Name: Solyc08g066840
(Exon Intron)
Length: 2268
TCCAGACAAAACTTCAATTTCATCTTCTGATTTCATATCATGATACCAATATCACATGACCAAACGGGGTAGTTTAGTACTTTAGTTTCGTAATGAAATAATCGTAGCAAGTCTAGCGAAAGTATTATTAAAATATAAAGTTACAAAAAGGATAACATAAAAAATACATGCATAGATAAGGTAGACTTCCAAAACTACAA
GAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAAAAAGTTTTCACAAATAAAACC
AGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATTCCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATT
GAAGCTCCTGAAAAAGGCCCACCAGCCATAATATTGGCCCCAACAACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCA
CAAGTGTTGTATACACAGTGAACAACAAAGAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGG
TGTTATCTAAAAAAAAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATCGTTGATTAAAACGGT
GTGAAATGAGTACTTACCATTCCATCGGTGAGGTAATTGAGCAAAGCACAAGCAGCAACAGAAGCAAGTAATTGGTCAATCCAGTAAAGGATTGATCTAA
AGACAGTGATATGACCACCCATACAAAGGCCAAGTGTGACAGCAGGGTTAAGGTGCCCACCAGAAATACGAAAACCAGCAGAGATTGTCACAGCCACCAC
CAATGCATGTGCCATTGCCACAAAAAATAAACTCACTAGTGGATCTCCATTTAGCTTATCTGTTTTCAATCAAAAATATGTTCGAACACTCTTTAATTTT
GTGATTTTAAATATGTAAGTGGAAAGTTGAAAGGTTTCGAGAAATTCATCCGTGTTTTAAACAGACTTCAAAAGAAAATAAGTCAAACAAATTAAAATGC
AAAAAATTAGTGAATTGTCATGTATAATAATAATCAAGAGAGAATTAATTATCTTTTTTCAACTTTGCCCTTAACATTAATTATAAGAATTAATATAAGT
GTATATACTATTGATCAAACAACTCTCTTTATCAAGACAACTAAAAAATAAATAAAAAGACAGTCTTATACATTAAGTGTCCGCTATATATGTGCGGGAT
TCAGAAAATAACCAGACCATAAAAAGCGAAAGAGGATCTCCATTTAGCTTATCTGTTTTCATTCAAAAATACTCTAATTTGATTATGCATCCCTTTTAAT
TATATAGCCAAATGTACACAAGTTATACCCTCTCAATTTATTTGATTTTGATACAATTTAAGAGAGTAAAAAACTTTTTAATATATCAAATACACTTTAA
TTTTGTGGTTTTGTTGAAAGGTTTGGAGAAATTCATTCTTATTTTAAACAGACTTACAAAGAAAATAGGTCAAACAAATTGTAATGAAAAATTAGTCAAT
TGTCGTGTATTAATCAATAGAGAATTAATTATCTTTTTTCAACTTTGCCCTTAACATTAATTATTCTAAAATTAAAAGACACATATATAGATAAAAATTA
AAGAATTAATTTAAGTGTATATACTATTGATCAAACAACACTCTATATCAAGACAACTCAAAAACGAACAAAAAGACGACAATCTTATGCATTAAGTGCA
CGCTATATATGCGCGGGATTCAGAGAAGAACCGAACCATAAAAAGCAAAAGAGGCATAATTGATGGGTGCAAACGTAACTTACTTGCAGCCATGGCAGAT
CCAACACCAGCAAAAACAAAGAGAAAAGTGCAAATAAATTCAACAATGAGGGCTTGGATGCAATCAGGCTGAATAGCCTCACGGCTACTTCCAACAGCAA
TTTTCGCCATTTTAATTTCTCGTCAAATTAATGAAATGGTTTTATTTTTGCTTTTAAAACGGAGGAGGACCATGTACAGCTATATATTTATACACACTCG
TAGAATAATATTTAATTTAGCTAGGGTGGCAGACAGTGATGAAACTGATTTTACGAATAAATAATTACATATTTGAATAAAAAAATTTAGATCGATAATA
AATAGTTGATATGTTTAGAGAGAAAAAAAAGTCACGTCAAGTGTTGCTCGTAAGACAAGAATATCGGT
The mutation and phenotype for selected feature: Solyc08g066840
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-3 (T0 ) | -12 bp (50.5%); -6 bp (44.9%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-3-4 (T1 ) |
-6 bp/-6 bp
Is tDNA present: No
Mutation sequence: ATTCTTTGTTAATATGTACATTTTTTTTTTAGATAACACCAGTTCACACCTTAGCAAATGGAATGAGCTATGGGCAGGGG TTAATTATGGAGGTGATTTTGACCTTTTCTTTGTTGTTCACTGTGTATACAACACTTGTGGACCCCAAGAAAGGAATTCT TGAAGGAATGGGCCCACTTCTAACTGGGCTTGTTGTTGGGGCCAATATT------GGTGGGCCTTTTTCAGGAGCTTCAA TGAACCCAGCAAGGTCATTTGGGCCAGCTTTTGTAAGTGGAATCTGGACTGACCATTGGGTCTACTGGATTGGGCCTTTC ATTGGTGGTGGGCTTGCTGGTTTTATTTGTGAAAACTTTTTCATTGTTAGGTCTCATGTTCCTCTTCCAAATGAAGAGAC TTTCTAGGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-4 (T0 ) | -15 bp (64.2%); WT (30.6%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-4-3 (T1 ) |
-15 bp/-15 bp
Is tDNA present: No
Mutation sequence: AGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATTC CACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCCCCAACAACAAGCCCA GTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAAGAAAA GGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAA AAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-4-9 (T1 ) |
