Name: Solyc05g009500
(Exon Intron)
Length: 4595
ACGTTCATACACGTATGAATAATAAACTCCAGTCATTTACCTGAAGTTCATGCACATATAACTTAAATTCGTAATACAAATAGTCAAAGTTTTACTATTCCATCTATTAATAATTATTATTACATAATACATTAAACTATTCATAACCATATTCTTTTCTTTCCTCATAACCTAAAAAAACTATTACATAATACTATAAA
ACTAAAGTTGCAAAATACAAACTAAACATTGTTTACAAGTTCAATCCCCTCTTTTCTAGTCTCCAACTCCTCTGTTTTGTGCATCTGAATAGGCTTAAAA
GTATATAGTCTTGCACAAAACACATAGTAAATCAAATTAACCACTTGTAAAATCGTAATTAACCAGTAAAAATACTCTAATCTCCCCTCATTCAAGTTAT
TATCAGGCAGCCAATTGGTAAATTTATGAACTAACGAAACCAGAAATGTACTCAAATAGTTCCCAGCTGAAATCGATGTCCAAAATAACGCGGTTGCTGT
ACTTCTCATACTCTCTGGAGCTTGATCATAGAAAAATTCAAGATGTCCAATTGACATAAATGCCTCAGCTATACCATGTAAACAATACTGTGGCACTAGC
CAAAATACTGAAATTGGAACTAGTGATTTAGGTTTATCGACTAGTCCATAAGTCGATGCAACATTTTTTCGTTTAACTTCAATAAATCCAGCTACTAATG
TAGCTAGAACAGAAATGAAGAATCCAATTCCCATTCTACTTAGGAATGAGATTCCTCGTTCGAGTCCAGTGAACTTACGTGCAATAGGTACAAATACGCG
GTCATAGAAGGCAATGGTGCATAGCATTGTCGTTAATGTGAAGACAGTCATCGAGGCAGCTGGGATTTCGAAGGAGTTTAGTAGGTGTCTGTTCATTGTT
TTGGCTTGTTGGACTGAGAATGTGGATTGTTGAGCGTATGCTGTGATCAGGATGATCCCTGATGCCCATATTGGACCCATTCTTATGATCGATTTGAGTT
CTTCTACGCGATGAACTGTGTTTAGCCTCCATAGATTCGGAGATTTAGGGTTGTCCTCCTCTGTTACTATTGCTGCTCTGTCCAACAATCTGAAAATTAA
ACTTTTAAATGAGATGGTCACGTAATAAACGTCTTAGTTAACTCACTCAATAGTCAAAGATATTAGTAACATGTGAGTATGATGGTTGTTTGAAGATTTT
TCAAAGACATTAGCATGTTGCATGCCTTTCTTAATCAAGTCTACATGATAATGAAATACGTTTAGTCTAACATTAGGGGTTAGGATCTATCTTGTCGGAT
CCTCGAAAAAAACACTGTCAAGATATGACATCCAGTCTAACGTAACTCAACCCCAAAAGCTAGCTCATGAGGGGAGGTTTGTCCAAGTCTATATAAGGAG
ACTGAATTTTCATCCCTCGTCCAATGTGGGACTTTTTAATGAATAAGTACCTGTCGACATTATTAAAGAGTCCGAGCAGGAGAGACTAACTTAAAAGTCA
TCCAAATTTTGACTAGATTTTTTATACATTTGGGTATAAATTAACTCAAATTTGATAGTATAGTTCCAAAAGATACAACTTTTGGCAGTATTAATCAACT
CGTTTTTTGATTTTTAATGGCTTCAGTTCTTGCAACATTATTGAAGCTATGAAAATAATAAAATCAAATTAAATTGGAAATGTGAATAAAATTCAATGAA
TCTTGATTTTGATGGGGTGTACAGAGACATGAAAATAAAGACAAATAACAGGACAGACAACATTTATTCACATAATGTTTTTGGCCACACAATGAATAGA
CATACATGTACAAATTGTTTTGAGATAAAAATTTAATAGCAAGACATTTGATTTATCATAAACAAGAGATAAAAACTTATCTCCTTAAAAAAGAAAATTT
CTAAATTTAATTAAAGTTAGCATACAGAATATTCTAATATCTTGAGCTCTTAATTTTTTACTGTTATGTGTAATTATATCAATTATTCACACAAGATTCC
TCTCCATTATTGTCTTCAATTTAACAAAAAAAAGTTGCAAAACTTATATGTGATGGATTTTCTAAACAAACAAAAAATAATTAACTTATGCACACCGACA
