Name: Solyc05g005940
(Exon Intron)
Length: 3377
TACATAAAAAAATATATGTATAGATGAAGATTGTAGAGGTCTTAACATATGAAAAACTTTATTGTCCAACTCAATTAGTAGTTGTTGGATCATTCAAGGATGCAATACAATATATCATCAAGATTCATGTAAGAGTCTATAATCGCATTCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTAT
CATAAAACCGGCAGACAACTAGAAAATATAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCT
CGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTCCCAACGGTGTAC
ATAGCTGACGCGATGCTTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCCTCAGCTATTCCGAGTAGTGCAT
ACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCATGCCCTTCCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTTAT
TGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAA
GGGACCAAAACACGATCGTAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGATAT
GTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGACCACATAGGAAGTATTCTAATAAG
AGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGATCCATCAGGATTCAAATCTCTTTCAGGATCTTCAATCATACAA
GCTCTATTTAAACACCTACATATATTCATCATACAATAAACTCTTATTCTAATGAAGTGCAATATAATAAAAATTAGACATAAGACGGAGACACTGACCT
GAAGTCTTTTGATGGCGTTAAGACCTCTGTTTCATATGGCGAATGGTAGTAGTCATCACAATCAGTCAATGAAAGAGGGGTTTTTCTTTTTCTAAATGCT
GCTACACATGCTTGGGACAATCCTACTAGCAAGTTGTTTGTATCAGTTTTCACTTTGATATAAAGAGGAGAACCGATTAGGAACGTTGAGATAGACAAAA
CCATGAGTATAACAGGGACACCAAAGCCAACTTGCCAACCGTAACGATCTTGAATATAAATAATTACAGTAACTGCAATCATAGTTGAAACACCTGTGCT
AGCATAGTACCAATTGAAATAGCTTTCAATAATCTTCTCATTCTTAGGGTTCCCTTTTTGTTCCAATTGATCAGCACCAAACATTATGGAACAAGGTCTA
ACAAAACCAGCTCCGATAGACGTAAACACAAAAGATGAAAACAGAACAGCAAGCTGGAATGCTGTTGGTCCATTACAAACATGTTGCAACTGAGAACAGG
CCAAAGTCGTGAGTTGCGGAATCATTGATGTTAGCCAAAGAATAACCATTCCCTGTCGAAGAGGAATGTTAAGCCATAACAGATATGGTGGTTTTAAAAA
AACATGTTATTCCATTGAAATGGCACAGAAAACTCACAATAAAAGTGAAGATGGTTCCATAGGCAACAGCATTAAATCGACCCCAATAACAATCAGCAAT
AATCGCCCCAACAATAGCTAATCCACTTGAAAATGCGCCCCATATACCAATAATACTAGTACCCGTAGCAGCACTCATGTTATAATATGTCATCAAATAA
ATTATCAGATTTGTTTGTGAACCATAACTTGCTATCATTTCAAATGCTTCATTCACTGAAAACAACAAACAAGTGAAAAAACGGAATTATAATTAACACA
TTGCAAAACGATTCAAGTAAAGAAAATGAAAGAGTAAGAGTAATACTAATGATGAATGGCATGGTTCTAAGACCACCCTTTCTTGTGTTCACTTCAACTT
GTGATTCTGTGCCCATTTTCAGTTTCGTTCTCTGTTTCCTCTTCACTTCTCTTCTTGCCTCTAATATATGATTAACAGAGACAAAGAGACAGAGAAAAAA
ACACTAAAACCCATGCCCACATTTATCACCCTTAAATTTATTGCTACCAAAATATTAAATAAGAATCACGGATTCTCATTCAATATTCTGAAAGCATAGA
ATTTTCTTAATAAATATGTCAAATTTTATTATAACAAGTAAAATGAAACGAATGAAATATTTTAGATGTGATAATTGATAAATAATTAATGTTATCAATG
AATATGTAATAATTTAAAAATGGTCTAGTTTTGTTTTGTCTCACCTAAAAGAGGAAAATACAAAGTGACATGGAGTTAGTTATGTTTGATATTAATTATA
CTAATATTATCTTTTCTCAATTATTTGATTGATTATATTAAATATGTCGAAGGTCTTAAAATGAAAACAATATTTTTCTCCGACTTAACTTGTGTGACAT
GTTTTGAATTTTGAGATTTAAGCAAGTTTATTTTAAATCGTAATTTTTTCATATATTTTTTAAATAATTTAATTTGTTTATATTATGACTTATATATAAT
ATTGTTTACATATTATACATATATGTAAATTTTATTTTTAAAAATTAAACTTTCATACTAAACTTCGCTCAAATCTAAATTATTTAAAAATATCACATAA
ATTAAAATAAAAAAATACTTAATATAGTGAAATAAGAACAATCATAACAATGCACATTAATAATTGTAGCCAACCATGTCAACTCAATATCAATCACAAG
GCAATAGCTTATTTAATTCGAGTCATTTTTTTTTATCGGATATTGGAAGCACCATTTCAAGCCCCCCCATTTGACCATGAGTATCTTCTTTTTTTTTTTA
GTTAAGAAATTTCAAAATCTATGTTTGTTCATAAAACACATAATAAATAAACACGATTTTTAACTTAACGTCAACAAACAATTGTTTTTCAGTTTTAAAT
GTACACAAGTAGACACTTAAATTATTATTAAATTGAAAAAATAGATACAAATATCCTACATGACATGATACACATAGGATGTCATTTAGGATTAATGTGT
TCATTTTCTTAATTTTATACAAGTTTAAGTGTCTACTTGTGCATGTTCAAACTTTAAAGTCATATCTATGTATTATGCCTTTATAGAATATATTTTTTCT
AACTTCAACCATTATAATTATGTAGAACATATTACACGTAGGACATTATATAGATGTGAATTGTCACACAAGATGTA
