Plant Crispr Database

Feature Information

Feature ID: Solyc06g005230
Feature Type: gene
Name: Mal2
Description: Malectin-binding domain protein 2; Pto-like domain
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc06g005230

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation
CRT0003	RG-prf3	CTG0003-2	CGR0003-2	CRT0003-1 (T0 )	-200 bp/-200 bp Is tDNA present: Yes Mutation sequence: TTGTCATTAAATAGTTTATTTTTTGTACTGTTGTTGGGTTTTTACTCAAACTTCTAGTTATAAATTCCCTATTAGTAAAC TTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGATATGAGTTTAGCAACTTTCTCTGTT TCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGTTAAGGAGTTTTCTGTTAACGTAAC GACGGGTGATCTTGTCATCACTTTTTCTCCTTTGAGTGATTCATTTGCTTATGTAAATGCTTTGGAAGTTGTCTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 370 CCACTTCATCGCCCATTCAGCTAAATTAACCATCTCCCTTGGAAG-AGAT 418 \|.\|\|\|\|\| \|\| \|\|.\|\|\|.\|.\|.\| \|\|\| \|.\|\| MutationRev 6 CAACTTC----------CA--------AAGCATTTACAT---AAGCAAAT 34 Wildtype 419 GGATCGATTACAGGCCTAGCACAAAG-------AACTTCA---------- 451 \|.\|\|\|..\|.\|.\|\|\| \|\| \|.\|\|\|\| \|\|..\|\|\| MutationRev 35 GAATCACTCAAAGG---AG-AAAAAGTGATGACAAGATCACCCGTCGTTA 80 Wildtype 452 ----AACAGAACAACACCAAAAGAGTAAACATCACTTTTCTCAGTCAATT 497 \|\|\|\|\|\|\| \|\|\|.\|\|..\|\|.\|\| \|.\|\|\|\|\|..\|\|\|\|...\| MutationRev 81 CGTTAACAGAA-AACTCCTTAACAG-ATACATCTTTTTTAGGA------- 121 Wildtype 498 GCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGAAGCTCCCTTTCACT 547 \|\|.\|\|\|\| \|\|\| \|.\|\|\| \|\|\|.\|..\|\|\| MutationRev 122 --------CTAAAAT-TTC-----CAAGG-------AGCACATTTT---- 146 Wildtype 548 GCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCCTGCCTTAGAAAGTCC 597 \|\| \|\|\|\| \|\|\|\|...\|\| \|\|\|\|\|\| MutationRev 147 ---GT-------TGAG-TTGAAACAG---------------AGAAAG--- 167 Wildtype 598 GAAATCAGCTACTTTTGCCA---TCATATTCTCATCAAGCAATATGTTTG 644 \|\|\|\|.\| \|\|\|\|\| \|\|\|\|.\| MutationRev 168 --------------TTGCTAAACTCATA---TCATAA------------- 187 Wildtype 645 CTGATTTG-ACATCACGATGTATAACTGCTTTGGCGTAGCTAGTATGAAG 693 \|\|\|\|.\| \|\|\|..\|.\|..\|.\|.\|\| \|\|\|\| MutationRev 188 --GATTGGTACACAAAGGGGGAAAA-------------------ATGA-- 214 Wildtype 694 GTAGTGTAGACCTCTGGCTGCCCCAATACATATCTCCAGCCTCTGTTTCC 743 \|\|\|\| \|\|.\| \|\|\|\|\|.\|.\|\| \|\|\| .\|\|\|\|.\| MutationRev 215 -------AGAC----GGAT---CCAATGCCTA---CCA-----AGTTTAC 242 Wildtype 744 AGCTCATACTAGGCA---TATCCGAACCATACAGATGGCTCTTAAGGGTC 790 \|\|.\|\|\|\|.\| \|\|\| \|\|\|.\|.\| MutationRev 243 ------TAATAGGGAATTTAT---AACTAGA------------------- 264 Wildtype 791 CCATTTTCCATGTACTCGTACACGAGAATCATCTCATTCTTTTCATCACA 840 \|.\|\|\| \|\|\|.\|..\|.\|.\|\| \|..\|\|\| MutationRev 265 --AGTTT----------------GAGTAAAAACCCA--------ACAACA 288 Wildtype 841 GTATCCAATCAATGAAACAA---GATGACGA 868 \|\|\| \|\|\|..\|\|\| \|\|\|\|.\| .\|\|\|\|\|.\| MutationRev 289 GTA--CAAAAAAT-AAACTATTTAATGACAA 316
