Plant Crispr Database

Feature Information

Feature ID: Solyc04g049090
Feature Type: gene
Name: SlMlo1
Description: Mlo-like protein 1
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc04g049090

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation
CRT0023	RG-PtoR	CTG0023-2	CGR0023-2	CRT0023-1 (T0 )	-2 bp (48.2%); -7 bp (43.4%)
CRT0023	RG-PtoR	CTG0023-2	CGR0023-2	CRT0023-1-5 (T1 )	-7 bp/-7 bp Is tDNA present: No Mutation sequence: CTCGCTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACT AGATCGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAA AGTCTCTATATGAAGCACTTGAAAAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATGG ATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAGT GTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGATG CCAAGTCTGAGTAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4844 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC 4893 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 3 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC 52 Wildtype 4894 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA 4943 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 53 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA 102 Wildtype 4944 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC 4993 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 103 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC 152 Wildtype 4994 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA 5043 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 153 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA 202 Wildtype 5044 TACAAGACTGTATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCA 5093 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 203 TACAAGACTGTATTTTGAATTTACCAG-------CTTTTCAAGTGCTTCA 245 gRNA GCTTTGATCTTTTCAAGTGC Wildtype 5094 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT 5143 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 246 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT 295 Wildtype 5144 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT 5193 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 296 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT 345 Wildtype 5194 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA 5243 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 346 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA 395 Wildtype 5244 ATAAAAATAGAAATAGCGAG 5263 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 396 ATAAAAATAGAAATAGCGAG 415
CRT0023	RG-PtoR	CTG0023-2	CGR0023-2	CRT0023-1-9 (T1 )	-2 bp/-2 bp Is tDNA present: No Mutation sequence: CTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACTAGAT CGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAAAGTC TCTATATGAAGCACTTGAAAAGATCAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATG GATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAG TGTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGAT GCC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4855 GGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACCAACACTCTTGG 4904 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 GGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACCAACACTCTTGG 50 Wildtype 4905 GGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCAACAACAGTGAT 4954 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 GGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCAACAACAGTGAT 100 Wildtype 4955 AAGAATCCCAACAGCATAAGTTCTGTCCGACACACACACACCAAAAATGT 5004 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AAGAATCCCAACAGCATAAGTTCTGTCCGACACACACACACCAAAAATGT 150 Wildtype 5005 CATGAGTATGATCCATCTTTAAACTATATTATTTTATAATACAAGACTGT 5054 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CATGAGTATGATCCATCTTTAAACTATATTATTTTATAATACAAGACTGT 200 Wildtype 5055 ATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCATATAGAGACTT 5104 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 ATTTTGAATTTACCAGC--TGATCTTTTCAAGTGCTTCATATAGAGACTT 248 gRNA GCTTTGATCTTTTCAAGTGC Wildtype 5105 TTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTTTTCCCAACAAT 5154 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 249 TTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTTTTCCCAACAAT 298 Wildtype 5155 TAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGATTTTGAAATTAT 5204 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 299 TAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGATTTTGAAATTAT 348 Wildtype 5205 ATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCAATAAAAATAGA 5254 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 349 ATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCAATAAAAATAGA 398 Wildtype 5255 AATAG 5259 \|\|\|\|\| MutationRev 399 AATAG 403

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0023

RG-PtoR

CTG0023-2

CGR0023-2

CRT0023-1 (T0 )

-2 bp (48.2%); -7 bp (43.4%)

CRT0023

RG-PtoR

CTG0023-2

CGR0023-2

CRT0023-1-5 (T1 )

-7 bp/-7 bp

Is tDNA present: No

Mutation sequence:

CTCGCTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACT
AGATCGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAA
AGTCTCTATATGAAGCACTTGAAAAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATGG
ATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAGT
GTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGATG
CCAAGTCTGAGTAAT

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4844 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC   4893
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        3 TACTCAGACTTGGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACC     52

Wildtype        4894 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA   4943
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       53 AACACTCTTGGGGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCA    102

Wildtype        4944 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC   4993
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      103 ACAACAGTGATAAGAATCCCAACAGCATAAGTTCTGTCCGACACACACAC    152

