Plant Crispr Database

Feature Information

Feature ID: Solyc09g075820
Feature Type: gene
Name: STP13
Description: Solute carrier family 2, facilitated glucose transporter member 3
Links to other Database: TFGD, TEA, SGN, iTAK

Sequence & Structure:

The mutation and phenotype for selected feature: Solyc09g075820

Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)

Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu

Transformation	Genetic background	Construct	gRNA	Plant	Mutation	Phenotype
CRT0030	RG-PtoR	CTG0030-2	CGR0030-2	CRT0030-2 (T0 )	-1 bp/-1 bp Is tDNA present: Yes Mutation sequence: ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGAAACCAACGCCACAACCAAGGAGAATTCTTC CAATAATAAGCATAGCTAAATCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGCGATTAACATA GTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAAAAAACGTCGCCGTTAACCAGCTAGGTATAACGATGAAGTA AATAATTGTAACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTTTGTTCTCTTATAAACTGTTGG AAAGAATTTTTTTAAAAATGGATCCATTGATGTAACACCACCTGTATTTTTTTAAAAAAAAAAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4633 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA 4682 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA 50 Wildtype 4683 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA 4732 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA 100 Wildtype 4733 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC 4782 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC 150 Wildtype 4783 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA 4832 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA 200 Wildtype 4833 AAAACGTCGCCGTTAAACCAGCTAGGTATAACGATGAAGTAAATAATTGT 4882 \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 AAAACGTCGCCGTT-AACCAGCTAGGTATAACGATGAAGTAAATAATTGT 249 gRNA TTAAACCAGCTAGGTATAAC Wildtype 4883 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT 4932 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT 299 Wildtype 4933 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG 4982 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG 349 Wildtype 4983 ATGTAACACCACCTGTATTTTTTTAAAAAAAAAGA 5017 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|.\| Mutation 350 ATGTAACACCACCTGTATTTTTTTAAAAAAAAAAA 384
CRT0030	RG-PtoR	CTG0030-2	CGR0030-2	CRT0030-2-4 (T1 )	-1 bp/-1 bp Is tDNA present: No Mutation sequence: ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGAAACCAACGCCACAACCAAGGAGAATTCTTC CAATAATAAGCATAGCTAAATCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGCGATTAACATA GTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAAAAAACGTCGCCGTTAACCAGCTAGGTATAACGATGAAGTA AATAATTGTAACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTTTGTTCTCTTATAAACTGTTGG AAAGAATTTTTTTAAAAATGGATCCATTGATGTAACACCACCTGTATTTTT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon Wildtype 4633 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA 4682 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 1 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA 50 Wildtype 4683 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA 4732 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 51 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA 100 Wildtype 4733 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC 4782 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 101 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC 150 Wildtype 4783 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA 4832 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 151 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA 200 Wildtype 4833 AAAACGTCGCCGTTAAACCAGCTAGGTATAACGATGAAGTAAATAATTGT 4882 \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 201 AAAACGTCGCCGTT-AACCAGCTAGGTATAACGATGAAGTAAATAATTGT 249 gRNA TTAAACCAGCTAGGTATAAC Wildtype 4883 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT 4932 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 250 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT 299 Wildtype 4933 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG 4982 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 300 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG 349 Wildtype 4983 ATGTAACACCACCTGTATTTTT 5004 \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\| Mutation 350 ATGTAACACCACCTGTATTTTT 371

Transformation

Genetic background

Construct

gRNA

Plant

Mutation

Phenotype

CRT0030

RG-PtoR

CTG0030-2

CGR0030-2

CRT0030-2 (T0 )

-1 bp/-1 bp

Is tDNA present: Yes

Mutation sequence:

ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGAAACCAACGCCACAACCAAGGAGAATTCTTC
CAATAATAAGCATAGCTAAATCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGCGATTAACATA
GTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAAAAAACGTCGCCGTTAACCAGCTAGGTATAACGATGAAGTA
AATAATTGTAACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTTTGTTCTCTTATAAACTGTTGG
AAAGAATTTTTTTAAAAATGGATCCATTGATGTAACACCACCTGTATTTTTTTAAAAAAAAAAA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        4633 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA   4682
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation           1 ACGTATTTATTATAGCAGTCAAAAAATATTCAGACGAACCTGATTGGCGA     50

Wildtype        4683 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA   4732
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation          51 AACCAACGCCACAACCAAGGAGAATTCTTCCAATAATAAGCATAGCTAAA    100

Wildtype        4733 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC   4782
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         101 TCTTGAGCAGCAGCATTTAGTACAACTCCAATAATGAAAAAACAACCGGC    150

Wildtype        4783 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA   4832
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         151 GATTAACATAGTTAATCTCCGGCCAAGTTTTCTTGTAGTGTAAGAAGCAA    200

Wildtype        4833 AAAACGTCGCCGTTAAACCAGCTAGGTATAACGATGAAGTAAATAATTGT   4882
                     |||||||||||||| |||||||||||||||||||||||||||||||||||
Mutation         201 AAAACGTCGCCGTT-AACCAGCTAGGTATAACGATGAAGTAAATAATTGT    249
gRNA                             TTAAACCAGCTAGGTATAAC

Wildtype        4883 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT   4932
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         250 AACCCTTGATTATCGTATTTACAGTAATTACTGTCTAATCCTGGCTCCTT    299

Wildtype        4933 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG   4982
                     ||||||||||||||||||||||||||||||||||||||||||||||||||
Mutation         300 TGTTCTCTTATAAACTGTTGGAAAGAATTTTTTTAAAAATGGATCCATTG    349

Wildtype        4983 ATGTAACACCACCTGTATTTTTTTAAAAAAAAAGA   5017
                     |||||||||||||||||||||||||||||||||.|
Mutation         350 ATGTAACACCACCTGTATTTTTTTAAAAAAAAAAA    384