Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
---|---|---|---|---|---|---|
CRT0001 | Moneymaker | CTG0001-1 | CGR0001-1 | CRT0001-9 (T0 ) | -10 bp (40%); -1 bp (35.9%) | |
CRT0001 | Moneymaker | CTG0001-1 | CGR0001-1 | CRT0001-9-1 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: TAGTTGCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAAC TCCCTCAGTATCACTGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACTGGACGCAGTT AGGTTGTTCCTGAACACACATTGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTG GATCAGCCATCTGTGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGA ACTTGGTTCTATAAAATTGGTTTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCT ATAGTTTCTTGGCCTTCGATTTTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGT GTTCTATTACTACCTGTTTTCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGAT TGTTTTTCTTTTGAGCCAGGGGTCTATCGGAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-16-4 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: TTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAACTCCCTCAGTATCAC TGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACTGGACGCATGTTAGGTTGTTCCTGA ACACACATTGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGATCAGCCATCTG TGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGAACTTGGTTCTATA AAATTGGTTTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCTATAGTTTCTTGGC CTTCGATTTTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGTGTTCTATTACTAC CTGTTTTCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGATTGTTTTTCTTTTG AGCCAGGGGTCTATCGGAAACC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Stunted growth; Germination <30%(Phenotype Image ) |
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-23 (T0 ) | -17 bp (48.3 %); WT (35.3%) | |
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-23-4 (T1 ) |
-17 bp/-17 bp
Is tDNA present: Yes
Mutation sequence: GCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAACTCCCT CAGTATCACTGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGTTAGGTTGTTCCTGAACACACAT TGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGATCAGCCATCTGTGAGAATT TTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGAACTTGGTTCTATAAAATTGGT TTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCTATAGTTTCTTGGCCTTCGATT TTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGTGTTCTATTACTACCTGTTTTC TTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGATTGTTTTTCTTTTGAGCCAGGG GTCTATCGGAAACCTCTCTACCTCTGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Stunted growth; Germination >90%(Phenotype Image ) |
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-32 (T0 ) | -1 bp (65%); WT (30%) | |
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-32-3 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: GTTGCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAACTC CCTCAGTATCACTGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACTGGACGCAGTTAG GTTGTTCCTGAACACACATTGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTGGA TCAGCCATCTGTGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGAAC TTGGTTCTATAAAATTGGTTTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCTAT AGTTTCTTGGCCTTCGATTTTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGTGT TCTATTACTACCTGTTTTCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGATTG TTTTTCTTTTGAGCCAGGGGTCTATCGGAAACCTCTCTAC Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0002 | RG-prf3 | CTG0001-1 | CGR0001-1 | CRT0002-32-5 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: TAGTTGCCACCCTTTTGTCTCTTATCAGCATCACGTCTCGTCCTAGGTGGAGTAGCAGGTGTCCAGCGTGGGTTTTGAAC TCCCTCAGTATCACTGTCAAATTTCTGTGCAGCAAGGTAAGATAAGGCTGTGACAACGTCTGCTATGACTGGACGCAGTT AGGTTGTTCCTGAACACACATTGCTGCAACTGCTAGAGCTTGGTATAAGCCTCTTTCTGGATATTGTCCTTGAAGCATTG GATCAGCCATCTGTGAGAATTTTCTCCGGTCTCTGAACAACGGTTTTGCCTGGAAAGGAGAGCATGAGTTTAATATTTGA ACTTGGTTCTATAAAATTGGTTTTCTAATAGCAAAATAATGTTGTTTTCCATTCATATTAGTAGTCATAATATTGCCTCT ATAGTTTCTTGGCCTTCGATTTTTCTTAATATCTATTGTCCTTGTACCATAATTATCGAATTTGTTTTGATGTAGTTTGT GTTCTATTACTACCTGTTTTCTTCTTATTATATGCTGTTTATTGTACTTCCATTACTTCTTGTTTGGGACTGCTTTGGAT TGTTTTTCTTTTGAGCCAGGGGTCTATCGGAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-1 (T0 ) | -7 bp (28.5%); -1 bp (47.2%); WT (12.4%) | |
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-1-1 (T1 ) | -1 bp /-7 bp | |
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-1-2 (T1 ) | -1 bp /-7 bp | |
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-2 (T0 ) | -2 bp (13.6%); -1 bp (60%); WT (22.8%) | |
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-2-5 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: TTCAACCAAAATCTGGTGGATTGGGGGCATTACCAGATGGAGACCGAAAAAAGAACTTCATTCCATTATTGAAAAGAAAA AAGTGACAGAAAAGACCTGATTGGAATCGAGCCGCCCTTTATATACTCTTCCGAATCCTCCCTCTCCAAGAAGACAATCA CCTCGAAAGTTTCTTGTTGCAACTGCTAATTCACGAAATTTAAAAATCTTGGCTCCAATTTGACCAGACTCGCCATCCTT AACATACTCCTTTGAACCTAACAGAGTCTTTGCCTTCAACTTATCTGAACACGAAAAAAAGAGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0075 | RG-PtoR | CTG0075-1 | CGR0075-1 | CRT0075-2-6 (T1 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: CTATGCGACAAAATAGCAAGTTCAACCAAAATCTGGTGGATTGGGGGCATTACCAGATGGAGACCGAAAAAAGAACTTCA TTCCATTATTGAAAAGAAAAAAGTGACAGAAAAGACCTGATTGGAATCGAGCCGCCCTTTATATACTCTTCCGAATCCTC CCTCTCCAAGAAGACAATCACCTCGAAAGTTTCTTGTTGCAACTGCTAATTCACGAAATTTAAAAATCTTGGCTCCAATT -GACCAGACTCGCCATCCTTAACATACTCCTTTGAACCTAACAGAGTCTTTGCCTTCAACTTATCTGAACACGAAAAAAA GAGAAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.