Wildtype        4994 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA   5043
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      153 ACCAAAAATGTCATGAGTATGATCCATCTTTAAACTATATTATTTTATAA    202

Wildtype        5044 TACAAGACTGTATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCA   5093
                     |||||||||||||||||||||||||||       ||||||||||||||||
MutationRev      203 TACAAGACTGTATTTTGAATTTACCAG-------CTTTTCAAGTGCTTCA    245
gRNA                                           GCTTTGATCTTTTCAAGTGC

Wildtype        5094 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT   5143
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      246 TATAGAGACTTTTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTT    295

Wildtype        5144 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT   5193
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      296 TTCCCAACAATTAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGAT    345

Wildtype        5194 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA   5243
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      346 TTTGAAATTATATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCA    395

Wildtype        5244 ATAAAAATAGAAATAGCGAG   5263
                     ||||||||||||||||||||
MutationRev      396 ATAAAAATAGAAATAGCGAG    415

CRT0023

RG-PtoR

CTG0023-2

CGR0023-2

CRT0023-1-9 (T1 )

-2 bp/-2 bp

Is tDNA present: No

Mutation sequence:

CTATTTCTATTTTTATTGAACAAATTATTCATCACATTGGAGAGGTACCTACTATATAATTTCAAAATCTTTTACTAGAT
CGATATGAACAAAATATGCTTATTAATTGTTGGGAAAACCATTTTCTTTTTTAGTGGTTACTGGAAAAGCGGAAAAAGTC
TCTATATGAAGCACTTGAAAAGATCAGCTGGTAAATTCAAAATACAGTCTTGTATTATAAAATAATATAGTTTAAAGATG
GATCATACTCATGACATTTTTGGTGTGTGTGTGTCGGACAGAACTTATGCTGTTGGGATTCTTATCACTGTTGTTGACAG
TGTTGCAAGATCCAGTTTCTAACTTATGTGTCCCCAAGAGTGTTGGTTATTCATGGCATCCTTGTATGGCAAAGGAAGAT
GCC

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4855 GGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACCAACACTCTTGG   4904
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev        1 GGCATCTTCCTTTGCCATACAAGGATGCCATGAATAACCAACACTCTTGG     50

Wildtype        4905 GGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCAACAACAGTGAT   4954
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev       51 GGACACATAAGTTAGAAACTGGATCTTGCAACACTGTCAACAACAGTGAT    100

Wildtype        4955 AAGAATCCCAACAGCATAAGTTCTGTCCGACACACACACACCAAAAATGT   5004
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      101 AAGAATCCCAACAGCATAAGTTCTGTCCGACACACACACACCAAAAATGT    150

Wildtype        5005 CATGAGTATGATCCATCTTTAAACTATATTATTTTATAATACAAGACTGT   5054
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      151 CATGAGTATGATCCATCTTTAAACTATATTATTTTATAATACAAGACTGT    200

Wildtype        5055 ATTTTGAATTTACCAGCTTTGATCTTTTCAAGTGCTTCATATAGAGACTT   5104
                     |||||||||||||||||  |||||||||||||||||||||||||||||||
MutationRev      201 ATTTTGAATTTACCAGC--TGATCTTTTCAAGTGCTTCATATAGAGACTT    248
gRNA                                GCTTTGATCTTTTCAAGTGC

Wildtype        5105 TTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTTTTCCCAACAAT   5154
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      249 TTTCCGCTTTTCCAGTAACCACTAAAAAAGAAAATGGTTTTCCCAACAAT    298

Wildtype        5155 TAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGATTTTGAAATTAT   5204
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      299 TAATAAGCATATTTTGTTCATATCGATCTAGTAAAAGATTTTGAAATTAT    348

Wildtype        5205 ATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCAATAAAAATAGA   5254
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev      349 ATAGTAGGTACCTCTCCAATGTGATGAATAATTTGTTCAATAAAAATAGA    398

Wildtype        5255 AATAG   5259
                     |||||
MutationRev      399 AATAG    403

Feature Information

Sequence & Structure:

Name: Solyc04g049090 (Exon Intron) Length: 5507

The mutation and phenotype for selected feature: Solyc04g049090

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc04g049090 (Exon Intron)
Length: 5507