Name: Solyc08g082990
(Exon Intron)
Length: 3439
ATGGAAGAATCTGCATCAGAAATCAAGACTCCTCTGTGTAAGACTCTTTTTTCTCCAACACAATTTAATTCTTTAAAAATGTCGATATTAGATCCTAAAAAAACGAATCAGTTCTCACACGTTGTAACAACATTTATGAAAGGATCCGAAAGGTGTATTTCAGATCCAAAACTGAAATCTCTCTCTGTTTTGTAGTATCC
AATTTTGATCATGGCATTGCATCAAGCTCTAATGTTAAAGTCCACAAGGATGATGAAGATGAAAATTCTCCTGTCGAACAAGTTGCTCTTACTGTTCCAA
CAACTGATGATCCTTCATTACCTGTTTTAACTTTTCGAATGTGGTTTTTAGGGACAGTTTCTTGTGTTTTGTTATCATTTCTGAACCAATTCTTTTGGTA
CAGAACAGAGCCATTGACAATTACAGCAATTTCAGCTCAAATAGCTGTGGTTCCTTTGGGTCAACTTATGGCTGCAAAGATAACAAAAAGGGTATTTTTG
CAAGGATCTAAATGGGAATTCAGTTTAAATCCAGGCCCTTTTAATGTTAAAGAACATGTCCTCATCACCATTTTTGCAAATTCTGGTGCTGGAACTGTTT
ATGCTATTCATGTTGTCACTGCTGTTAAAGTCTTTTATAAGAAAGACATTACCTTCTTTGTTTCTCTTATTGTTATCATCACAACACAGGTAATTAATCG
TAACGACTAACTGACGACGATGACGTATATAACTTAAACTTTGTTAATAAGATATTTTAAATGGTAGGTGTTGGGATTTGGATGGGCTGGAATTTTCAGA
AAGTACTTGGTTGAGCCAGCAGCCATGTGGTGGCCTTCCAATCTTGTCCAAGTCTCATTATTCAGGTAAGTTTTGTAAAATAATTTTCAAACCTCTTTGT
CGAAAAGTTCAAGGAGAAAAAAATAATTTTTATACAAGATAAATTTCTGCTAAATAGTTTCAAGAGTATTTAGACGGATCACTATTATTATTTTTGGTAG
GTTGCGGCTGATAAATACGACAGCGATCCCACAGGTTGCTTTTGTTTTTGAAAATAATATCTTTGGGAATTAGGTGAAAAATCAATAATTTTAGAAGATA
TTTTATATGGAAATTTCATTATGTTAGGTAATTAGATATATATCAATTTTGTTTTTCTTGAAAACAGAGTAATAGTAATGGAGGATCCTTTCACCTAAAA
AAGATTCATGTTTTTATTCTATAGTAATGTAATAATTCCTCTCAAGATTAAACTTTTATCCACTTTTCGTATAATTGCCACAAATTAGTGAACTGAATTG
TAGATTCAAACTCGTAACACTGTGATTCAAGTTATAACCATAGGATGTCCCATAGGGGACTACTCTGCTTCACAGGAATTGATTGCCAAATTCACATACA
TCTGTCAGCGCTTGTTAGTTTTAACATAGAGCTTCCTTCATTTGCAGGGCTTTACACGAGAAAGAAGAACGTTGTAAAGGAGGGCTAACGCGTACTCAGT
TCTTTATAGTAGCCTTTATCTGCAGTTTTGCATACTACATTTTCCCTGGATATCTCTTTTCAATGTTGACATCTTTGTCATGGATATGTTGGATCTTTCC
CAAATCTGTGTTTGCCCAACAACTAGGTTCGGGCCTAAAGGGGCTTGGAATTGGAGCTATAGGCCTTGATTGGTCCTCTATTTCGTCCTACCTTGGAAGC
CCATTAGCTAGCCCATGGTTTGCTACAGCTAATGTTGCTGCTGGATTCATATTTGTTATGTATGTTTTGACTCCAATATGTTACTGGTTAGATATCTTCA
AAGCCAAAACATTCCCAATTTTCTCTGACGAAATGTTCACTTCCAATGGCCAAGTTTACAACATATCAAGTATAATTGACTCTAATTTCCATCTTGACGT
