| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-1 (T0 ) |
-1 bp (63.1%); WT (29.3%); -2 bp (2.9%)
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-2 (T0 ) |
-1 bp (76%); WT (22%)
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-2-1 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTAT
TGGAAGCCATGAGGAACTCCGCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGATCTTCGTAT
TCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCC
ATAGCACACGCCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTAC
CTTCGGTGCTTTTGTTGGTGGAAACATCACTTTGTTCCGTGG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 63 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 112
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 50
Wildtype 113 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 162
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 100
Wildtype 163 GCCATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTG 212
|||||||||||||||||||||||||| |||||||||||||||||||||||
Mutation 101 GCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTG 149
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 213 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 262
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 150 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 199
Wildtype 263 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 312
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 249
Wildtype 313 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 362
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 299
Wildtype 363 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 412
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 349
Wildtype 413 TTTGTTCCGTGG 424
||||||||||||
Mutation 350 TTTGTTCCGTGG 361
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-2-3 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTAT
TGGAAGCCATGAGGAACTCCGCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGATCTTCGTAT
TCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCC
ATAGCACACGCCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTAC
CTTCGGTGCTTTTGTTGGTGGAAACATCACTTTGTTCCGTGG
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 63 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 112
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 CCAACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTC 50
Wildtype 113 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 162
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 GTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC 100
Wildtype 163 GCCATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTG 212
|||||||||||||||||||||||||| |||||||||||||||||||||||
Mutation 101 GCCATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTG 149
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 213 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 262
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 150 ATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTAC 199
Wildtype 263 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 312
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACG 249
Wildtype 313 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 362
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 CCTTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGC 299
Wildtype 363 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 412
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 CACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCAC 349
Wildtype 413 TTTGTTCCGTGG 424
||||||||||||
Mutation 350 TTTGTTCCGTGG 361
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-3 (T0 ) |
-1 bp (51.2%); -8 bp (43.1%)
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-3-4 (T1 ) |
-8 bp/-8 bp
Is tDNA present: No
Mutation sequence: TAGTGAAAATTTTCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCC
GCCATCCCGGGGCGCTTAA--------TGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGT
ATGGCTTTCAATAAGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTT
GTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTG
GAAACATCACTTTGTTCCGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 83 TAGTGAAAATTTTCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACC 132
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 TAGTGAAAATTTTCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACC 50
Wildtype 133 AAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGGCGCTTAAA 182
|||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGGCGCTTAA- 99
gRNA GGCGCTTAAA
Wildtype 183 GCGGCCTTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCA 232
|||||||||||||||||||||||||||||||||||||||||||
Mutation 100 -------TGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCA 142
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 233 GGGTTCTGGTATGGCTTTCAATAAGCTTACCGATGGCGTCGCTACTCCCG 282
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 143 GGGTTCTGGTATGGCTTTCAATAAGCTTACCGATGGCGTCGCTACTCCCG 192
Wildtype 283 CTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCC 332
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 193 CTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCC 242
Wildtype 333 GTCTCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTAC 382
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 243 GTCTCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTAC 292
Wildtype 383 CTTCGGTGCTTTTGTTGGTGGAAACATCACTTTGTTCCGT 422
||||||||||||||||||||||||||||||||||||||||
Mutation 293 CTTCGGTGCTTTTGTTGGTGGAAACATCACTTTGTTCCGT 332
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-4 (T0 ) |
-1 bp (88.9%); WT (9.2%)
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-4-3 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-4-9 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-5 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-5-11 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-6 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-7 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-7-2 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-7-2-3 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-7-2-7 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-8 (T0 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-8-5 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
|
| CRT0059 |
RG-prf3 |
CTG0059-1 |
CGR0059-2 |
CRT0059-8-5-2 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCCAACTTGTGCCCCCCTTAATAGTGAAAATTT
TCAATTGTTAATTTTCTCGTTAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGCCATCCCGGGG
CGCTTAAAGCGGCCTGGCGGAGTTCATCAGTACCCTGATCTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATA
AGCTTACCGATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCCTTCGGGTTGTTCGTCGCCGTC
TCCGTCGGTGCTAACATCTCCGGCGGCCACGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTTT
GTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCTGCTTGCGCTCTCCTTGAATTCGCCACCGGTG
GCATGGT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 15 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 64
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 1 ATTTCACAATATAAATCCATGCAAAACTTTCCCTAATTTCATCACTCCCC 50
Wildtype 65 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 114
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 51 AACTTGTGCCCCCCTTAATAGTGAAAATTTTCAATTGTTAATTTTCTCGT 100
Wildtype 115 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 164
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 101 TAAAAATGCCGATCAACCAAATTACTATTGGAAGCCATGAGGAACTCCGC 150
Wildtype 165 CATCCCGGGGCGCTTAAAGCGGCCTTGGCGGAGTTCATCAGTACCCTGAT 214
|||||||||||||||||||||||| |||||||||||||||||||||||||
Mutation 151 CATCCCGGGGCGCTTAAAGCGGCC-TGGCGGAGTTCATCAGTACCCTGAT 199
gRNA GGCGCTTAAAGCGGCCTTGG
Wildtype 215 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 264
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 200 CTTCGTATTCGCAGGTCAGGGTTCTGGTATGGCTTTCAATAAGCTTACCG 249
Wildtype 265 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 314
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 250 ATGGCGTCGCTACTCCCGCTGGCCTTATTTCCGCCTCCATAGCACACGCC 299
Wildtype 315 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 364
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 300 TTCGGGTTGTTCGTCGCCGTCTCCGTCGGTGCTAACATCTCCGGCGGCCA 349
Wildtype 365 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 414
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 350 CGTCAACCCTGCTGTTACCTTCGGTGCTTTTGTTGGTGGAAACATCACTT 399
Wildtype 415 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 464
||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation 400 TGTTCCGTGGAATTTTGTACATCATTGCACAGTTGCTTGGATCCACTGCT 449
Wildtype 465 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 502
||||||||||||||||||||||||||||||||||||||
Mutation 450 GCTTGCGCTCTCCTTGAATTCGCCACCGGTGGCATGGT 487
|
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| CRT0138 |
RG-PtoR |
CTG0138-23 |
CGR0138-23 |
CRT0138-57 (T0 ) |
-2 bp/-2 bp
|
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