Plant Crispr Database

Feature Information

Feature ID: Solyc06g084140
Feature Type: gene
Name: Transporter gene
Description: High affinity sulfate transporter 1
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc06g084140

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
CRT0138	RG-PtoR	CTG0138-26	CGR0138-26	CRT0138-28 (T0 )	didn't work with TIDE
CRT0138	RG-PtoR	CTG0138-26	CGR0138-26	CRT0138-28-2 (T1 )	-2 bp/-2 bp Is tDNA present: Yes Mutation sequence: AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCAATATTTATTTCCAATTTTTGAATGGGGTTC AAGATACAGTTTTGGTTTCTTCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCTCAAGGAATAA GCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACTATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAG GTAAAAAATTTATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCCACCATTAGTGTACGCATTAA TGGGAAGTTCAAGAGATTTAGCAGTGGGAACCGTTTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTGAA TCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCACTTTCTTCACCGGGTTATTCGAACTAGCACTTG GATTTTTCAGGTACGTAATGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTATTTTTAGGCTGGG ATTTATAGTGGATTTTCTATCACATGCAACCATTGTGGGATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAAT TAAAAGGAATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTTACGTTCTGTATTTGCACAAACG CACGCGGTACG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 224 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA 273 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA 50 Wildtype 274 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT 323 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT 100 Wildtype 324 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT 373 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT 150 Wildtype 374 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT 423 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT 200 Wildtype 424 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT 473 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT 250 Wildtype 474 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC 523 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 251 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC 300 Wildtype 524 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA 573 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 301 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA 350 gRNA GCAGTGGGAA Wildtype 574 CCGTGGCTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG 623 \|\|\|\| .\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 351 CCGT--TTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG 398 gRNA GCAGTGGGAACCGTGGCTGT Wildtype 624 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC 673 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 399 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC 448 Wildtype 674 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA 723 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 449 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA 498 Wildtype 724 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT 773 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 499 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT 548 Wildtype 774 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG 823 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 549 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG 598 Wildtype 824 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA 873 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 599 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA 648 Wildtype 874 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT 923 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 649 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT 698 Wildtype 924 ACGTTCTGTATTTGCACAAACGCACGCGGTACG 956 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 699 ACGTTCTGTATTTGCACAAACGCACGCGGTACG 731

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0138

RG-PtoR

CTG0138-26

CGR0138-26

CRT0138-28 (T0 )

didn't work with TIDE

CRT0138

RG-PtoR

CTG0138-26

CGR0138-26

CRT0138-28-2 (T1 )

-2 bp/-2 bp

Is tDNA present: Yes

Mutation sequence:

AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCAATATTTATTTCCAATTTTTGAATGGGGTTC
AAGATACAGTTTTGGTTTCTTCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCTCAAGGAATAA
GCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACTATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAG
GTAAAAAATTTATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCCACCATTAGTGTACGCATTAA
TGGGAAGTTCAAGAGATTTAGCAGTGGGAACCGTTTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTGAA
TCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCACTTTCTTCACCGGGTTATTCGAACTAGCACTTG
GATTTTTCAGGTACGTAATGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTATTTTTAGGCTGGG
ATTTATAGTGGATTTTCTATCACATGCAACCATTGTGGGATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAAT
TAAAAGGAATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTTACGTTCTGTATTTGCACAAACG
CACGCGGTACG

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype         224 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA    273
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 AAGCAATTCAAGAACCAAAAACCTTTGAGAAAATTTATACTTGGGGTTCA     50

Wildtype         274 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT    323
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 ATATTTATTTCCAATTTTTGAATGGGGTTCAAGATACAGTTTTGGTTTCT    100

Wildtype         324 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT    373
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCAAAGCAGATCTTATTGCTGGAATTACTATAGCTAGTCTTGCTATTCCT    150

Wildtype         374 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT    423
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 CAAGGAATAAGCTATGCTAAGCTGGGTAACTTGCCACCTATACTTGGACT    200

Wildtype         424 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT    473
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         201 ATGTAAGTCTTTTGTTATATTATTTTCTTTTTAGATAAAGGTAAAAAATT    250

Wildtype         474 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC    523
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         251 TATTTTGATGCATGCATAATTTTTGGTGGTTCAGATTCAAGCTTTATTCC    300

Wildtype         524 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA    573
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         301 ACCATTAGTGTACGCATTAATGGGAAGTTCAAGAGATTTAGCAGTGGGAA    350
gRNA                                                         GCAGTGGGAA

Wildtype         574 CCGTGGCTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG    623
                     ||||  .|||||||||||||||||||||||||||||||||||||||||||
Mutation         351 CCGT--TTGTTGGATCTCTTCTCATGGCTTCTATGTTGGGAGCTCAAGTG    398
gRNA                 GCAGTGGGAACCGTGGCTGT

Wildtype         624 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC    673
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         399 AATCCTGTTGAAAATCCAACACTTTATCTTCATCTTGCTTTCACTGCCAC    448

Wildtype         674 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA    723
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         449 TTTCTTCACCGGGTTATTCGAACTAGCACTTGGATTTTTCAGGTACGTAA    498

Wildtype         724 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT    773
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         499 TGGAACATGTTTTATAATATTAAAATAGTTGATGGTAATGAATGTTTTAT    548

Wildtype         774 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG    823
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         549 TTTTAGGCTGGGATTTATAGTGGATTTTCTATCACATGCAACCATTGTGG    598

Wildtype         824 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA    873
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         599 GATTTATGGGAGGAGCAGCTACAGTGGTCATACTCCAACAATTAAAAGGA    648

Wildtype         874 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT    923
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         649 ATACTTGGTCTTCAACATTTCACTCATGCCACGGATGTTATCTCTGTCTT    698

Wildtype         924 ACGTTCTGTATTTGCACAAACGCACGCGGTACG    956
                     |||||||||||||||||||||||||||||||||
Mutation         699 ACGTTCTGTATTTGCACAAACGCACGCGGTACG    731

Feature Information

Sequence & Structure:

Name: Solyc06g084140 (Exon Intron) Length: 4087

The mutation and phenotype for selected feature: Solyc06g084140

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc06g084140 (Exon Intron)
Length: 4087