CRT0003	RG-prf3	CTG0003-2	CGR0003-2	CRT0003-1-49 (T1 )	-200 bp/-200 bp Is tDNA present: Yes Mutation sequence: TTGTCATTAAATAGTTTATTTTTTGTACTGTTGTTGGGTTTTTACTCAAACTTCTAGTTATAAATTCCCTATTAGTAAAC TTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGATATGAGTTTAGCAACTTTCTCTGTT TCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGTTAAGGAGTTTTCTGTTAACGTAAC GACGGGTGATCTTGTCATCACTTTTTCTCCTTTGAGTGATTCATTTGCTTATGTAAATGCTTTGGAAGTTGTCTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 370 CCACTTCATCGCCCATTCAGCTAAATTAACCATCTCCCTTGGAAG-AGAT 418 \|.\|\|\|\|\| \|\| \|\|.\|\|\|.\|.\|.\| \|\|\| \|.\|\| MutationRev 6 CAACTTC----------CA--------AAGCATTTACAT---AAGCAAAT 34 Wildtype 419 GGATCGATTACAGGCCTAGCACAAAG-------AACTTCA---------- 451 \|.\|\|\|..\|.\|.\|\|\| \|\| \|.\|\|\|\| \|\|..\|\|\| MutationRev 35 GAATCACTCAAAGG---AG-AAAAAGTGATGACAAGATCACCCGTCGTTA 80 Wildtype 452 ----AACAGAACAACACCAAAAGAGTAAACATCACTTTTCTCAGTCAATT 497 \|\|\|\|\|\|\| \|\|\|.\|\|..\|\|.\|\| \|.\|\|\|\|\|..\|\|\|\|...\| MutationRev 81 CGTTAACAGAA-AACTCCTTAACAG-ATACATCTTTTTTAGGA------- 121 Wildtype 498 GCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGAAGCTCCCTTTCACT 547 \|\|.\|\|\|\| \|\|\| \|.\|\|\| \|\|\|.\|..\|\|\| MutationRev 122 --------CTAAAAT-TTC-----CAAGG-------AGCACATTTT---- 146 Wildtype 548 GCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCCTGCCTTAGAAAGTCC 597 \|\| \|\|\|\| \|\|\|\|...\|\| \|\|\|\|\|\| MutationRev 147 ---GT-------TGAG-TTGAAACAG---------------AGAAAG--- 167 Wildtype 598 GAAATCAGCTACTTTTGCCA---TCATATTCTCATCAAGCAATATGTTTG 644 \|\|\|\|.\| \|\|\|\|\| \|\|\|\|.\| MutationRev 168 --------------TTGCTAAACTCATA---TCATAA------------- 187 Wildtype 645 CTGATTTG-ACATCACGATGTATAACTGCTTTGGCGTAGCTAGTATGAAG 693 \|\|\|\|.\| \|\|\|..\|.\|..\|.\|.\|\| \|\|\|\| MutationRev 188 --GATTGGTACACAAAGGGGGAAAA-------------------ATGA-- 214 Wildtype 694 GTAGTGTAGACCTCTGGCTGCCCCAATACATATCTCCAGCCTCTGTTTCC 743 \|\|\|\| \|\|.\| \|\|\|\|\|.\|.\|\| \|\|\| .\|\|\|\|.\| MutationRev 215 -------AGAC----GGAT---CCAATGCCTA---CCA-----AGTTTAC 242 Wildtype 744 AGCTCATACTAGGCA---TATCCGAACCATACAGATGGCTCTTAAGGGTC 790 \|\|.\|\|\|\|.\| \|\|\| \|\|\|.\|.