Name: Solyc09g014720
(Exon Intron)
Length: 3506
TCAAATTGCCCTTCTGTTTTTCATCAATGGGCTATTTGTGTTAGAAGTACTACCTGAACTATATTGTCCAGAAAAGTTGCTGCTATTTGTATAGGAATTAGAACTATATTGTCCAGAGAAATTGTCGATGTCTGTATTAGACTCGATTGGAATTGTATAAAGATCTGATAATTCATCTTCGTTCTCTTCATGTCCGTTTC
CATTAGCCCATGGGTTATTTTTGGTGAACTTTCTCAAATTTTCAAGTTCACTTGCTACTTCTTTCATGGTAGGCCTATCTTCTCCGTGCAAACTAAGACA
GCTCTTCACTAGCTCAGCCACCTTTTGGAGTTGTTCGAGGCTTCCCTCTCTTACAACTCTACGATCAAGAATTTGAAACAATTGATTCCTTCTCATCGAC
AAAACAAAATACTCTGCCAAATTCTTCTCCTCCTCATTTCTGTCCTTGGAGATAGGTTTCATCCCTGTCAATAGTTCGGCAAGAACTACTCCAAAACTAT
AAACATCACTTTTCTCAGTCAATTGACTTGTCCGAAAGTATTCAGGATCTAGGTATCCTAATGTCCCTTGAACCATTGTAGCAAGATGTGTTTGATCAAT
AGGGATCAACCTTGAAGCACCAAAATCTGCCACTTTCGCGATGTAAACATCATCCAACAATAAATTGGCAGACTTGACATCTCTATGAATTATAGGCATT
TGCGCGGATGAATGAAGGTAAGCAAGTGCACTTGCTGTCTCACTAGCAACTCTTAGCCGATTTTCCCAAGATAACCAAGGTGCTCCATTTCGATTGTGGA
TATGCTCGTAAAGAGTTCCATGAGAAATGTATTCATAGACAAGTAAAGGAACTTCGGCTTCCAAACAACATCCGAAGAGTCTCACAACATTTCTATGGTT
GACTTGAGTAAGAATAAGTACCTCGTTAATGAACTGTTCAACCTGTTCCTCGTCCATAAACTTAGATTTCTTAATAGCAACTATGCGATTATCAGATAGA
ATGCCTTTGTAGACAATTCCATTTCCACCACGACCAAGAATTCTATCACTGGCATAGTTGTTTGTAGCCTTCTTCAGCTCTGCAGCAGTGAAAATTTTGG
TTGCTTCAACACCACCCTCGTTAGAAGACATTCTTTGTTTCATAAGCAAACCACCATTTTGTTGGAAGAATTTCTCGCGAAGTTTAATGAGTTTTCTTTT
TTTGATGAAAAAATAGAGCCAAGTTGTCCCAATTACTAGTGACATAAAACCAACTCCCATACCTGTGTTTATGGAACGAATTTATTGGAATTAGAAATAT
AAGAGAAGGGACATATGAGAGTAGAGACAAGTTGAAATATCTTAGAAACTAGGGGCGTTAAAAATGAATCCAAACATAGATAACCCGTCCAATCCGTCTA
GAATTTTAAGGGTAGGGCTCAACATAATTTGAATTGGTTTCATTGTCAACCAGTGGCGGAGCCATTCTTCGAAAAATTATATTGTGTAGCTAGAATAAAA
ATTATTTTTTATGTATATATATTACATATTGACACTCCTTGGCTTCTACACCATCTTATTTCTTAATATTTTGACACCCCTTAAATAAAATTCTAGTTCC
GTCACTGTTCTCAACTCATTTAAGCTTAACCCATTTGAAGAGAATTCTTAATTGAGCCAAATTTAATCTCCAATTTCAAACCGTTTTAAAATTTTTTACT
AAGATATGTTCCTATATTGAAGGTATGAATTATTATTTATTCAACATCTTTTAGAATTTATCTATCCATTTGTTACTTTTTTAACAAAAAAATCTTGATC
GAGAATTCAAATTGTGATTATAAAAGTTAAATATTAATTTGTTAAATTATTGAGATTAATCGGGTCAAATTACGCGGATCAAGACCCAACCCATTTTTTT
AATTCATTTCAGCCCAACCCATTTGAGGGCAAAGTAAAGTTGGACAAATCAAGACCCAACTCAATTTCTATTGAACCCGTTTGAATATTTTCATTTTTAA
CCCAACCCCCCATTAGACACCCCTGATAGAAACTTACCAACAGAAAACTTGATCCATGGGAACTCTGAGTTCGAATTTGGTGCAATACAACCACGGCCAT
TCTTTCTGCCATCACCGGTGTATCCTTCTGGACAAGAACAATTGTAACTCCCAGGTATGTTTGTGCAAATCTTTTCACATGAATTGGTATTTGGATCCGC
ACATTCATCGATATCTAACATGAAAAACAGAGAAAATTAATCCCAGACTCATTGTCCTTTTTATCAATTTTTATTTTAGATACTTACTACTAAAGTTGGA
ATTAAAGAATTTTTATAAAATGAAACAGACATTATTTTTTAAACAGACTAAAAGAGAATGTATAATAAACAAATTGAATCAGAGGGAGAAAAAGAGACAA
GGTACCTTGGCAGCCAGAGCCGATGTATGGATTGCCTATATATCCCGAGTTACAGCTACACCGATAGCCACCAAGGCTAGTGTCGGAATCAACACATTGG
CTATTATTGAGGCAAGCATAATCGTTACTTTTTCGTGCTTCAACACAAGAAAGATTTCCAATAGCCCAATCTAACACAATAGGCACATTATTCCTAATCT
TGTTTGCAAAATTAACATCACTAAGATCCTCTATACCCTGAAACTCGAATCGACTAGCTTCACCAAGAAACGAATGTCCACATGGATTAAACGACCAAAT
TAAGCTATGGTTTCTTGTGGTTACCATAGTTGTATTGAAAAATTTCAAGCCTTTTGGTATTGTAATTTGACAGCAACCAAAACCCATACATTTTCCTTTA
ACTATATCATTGGTGCTCGTGCAGAGAGAGGGGCAACCATTAGCAAAGTTATTTCCACTCACTATAGCACCGTCATCACAACCAACCACGATGAATCTGC
AGAGGCAAATTTACAATTTAGAGTCAGTAAGTTTCGGTGACTTGACCGAACAAATTGAACTGCAATCCAAATATAAATATAAATATATGATTGAAGCAGA
