Name: Solyc10g011910
(Exon Intron)
Length: 1942
TCTGCCACGTGTCCTTTATCAGTAGTAGAGCAATCAACGACCGCCTGCCGCCGTAGTTGCTGCGTTATTCGCCAGCCAAGGGAACTGAAAATTCGCCGGAAAATTCTCCGGCGAGATATCTCCGGTGGCCGGGATTTCCAATTCATCCAAACCGTCGAAGAAATCATCTTCCGGTTCCATTTTATCCAACCCAACATCGG
AAACACCAAATTCATCATCGTCATCTTCAAAAGTATCTTCTTTTTCGTCTCTGCTGCTTTCTTGATTTTCCGGTGCCGGTGAATTTGTCACCGGCGAAGA
TACCGGCGACGATCCGGTGGGTTTCACCGTTTTTTGGCGGCTGATTCCGGCTAAGGAATTTCTGTGAGTAGGCATTGGATGGTTGTGCTCAGCTGTATAA
GTGACAATAAACATATTCGGGTCGGATCTATTCCGCTCCACTTGTTTTCGGGCCAAACAACCTTTTGAAGTGCTACATTTGTAATATCCCCTGTTTATAA
TCAAAATAAATGTCCATAAATATCCATCGAAATTTCACAAAATACAACTAAGATAGTGTATAATAGATTCTTTTATTTTGATTTCATAAATATCCATCGA
AATTTCACAAAATACAATTAAGATAATGTATAATAGATTCTTTTATTTCGATTATATCCTCCGAATTATATATAGTAAGAGTCAAATTTTTCTAGTGAAC
AAGAAAATTTTCAATATATTGAGTTGAGTTTATTCTTGTTCTTTTTTTTTTTAAACAATGAAAAAATTTGTTGGAAACAACCAAATATGTACTCATTTAA
TTTCCTTCACTTTATTAGTTAGTTTTCAAAAGTTAATTTAATTACTTTTGAAAATTTATATTAATTTGATACTTTAAATAAAATAAAAATATATATAATA
AAATATTAGTTAATTTTTTTATAATTTGACTTTAAAAAATTAAAAATATGTCAGTTAAAATTAGAGGGAAATAGTTGTAAAAGAAACCAATAATAATACC
TTGGGTATGGGGAACCTTTAATAGGTTTTTGTCCATATTTTCTCCAAGACCACATATCAGAAGATAAACCATCAGCAGCTACTTGACATACTTTCTTTAA
ATGGTTCTTCCTGTAAATAATAAAAATAAAATCCATCAAGTAAATTAAAAAAAAATAACATTGAGGTGTAAAAATTGAAAATTTTAAAAAAATTGTTGCA
TCTTTTATCCTCAACCCAAACAAATTAAAATCATCCAACAAAAAAAAAACATCAAATTTTCTCACTATATAAGAATTTTCACCATATAATAAAAAGTGAA
CGTAGCTTGATTTCATAAGGTTTATGTACAGATCATAAATTTATATGTTTACAGTAGTAAATATAAACACATAAATAATATTGTTTTTACGATATTAATT
CAAATGAATATCCATAAATTGTAGATCTGTTGTGTAAAAAGTAATAGTGATTATAATTTACCTTCTTTTGATTGTTTGACTTTGAGCATGTAAATTACAT
GATAAAGTTCTGTTGGGTTGAATTGATTTCGTATCAGTAAGCTGTTGCGACGGTGATTGTGATGAAATGACCGTTAATTTTTCGGATGGTTGATTAATAA
ATGGCTTGCAAAGTTGATGCAATTCTTCGAAATCATCACTAATTTTATTTCTATTCAATACTAGGCTCTGTTTGAGAACCAGATCTTCAAAGCAAAATAC
AGAGTTATTGTTACTGTTATTATCATCATCTGTTGTTCTTGGTTGGAAGTTGGTAAAACTGATGTCGCCGGCGGTTGTGGTGGCGGTGCAGCTTCTGACG
ACGGCGTTTAGATCCCAATCTTCTTCCATAGTCTCTCTCTGTTTTTGCTCTGCTTTATCCTTGACTTTTTCTTTTCTTTTTTTAGAGAGAAGAAGAAGAC
AAAAGAAGGCGCTGACTTTTTTGAGGTAAAGAGTGAGGGGAT
The mutation and phenotype for selected feature: Solyc10g011910
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-13 (T0 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: CAAAAACCAGCTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCC CCTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGG ATAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACC ACAACCGCCGCGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTG TATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTG CATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCAACAGCT TACTGATACGAAATCAATTCAACCCAACAGAACTTTATCATGTAATTTACATGCTCAAAGTCAAACAATCAAAAGAAGGT AAATTATAATCACTATTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Germination <25%; Most of the germinated seedlings don't have meristem(Phenotype Image ) |
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-13-7 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: CAAAAACCAGCTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCC CCTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGG ATAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACC ACAACCGCCGCGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTG TATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTG CATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCAACAGCT TACTGATACGAAATCAATTCAACCCAACAGAACTTTATCATGTAATTTACATGCTCAAAGTCAAACAATCAAAAGAAGGT AAATTATAATCACTATTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-13-8 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: CAAAAACCAGCTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCC CCTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGG ATAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACC ACAACCGCCGCGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTG TATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTG CATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCAACAGCT TACTGATACGAAATCAATTCAACCCAACAGAACTTTATCATGTAATTTACATGCTCAAAGTCAAACAATCAAAAGAAGGT AAATTATAATCACTATTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Germination ~30%; Most germinated seedlings don't have meristems; Short stature(Phenotype Image ) |