TGATGCCTACGAGCGACAAGGACATCTTTATCTTAGTACATTCTTCGCGGTAACTTATGGAGTTGGCTTTGCTGCTCTCAGTGCTACTGTCATGCATGTT
CTCTTATTTCACGGAAGGTAAGAAATAAGAGTAGTTATACATGAAAAAAAAGAGAAAGTAATGTTATAACGAGTTTTATGGATTGCAGGGAGATATGGGA
GCAGAGCAAATCAAGCTTCAAAGATCAGAAAATGGACATACACACTAGACTAATGAGCAAGTATAACCAAGTACCTGAGTGGTGGTTTTGGTGCATTCTT
GTCGCCAACATTACACTCACTATCTTTGCTTGTGAATATTACAATGAGCAACTTCAGTTACCTTGGTGGGGAGTTATTCTAGCTTGTGTCATTGCCTTTT
TCTTTACCCTTCCTATAGGAATCATCACCGCCATTACCAACCAGGTAACAAGGATCAATCAACTATCTGTAACAGAAAAGTCACTTAATTACTTATATAT
ATGATTTTTGAACATTGCAGACACCAGGGTTGAATGTCATCACGGAATATATCATAGGATACCTTTATCCAGGATATCCGGTCGCTAACATGTGCTTCAA
GGTCTATGGTTACATTAGTATGACTCAAGCTATAACTTTCCTCCAAGACTTCAAGCTTGGCCATTACATGAAAATTCCTCCAAGAATAATGTTTATGGCT
CAGGTAAGCTACTCGCTATAATGGATAATATAGATGATTCTCTTACGTATTTACAATAGTAGAAAATGGACACTGCAGGTAGTAGGTACTCTAATAGCAG
GGTTTGTGTATTTGGGAACAGCGTGGTGGTTAATGGAAACGATTCCAGACATATGCGACAAGACGTTGTCCAACACGGTGTGGACTTGTCCTTCGGATCA
TGTGTTTTATGATGCATCAGTGATTTGGGGTTTGATTGCACCAAGAAGGATTTTCGGAGACTTGGGAACTTATGGGATGGTGAATTGGTTCTTCTTATTT
GGCGCGATAGCACCGGTACTTGTGTGGTTAGCAGCGAGGGCATTTCCTAAGCAAGAATGGATCAAACTGATTAACATGCCAGTGCTAATAGGAGCAACAG
GGATGATGCCACCAGCCACTGCTGTGAACTACACAACCTGGATCATCGTTGGGTTCTTGTCTGGCTTTGTTGTTTATCGATATAGACCAGATTGGTGGCA
ACGACATAACTACGTTCTTTCGGGTGCACTTGATGCTGGTTTGGCATTTATGGCAGTTCTTATATATCTGTGTTTGGGCTTGGAGAACATAACTATTAAC
TGGTGGGGAAATGATCTTGATGGATGTCCTTATGCTGCTTGTCCAACAGCTAGAGGCATTGTTATAGAGGGTTGCCCTGTTGTTTATTGAATTGTGAATA
TCAACCATAAATTTGCATTTGTGTAAATCCCATTTTCTTTAAAACAGTACATCATTGGTTGTTGAGTCAACTTCAGTTGGAGAATTTTATCGAGAATTCG
TCAAAGTAGCTGCAGCTAAAGCTATAGAGTTCATAAAAC
The mutation and phenotype for selected feature: Solyc08g082990
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-1 (T0 ) | -1 bp (49.5%) / -3 bp (47.9%) | |
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-1-4 (T1 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: AAAGAACGTAGTTATGTCGTTGCCACCAATCTGGTCTATATCGATAAACAACAAAGCCAGACAAGAACCCAACGATGATC CAGGTTGTGTAGTTCACAGCAGTGGCTGGTGGCATCATCCCTGTTGCTCCTATTAGCACTGGCATGTTAATCAGTTTGAT CCATTCTTGCTTAGGAAATGCCCTCGCTGCTAACCACACAATACCGGTGCTATCGCGCCAAATAAGAAGAACCAATTCAC