Name: Solyc05g005950
(Exon Intron)
Length: 2359
ATGCACACTCAAAGTTATCAACTCAATACAAGTTAAGTTCAGAGACATACTCATCCAGAATGTATGAAACTATATGAAGATTGTAAAGGTCTTAACATATAAGAACACTTTATTATCTAACTTAATTAACTGTCAAGAATGCAGTACAACATATCATCAAGATTCATGTAAGAGTCTATAATCACACTCTTCCTCATCAG
CCACATGAGGTAATTCATCATTCCCAGTCTCATAAAACCGGCAGACCCATAGAAAATATAACAAGTTGAGAAAACTCATTAAAGCAAGCAACCAGAAATG
GTAATCCAGGTGACCCTTATTAATGTTGCTAGAAAGCCAGCTCGTTTTACCTCCCCTTGATGTAAGCCAATCCTCACTACTCACCAGAATACTTCCAATC
AAACTCGACACAGCAGTTCCAACAGTGTACATAGCTGACGCGATGCTGGCCATGCTTTTCGGTAATAGAGTGTAGAAGAATTCAATCTGTCCAACTCCAT
GGGTAGCCTCAGCTATCCCGAGTAGTGCATACTGTGGCACGAACCACATAGCAGACATGTTTACTAAAGCATTTGGGTCGTCCTCATGCCCTTCTGTGAT
TGCCCTTTTTCGTCTTATGCTTTCTGTTATTGCTGACAATGCCATAGACATACAAGAGACTGTTAACCCAATTCCCATTCGGGTAACAGGACTTAGCCCT
CTTGGCCTTCCAGTATATTTTGATAGCAAAGGGACCAAAACACGATCATAGAAAGTAATCCAGATTGTTAAAGCAATAATCAAGAACACGCTGAATGATG
CTGCTGGTACTTCAAAGTGTGGCAAGATATGTCTATCCATTGTCTTTGTTTGAAGTACAGAAAATGCGAATACATTCATGTCTACAAACATCATAAAACC
GGCTGACCACATAGGAATTATTCTAATAAGAGCCTTGAGTGATTCAACTCGTTCCACACTACACAGATTCCATGGATTTGAAGCTGATCCATCAGCATTC
AAATCTCTTTCAGGATCTTCAATCATACAAGCTCTATTTAAGCACCTATATATATGTTCATCATACAATAATGTCTTATTCCAATGAACTTGTAATATAA
TAAACTCAGACAAAAGACGGAGACACTGACCTGAAGTCTTTTGATGGCGTTAAGACCTCTGTTTCATATGGCGAATGATAGTAGTCATCACAACCAGTCA
ATGAAAGAGGGGTTTTCCTTTTTCTAAAAGCAGCTGCACCTGCTTGGAACAATCCTAATAGCAAGTTGTTTGTATCGGGTTTCACTTTGATATAAAGAGA
AGAACCGATTAGGAACGTTGAGACAGACAAAACCATGAGTATAACAGGGATACCAAAGCCAATTTGCCAACCATAACGATCTTGAATATAAATAATAACG
GTAACTGCAATCATAGTTGCAACCCCTGTGCTAGCATAGTACCAATTGAAATAGCTCTCAACAATCTTCTTGTTTTCAGGGTTGCCTTTTTGTTCCAATT
GATCAGCACCAAACATTATAGAACAAGGTCTAACAAAACCAGCTCCGATAGACGTAAACACAAAAGATGTAAACAGAACAGCAAGCTGGAATGCTGTTGG
TCCATTACAAACATGTTGAAAATGAGAACATGCCAAAGTTGTGAGTTGCGGAATCATTGATGTAAGCCAGAGAATAACCATTCCCTGTCCAAGAAGAATG
TTAAGCCATAACAGATATGCTGGTTCAAATTTTAAAAAAAATGTTGTTAAATTAGCACAACTCACAATAAAAGTGAAGATGGTTCCATAGGCAACAGCAT
TATATCGACCCCAATAACAATCAGCAATAATCGCCCCAACAATAGCCAATCCACTTGAAAGTGCACCCCATATACCAATAATACTAGTGCCAGTAGCAGC
ACTCATGTTATAATATGTCATCAAATAAATTATCATGTTTGTTTGTGAACCATAACTTGCTATCCTTTCAAATGATTCATTCACTGAAAACAATAGCAAC
AACAATAACAAAAAATTGATATTAGAATTAACACATTGCAAAACAATTTGAAGTAAAAGAGTAGAGTGAGAGTAGTACAAATGATGAATGGCATGGTCCT
