Name: Solyc04g082260
(Exon Intron)
Length: 5842
TCACCAATGTATCTTCACCACTCTATTGTATTAACAAATATACATACAAATGTACACATTGAGAAATTTATTTTTCATATTTTGACAAATCAGAGTGCAAAACTGACATACGCATATACCAATAGAGCAAATGATTCTCTTCCTTTGTTATACATAGTTCTAAGACAATCACACTCAAAACAAATGATACAGAAATTTGG
GCTAATTTTGTGATGACAAGAATAACTGATAGGGCCAAAAGAATGATACAAGAGAGAGTATTATCAAGATGCACGCCCTCCGCGTCTTTTCTCTTCTAGA
ATTGCAGCCTCATTCAACATGTCAGCTGCATCTTTGTGCATGTCTAGCTTCGACAGGGCAACTGCCTGCATGTAAAATGCAGTTGACCAGTCTGGGTGAA
CACATTGTGCTTGCATTGCATCTCTAAGGGCAGCATCTGGTTGATCACACATGAGATAACAAAGGCTTCTTCGTGCATAAACAGTTGGAGACACCATCGT
TCCCACATCTACAAACTGCAAAGAGACACAGAAATAAGCAGATTGCCTAAAGCAAAGTTGTATTTAGCTCATCCATCTTACGCGCACAAGTAAGCTAATG
AAAGAAATCTGTTAACCCAAATGCAAGCTAGATCATCATGCTACCTAATAACCGTGTATTAAGGGAATGCAGGAAATCTATACGACTACTCCAGCAGCTA
GAAAAGGTAATGCAGTAAAGCATCCAATGAAGCAGCAAGTTTCTGGCCACATCTTCGCTATCTCATTCTCAGAAAAGGTCATGCTACAATATTGGCATTG
GATGCAGGGATAAAGCTAGAAATATGGTTTGTAATCAAATCCTGGGTCCACCTCTGATTGGATGGAGTCTATAGATTGGTTGTTCTATTTAACATGGCAG
ATTTACCAGGCACGCAAATAATTGTGTTACATCCCCGCGAACAAGATCAGCTTACAGTATGGAATTAGATTCCAAGAGTGCATTGACTTTATTCCTTGGA
TTTTTGGCCAAGGAAGATTCAGCGGTGACACAAATAACCAAAAATGTCAACTAGATCACATACAGTATAGAATTAGATGTGGAAAAGACACCATAAACAC
AGGCCACATTCAGATTTTGGTCTTTCTATTAAGTGGTCGAGCATAGATGGCTCCAAGGAAACTGATTTTTTTTTCTGGAAAAGCCGCTAATTTTAAAATT
CTAAATTGGATACATGATAGAGACTTGGGAATGTCAATCGACCCACACAAGAAGAAGCAGAAGATCTGCCATTCCTATCACCAATGAAGATGCATGTCTC
AATTAATCAAGGAAGTAACTATTTATGGCATCCAACTACATAAATGGTATTCAGGAGTTAAGTATATTACGGTCTACCTCATGTCTTGTCTGGGTCAGTC
TTTTATTGCCAAATGCCAATACTGACCTTACTGGGTTAAGGAAGTTGCAAGTTGTAACCAGTAATACAGATTGACACATTCTCATTAGATGAAGGCAAAT
ACCTGAGAATAGCAATCTATGGCAGTTTTAAAGTCCTTGTCCCGAAATGCCAAGTCACCACGCTTTCTTGCCTCTAACATATCCCTCATTTGTTGAGTCC
ACTCTTGGAAAGACAACTGCATCATTGGGCATCCAACAACAACATAAAGATGTGAAAGAAACAGATGCTTCTGCAGTGGCAGTTTAGGTCAAAATAATTA
CCTCATTTGTCAGTTCATCGTCTTTATAATGTGTCATTACCAAAATCTGATGAATAGCTGTGAGATCCATTCTCGAACAAGCATCACCCATTGCAGAAAG
AGGGTGCTGTGGAGTTGGTGGAGCTTCCTCACTCTTGGGAATACCCAACATTACATGAGATGCAACCTGCAATCAAACTATCAGAAACTCCTTCTAACCT
CTAATTGGATAAAACAAATCAATCAATTAGATAAAAATAGACTCAAACTTCCCAGCAGAAGCAGTTGCTCGGGAACAAACAAGACATGGACACAGTATAC
AACGAGAATTGAAGATAAGGGAGCATCTTAAGATGAAGGAGAAATGAGGAGGCAATATTTGGTAGTTAAGCTTACCAAACAAATTTTTTACAATATGAGG
TTTTTATTTTCCCATATTCTTTGGAAGGGGAAAGGATAGAAAACAAGACATCAAGAAAAATAGAGCAGATTATGGTGGTAGGACTAACATCAGGTTTGCT
TTGCAATGGACCAAGTGTTGAAACGAGGTCTTTGGTATTTGGTCGCTCCCTGGGTTCATACTGTAAGCATCGTGAAGCCAGATCAAATACAACAGTTGCC
TCTTCTGTAGAGAAATTTCCCTCCAAATGTGAATCCATTAATAGAAGAATGTTTTTTCCCCGTATCATATCGAGTGCCTGAAACGTAAAGGAATGAACTA
AGCACGTAAGATTTAAATTTAAATTTTAAAACAGTTCACCATAGGAAAGGGGAGGAATTGCAAAGGAGAAATGAGGTAAACACAATGACACCAATATGAA
ATGTTCTACAAGACAAAGTGAAGAAATGTACTCTACACTAGTATTAACATCAAATGAATTTCTCCTCTTCTTTTAGAAAGATGATCAAAGACAGAAAATG
