Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
---|---|---|---|---|---|---|
CRT0138 | RG-PtoR | CTG0138-11 | CGR0138-11 | CRT0138-30 (T0 ) | wt (42.2%) / +1 bp (38.8%) / -9 bp (3.8%) | |
CRT0138 | RG-PtoR | CTG0138-11 | CGR0138-11 | CRT0138-4 (T0 ) | +1 bp/+1 bp | |
CRT0138 | RG-PtoR | CTG0138-12 | CGR0138-12 | CRT0138-19 (T0 ) | -1 bp (47.9%) / +1 bp (42%) | |
CRT0138 | RG-PtoR | CTG0138-12 | CGR0138-12 | CRT0138-19-2 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: GTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGTCCCTTTGCTATCAAAATATACT GGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCTGTATGTCTATGGCGTTGTCAGC AATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCAAGTGCGTTAGTGAACATGTCTG CTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGTGAGGGTTCCCATTCTGTTGGACAGATTGAGTTCTTCTACT CTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTGCTGTGTCGAGTTTGGTTGTAAGT ATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTTTCGAGCAACATTAATAGGGGTCA CCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTTTCTGCTCATCTGCCGGTTATATG AACCTG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Stunted growth; very branchy; smaller; Germination ~60% |
CRT0138 | RG-PtoR | CTG0138-12 | CGR0138-12 | CRT0138-7 (T0 ) | -105 bp/WT | |
CRT0138 | RG-PtoR | CTG0138-12 | CGR0138-12 | CRT0138-7-11 (T1 ) |
-105 bp/-105 bp
Is tDNA present: No
Mutation sequence: GATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGTCCCTTTGCTATCAAAATATACTGGACAGCCAA GAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCTGTATGTCTATGGCGTTGTCAGCAATAACAGAA AGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCAAGTGCGTTAGTGAACATGTCCAGCATGGCATC AGCTATGTACACTGTTGGGACTGCTGTGTCGAGTTTGGTTGTAAGTATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAA CTGGAGGCAAAACTAGCTGGCTTTCGAGCAACATTAATAGGGGTCACCTAGATTACTATTTCTGGCTACTTACTTTTTTG AGTTTGCTCAACTTCTTCTATTTTCTGCTCATCTGCCGGTTATATGAACCTGACAATGATGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Retarded growth; Germination: 75% |
CRT0138 | RG-PtoR | CTG0138-12 | CGR0138-12 | CRT0138-7-7 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: GAAATACCAGCAGCATCATTCAGTGTATTCATGATTATTGCTTTAACAATCTGGATTACCTTCTATGATCGTGTTTTGGT CCCTTTGCTATCAAAATATACTGGACAGCCAAGAGGGCTAAGTCCTGTCATCCGAATGGGTATTGGCTTAATAGTCACCT GTATGTCTATGGCGTTGTCAGCAATAACAGAAAGCATAAGACGACAATTGGCAATAGAGGAAGGACATGAGGACGACCCA AGTGCGTTAGTGAACATGTCTGCTATGTGGCTCGTACCACAGTATGCACTACTCGGAGTGGCTGAGGGTTCCCATTCTGT TGGACAGATTGAGTTCTTCTACTCTCTGTTACCCAAAAGCATGTCCAGCATGGCATCAGCTATGTACACTGTTGGGACTG CTGTGTCGAGTTTGGTTGTAAGTATTCTGGTGAGTGGTGTGGATTGGCTTTCATCAACTGGAGGCAAAACTAGCTGGCTT TCGAGCAACATTAATAGGGGTCACCTAGATTACTATTTCTGGCTACTTACTTTTTTGAGTTTGCTCAACTTCTTCTATTT TC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Retarded growth; Germination: 90% |
This database is supported by NSF (IOS-1546625) and hosted by BTI.