\| MutationRev 243 ------TAATAGGGAATTTAT---AACTAGA------------------- 264 Wildtype 791 CCATTTTCCATGTACTCGTACACGAGAATCATCTCATTCTTTTCATCACA 840 \|.\|\|\| \|\|\|.\|..\|.\|.\|\| \|..\|\|\| MutationRev 265 --AGTTT----------------GAGTAAAAACCCA--------ACAACA 288 Wildtype 841 GTATCCAATCAATGAAACAA---GATGACGA 868 \|\|\| \|\|\|..\|\|\| \|\|\|\|.\| .\|\|\|\|\|.\| MutationRev 289 GTA--CAAAAAAT-AAACTATTTAATGACAA 316
CRT0003	RG-prf3	CTG0003-2	CGR0003-2	CRT0003-1-49-121 (T2 )	-200 bp/-200 bp Is tDNA present: Yes Mutation sequence: TTGTCATTAAATAGTTTATTTTTTGTACTGTTGTTGGGTTTTTACTCAAACTTCTAGTTATAAATTCCCTATTAGTAAAC TTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGATATGAGTTTAGCAACTTTCTCTGTT TCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGTTAAGGAGTTTTCTGTTAACGTAAC GACGGGTGATCTTGTCATCACTTTTTCTCCTTTGAGTGATTCATTTGCTTATGTAAATGCTTTGGAAGTTGTCTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 370 CCACTTCATCGCCCATTCAGCTAAATTAACCATCTCCCTTGGAAG-AGAT 418 \|.\|\|\|\|\| \|\| \|\|.\|\|\|.\|.\|.\| \|\|\| \|.\|\| MutationRev 6 CAACTTC----------CA--------AAGCATTTACAT---AAGCAAAT 34 Wildtype 419 GGATCGATTACAGGCCTAGCACAAAG-------AACTTCA---------- 451 \|.\|\|\|..\|.\|.\|\|\| \|\| \|.\|\|\|\| \|\|..\|\|\| MutationRev 35 GAATCACTCAAAGG---AG-AAAAAGTGATGACAAGATCACCCGTCGTTA 80 Wildtype 452 ----AACAGAACAACACCAAAAGAGTAAACATCACTTTTCTCAGTCAATT 497 \|\|\|\|\|\|\| \|\|\|.\|\|..\|\|.\|\| \|.\|\|\|\|\|..\|\|\|\|...\| MutationRev 81 CGTTAACAGAA-AACTCCTTAACAG-ATACATCTTTTTTAGGA------- 121 Wildtype 498 GCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGAAGCTCCCTTTCACT 547 \|\|.\|\|\|\| \|\|\| \|.\|\|\| \|\|\|.\|..\|\|\| MutationRev 122 --------CTAAAAT-TTC-----CAAGG-------AGCACATTTT---- 146 Wildtype 548 GCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCCTGCCTTAGAAAGTCC 597 \|\| \|\|\|\| \|\|\|\|...\|\| \|\|\|\|\|\| MutationRev 147 ---GT-------TGAG-TTGAAACAG---------------AGAAAG--- 167 Wildtype 598 GAAATCAGCTACTTTTGCCA---TCATATTCTCATCAAGCAATATGTTTG 644 \|\|\|\|.\| \|\|\|\|\| \|\|\|\|.\| MutationRev 168 --------------TTGCTAAACTCATA---TCATAA------------- 187 Wildtype 645 CTGATTTG-ACATCACGATGTATAACTGCTTTGGCGTAGCTAGTATGAAG 693 \|\|\|\|.\| \|\|\|..\|.\|..\|.\|.\|\| \|\|\|\| MutationRev 188 --GATTGGTACACAAAGGGGGAAAA-------------------ATGA-- 214 Wildtype 694 GTAGTGTAGACCTCTGGCTGCCCCAATACATATCTCCAGCCTCTGTTTCC 743 \|\|\|\| \|\|.\| \|\|\|\|\|.\|.\|\| \|\|\| .\|\|\|\|.\| MutationRev 215 -------AGAC----GGAT---CCAATGCCTA---CCA-----AGTTTAC 242 Wildtype 744 AGCTCATACTAGGCA---TATCCGAACCATACAGATGGCTCTTAAGGGTC 790 \|\|.