Name: Solyc04g009640
(Exon Intron)
Length: 3716
AGAGATGCCAAAAAAGAAAGACATTTTTTTAAAAAAACAGACAAATGAAAAGTATGACAAACAGATTGATTGAGTATCAACTTACAAACTTAAAAGAAAAAAACAACACAAAGAGATTCTAATTTACTTCTATGTTTCCAAATGTGTTCTTTATTTTGTTAAGCCTCTTGACTACATCTTTCATGGTTATCCTTGATTCT
GGTGTTGCCTTTGTGCAATCTAAAGCCAGTTCTATCATGGAGAGTATGCATATTTCACTTTCGGAAGTAATTTTTTCTCCATCATGAAACATATCCGCGT
CCACAACTTCCATCATAGTTCTTGGAAATGCTCGCGTTATCCACTCCCTCAAGCCAAGATTTTCGTTGAATATCTCTTCACCTGTTGGCCTTCTTTTTGC
CAAAACCTCCATCAGCATGATGCCATAACTGTAAACATCACCACGAGTGGACACTATTCCCTCCGAGCCATATTCTTAAAAGAACGAAAATGAAAAAAAG
TATTACTCAAATACTCATCTGATATAACAATAACGAAAGGTCTTCGATACATAAATGAAATGGAAAGAGACTAGTATAGTAGTATATATACCTGGTGCAA
TATATCCAAGAGTGCCTAATGTCTTTGTATGTGCCATGGATTTGCTTACAGCTAAAATCTTTGAGATGCCAAAATCACCAACACGCGCCACCATATCTTC
ATCCAAAAGAACGTTGGCTGGCTTGAGGTCACAATGAACTATTGGGGTGTCATTACCATGATGTAGATATTCAATTGCCATAGCTACATCAAGCATTATG
GTGACTCTTTGACGAAGTTTTAAGTGGCGATCTTCTTTGTACAACCAATTGTCAAGACTTCCGTTGGGCATATACTGCAGAACAAAGGCTCTTATGTGTT
CACTAGAACACGTAGTGATCACCGAAACAAGGTTTTTGTGTCTAACATTTCTCATCACTTCGCATTCAGTATCAAACCTTTTGCATACTTCCTCATTTTC
CAAATCCAGTACCTTAATTGCAACCACAATTCCACTAGCTAATGTGGCTTTGTACACAGAGCCAGAACCTCCCACGCCAATCAAATTGGATTCATCAAAA
TTGTTAGTTGCTCGTTGAATCTCATGATAAGAAATCAATTGATAAGTCCTCATCTCCGGAACCTTTTCAACATCTTTGGACTTCCCTTTCTTCTTTCGTT
TCATTATCCAAATCGAGACAACCAACAAGAATATCAGAAAGAATGAAATAACCATCGGAGTGACAATTTTTAGCACAAGCTTCTTCGATTTTGATTGTTG
TTGTTCAGGAGTAGTGATAGCACAAGCAGGAACCTCCAATATTTGCCTTCCACATAGACCTTTGTTCCCACTAAATGATTGCAGGGTGGAGTTTGCAAAC
ACACCACCACTAGGTATTTCACCTTCTAAAACATTAAACGAGACGTTGATGCTTGTAAGGTACAAAAGCTTTTCGAAAGACTTAGGAATAGTACCTGACA
AGTTATTCAAAGACAAATCCAAGAATTCCAAGCTTTTCAAGTTTGAAAAGGAAAATGGAATTGGACCTGAAAACGAATTGTTCGATAAGGAAAGTAACTT
CAAGGTTTGAAGTTCCCCCAAATTGCTTGGTATCGACCCCGAGAAATGGTTACCAGAGATATCGATTGCTACAATAGCTTTAAGTTCTCCGATATCTGAT
GGAACTTCTCCCTTTATAGAATTACGCGACATGTTTAAATATAGAAGACTACTCATCTTCCAAAAGCTTAAAGGTAGCTTTGATTCAAACTTGTTAGAAC
CTAAAAAAAGTTGTTGTAGCATGGTAAGATTTCCTAAACATTCCGGAATCACCCCCGAGAGCTCATTTTCAGACAGAGCTAATCGAACCAAATTCGATAA
ATCACATACTACCTCTGCTATGTCCCCCTGTAGTCCATTGTTAACTAGAAATAAACCTTGGAGTTGTTTAAGCTTACGGATCTCACGAGGAATGTTTCCT
GTCAAACTGTTATCTTGAAAAACTAGCGTTGTAAGACCGGTCATGTTGCCTGTACTAGTGGGAATGAATCCATTGATTTGTGCATCTCCAATATGAAAGT
ATTCAACAGTAGATGAAAGATTGCCAATACTACTTGGCAGAACATCATTCAACGGATTCGATCCCACTTGTACATATCTCAACATCCTACATTTCACCAA
AGAATCAAAGAATCCCAACTCAGTAAGTTGATTATGATGTAGGAAAAGATCTCGCAGCTCGCGAAGATTTCCTAAATCATTAGGAATAGTACCTGTGAGA
AGGTTATCTGATAGCTCTAACAGCTCAAGATTCGTAGCATTTGTGATGTACCGAGGAATTTCACCTTCGAGCTGATTGTTTGCTAAGAGAAGTTGTATAA
GATTTGGAAGGTGAAGAAGATTCGGAATTCTACCTGAAAGGTAGTTGAAAGTGAAAGCAATGAACTCCAAAGAAGATATGTTGAAAATAGTATTCGGAAT
TTCACCAATAAGATTATAATTTTCTTCGAAATTGATTTGTCTTAAGTTTGATAGCTTTCCTAATTCTGGAGGAAGTTCTCCCTCCATGTGATTGCTTCCG
CAATGAAGATTTTGAAGAGTTGAAATATTGCCTAATGAAGCAGGAATTGTTCCATTTATTGCATTATAACCGATATAAAACTCTTCGAGCTTGGCTAAAC
AACCAATATTTCTCGGTATTTCTCCAGTTATGTTGTTGTATGATATGGACAGAACTCTGAGAGCTGTGAATTGACAGATGTTGGAAGGAATGTGACCAGT
GATTTGATTGTATGATACACCTATATGCTCGAGGTTTGATGAAAGAACGTTACGTCGATCATCAGGAAACAGAGGACCTGAAAGGCTATTGTATGCCAGA
GACACGACGAGAAGGGAGGAGATATTAAACAATGTTGAAGGAATGGAACCTGTTAATTGATTACGAGACAACGACAACTCAACAAGTTGGCTTAGATTAC
CAATCTCCATTGGAATGTTGCCGTTGATTCTATTCCCATTCAAACTGAAGTTCATCAACTTTGTGGCATTTCCAATAGAAGGAGGGATCACACCTGTAAT
GGTATTGTTCCTGAGATTTAAGACTCTGAGTTCGGGTACATACCATGTACCGTTCCACATTTCACCACCGAGTTTATTGAAAGCCAATGAAATGATTTGA
ACACTCCCGTGTTGAAATAGACTTGTTGGAATACTTCCATGCAGCTGATTGTTCTGAATATCAATCACTCGTAGACGAGGCAAGCGGCCAATGCCATCAG
GGATTTCACTGTGTAAGTTGTTGTTTGCGAGATTTAGCTCTATGAGAAAAGATAGATTGGCCAAAGACGGCGAAATTGTGCCTTGAAGTTGCAAATTAGG
AAGAGTCAAGGCTACAACCCTTTGCCTTTTTGGACTACAAGTGACACCAAACCAAGAGCAAAATGAAGTGTTTTTTGTCCAATTATTGGCTAAAAAATGA
CTAGAATCACTCGTAACAAGGTTTCGAAAAGCTAGTAGAGCTTCTTGATCAGTCTCATTTGAGGAAATAGTAGCTGATATAGAAGAAACAAAGTAAAATT
GAACTAAGATAGCAAGTATCAATAAGAAAATGTGTTTCTCCATGATGTTACTAAGCATTTGAAAGTTGAAATATATGAAGAGAAATGGAAAAAGGATATA
TTGGAATGAATGACTC
The mutation and phenotype for selected feature: Solyc04g009640
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0009 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0009-13 (T0 ) | WT (47.8%); +1 bp (51.7%) | |
