Name: Solyc04g014890
(Exon Intron)
Length: 3302
TCATCTGTTGGCCTTCGTCAAAATCTCCATCAACATGATGCTGTAGCTATAAACGTCACCGCTAGCAGACACTATTCCCTCTGAGCCATATTCTTAGAAAAGGATTGAACATAAGTTACTCAATTGAAACACAACAATTACTCTGCATAAATGAAGACTTGCTTTTTTTACTTCTAGAATGCTAAACTATGATAAGAGAA
AATCAAAGAGAGAATTTTCTTTATACCTGGTGCAATGTATCCAAGAGTGCCCAATGTTTCTGTATAAGCCATTGACTTGCTTATGGCTAAAATTCTAGAG
ATGCCAAAATCACCAACATGAGCCACCATATCTTCATCCAAAAGAATGTTGGCTGGCTTTAAGTCGCAATGAACTATTGGAGTTACATGACCATGATGTA
GATATTCAACTGCCATAGCTGCATCAAGCATTACAGCAACTCTTTGATGAAGGTTCAAGTGCCGATTCTCTTTGTACAACCAATTCTCAAGACTTCCATT
GGGCATAAAATGCAGAACAAAGGCTCTTATATAGTCACTAGAACACGTAGTAACCACAGAAACAAGATTTCTGTGTCTAACATATCTCATCACTTCACAT
TCGGTATCAAACCTCTTGCATACTTGCTCATTTTCCAAATCCAGAACCTTAATGGCCACTGCAGTTCCACCAAACAATGCACCTTTGTACACAGAGCCAG
AGCTTCCCTCACCAATTAAATTGGATTCATCAAAATTATTTGTTGCTCGTTGAATCTCGCTGTAGGGGCAGCGATTTAAAAAAAAATTAAAAAATATTAA
ATCGTTGTTGGGCAGCGAATTAAAAGGGGCAACGATTTCTTTATTTTTTTGAATTTTCTTTTTGAAGAAATCGCTACTCCCCAGCAGCGATTTTACTTTT
TATTTTAAAAAAATTAGATTTTGCAAAATCGCTGCAGAGGCAACGATTTTGCTTTTTTCGGTGGGGCAAGTCGCTGTTGGTGCAACGATTTTACCGTTTT
GATTAATACAAAATTTTATATACCGTTTTAGAATTTTTGTTAATATTTTGTACCTTTAGGCTCCGGACTCCACATATTATAGGATCGTAGAGGCGTGGAC
TTTTAGAAAGTCATCGTAGTAATGTCACTTTGTGGTAACAATTAAAATAATAAGGTTTAATCTCACTTTTATGGTAACAATTAAATTGAAAAAAATGAAA
AGTCATGCAAATATCTAATACAAGTGACCAATGAATAACTATATGATTCCCATATCTATATTTGATAATAGGGTAAAATTACTTGGTGTTTCAATTGATT
TACAAATTTTGGAGTACACATATCACCCTTCATTGTTTTAAGTTTACGAGTATATTTCTTTTTTTAATAAATCCTACTTTATGTGTCATTTATATACTTT
ATACATGTCTCATTTTTAAAAACTCAATATTAATTGATCAGGTACACATGCGACACATGTGAGCAACGACTGGTATTATTAAATAACATGATTTGAGTTC
TTTTTTGGTTAAAATGTGTCCAATTGAGATTTCTTACTTTGTCTCATTATTAATTGTAATACTCCAAATTCCTTACCTCAAAGTGAATGCATCGAGATAA
TGATATGAGAAGTTAATAATAAGTCATAAATGAGATTATAAAACAAAAGGGAAAAGATAACAGCCAGTCAAATTAAATTTCTTGATAGTGTTTAAAGTTC
CTATGCAAATCGTGGTGCAATATTAATGTTTCATCAATTAAAAGTTTGATAGGAGATTATTTACTTTCTGTGTAATTATAATGGATGTGTATAAAATATT
GTATTAAGATTTTATCCACAAACACTTTAAACTAATTATCTAATCACAACTCTAGGGAAAAGTAGAAGAGTAAGACTAAAAAAATTATAGTTGAAAACTG
AAAGGAAATTTCTTTATTTATCGTCAATAATAGATAGTATGAATAAATATTTTTGTGTCTTATTTGAAAAGTCATAACCTTTTCGAGAAGTCACAATTCT
TCTGAAAAGTTAAAACTTATTGAAAATTAAAAGTCACAACTTATTGAAAAAGACACAACCCTATGGAAAAGTTTCAACTCTTTGGAAAAGTCGCAACTCA
TCGAAAAATCATAATCCTTTCAAAAAAGTTACAACTTATTGAAAAAGTCACAACTCATCAAAAAAATCACAACCTAAATTAGAAATATCGTAATAATTTA
ATATTTAAATTAGGAGTTCATACTTTTAAAGTAAATAGATTGTGATTTCCTCAAACTTTTATGTGATGTTATTGATATGTCATCTCATTGAGGAATATTA