AAATAATAAGTTCGGTTTGAATTGAATCGACAGTACCTATTGAGGGTAGAAAAAGTGTAAGGACTTTTTGTTCCTAAAGTTATCCAAGAAGGAATATCTT
GAATTAAAACTCCTGTTTGTGAGTAACACTTTGTATTTATAAAATTTGATATACGCATTTCAGCGTCTGAGATATCGTAAATTTGAATATTCCCTATGAA
TGGTGTTGTAACGTTGCAGTCGATCTCAAACATGGGGTCTAAGGCACAACCAGACCCAATTCCAAATGGATATGGAACTGTAACGTTTCCACACTGTTTT
GGACATCCTGGTTTTGTGATGTTAGCAGCTTTGGTGATTGTGTTGGTGGGGGAGGGGGCGGGGGCGGTGGCATTCGTTGGCGGAGAAGTGGAGTTTGTGG
GCGGAGATGTGGTGTTAGAAGGGATAATTTGGGCGGTGGCTAGTGTTAAGATTAGCATAAAACAGGGGAGTTGGAAAGAAAACAGAGCAACTTGATAATG
CTGCAT
The mutation and phenotype for selected feature: Solyc09g014720
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4 (T0 ) | -1 bp (41.5%); -10 bp (52.4%) | |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4-4 (T1 ) | -1 bp/-10 bp | Immunity response: More susceptible to DC3000dd |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4-4-4 (T2 ) |
-10 bp/-10 bp
Is tDNA present: No
Mutation sequence: ATATATCTAAGTTTAGTCCGTCAATTAGAGTTAAGTTTTTTTTCAGGTTGTCAGTGATTCGATACGAAACTAATAATTTG CTTCAAATTCAGAAAGTGTTTTCAACAAAATCTCTCTAAATAAAACTACAAATAGGAAATAATCCAAAGATACTATTGTA CGAATCGGTTCCCCTTAAGCCCTATATATGGAACCTTTTACTCAATAAGTCCTTTTAATTTCCATAATAATATTTTTTCA AAACCATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTCTTAACACTAGCCACCGCCCAAATTATCC CTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAAC ACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGG AATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000dd |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4-9 (T1 ) | -1 bp/-10 bp | Immunity response: More susceptible to DC3000dd |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4-9-3 (T2 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: GAAGATATATCTAAGTTTAGTCCGTCAATTAGAGTTAAGTTTTTTTTCAGGTTGTCAGTGATTCGATACGAAACTAATAA TTTGCTTCAAATTCAGAAAGTGTTTTCAACAAAATCTCTCTAAATAAAACTACAAATAGGAAATAATCCAAAGATACTAT TGTACGAATCGGTTCCCCTTAAGCCCTATATATGGAACCTTTTACTCAATAAGTCCTTTTAATTTCCATAATAATATTTT TTCAAAACCATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTTATGCTAATCTTAACACTAGCCACC GCCCAAATTATCCCTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCC CTCCCCCACCAACACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTC CATATCCATTTGGAATTGGGTCTGGTTGTGCCTTAGACCCCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000dd |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-4-9-7 (T2 ) |
-10 bp/-10 bp
Is tDNA present: No
Mutation sequence: ATATATCTAAGTTTAGTCCGTCAATTAGAGTTAAGTTTTTTTTCAGGTTGTCAGTGATTCGATACGAAACTAATAATTTG CTTCAAATTCAGAAAGTGTTTTCAACAAAATCTCTCTAAATAAAACTACAAATAGGAAATAATCCAAAGATACTATTGTA CGAATCGGTTCCCCTTAAGCCCTATATATGGAACCTTTTACTCAATAAGTCCTTTTAATTTCCATAATAATATTTTTTCA AAACCATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTCTTAACACTAGCCACCGCCCAAATTATCC CTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAAC ACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGG AATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000dd |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-5 (T0 ) | -2 bp | |
| CRT0016 | RG-PtoR | CTG0016-1 | CGR0016-1 | CRT0016-5-1 (T1 ) | -2 bp |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