\|\|\|\|.\| \|\|\| \|\|\|.\|.\| MutationRev 243 ------TAATAGGGAATTTAT---AACTAGA------------------- 264 Wildtype 791 CCATTTTCCATGTACTCGTACACGAGAATCATCTCATTCTTTTCATCACA 840 \|.\|\|\| \|\|\|.\|..\|.\|.\|\| \|..\|\|\| MutationRev 265 --AGTTT----------------GAGTAAAAACCCA--------ACAACA 288 Wildtype 841 GTATCCAATCAATGAAACAA---GATGACGA 868 \|\|\| \|\|\|..\|\|\| \|\|\|\|.\| .\|\|\|\|\|.\| MutationRev 289 GTA--CAAAAAAT-AAACTATTTAATGACAA 316
CRT0004	TA537	CTG0003-2	CGR0003-2	CRT0004-5 (T0 )	heterozygous
CRT0004	TA537	CTG0003-2	CGR0003-2	CRT0004-5-16 (T1 )	-6/WT
CRT0004	TA537	CTG0003-2	CGR0003-2	CRT0004-5-6 (T1 )	-4 bp/-4 bp Is tDNA present: Yes Mutation sequence: AGTTTATTTTTTGTACTGTTGTTGGGTTTTTTCTGTGTTTGTTCTGGATTTGATCCAGTGGATGAGTACTTAATAGACTG TGGATCTTCAAAAGATACTAATTTTGGTAATAGGGTTTTTGTGTCTGATAAATCTCTGGTTCTAAGTTTCTGTCAGTTTC TCAAGATATATTAGCTGATAATACAAAGTCTAGTGATTCACCCATTTATCAGACTGCTAGAGTTTTTACTCAAACTTCTA GTTATAAATTCCCTATTAGTAAACTTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGAT ATGAGTTTAGCAACTTTCTCTGTTTCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGT TAAGGAGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 403 CTCCCTTGGAAGAGATGGATCGATTACAGGCCTAGCA----CAAAGA--- 445 \|\|\|\|.\| \|\|.\|\|\|\|..\|\|\|..\|\|..\|\|\|.\|\|\|..\| \|\|\|.\|\| MutationRev 2 CTCCTT---AACAGATACATCTTTTTTAGGACTAAAATTTCCAAGGAGCA 48 Wildtype 446 ----------ACTTCAAACAGAACAACACCAAAAG--AGTAAACATCACT 483 \|.\|\|.\|\|\|\|\|\|\| .\|\|\|\| ..\|\|\|\|\| \|\|\| MutationRev 49 CATTTTGTTGAGTTGAAACAGA--------GAAAGTTGCTAAAC-TCA-- 87 Wildtype 484 TTTCTCAGTCAATTGCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGA 533 \|.\|\|\| .\|\|.\|\|\| \|\|\|\|\|.\|.\| MutationRev 88 --TATCA------------------TAAGATT---------GGTACACAA 108 Wildtype 534 AGCTCCCTTTCACTGCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCC- 582 \|\| \|.\|..\|\|.\|\|\|\| \|\| .\|\|\|.\|\|\| MutationRev 109 AG---------------GGGGAAAAATGA---------AG-ACGGATCCA 133 Wildtype 583 -TGCCTTAGAAAGTCCGAAATCAGCTACTTTTGCCATCATATTCTCATCA 631 \|\|\|\| \|\|..\|\|\|\| .\|\|\|\| MutationRev 134 ATGCC-TACCAAGT----------TTACT--------------------- 151 Wildtype 632 AGCAATATGTTTGCTGATTTGACATCACGATGTATAACTGCTTTGGCGTA 681 \|\|\|\|.\| \|\| \|\|.\|\|\|\|\|\| \|\| MutationRev 152 ---AATAGG-----------GA-------ATTTATAAC----------TA 170 Wildtype 682 GCTAGTATGAAGGTAGTGTAGACCTCT----GGCTGCCCCAATACAT--- 724 \| .\|\|\|.\|\|\| \|\|\| \|.\|.\|\|\|\| \|.\|\|\| \|\|\|.