AAGACCACCCTTTCTTGTGTTTATTTCAGCTTGTGATTCTGTGCCCATTTCCACTGCTATTTTCTGTTTCCTCTTCACTTCTCTTCTTTGCTTTAATATA
TGATTAGCAGACACAAAAAGAGAGAGAGAAAAAAACACTAAACCCCAGGACCATGTTTATGGCCTTAAATTTATTGCTTCCAAAATATTGACTGAGAACG
AGACGTGACAGAGTTACCCTGTAATCCTCTGTCAAACGCATTATAAATTTTGAGATTCA
The mutation and phenotype for selected feature: Solyc05g005950
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1 (T0 ) | -4 bp (46.5%); -15 bp (45%) | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1-1 (T1 ) | -15 bp/-15 bp | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1-2 (T1 ) | -4 bp/-15 bp | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1-8 (T1 ) | -4 /-15 bp | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1-8-4 (T2 ) | -4 bp | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-1-8-8 (T2 ) | -4 bp | |
| CRT0127 | RG-prf3 | CTG0138-16 | CGR0138-16 | CRT0127-2 (T0 ) | TIDE didn't work | |
| CRT0138 | RG-PtoR | CTG0138-15 | CGR0138-15 | CRT0138-55 (T0 ) | WT(78.1%) / +1 bp (6.6%) / -1 bp (2.6%) | |
| CRT0138 | RG-PtoR | CTG0138-16 | CGR0138-16 | CRT0138-51 (T0 ) | wt (17.1%) / -1 bp (22.7%) / -3 bp (22.1%) / +1 bp (2.5%) | |
| CRT0138 | RG-PtoR | CTG0138-16 | CGR0138-16 | CRT0138-6 (T0 ) | -3 bp (38.2%) / -6 bp (50.9 %) | |
| CRT0138 | RG-PtoR | CTG0138-16 | CGR0138-16 | CRT0138-6-11 (T1 ) |
-3 bp/-3 bp
Is tDNA present: No
Mutation sequence: GACCATGCCATTCATCATTTGTACTACTCTCACTCTACTCTTTTACTTCAAATTGTTTTGCAATGTGTTAATTCTAATAT CAATTTTTTGTTATTGTTGTTGCTATTGTTTTCAGTGAATGAATCATTTGAAAGGATAGCAAGTTATGGTTCACAAACAA ACATGATAATTTATTTGATGACATATTATAACATGAGTGCTGCTACTGGCACTAGTATTGGTATATGGGGTGCACTTTCA AGTGGATTGGCTATTGTTGGGGCGATTATTGCTGATTGTTATTGGGGTCGATATAATGCTGTTGCCTATGGAACCATCTT CACTTTTATTGTGAGTTGTGCTAATTTAACAACATTTTTTTTAAAATTTGAACCAGCATATCTGTTATGGCTTAACATTC TTCTTGGACAGGGAATGGTTATTCTCTGGCTTACATCAATGATTCCGCAACTCACAACTTTGGCATGTTCTCATTTTCAA CATGTTTGTAATGGACCAACAGCATTCCAGCTTGCTGTTCTGTTTACATCTTTTGTGTTTACGTCTATCGGAGCTGGTTT TGTTAGACCTTGTTCTATAATGTTTGGTGCTGATCAATTGGAACAAAAAGGCAACCCTGAAAACAAGAAGATTGTTGAGA GCTATTTCAATTGGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
