Plant Crispr Database

Feature Information

Feature ID: Solyc08g006320
Feature Type: gene
Name: WRKY11
Description: WRKY transcription factor 11
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc08g006320

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation
CRT0119	RG-PtoR	CTG0119-2	CGR0119-2	CRT0119-1 (T0 )	WT (57.9%); -5 bp (35.5%); +1 bp (2.3%)
CRT0119	RG-PtoR	CTG0119-2	CGR0119-2	CRT0119-1-2 (T1 )	-4 bp/-4 bp Is tDNA present: No Mutation sequence: CTGCAAATCAAACAACAAAAATGGCTGTAGATTTGTTAAATTATTCGAATTTGAATGAACAATTAGCTTTACAAGAAGCT GCTACAGCCGGTTTAAAATCTATGGATAATTTAATCCGGTTCGTTTCATTTCAACAACAGCAAAATCAAACGGTTCAGCC GGATTGTAGAGAGATAACTGATTATACTGTTTCGAATTTTAGAAAGGTTATTACTATTTTGAACCGGACCGGTCATGCCC GGTTTAGACGTAGTCCGGTTCAGGTTACTGATGATCTACGGCTTTAACTCTATCGCCGTTGACAAATCCGGCGGAGGAAA CTGTACCGGCGGTGAAAGTACCGGTTGAGAAGTATCAATCGAAGGCGTTGACTTTAGATTTTACGAAACGGAAAGTTGGT AAATCTATTGGATGTGAAGCTGTTCCGGTAGCGAGTTCGACGACGTCGTCGTCGTTTATGTCGACGATTACAGGTGAAGG AAGTGTTTCGAATGGTAAAGTGTTTTCGTCGATGAGTTTACCTCCTCGTCCGCCTGTTTCCTCCGGGAAGCCGCCAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 896 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 945 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 50 Wildtype 946 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 995 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 100 Wildtype 996 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 1045 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 150 Wildtype 1046 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 1095 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 200 Wildtype 1096 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 1145 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 250 Wildtype 1146 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAATCATCAGTAACC 1195 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAG----ATCATCAGTAACC 296 gRNA TAGACGAATCATCAGTAACC Wildtype 1196 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 1245 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 297 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 346 Wildtype 1246 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 1295 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 347 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 396 Wildtype 1296 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 1345 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 397 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 446 Wildtype 1346 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 1395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 447 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 496 Wildtype 1396 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 1445 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 497 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 546 Wildtype 1446 TTTGATTTGCAG 1457 \|\|\|\|\|\|\|\|\|\|\|\| MutationRev 547 TTTGATTTGCAG 558
CRT0119	RG-PtoR	CTG0119-2	CGR0119-2	CRT0119-1-4 (T1 )	azygous Is tDNA present: Yes Mutation sequence: TGGCTGTAGATTTGTTAAATTATTCGAATTTGAATGAACAATTAGCTTTACAAGAAGCTGCTACAGCCGGTTTAAAATCT ATGGATAATTTAATCCGGTTCGTTTCATTTCAACAACAGCAAAATCAAACGGTTCAGCCGGATTGTAGAGAGATAACTGA TTATACTGTTTCGAATTTTAGAAAGGTTATTACTATTTTGAACCGGACCGGTCATGCCCGGTTTAGACGTAGTCCGGTTC AGGTTACTGATGATTCGTCTACGGCTTTAACTCTATCGCCGTTGACAAATCCGGCGGAGGAAACTGTACCGGCGGTGAAA GTACCGGTTGAGAAGTATCAATCGAAGGCGTTGACTTTAGATTTTACGAAACGGAAAGTTGGTAAATCTATTGGATGTGA AGCTGTTCCGGTAGCGAGTTCGACGACGTCGTCGTCGTTTATGTCGACGATTACAGGTGAAGGAAGTGTTTCGAATGGTA AAGTGTTTTCGTCGATGAGTTTACCTCCTCGTCCGCCTGTTTCCTCCGGGAAGCCGCCAATCGCCGGGAAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 885 TTTTCCCGGCGATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGT 934 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 TTTTCCCGGCGATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGT 50 Wildtype 935 AAACTCATCGACGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGT 984 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 AAACTCATCGACGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGT 100 Wildtype 985 AATCGTCGACATAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAG 1034 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AATCGTCGACATAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAG 150 Wildtype 1035 CTTCACATCCAATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTC 1084 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 CTTCACATCCAATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTC 200 Wildtype 1085 AACGCCTTCGATTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGT 1134 