\|\| MutationRev 171 G-AAGTTTGA--GTA----AAAACTCTAGCAGTCTG-----ATAAATGGG 208 Wildtype 725 ---ATCTCCAGCCTCTG--TTTCCAGCTCATACTAGGCATATC---CGAA 766 \|\|\|.\|.\|\|.\|\|.\|\| \|\|..\|\|\|\|\|.\|\| \|\|\|\|\| .\|\|\| MutationRev 209 TGAATCACTAGACTTTGTATTATCAGCTAAT-------ATATCTTGAGAA 251 Wildtype 767 CCATACAGATGGCTCTTAAGGGTCCCATTTTCCATGTACTCGTACACGAG 816 .\|..\|\|\|\|\| ..\|\|\|\| \|.\|\|..\|\|\| MutationRev 252 ACTGACAGA---AACTTA-----------------------GAACCAGAG 275 Wildtype 817 AATCATCTCATTCTTTTCATCACAGTATCCAATCAATGAAACAAGATGAC 866 \| \|\|.\|\|\|\| \|\|.\|\|\|\|.\|...\| MutationRev 276 A--------------TTTATCA---------------GACACAAAAACCC 296 Wildtype 867 GATGACGGAACTGAGAGAGCATCTCTATTTCAGTTTGGAACTCTCTAAGA 916 .\|\|.\|\|..\|\|.\|.\|\| \|\|\|.\| \|\|\|\| \|\|\|\| MutationRev 297 TATTACCAAAATTAG----------TATCT---TTTG---------AAGA 324 Wildtype 917 CCTTGTTGGGAGCTAGGATTACCC---CTCTTAATAGCTACGTTCGTTCC 963 \|.\|.\| \|\|.\|\|\|\| \|\|.\| MutationRev 325 -------------------TCCACAGTCTATTAA-----------GTAC- 343 Wildtype 964 ATCATTTAAAACTCCCTTGAATACCTTACCAAAGCCACCTATCCCAATGA 1013 \|\|\| \|\|\|..\|\|.\|\| \|\|\|\|.\|\|\| \|\| MutationRev 344 -TCA--------TCCACTGGAT-------CAAATCCA-----------GA 366 Wildtype 1014 CCAAACTCTCATCAAACTTTTTGGTTGCTGCTAGCATGGCTGCAAAAGGA 1063 .\|\|\|\|\|.\|..\|..\|\|\|\| \|.\|.\|\|....\|.\|\|\|\|\| \| MutationRev 367 ACAAACACAGAAAAAAC-------------CCAACAACAGTACAAAA--A 401 Wildtype 1064 ATGCGATAACT 1074 \|\| .\|\|\|\| MutationRev 402 AT----AAACT 408
CRT0004	TA537	CTG0003-2	CGR0003-2	CRT0004-6 (T0 )	heterozygous
CRT0004	TA537	CTG0003-2	CGR0003-2	CRT0004-6-38 (T1 )	-4 bp/-4 bp Is tDNA present: No Mutation sequence: AGTTTATTTTTTGTACTGTTGTTGGGTTTTTTCTGTGTTTGTTCTGGATTTGATCCAGTGGATGAGTACTTAATAGACTG TGGATCTTCAAAAGATACTAATTTTGGTAATAGGGTTTTTGTGTCTGATAAATCTCTGGTTCTAAGTTTCTGTCAGTTTC TCAAGATATATTAGCTGATAATACAAAGTCTAGTGATTCACCCATTTATCAGACTGCTAGAGTTTTTACTCAAACTTCTA GTTATAAATTCCCTATTAGTAAACTTGGTAGGCATTGGATCCGTCTTCATTTTTCCCCCTTTGTGTACCAATCTTATGAT ATGAGTTTAGCAACTTTCTCTGTTTCAACTCAACAAAATGTGCTCCTTGGAAATTTTAGTCCTAAAAAAGATGTATCTGT TAAGGAGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 403 CTCCCTTGGAAGAGATGGATCGATTACAGGCCTAGCA----CAAAGA--- 445 \|\|\|\|.\| \|\|.\|\|\|\|..\|\|\|..\|\|..\|\|\|.\|\|\|..\| \|\|\|.\|\| MutationRev 2 CTCCTT---AACAGATACATCTTTTTTAGGACTAAAATTTCCAAGGAGCA 48 Wildtype 446 ----------ACTTCAAACAGAACAACACCAAAAG--AGTAAACATCACT 483 \|.\|\|.\|\|\|\|\|\|\| .\|\|\|\| ..\|\|\|\|\| \|\|\| MutationRev 49 CATTTTGTTGAGTTGAAACAGA--------GAAAGTTGCTAAAC-TCA-- 87 Wildtype 484 TTTCTCAGTCAATTGCTGCCTCCTGAAATATTCGGGATCTAGGTACCCGA 533 \|.