CATCCCATAAGTTCCCAAGTCTCCGAAAATCCTTCTTGGTGCAATCAAACCCCAAATCACTGATGCATCATAAAACACAT GATCCGAAGGACAAGTCCACACCGTGTTGGACAACGTCTTGTCGCATATGTCTGGAATCGTTTCCATTAACCACCACGCT GTTCCCAAATACACAAACCCTGCTATTAGAGTACCTACTACCTGCAGTGTCCATTTTCTACTATTGTAAATACGTAAGAG AATCATCTATATTATCCATTATAGCGAGTAGCTTACCTGAGCCATAAACATTATTCTTGGAGGAATTTTCATGTAATGGC CAAGCTTGAAGTCTTGGAGGAAAGTTATAGCTTGAGTCATACTAATGTAACCATAGACCTTGAAGCACATGTTAGCGACC GGATATCCTGGATAAAGGTATCCTATGATATATTCCGTGATGACATTCAACCCTGGTGTCTGCAATGTTCAAAAATCATA TATATAAGTAATTAAGTGACTTTTCTGTTACAGATAGTTGATTGATCCTTGTTACCTGGTTGGTAATGGCGGTGATGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Low germination (~10%); retarded growth |
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-1-4-1 (T2 ) |
-1 bp/-1 bp
Is tDNA present: Yes
Mutation sequence: AAAGAACGTAGTTATGTCGTTGCCACCAATCTGGTCTATATCGATAAACAACAAAGCCAGACAAGAACCCAACGATGATC CAGGTTGTGTAGTTCACAGCAGTGGCTGGTGGCATCATCCCTGTTGCTCCTATTAGCACTGGCATGTTAATCAGTTTGAT CCATTCTTGCTTAGGAAATGCCCTCGCTGCTAACCACACAATACCGGTGCTATCGCGCCAAATAAGAAGAACCAATTCAC CATCCCATAAGTTCCCAAGTCTCCGAAAATCCTTCTTGGTGCAATCAAACCCCAAATCACTGATGCATCATAAAACACAT GATCCGAAGGACAAGTCCACACCGTGTTGGACAACGTCTTGTCGCATATGTCTGGAATCGTTTCCATTAACCACCACGCT GTTCCCAAATACACAAACCCTGCTATTAGAGTACCTACTACCTGCAGTGTCCATTTTCTACTATTGTAAATACGTAAGAG AATCATCTATATTATCCATTATAGCGAGTAGCTTACCTGAGCCATAAACATTATTCTTGGAGGAATTTTCATGTAATGGC CAAGCTTGAAGTCTTGGAGGAAAGTTATAGCTTGAGTCATACTAATGTAACCATAGACCTTGAAGCACATGTTAGCGACC GGATATCCTGGATAAAGGTATCCTATGATATATTCCGTGATGACATTCAACCCTGGTGTCTGCAATGTTCAAAAATCATA TATATAAGTAATTAAGTGACTTTTCTGTTACAGATAGTTGATTGATCCTTGTTACCTGGTTGGTAATGGCGGTGATGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-22 (T0 ) | -4 bp (47.7%) / wt (50.1%) | |
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-51 (T0 ) | wt (66.7%) / -2 bp (31.2%) | |
| CRT0138 | RG-PtoR | CTG0138-27 | CGR0138-27 | CRT0138-55 (T0 ) | -5 bp (50.3%) / -3 bp (1.7%) / -1 bp (4.6%) / wt (31.1%) / +1 bp (5.4%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