The mutation and phenotype for selected feature: Solyc05g005940
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-10 (T0 ) | wt (47.8%) / -9 bp (49%) | Morphology : Boron-deficient phenotype; few seeds |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-10-5 (T1 ) | -9 bp/WT | Morphology : Boron-dificient phenotype; sterile |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-19 (T0 ) | 5 nucleotides substitution | |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-19-2 (T1 ) |
5 nucleotides substitution
Is tDNA present: No
Mutation sequence: TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGGTCGTCCTCATG CCCTTCTGTGATTGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACC CAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCG TAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGAT ATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Stunted growth; very branchy; smaller; Germination ~60% |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-2 (T0 ) | -1 bp/-1 bp | |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-2-4 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: ATTCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAA TATAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAG CCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAG TCCCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAG CCTCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCA TGCCCTTCGCTATTGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAAC CCAATTCCCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATC GTAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGA TATGTCTATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGAC CACATAGGAAGTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGA TCCATCAGGATTCAAATCTCTTTCAGGATCTTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Elongated internodes; rippled or wavy leaves |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-28 (T0 ) | -3 bp (47.2%) / -6 bp (49.4%) | |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-28-2 (T1 ) |
-6 bp/-6 bp
Is tDNA present: Yes
Mutation sequence: TCTTTCTCATCAGCCACATGAGGTGATCTACTCATCTCATCCTTCCCAGTATCATAAAACCGGCAGACAACTAGAAAATA TAGCAGGTTGAGAAAACTCATAGAAGCAAGCACCCAGAAATGGTAATCCAGGTGACCCTTATTAATGTTGCTCGAAAGCC AGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCTTCACTACTCACCAGAATACTTCCAGTCAAACTCGACACAGCAGTC CCAACGGTCTG0138-15TTGCCATGCTTTTCGGTAATAGAGTGTAGAAAAACTCAATCTGTCCAACTCCATGGGTAGCC TCAGCTATTCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGATCGTCTTCATG CCCTTCCGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTC CCATTCGGGTAACAGGACTTAGCCCTCTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCGTAGAAA GTAATCCAGATTGTTAAAGCAATAATCAAGAACACACTGAATGATGCTGCTGGTACTTCAAAGTGCGGGAAGATATGTCT ATCCATGGTTTTTGTTTGAAGTACAGCAAATGCAAATACATTCATGTCTACAAACATCATAAAACCAGTAGACCACATAG GAAGTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACATAGATTCCAAGGATCCGAAACTGATCCATCA GGATTCAAATCTCTTTCAGGATCTTCAATCATACAAGCTCTAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-33 (T0 ) | -7 bp/-7 bp | |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-51 (T0 ) | wt (25.7%) / -1 bp (31%) / -5 bp (33.3%) | |
| CRT0138 | RG-PtoR | CTG0138-14 | CGR0138-14 | CRT0138-62 (T0 ) | -1 bp (19.8%); -6 bp (20%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