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-25 (T0 ) |
azygous
Is tDNA present: Yes
Mutation sequence: TATTTTTTAGGATATTTTATTTTTAACATTCTTTAAAAATTAGTCATCAAAAACCAGCTAAAAATACACGCATACAACAC TCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCCCCTCACTCTTTACCTCAAAAAAGTCAGCGCCTT CTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGGATAAAGCAGAGCAAAAACAGAGAGAGACTATGG AAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACCACAACCGCCGGCGACATCAGTTTTACCAACTTC CAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTGTATTTTGCTTTGAAGATCTGGTTCTCAAACAGAG CCTAGTATTGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Germination <50%; Most of the germinated seedlings don't have meristem(Phenotype Image ) |
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-25-12 (T1 ) |
azygous
Is tDNA present: Yes
Mutation sequence: TATTTTTTAGGATATTTTATTTTTAACATTCTTTAAAAATTAGTCATCAAAAACCAGCTAAAAATACACGCATACAACAC TCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCCCCTCACTCTTTACCTCAAAAAAGTCAGCGCCTT CTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGGATAAAGCAGAGCAAAAACAGAGAGAGACTATGG AAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACCACAACCGCCGGCGACATCAGTTTTACCAACTTC CAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTGTATTTTGCTTTGAAGATCTGGTTCTCAAACAGAG CCTAGTATTGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-25-7 (T1 ) |
azygous
Is tDNA present: Yes
Mutation sequence: TATTTTTTAGGATATTTTATTTTTAACATTCTTTAAAAATTAGTCATCAAAAACCAGCTAAAAATACACGCATACAACAC TCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCCCCTCACTCTTTACCTCAAAAAAGTCAGCGCCTT CTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGGATAAAGCAGAGCAAAAACAGAGAGAGACTATGG AAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACCACAACCGCCGGCGACATCAGTTTTACCAACTTC CAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTGTATTTTGCTTTGAAGATCTGGTTCTCAAACAGAG CCTAGTATTGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-26 (T0 ) |
+1 bp/+1 bp
Is tDNA present: Yes
Mutation sequence: AAAACCAGCGTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCCC CTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGGA TAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACCA CAACCGCCGAGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTGT ATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTGC ATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Germination <50%; Most of the germinated seedlings don't have meristem(Phenotype Image ) |
| CRT0007 | RG-PtoR | CTG0007-3 | CGR0007-3 | CRT0007-26-3 (T1 ) |
+1 bp/+1 bp
Is tDNA present: No
Mutation sequence: AAAACCAGCGTAAAAATACACGCATACAACACTCTCTTTCTCTCTCTCTATATGATCCCATCCCACCCATTTTACATCCC CTCACTCTTTACCTCAAAAAAGTCAGCGCCTTCTTTTGTCTTCTTCTTCTCTCTAAAAAAAGAAAAGAAAAAGTCAAGGA TAAAGCAGAGCAAAAACAGAGAGAGACTATGGAAGAAGATTGGGATCTAAACGCCGTCGTCAGAAGCTGCACCGCCACCA CAACCGCCGAGCGACATCAGTTTTACCAACTTCCAACCAAGAACAACAGATGATGATAATAACAGTAACAATAACTCTGT ATTTTGCTTTGAAGATCTGGTTCTCAAACAGAGCCTAGTATTGAATAGAAATAAAATTAGTGATGATTTCGAAGAATTGC ATCAACTTTGCAAGCCATTTATTAATCAACCATCCGAAAAATTAACGGTCATTTCATCACAATCACCGTCGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