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AACGCCTTCGATTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGT 250 Wildtype 1135 TTCCTCCGCCGGATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAAT 1184 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TTCCTCCGCCGGATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAAT 300 gRNA TAGACGAAT Wildtype 1185 CATCAGTAACCTGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGG 1234 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 CATCAGTAACCTGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGG 350 gRNA TAGACGAATCATCAGTAACC Wildtype 1235 TTCAAAATAGTAATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTAT 1284 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TTCAAAATAGTAATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTAT 400 Wildtype 1285 CTCTCTACAATCCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAA 1334 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CTCTCTACAATCCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAA 450 Wildtype 1335 CGAACCGGATTAAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCT 1384 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 CGAACCGGATTAAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCT 500 Wildtype 1385 TGTAAAGCTAATTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGC 1434 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 TGTAAAGCTAATTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGC 550 Wildtype 1435 CA 1436 \|\| MutationRev 551 CA 552
CRT0119	RG-PtoR	CTG0119-2	CGR0119-2	CRT0119-1-5 (T1 )	-4 bp/-4 bp Is tDNA present: Yes Mutation sequence: CTGCAAATCAAACAACAAAAATGGCTGTAGATTTGTTAAATTATTCGAATTTGAATGAACAATTAGCTTTACAAGAAGCT GCTACAGCCGGTTTAAAATCTATGGATAATTTAATCCGGTTCGTTTCATTTCAACAACAGCAAAATCAAACGGTTCAGCC GGATTGTAGAGAGATAACTGATTATACTGTTTCGAATTTTAGAAAGGTTATTACTATTTTGAACCGGACCGGTCATGCCC GGTTTAGACGTAGTCCGGTTCAGGTTACTGATGATCTACGGCTTTAACTCTATCGCCGTTGACAAATCCGGCGGAGGAAA CTGTACCGGCGGTGAAAGTACCGGTTGAGAAGTATCAATCGAAGGCGTTGACTTTAGATTTTACGAAACGGAAAGTTGGT AAATCTATTGGATGTGAAGCTGTTCCGGTAGCGAGTTCGACGACGTCGTCGTCGTTTATGTCGACGATTACAGGTGAAGG AAGTGTTTCGAATGGTAAAGTGTTTTCGTCGATGAGTTTACCTCCTCGTCCGCCTGTTTCCTCCGGGAAGCCGCCAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 896 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 945 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ATTGGCGGCTTCCCGGAGGAAACAGGCGGACGAGGAGGTAAACTCATCGA 50 Wildtype 946 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 995 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CGAAAACACTTTACCATTCGAAACACTTCCTTCACCTGTAATCGTCGACA 100 Wildtype 996 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 1045 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 TAAACGACGACGACGTCGTCGAACTCGCTACCGGAACAGCTTCACATCCA 150 Wildtype 1046 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 1095 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 ATAGATTTACCAACTTTCCGTTTCGTAAAATCTAAAGTCAACGCCTTCGA 200 Wildtype 1096 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 1145 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TTGATACTTCTCAACCGGTACTTTCACCGCCGGTACAGTTTCCTCCGCCG 250 Wildtype 1146 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAGACGAATCATCAGTAACC 1195 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 GATTTGTCAACGGCGATAGAGTTAAAGCCGTAG----ATCATCAGTAACC 296 gRNA TAGACGAATCATCAGTAACC Wildtype 1196 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 1245 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 297 TGAACCGGACTACGTCTAAACCGGGCATGACCGGTCCGGTTCAAAATAGT 346 Wildtype 1246 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 1295 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 347 AATAACCTTTCTAAAATTCGAAACAGTATAATCAGTTATCTCTCTACAAT 396 Wildtype 1296 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 1345 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 397 CCGGCTGAACCGTTTGATTTTGCTGTTGTTGAAATGAAACGAACCGGATT 446 Wildtype 1346 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 1395 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 447 AAATTATCCATAGATTTTAAACCGGCTGTAGCAGCTTCTTGTAAAGCTAA 496 Wildtype 1396 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 1445 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 497 TTGTTCATTCAAATTCGAATAATTTAACAAATCTACAGCCATTTTTGTTG 546 Wildtype 1446 TTTGATTTGCAG 1457 \|\|\|\|\|\|\|\|\|\|\|\| MutationRev 547 TTTGATTTGCAG 558

Feature Information

Sequence & Structure:

Name: Solyc08g006320 (Exon Intron) Length: 1468

The mutation and phenotype for selected feature: Solyc08g006320

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc08g006320 (Exon Intron)
Length: 1468