Name: Solyc06g071810
(Exon Intron)
Length: 1878
ATGACTTCGAATATCCACTTTTTTCTTTTATACGTTGTGAGCCTTTTCCTTTTTGTTCAAGCAAGATTGAATCTTTATCCACCAGATCATGCTGCACTCTTGCTTGTACAAAAGGACTTAGGCATCATTTCTGTTAACAACCCGTGTACGCTCGCAGGAATATCGTGCGAGCGTAGACCGGGTAACACAACACAAGTTGT
GAGAGTTACCCGTATTGTATTCAGATCCAACGGATTAAAGGGAACTTTGTCTTCTGCTATTGGCAAACTCACTGAGCTCAAAGAGCTTTCTCTTTCCGAC
AATCAACTATCTGAACAAATCCCCGTTCAGATTATTGATTGTCGGAAGTTAGAGATTCTTCAACTTCAAAGGAACAGATTTTCTGGGAAGATTCCATCCG
AATTGTCAGCTTTAAACCGTTTAAGGATAGTTGACTTTTCATCGAATGAGTTTTCGGGGAATCTTGATTTCTTGAAGTACTTTCCTAATCTGGAAAAACT
GTCTCTGGCTGATAATATGTTCACTGGAAAAATACCATTTTCATTGAAATCTTTCCGAAATCTTCGTTTTCTCAACATTTCAGGGAATAGTTTCCTTGAA
GGTCCAGTACCTGTCATGAGCCAAATTGAGCATTTATCAGCAGATTTGAATCGAAAAAATGGCGTTCCTAAACGTTACATTCTAGCTGAGAATTCAACAA
GGATATCTGCAATGGGACCTGCTTCGGTTCCAGCTCCAGCTCCAGCACCTGTCAATCGTGTTGTACCGGCGATGCATAAACGTAAGAACAAGAAAAGGAA
GTTACGATCTTGGTTTCTTGGTTTCCTAGCTGGAACTTTTGCTGGGGGTATATCTGCTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTA
AGAAGAGGGAATAACGATTCAAGTTTAACGATATACAGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGC
TTGAAATGATTGGAAAAGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCTACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATCCCC
AATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTCAAATTGTAGGTCAAATCAGACAC
CGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTCTACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGC
AAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGGACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTCCATATAAACCATACTCA
ACGCATAATTCACAGAGATTTAAAGCCAGCAAATATCCTACTTGATGATGACATGGAAGCTCGAGTTGCTGATTTTGGCCTTGCAAAGGCAGTTCCAGAT
GCTCATACACATATCACCACTTCAAATGTTGCAGGAACTGTTGGATTTATTGCACCAGAATATTATCAGACACTGAAGTTTACAGATAAGTGTGATATAT
ACAGCTTTGGTGTGGTGCTAGCTGTGTTGGTTATCGGTAAGGGTCCATCGGATGATTTTTTCCAACATACTTCTGAGATGAGTTTAGTTAAGTGGCTGAG
GAATGTAATGACTTCTGACGATCCTAAAATAGCAATTGATCCTAAGCTTATAGGAAATGGATACGACGAACAAATGCTTTTGGTTCTCAAGATAGCTTGC
TTTTGTACTCTCGACAATCCAAAGGAGAGGCCTAACAGTAAGGATGTTAGGTGCATGTTAACTCAGATCAAGCATTAG
The mutation and phenotype for selected feature: Solyc06g071810
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-1 (T0 ) | WT (50.6%); -1 bp (45.1%) | |
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-1-1 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-1-1-3 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-1-1-6 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-1-1-7 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: CTGTGCTCTGTTCCTTGCTATTCAAGATGGTAATGTTCTTCGTAAGAAGAGGGAATAACGATTCAAGTTTAACGATATAC AGTCCACTGATCAAGAAAGCCGAGGACTTGGCTTTCTTAGAGAAGGAAGATGGAGTGGCATCGCTTGAAATGATTGGAAA AGGTGGCTGTGGAGAAGTTTATAGAGCAGAGCACCGGGGAGTAATGGGAAGATTATAGCTATAAAGAAGATTATCCAATC CCCAATGGATGCTGCAGAGATCACGGAGGAAGATACTAAGGCGTTGAACAAGAAAATGCGTCAAGTTAAATCAGAAATTC AAATTGTAGGTCAAATCAGACACCGGAATCTGCTTCCATTACTCGCGCATATGCCAAGGCCAGACTGTCATTACTTGGTC TACGAATACATGAAAAATGGGAGTTTACAGGATATCCTGCAGCAAGTCACAGAAGGCACAAGGGAATTAGATTGGTTGGG ACGACACAGAATTGCAGCGGGGGTAGCTGCTGGTCTCGAGTATCTC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-4 (T0 ) | -94 bp | |
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-4-3 (T1 ) | -94 bp | |
| CRT0020 | RG-PtoR | CTG0020-1 | CGR0020-1 | CRT0020-5 (T0 ) | WT 70%; -1 bp 25% |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
