGGTGTCCCTGCTTTGC
TCATGCTTATATCTGTTTCCATTTTCTTGATCGGATCTCCTCTTTATATCAAAGCAAAAGCTAGAGGGAGTTTGTTCACT
GGATTGTTTCAAGTAGCAGTGGCAGCCTTCAGAAAAAGACATATCAATGTTGAGTCAAATTATATTGCTGACTGCTACTA
TAAAGAACCTGAATCGAACCTGCTCGAACCATCTGCCGACTTCAGGTATAGGTGCCTCCATCTTTTCTTCAATGGTATAG
ACATCTTCAACATCCATTCATGCACTTAGTATCTTGCAATACTAATTTGTTATACTGTATTACATGATGAACTCGTGTGA
T

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        1633 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA   1682
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 ATCACACGAGTTCATCATGTAATACAGTATAACAAATTAGTATTGCAAGA     50

Wildtype        1683 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG   1732
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 TACTAAGTGCATGAATGGATGTTGAAGATGTCTATACCATTGAAGAAAAG    100

Wildtype        1733 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT   1782
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 ATGGAGGCACCTATACCTGAAGTCGGCAGATGGTTCGAGCAGGTTCGATT    150

Wildtype        1783 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA   1832
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CAGGTTCTTTATAGTAGCAGTCAGCAATATAATTTGACTCAACATTGATA    200

Wildtype        1833 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA   1882
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      201 TGTCTTTTTCTGAAGGCTGCCACTGCTACTTGAAACAATCCAGTGAACAA    250

Wildtype        1883 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA   1932
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      251 ACTCCCTCTAGCTTTTGCTTTGATATAAAGAGGAGATCCGATCAAGAAAA    300

Wildtype        1933 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC   1982
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      301 TGGAAACAGATATAAGCATGAGCAAAGCAGGGACACCAAACCCAATTTGC    350

Wildtype        1983 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG   2032
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      351 CAACCAAAATGGTCTTGGATATAAACAATTACAGTAACTGCAAGAACAAG    400

Wildtype        2033 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC   2082
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      401 TGAAACTCCTACACTGGCATAATACCAGTTGAAATAGCTATCTATAAGCC    450

Wildtype        2083 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT   2132
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      451 TCTCGTTATCTGGATTTTCTTTGTTCTCCAATTGGTCAGCACCAAATGCT    500

Wildtype        2133 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA   2182
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      501 ATGGAACAAGGTCTGTTAAAACCAGTACCAATGGACATAAGCCCCAAAGA    550

Wildtype        2183 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT   2232
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      551 ACACAAAAGTATAGCAAGTTGGGCTGCTGTTGTTCCATCACAAACATGTT    600

Wildtype        2233 GATGCTGACCGCAAGGTAAAGGCCGGAGTTGTGGCATTATTGTGGTGAGC   2282
                     |||||||||||||||||  |||||||||||||||||||||||||||||||
MutationRev      601 GATGCTGACCGCAAGGT--AGGCCGGAGTTGTGGCATTATTGTGGTGAGC    648
gRNA                    GCTGACCGCAAGGTAAAGGC

Wildtype        2283 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC   2332
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      649 CAAAGAATACACGTTCCCTGTTGAGATAAAAAGGAATAAGCCTCTACAAC    698

Wildtype        2333 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT   2382
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      699 TAAACAAGTTCTAAAGATGAAAAACATGAATAAGCCGTATAATCTTGAAT    748

Wildtype        2383 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT   2432
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      749 TCCTCACTGATGAGTAAGTGAGCAAGTATAAGAACTTGTGATAAAAGTGT    798

Wildtype        2433 CAG   2435
                     |||
MutationRev      799 CAG    801

Morphology : Elongated internodes; rippled or wavy leaves

Feature Information

Sequence & Structure:

Name: Solyc04g005070 (Exon Intron) Length: 3404

The mutation and phenotype for selected feature: Solyc04g005070

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc04g005070 (Exon Intron)
Length: 3404