CAATGGCCAACTTTATAAAGACAAAACCACAAGAAAGACAGGTCAATTATGCGTTAACTGAGGGGTAAGGGAATTGATTGAGAAAAGTCAGTCAAACTTG
TATCAGTGAAATCACTTTTTTTAATTAAAAAAAGTTGCATATATGGTCCAAAACCAAATGAAATATCTTCACCTAATATTTGAAGGGTATTTCTCTAGTT
ATGTTGTTGAAAGAAATGAATGAAATTAGATAGTAAGATATATAGTTTTTGGCTAAAATCATTTATCAACTATGATTCAAAATTAATGTATATTTTTATA
TTATCTAAGCGAATAAAAAACATCCTTAAACGATCCAAACAATAATATGAACCATCCTTCAGTATATTTTAGTTAAAAAAACTGATAGAACCATTAATAA
TATATTTAATTTGATCATGTCTTGACCATGAGAATCATCTCATTTTTGGCGAACTTTTTAAAGGCAAAAACACATGGATATATATTGTATGGTCAATGGA
AGTCTCCACAGTCTAAGATAAGTTTAGGAAAAAAACATGTCGTTTGACCATCAGTTATTGGGACAATACAAAATTTATTTGTGAAATTTTACTAATGGTA
ATAAATAGTGTATATGAGCCTGTAACTTTAAAATATATAATGTATTTAATTTTTATACAGGTAAATATTGAATCTGTCTTTTGATCACGTATCAATAATT
GAATAATCTATAAAATGCTGTTGCAACAAACACATTTTGTAGAAATAAATTATAAATTATTTAAGTGTAGTTTTATTTTCGGATAAAAACAAATTGGTTT
TGAACAGATAAATAAAGAGCTAACAAAGAAGATATCAACGGTAAATATTGTTAGTATCAAGACCAAAAGTTCAGGCATGATGGTACACACCCCAGAATTT
CAAAAAAAATGTGTGTAAGCCGAAAGAATACATAAGAAACTACCGAAGGGAAGTCAGACCACAACACCTGATAGCATAAGTATAAAGAGCATCTGATACC
AT
The mutation and phenotype for selected feature: Solyc04g014890
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0137 | M82 sp+ | CTG0137-21 | CGR0137-21 | CRT0137-11 (T0 ) | +1 bp/+1 bp | |
| CRT0137 | M82 sp+ | CTG0137-21 | CGR0137-21 | CRT0137-11-3 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT TGAGAATTGGTTGTACAAAGAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : "Stunted, not bleached" |
| CRT0137 | M82 sp+ | CTG0137-21 | CGR0137-21 | CRT0137-11-3-1 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT TGAGAATTGGTTGTACAAAGAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0137 | M82 sp+ | CTG0137-21 | CGR0137-21 | CRT0137-11-3-2 (T2 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT TGAGAATTGGTTGTACAAAGAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0137 | M82 sp+ | CTG0137-21 | CGR0137-21 | CRT0137-11-8 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: ACAACGATTTAATATTTTTTAATTTTTTTTTAAATCGCTGCCCCTACAGCGAGATTCAACGAGCAACAAATAATTTTGAT GAATCCAATTTAATTGGTGAGGGAAGCTCTGGCTCTGTGTACAAAGGTGCATTTGTTTGGTGGAACTGCAGTGGCCATTA AGGTTCTGGATTTGGAAAATGAGCAAGTATGCAAGAGGTTTGATACCGAATGTGAAGTGATGAGATATGTTAGACACAGA AATCTTGTTTCTGTGGTTACTACGTGTTCTAGTGACTATATAAGAGCCTTTGTTCTGCATTTTATGCCCAATGGAAGTCT TGAGAATTGGTTGTACAAAGAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : "Stunted, bleached plants" |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