CTATAAAAGTAATTTATTCTATAAGTATAATTTAATATATTATAACAAGCTACTACTTCCGCTTATTATGAAAAGTTATATATGCACTATCAGTGTATAT
AACACACTACCAAATATTATTTTATCAATTTTATCGCCATAATCTTTAATGACCATATAAAGAGTTACCTATTGTTGTAAGCTATAGTTCTCGTACTCTT
CAAAAAGGTTGATATACTCATGTCAAATTCTCCAAAATACATTCAAAGGATTCAACACGCATGTTACAACATTTTAAAAGAATATGAGCAGCATATATTG
CATGCCAATCGTAATTAATAGTCTAAAAGATACACACAATCAGTCAGTGCACGAAACTTAAATTCGGAAGAAGAAAATGACTTACTTCATTTGCTTAGTG
TGGACAAGCTTGCCAGCAGTAGAAATATCAGCATCAAGTTCTTGATTTTGATAAAGCAAATTAGTATCATTTACCATATCCAATTTCCTCTTTTTGTAGG
CAGCAACACAAACTTGCACTAGCCTAGTAAAAGGGCTACCAGCAGGATCCAAATTTCTATACAAAGGGTATCCAAATATAAAAACAACAATTGATATAAG
CATAGCAATTGTTGGGACACCAAATCCAACACCCCATCCAATATTGTCTTGAATATAAACAATTACTGTGACAGCCACAAGCATTGAAAATCCCATACAA
AAATAATACCAATTAAAGAATTTCCATGTTTGTGTTTTTTGATTTGGATCATTTTCATCAAATTGGTCAGCCCCAAATGCAACAACACATGGCCTAATTC
CACCTGACCCAAAGGCTGTTAAGAGTAATGACACATATAAAATGGCTATTTGACTTGAATTTGCTTCTTTGCAATATTCATCATCTTTGCATGATGGTGG
CCTTAGTTGTGGTAGTATTGCTGATAATGTTAATATGATCATGCCCTGAAATTAAAATCAAAAGGGGGTTCGGGATTTTAGTCCTTTCGAGTTATTACGT
TCTAAAGTCAACAATTTGAATTCCAGTATACTATACTCTAACTTCGCTCCTGAATTTTGAACTATGATATGTTTGTTGTACATGTTATATAATACTACAC
TGACGATAGTACTATAAAATAATATCTTTAAACTATCATATCACTCAGATGATTATTATGTGTAACTTTCCATTAAAGTAATGATTGGAAACGAAAAAAA
TTAAATAAAGTTGATTACTTATAAAAATAAATTATTTATGTTGTCAACATATATATGTAATATTTTATTTAGCTTGAGTTTGAAAAGACAAAGTTAACTA
TTCACATTTAATATATTTACTGTAAATTCTTTATGAAACCAAACTACCAAGATTTTGCCTATCCAAATTAAGTCATTCTCCATTTATATCCTATCCATTA
CAGGCTTATCAGAAAAAGATAAATAAGCCATTTAATAAGTATTCAAGGAACTAGTAAAAATATTACAACCCCTCACACTAATAATTTGAGTTTTACTTGA
TACAACTATTTTATGCATACTATGTAGTATCAAATTATATGTCATATTTATTTTAACATCTAACCTAAAAAAATTAAAAGAAGACAACTTGCATACATAA
TACAAATACAATGGTCTAAACAAAAGTTAGTAAATTCGTTTAAACTCGTAACTGATACTCTTCCTCTGATCCGTCTTTCATGTCAGCGTATAGAACTTAA
ACTCGAGAAAAAAAATGTGTGAGAGAGAGACTTACAATTTGGTAGATGATAGAAGCAACAGTTATAGTCCAAAATCTTCCAGCAAAAGTATCAGCAATAA
AAGCTCCAAACAATGGTGTCAAACTTGCAGTGCCACCAAAATTTGTTAGTGTATTTGCTGCTTTTGTTAATGGAAGATGGAGTTCATTTGTCAAGTAAAT
GACCATATTTGTACCAAACCCAACCACTGCCAATTTCTCACATATCTCATTTGCTACACACAAAAAAAGCATATTAAGTTCAATTTCAACTTAGTATTCA
AGAAAGAATAGCGTTGGAGCAACGATAAAATTATTTTTCTATGATTTATATGTTACGAGTTCGTTTTCGTGGGGTGCAACCCTTACACATTATGTTTTGT
