Plant Crispr Database

Feature Information

Feature ID: Solyc08g081210
Feature Type: gene
Name: MKKK66
Description: MAPKKK 66
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc08g081210

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation
CRT0085	RG-PtoR	CTG0085-1	CGR0085-1	CRT0085-1 (T0 )	+1 bp (50.9%); WT (46.8%)
CRT0085	RG-PtoR	CTG0085-1	CGR0085-1	CRT0085-1-10 (T1 )	+1 bp/+1 bp Is tDNA present: Yes Mutation sequence: AAGGATGTAAGGAGGAAATCCACTAAGGAGAGTTTCATTGACATAATAAATCGGAAACTGAAGATTTTCACCACGGAAAA ATCAAGTGGTAAATCTGGATCATCTCGAAGACGACGTAAAGATAAAAATTCAGTGAAGGGTTCTCAATCAAGGGTTTCAA GGTCACCATCACCATCTACGCCAGATTCACGCAAGTCAGGTCTTTGCTGATAGGACTTCTTCTCAACCACTTCCCCTTCC TGAGGGTCACTCTTCTAATGTACATCTTGTCGACTCTGATAACAGTGGATCCATAATATTAGTGACCGGTGAAGTCTCCG AGCCATCATTGACTTTGCCTCTTCCCATGCCCAGGCATCTTCCACATGGACCAACTGCTGCAGGAGTTGACAGGGACTTA CCAACTGCTTCTGTTTCTTGTGACTGCTCCAGTGACAGTGATGATCTTACTGACTCACGATTTCTAAGTCCCCAAACATC TGATTATGAAAACGGGAGCAGAACTGCCTTGAATAGTCCTTCCAGGTAAGCACAAACTGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 5881 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 5930 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 50 Wildtype 5931 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 5980 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 100 Wildtype 5981 CACTGTCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 6030 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CACTGTCACTGGAGCAGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 150 Wildtype 6031 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 6080 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 200 Wildtype 6081 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 6130 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 250 Wildtype 6131 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 6180 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 300 Wildtype 6181 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGAC-TGC 6229 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| MutationRev 301 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTTGC 350 gRNA GAC-TGC Wildtype 6230 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 6279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 400 gRNA GACTGCGTGAATCTGGCGT Wildtype 6280 CCCTTCACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 6329 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CCCTTCACTGAATTTTTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 450 Wildtype 6330 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 6379 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 500 Wildtype 6380 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 6419 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 540
CRT0085	RG-PtoR	CTG0085-1	CGR0085-1	CRT0085-1-4 (T1 )	azygous Is tDNA present: No Mutation sequence: TGCTCACGCTAATTTTATATAGCAATGCGTTCATGGTGGGGGAAGTCTTCATCTAAGGATGTAAGGAGGAAATCCACTAA GGAGAGTTTCATTGACATAATAAATCGGAAACTGAAGATTTTCACCACGGAAAAATCAAGTGGTAAATCTGGATCATCTC GAAGACGACGTAAAGATACAAATTCAGTGAAGGGTTCTCAATCAAGGGTTTCAAGGTCACCATCACCATCTACGCCAGAT TCACGCAGTCAGGTCTTTGCTGATAGGACTTCTTCTCAACCACTTCCCCTTCCTGAGGGTCACTCTTCTAATGTACATCT TGTCGACTCTGATAACAGTGGATCCATAATATTAGTGACCGGTGAAGTCTCCGAGCCATCATTGACTTTGCCTCTTCCCA TGCCCAGGCATCTTCCACATGGACCAACTGCTGCAGGAGTTGACAGGGACTTACCAACTGCTTCTGTTTCTTGTGACAGC TCCAGTGACAGTGATGATCTTACTGACTCACGATTTCTAAGTCCCCAAACATCTGATTATGAAAACGGGAGCAGAACTGC CTTGAATAGTCCTTCCAGGTAAGCACAAACTGTAACAATTGCTGTTCCATCTCGAATTTGATATACAGCTGTGCTTAAAA GTATCTCTTTTGACTTCTTTATCAAAAAAAAAAAAAATTCTTCATCAAAAAGTATCTCTTTTGAGCATTTCTAACAAATA AATTCAATTTCTGCAGTTTGAAGCAGAAGGTTCAGTCCCCTATTGCATCCAATGCAAGCTCAGGAGAGATGCTGAAGTCA GCTACTCTTTTGTCAGACAATCAGGCGATCCCTACATC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 5636 GATGTAGGGATCGCCTGATTGTCTGACAAAAGAGTAGCTGACTTCAGCAT 5685 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 GATGTAGGGATCGCCTGATTGTCTGACAAAAGAGTAGCTGACTTCAGCAT 50 Wildtype 5686 CTCTCCTGAGCTTGCATTGGATGCAATAGGGGACTGAACCTTCTGCTTCA 5735 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 CTCTCCTGAGCTTGCATTGGATGCAATAGGGGACTGAACCTTCTGCTTCA 100 Wildtype 5736 AACTGCAGAAATTGAATTTATTTGTTAGAAATGCTCAAAAGAGATACTTT 5785 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 AACTGCAGAAATTGAATTTATTTGTTAGAAATGCTCAAAAGAGATACTTT 150 Wildtype 5786 TTGATGAAGAATTTTTTTTTTTTTTGATAAAGAAGTCAAAAGAGATACTT 5835 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TTGATGAAGAATTTTTTTTTTTTTTGATAAAGAAGTCAAAAGAGATACTT 200 Wildtype 5836 TTAAGCACAGCTGTATATCAAATTCGAGATGGAACAGCAATTGTTACAGT 5885 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 TTAAGCACAGCTGTATATCAAATTCGAGATGGAACAGCAATTGTTACAGT 250 Wildtype 5886 TTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTTTTCAT 5935 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 TTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTTTTCAT 300 Wildtype 5936 AATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCATCACTG 5985 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 301 AATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCATCACTG 350 Wildtype 5986 TCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTGTCAAC 6035 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 TCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTGTCAAC 400 Wildtype 6036 TCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAGAGGCA 6085 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 TCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAGAGGCA 450 Wildtype 6086 AAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGGATCCA 6135 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 AAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGGATCCA 500 Wildtype 6136 CTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCAGGAAG 6185 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCAGGAAG 550 Wildtype 6186 GGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTGCGTGAAT 6235 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 551 GGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTGCGTGAAT 600 gRNA GACTGCGTGAAT Wildtype 6236 CTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAACCCTTC 6285 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 601 CTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAACCCTTC 650 gRNA GACTGCGTGAATCTGGCGT Wildtype 6286 ACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTTACCACT 6335 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 651 ACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTTACCACT 700 Wildtype 6336 TGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGTCAATGA 6385 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 701 TGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGTCAATGA 750 Wildtype 6386 AACTCTCCTTAGTGGATTTCCTCCTTACATCCTTAGATGAAGACTTCCCC 6435 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 751 AACTCTCCTTAGTGGATTTCCTCCTTACATCCTTAGATGAAGACTTCCCC 800 Wildtype 6436 CACCATGAACGCATTGCTATATAAAATTAGCGTGAGCA 6473 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 801 CACCATGAACGCATTGCTATATAAAATTAGCGTGAGCA 838
CRT0085	RG-PtoR	CTG0085-1	CGR0085-1	CRT0085-1-6 (T1 )	+1 bp/+1 bp Is tDNA present: Yes Mutation sequence: AAGGATGTAAGGAGGAAATCCACTAAGGAGAGTTTCATTGACATAATAAATCGGAAACTGAAGATTTTCACCACGGAAAA ATCAAGTGGTAAATCTGGATCATCTCGAAGACGACGTAAAGATAAAAATTCAGTGAAGGGTTCTCAATCAAGGGTTTCAA GGTCACCATCACCATCTACGCCAGATTCACGCAAGTCAGGTCTTTGCTGATAGGACTTCTTCTCAACCACTTCCCCTTCC TGAGGGTCACTCTTCTAATGTACATCTTGTCGACTCTGATAACAGTGGATCCATAATATTAGTGACCGGTGAAGTCTCCG AGCCATCATTGACTTTGCCTCTTCCCATGCCCAGGCATCTTCCACATGGACCAACTGCTGCAGGAGTTGACAGGGACTTA CCAACTGCTTCTGTTTCTTGTGACTGCTCCAGTGACAGTGATGATCTTACTGACTCACGATTTCTAAGTCCCCAAACATC TGATTATGAAAACGGGAGCAGAACTGCCTTGAATAGTCCTTCCAGGTAAGCACAAACTGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 5881 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 5930 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 1 ACAGTTTGTGCTTACCTGGAAGGACTATTCAAGGCAGTTCTGCTCCCGTT 50 Wildtype 5931 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 5980 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 51 TTCATAATCAGATGTTTGGGGACTTAGAAATCGTGAGTCAGTAAGATCAT 100 Wildtype 5981 CACTGTCACTGGAGCTGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 6030 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 101 CACTGTCACTGGAGCAGTCACAAGAAACAGAAGCAGTTGGTAAGTCCCTG 150 Wildtype 6031 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 6080 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 151 TCAACTCCTGCAGCAGTTGGTCCATGTGGAAGATGCCTGGGCATGGGAAG 200 Wildtype 6081 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 6130 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 201 AGGCAAAGTCAATGATGGCTCGGAGACTTCACCGGTCACTAATATTATGG 250 Wildtype 6131 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 6180 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 251 ATCCACTGTTATCAGAGTCGACAAGATGTACATTAGAAGAGTGACCCTCA 300 Wildtype 6181 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGAC-TGC 6229 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\| MutationRev 301 GGAAGGGGAAGTGGTTGAGAAGAAGTCCTATCAGCAAAGACCTGACTTGC 350 gRNA GAC-TGC Wildtype 6230 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 6279 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 351 GTGAATCTGGCGTAGATGGTGATGGTGACCTTGAAACCCTTGATTGAGAA 400 gRNA GACTGCGTGAATCTGGCGT Wildtype 6280 CCCTTCACTGAATTTGTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 6329 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 401 CCCTTCACTGAATTTTTATCTTTACGTCGTCTTCGAGATGATCCAGATTT 450 Wildtype 6330 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 6379 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 451 ACCACTTGATTTTTCCGTGGTGAAAATCTTCAGTTTCCGATTTATTATGT 500 Wildtype 6380 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 6419 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| MutationRev 501 CAATGAAACTCTCCTTAGTGGATTTCCTCCTTACATCCTT 540
CRT0085	RG-PtoR	CTG0085-1	CGR0085-1	CRT0085-1-7 (T1 )	+1 bp (92%); +2 bp (3%)

Feature Information

Sequence & Structure:

Name: Solyc08g081210 (Exon Intron) Length: 6591

The mutation and phenotype for selected feature: Solyc08g081210

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Name: Solyc08g081210 (Exon Intron)
Length: 6591