| CRT0009 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0009-6 (T0 ) | Large deletion; heterozygous | |
| CRT0009 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0009-9 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: TATGGTCATATAAATCAAAATAGAAACACAAAACGACAACTTTTCGTCTTGTGAAATAACTCAAGTGGAGTCATTCATTC CAATATATCCTTTTTCCATTTCTCTTCATATATTTCAACTTTCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTT ATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTTCTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAG AAGCTCTACTAGCTTTTCGAAACCTTGTACGAGTGATTCTAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCAT TTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGCAAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAA GGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTCATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAAT CCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGATTGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAA GTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGGCTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGG TATGTACCCGAACTCAGAGTCTTAAATCTCAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0026 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0026-4 (T0 ) | heterozygous | |
| CRT0026 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0026-4-3 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: TCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTTATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTT CTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAGAAGCTCTACTAGCTTTTCGAAACCTTGTCTACGAGTGATTC TAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCATTTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGC AAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAAGGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTC ATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAATCCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGAT TGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAAGTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGG CTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGGTATGTACCCGAACTCAGAGTCTTAAATCTCAGGAACAAT ACCATTACAGGTGTGATCCCTCCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0026 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0026-4-3-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTTATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTT CTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAGAAGCTCTACTAGCTTTTCGAAACCTTGTCTACGAGTGATTC TAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCATTTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGC AAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAAGGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTC ATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAATCCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGAT TGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAAGTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGG CTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGGTATGTACCCGAACTCAGAGTCTTAAATCTCAGGAACAAT ACCATTACAGGTGTGATCCCTCCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: "flgII-28 insensitive, segregating for flg22 sensitivity" |
| CRT0026 | RG-prf3 | CTG0009-1 | CGR0009-1 | CRT0026-4-3-1-1 (T3 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: TCAAATGCTTAGTAACATCATGGAGAAACACATTTTCTTATTGATACTTGCTATCTTAGTTCAATTTTACTTTGTTTCTT CTATATCAGCTACTATTTCCTCAAATGAGACTGATCAAGAAGCTCTACTAGCTTTTCGAAACCTTGTCTACGAGTGATTC TAGTCATTTTTTAGCCAATAATTGGACAAAAAACACTTCATTTTGCTCTTGGTTTGGTGTCACTTGTAGTCCAAAAAGGC AAAGGGTTGTAGCCTTGACTCTTCCTAATTTGCAACTTCAAGGCACAATTTCGCCGTCTTTGGCCAATCTATCTTTTCTC ATAGAGCTAAATCTCGCAAACAACAACTTACACAGTGAAATCCCTGATGGCATTGGCCGCTTGCCTCGTCTACGAGTGAT TGATATTCAGAACAATCAGCTGCATGGAAGTATTCCAACAAGTCTATTTCAACACGGGAGTGTTCAAATCATTTCATTGG CTTTCAATAAACTCGGTGGTGAAATGTGGAACGGTACATGGTATGTACCCGAACTCAGAGTCTTAAATCTCAGGAACAAT ACCATTACAGGTGTGATCCCTCCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: flgII-28 and flg22 insensitive |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