ACACCAGATTTTCTTTTATTATTCAAGAAACTATACTTAAGATTCACAAAAGAATTGAGTAAAAAGTTGGAAAACTAAAGCCACGAATTTGAATTGTGAA
AAAAGTTATCAATGTTTGTATTAGAATTATGTTATATATATCCCACCTCTTGAGGTGCGATCCTTTCTTGACTTTCAGAACCTGCTACTATAAAATACTT
TGTACACCAAAATGTTTTTTTTATTCAACAAATTATGTGTAATTTCAAGATTCTCAAAAAAAAAAAAGCGAAAAGTTGAAAAAAAAAATAAACTAACCAA
ATATGAAGGGCATAGTGATCATTCCACCCTTCATCCTTCTAGAGGAGTTTTCCTTCTTATTCATCACTTCCATATTTGTATGTAATTGTGTGCTC
The mutation and phenotype for selected feature: Solyc05g009500
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-1 (T0 ) | +1 bp (29.1%); -4 bp (27%); -7 bp (33.6%) | |
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-2 (T0 ) | -4 bp (49.4%); -5 bp (47.5%) | |
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-2-1 (T1 ) | -4 bp/-4 bp | |
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-2-10 (T1 ) | -5 bp/-5 bp | |
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-2-6 (T1 ) | -4 bp/-5 bp | |
| CRT0128 | RG-prf3 | CTG0138-18 | CGR0138-18 | CRT0128-4 (T0 ) | hetero (large deletion >70 bp) | |
| CRT0138 | RG-PtoR | CTG0138-18 | CGR0138-18 | CRT0138-30 (T0 ) | +1 bp/+1 bp | |
| CRT0138 | RG-PtoR | CTG0138-18 | CGR0138-18 | CRT0138-33 (T0 ) | didn't work with TIDE | |
| CRT0138 | RG-PtoR | CTG0138-18 | CGR0138-18 | CRT0138-6 (T0 ) | didn't work with TIDE | |
| CRT0138 | RG-PtoR | CTG0138-18 | CGR0138-18 | CRT0138-6-11 (T1 ) |
-64 bp/-64 bp
Is tDNA present: No
Mutation sequence: CTTTGACTATTGAGTGAGTTAACTAAGACGTTTATTACGTGACCATCTCATTTAAACGTTTAATTTTCAGATTGTTGGAC AGAGCAGCAATAGTAACAGAGGAGGACAACCCTAAATCTCCGAATCTATGGAGGCTAAACACAGTTCATCGCGTAGAAGA ACTCAACAATCCACATTCTCAGTCCAACAAGCCAAAACAATGAACAGACACCTACTAAACTCCTTCGAAATCCCAGCTGC CTCGATGACTGTCTTCACATTAACGACAATGCTATGCACCATTGCCTTCTATGACCGCGTATTTGTACCTATTGCACGTA AGTTCACTGGACTCGAACGAGGAATCTCATTCCTAAGTAGAATGGGAATTGGATTCTTCATATCTGTTCTAGCTACATTA GTAGCTGGATTTATTGAAGTTAAACGAAAAAATGTTGCATCGACTTATGGACTAGTCGATAAACCTAAATCACTAGTTCC AATTTCAGTATTTTGGCTAGTGCCACAGTATTGTTTACATGGTATAGCTGAGGCATTTATGTCAATTGGACATCTTGAAT TTTTCTATGATCAAGCTCCAGAGAGTATGAGAAGTACAGCAACCGCGTTATTTTGGACATCGATTTCAGCTGGGAACTAT TTGAGTACATTTCTGGTTTCGTTAGTTCATAAATTTACCAATTGGCTGCCTGATAATAACTTGAATG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