\|\|\| .\|\|.\|\|\| \|\|\|\|\|.\|.\| MutationRev 88 --TATCA------------------TAAGATT---------GGTACACAA 108 Wildtype 534 AGCTCCCTTTCACTGCCGTGCTAACATGAGTTTGATCGAGCTCGGGTCC- 582 \|\| \|.\|..\|\|.\|\|\|\| \|\| .\|\|\|.\|\|\| MutationRev 109 AG---------------GGGGAAAAATGA---------AG-ACGGATCCA 133 Wildtype 583 -TGCCTTAGAAAGTCCGAAATCAGCTACTTTTGCCATCATATTCTCATCA 631 \|\|\|\| \|\|..\|\|\|\| .\|\|\|\| MutationRev 134 ATGCC-TACCAAGT----------TTACT--------------------- 151 Wildtype 632 AGCAATATGTTTGCTGATTTGACATCACGATGTATAACTGCTTTGGCGTA 681 \|\|\|\|.\| \|\| \|\|.\|\|\|\|\|\| \|\| MutationRev 152 ---AATAGG-----------GA-------ATTTATAAC----------TA 170 Wildtype 682 GCTAGTATGAAGGTAGTGTAGACCTCT----GGCTGCCCCAATACAT--- 724 \| .\|\|\|.\|\|\| \|\|\| \|.\|.\|\|\|\| \|.\|\|\| \|\|\|.\|\| MutationRev 171 G-AAGTTTGA--GTA----AAAACTCTAGCAGTCTG-----ATAAATGGG 208 Wildtype 725 ---ATCTCCAGCCTCTG--TTTCCAGCTCATACTAGGCATATC---CGAA 766 \|\|\|.\|.\|\|.\|\|.\|\| \|\|..\|\|\|\|\|.\|\| \|\|\|\|\| .\|\|\| MutationRev 209 TGAATCACTAGACTTTGTATTATCAGCTAAT-------ATATCTTGAGAA 251 Wildtype 767 CCATACAGATGGCTCTTAAGGGTCCCATTTTCCATGTACTCGTACACGAG 816 .\|..\|\|\|\|\| ..\|\|\|\| \|.\|\|..\|\|\| MutationRev 252 ACTGACAGA---AACTTA-----------------------GAACCAGAG 275 Wildtype 817 AATCATCTCATTCTTTTCATCACAGTATCCAATCAATGAAACAAGATGAC 866 \| \|\|.\|\|\|\| \|\|.\|\|\|\|.\|...\| MutationRev 276 A--------------TTTATCA---------------GACACAAAAACCC 296 Wildtype 867 GATGACGGAACTGAGAGAGCATCTCTATTTCAGTTTGGAACTCTCTAAGA 916 .\|\|.\|\|..\|\|.\|.\|\| \|\|\|.\| \|\|\|\| \|\|\|\| MutationRev 297 TATTACCAAAATTAG----------TATCT---TTTG---------AAGA 324 Wildtype 917 CCTTGTTGGGAGCTAGGATTACCC---CTCTTAATAGCTACGTTCGTTCC 963 \|.\|.\| \|\|.\|\|\|\| \|\|.\| MutationRev 325 -------------------TCCACAGTCTATTAA-----------GTAC- 343 Wildtype 964 ATCATTTAAAACTCCCTTGAATACCTTACCAAAGCCACCTATCCCAATGA 1013 \|\|\| \|\|\|..\|\|.\|\| \|\|\|\|.\|\|\| \|\| MutationRev 344 -TCA--------TCCACTGGAT-------CAAATCCA-----------GA 366 Wildtype 1014 CCAAACTCTCATCAAACTTTTTGGTTGCTGCTAGCATGGCTGCAAAAGGA 1063 .\|\|\|\|\|.\|..\|..\|\|\|\| \|.\|.\|\|....\|.\|\|\|\|\| \| MutationRev 367 ACAAACACAGAAAAAAC-------------CCAACAACAGTACAAAA--A 401 Wildtype 1064 ATGCGATAACT 1074 \|\| .\|\|\|\| MutationRev 402 AT----AAACT 408

Feature Information

Sequence & Structure:

Name: Solyc06g005230 (Exon Intron) Length: 1125

The mutation and phenotype for selected feature: Solyc06g005230

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc06g005230 (Exon Intron)
Length: 1125