-15 bp/-15 bp
Is tDNA present: No
Mutation sequence: AGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGAA AAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATTC CACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCCCCAACAACAAGCCCA GTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAACAAAGAAAA GGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAA AAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-6 (T0 ) | WT 42%; +1 (56.2%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-7 (T0 ) | -7 bp (46.8%); -9 bp (50.5%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-7-2 (T1 ) |
-7 bp/-7 bp
Is tDNA present: Yes
Mutation sequence: AAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGA AAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATT CCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCATATTGGCCCCAACA ACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAA CAAAGAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTA TCTAAAAAAAAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATC GTTGATTAAAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-7-2-3 (T2 ) |
-7 bp/-7 bp
Is tDNA present: Yes
Mutation sequence: AAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGA AAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATT CCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCATATTGGCCCCAACA ACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAA CAAAGAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTA TCTAAAAAAAAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATC GTTGATTAAAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-7-2-7 (T2 ) |
-7 bp/-7 bp
Is tDNA present: Yes
Mutation sequence: AAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAATGA AAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAGATT CCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCATATTGGCCCCAACA ACAAGCCCAGTTAGAAGTGGGCCCATTCCTTCAAGAATTCCTTTCTTGGGGTCCACAAGTGTTGTATACACAGTGAACAA CAAAGAAAAGGTCAAAATCACCTCCATAATTAACCCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTA TCTAAAAAAAAAATGTACATATTAACAAAGAATTTCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATC GTTGATTAAAAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-8 (T0 ) | Large deletion? TIDE didn't work well | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-8-5 (T1 ) |
-124 bp/-124 bp
Is tDNA present: Yes
Mutation sequence: TACAAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAA TGAAAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAG ATTCCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCTCCATAATTAAC CCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTAACAAAGAATT TCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATCGTTGATTAAAACGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-3 | CRT0059-8-5-2 (T2 ) |
-124 bp/-124 bp
Is tDNA present: Yes
Mutation sequence: TACAAGAATAGATAAAGAGCCTTTTGTTACAAAACCCTAGAAAGTCTCTTCATTTGGAAGAGGAACATGAGACCTAACAA TGAAAAAGTTTTCACAAATAAAACCAGCAAGCCCACCACCAATGAAAGGCCCAATCCAGTAGACCCAATGGTCAGTCCAG ATTCCACTTACAAAAGCTGGCCCAAATGACCTTGCTGGGTTCATTGAAGCTCCTGAAAAAGGCCCACCTCCATAATTAAC CCCTGCCCATAGCTCATTCCATTTGCTAAGGTGTGAACTGGTGTTATCTAAAAAAAAAATGTACATATTAACAAAGAATT TCAAAACTTTTATCATTAACTGTGTCATTAGATCTTAAATTTCATCGTTGATTAAAACGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