TATGCGGATGTATAATCAAAACTGTCTCAAACTGCTAATGTTCCAAGCCTAATGTCCAACAACCCTCCTATTGCTGGTCTGGCAACAAATTTTGTACATA
GTCTCAGACATTACAATTAGTGGTTCAATCAATAATTTCCAGAGTACCAGTACATAAATACAAGTTTACAACACAAGAGAGCCTAAAATAGTGTTCCCAT
CATTGACAAGGTGACAATCCCCAAGACTTGGTGCAAAAAGAGATGAATTTACTGCTAACAAACAGACTCTAAGAAACTTACATGACCAGGAGGAATATGT
TTTCCACTTAGCAAGTCCAAAAGGACAGTTCCATAGCTGAAAACAACACTTTCTTGAGTGACTCTTCCTGCAAATAGACAATAAATTGTTCATCCCTTAA
TATCAATTGACAATGAGAATGATAATAAACAGAAAATGATAGATATCAATAGAATCACTTAGGCAGATCATAATGGAATCTTCCTTTTTTCAGTTGTCAT
ATTTAAGTTCCATAACCCTTTCTCGGAAAAAGAAAAGAACCAAAAGCAGGAACATAACTTACTACCAAAACACAAATGTATAACACCACCAGCATCAGGA
GATGTAGGATTGGAATTCCAAAATCGCTCTGAATTGTGAACTTTCCTAGAGAGATATAGTCTCACCCCTACCCCCCACCAAGCTATCAGTTTACAGAAGC
CGATTACTATCAAGCAAAATTCTGAATATCTTGGTCAGAAATATAATTCAAACGAGAAAATGATGACATAATGTACATCAGGTTATTGGAGATGCTGAAG
GCACCAAATTCAGTTACTAACGTATTCACAACTTCTGTAGGAATTTCCTTCATTACCTCACATCACAGAACTTTGTATAACAGCATTCCGGTCTACTATT
TATTCGTGCATCAAGAGGTCATGTAACACTGTTGCATTTAAATATCAGGATGTGAATACTAATTTTGCTCCCTGGAAAAAGTTCACTGTGAACACCTAAT
GATAGATAACTCAAAAGGCAGGCACTAATTAAGAAGTATTGGACTCATATATATATTTCGAACAATCAACAAATTTAAGATTTTTCTACATATATATTTG
AGATGAGATATTACTTTATACACCATTGAAGTAGACATTTCATTCTGTTCATCCAGGGAGCTCAAGACGTTTTCTTGATTTACACATTTTATAAGTAAAA
AGCAATTAGCAAAGTTACTCCATACATCCTCTTACTTTAACACACATGGAATAGTGTCTCTTTTTAAATCAGCACCAGACAGCAGTCACAACCTCACATC
ATAAAATGGCTGTCTCGTGCAAGGAGACATACCCATCCAAAATGTTCCAGTCCCAGATAGCATCACTATCCATGGTAATAGTGCTAAGAGGAAAACTTTA
GAACTCAACACAGACAAAAACTTCAAAACTTTACCATTTCTTAAATACTCAGGAGGTGTATACGCAAGGTTCGTGCTATAACTTTTACCATCCCTGCTAT
TTTTCATCAGCCCAAAACAAGAAAGACGGGGATCGCCACTCTGCCAAAAATCATTTACAGAATTAATACAGTGATAAAAGTAAATTCAGGAGTCTACATT
AGACATTACTCTAAAATGTATGAAGACATTCCAAGCTCTAAAAAAGATGTCCATCAAAGTACCTCATCAAAGAGAACTCTATATGCATTCAAGTCATGGT
ATAGTGGTCGACCTTCACTGCTACAGTAGTCCAATGCTTCAGCAATATATAGAGCCACTCTTAAACGCATAGCCCACTCAAGGGTTTGATTCTCCCCTGG
AGAGAAAGAACAAAGAAAATGCATTTCCGCAGATCCCATGAGATGAAAGCAATATACAATATAAAGGTCAATATATTATATCAGATTAACTAGGAAAGCA
AAATTCATAAAGCGACAAAGCAAGAAAAAAAAATATGTTAACAACAGGTGTATCCCTAAATTTATGTATGAAGAAGATAACGACAAACACACATTGTCTT
TTATAACAAAGTTAAAGTTTTTAATGCCCCAACCATCACTCTGCCCGCTAATATGACAAAGAAGATGATAAACACTAGCTACGATACAAATTACCGACAA
AACCAAAAAACAACGTTTACATCTCCCCAACACCTATTGGAGCAAAGCAGTTAATGCTATATTACGCCTATCTCTAACTATCAACCATAAAATTATCTCG
ATTCTTCTCTTCATTTCCATAGATAAGCTGCATTGAAGCTAAAAACAAAAAAACAAAAGCAGTAAATGATAAACATACAGTGAAATAGATGCTTTGCAAG
TGTATCATTTGGCATGTACTCAGCTACAAGCAACCTCTCATCCCCATCAGAGCAGTACCCAATTAAATTAGCCAGCCTTTTATGCCTCAAATTTCCAACA
CCTGATGCTTCATCCTAATTCCAACAACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACAT
GGCTATATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAATAAAACAAGGGCAATACAGTAAAT
AAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTCTTAACAGCGATCCACCGCCGATTCTGCAACCTCC
CCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAATAAATTCTGAACTGAAGTTATTCGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTC
AGAAAACACCGGAACCCCACCATCTCCGACAGCAGCTCCGGCGCCGGTGCCGTTAGAAGCTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAG
CTCAACTCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTTAAACCTCACCCAAC
CAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTTTTTTAATCTTTTTTTTTTATTTTTTTTTTGGGAT
TATTATACAGCTAGAGAGATGAAATGGTGAGAATTGCAAAGTGAAAGTGAAAACTCAAAAAACTGTGCTTTTTCATTGACTCATTTGTCTCTCTCTTACA
CCTTTTTTCTGCTCTGACACAGCTTTCTCTGTTTTTTCTTTG
The mutation and phenotype for selected feature: Solyc04g082260
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10 (T0 ) | -4 bp/WT | |
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-20 (T1 ) | +1 bp/WT | |
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-43 (T1 ) | -4 bp/WT | |
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-43-8 (T2 ) | -4 bp/WT | |
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-46 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAATAAAACAAGG GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT AAATTCTGAACTGAAGTTATTCGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC CGACAGCAGCTCCGGCGCCGGTGCCGTTAGAAGCTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT TTTTAATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-5 (T1 ) |
-4 bp/-4 bp
Is tDNA present: Yes
Mutation sequence: ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAAAAACACAAGG GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT AAATTCTGAACTGAAGTTATTAGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT TTTTAATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : "Slow growth, sparse, brittle leaves" Immunity response: Inconclusive disease assays with Pst DC3000 due to leaf phenotype. |
| CRT0011 | RG-PtoR | CTG0011-1 | CGR0011-1 | CRT0011-10-5-1 (T2 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: ACATCAACAAAAATCAGAACAAATAGCAACAACACCTCAATCCTAAACGTGGAACCTTAGTAGCTCAGTTACATGGCTAT ATCCACTATATCCATTCAGGTCAATTTAGGTCAATCTCAAATCCACATTAAACCCTAGCTTCACAAAAAAAAACACAAGG GCAATACAGTAAATAAATCAAAGTAAGAGGGCAATACCGCAAACTGTTTAGGATCAGGCCATGCCGATTTAGTAAACTTC TTAACAGCGATCCACCGCCGATTCTGCAACCTCCCCTTGTAAACCATATTCGGAGCCTTTTCTCCACTTTCAGAAACAAT AAATTCTGAACTGAAGTTATTAGTAGCTGCTTTGAGCTCAGAAAGCGAAAACTCAGAAAACACCGGAACCCCACCATCTC CGACAGCAGCTCCGGCGCCGGTGCCGTTA----CTCGTGCGTTTACCACCCCATGACGCTGATCTTTCTCTGAGCTCAAC TCCTTCAAGATTGAAGATTGACAACAACCCATGATGACCCAAATCAAGAATCCCTTCAATTAAACATCATAACCAATCTT AAACCTCACCCAACCAAAAAAAACAACAAAAAAAGGAATACAACTTTATTATATGATTAAATTAATCATAAAAATAGCTT TTTTAATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
