Name: Solyc10g047320
(Exon Intron)
Length: 3116
TCAGAGATCAGGAGGGAATATGGAAAGCTTGAAGGCAATGTTTTTAGTCCCTTGTTGAGTTTGATTGTCTTTTTTGGCTTGTATTTGACGTGCAGATATTGCTGCTGTTTTGGTGGAATTTTGCAACATCATGACTTGGAAACTGCGTATATCAGCTAGGGCGATGGGGACTTCCTTAAGATTATCACAGCAGATGAGAA
CGAGATGCTTAAGTCTTGGAAAATCATCAGCTGATGCTTTCCATGAAACTAAGTTTGTCCTTTCAATCCACAACACCAGTAGGGAACAAAAACCACCAGT
AGACTCCCAGGACTCTCCCGAAAACCCATTTTCTTTCATCTTCAGGACTTCAAGGTGTTCCAACTGACCCAATATAGACATATCTTTCCACTCCAACCAC
GTGTCTAATAAAGTCAGCTTCCTCAACTTTGTTGGAAATATGTATGCAGGTGGAAGGCGTAACTTACTTGTTTGAATGCTACTATCATTTATCAGCTTCA
AGTTTTCAAGGTATTCTAGCCTCTTCACATTTTTTAACGACGCAGCAGACTTATTATCAAGAAGCACTGATATTTTCCCACGGATGCCCAGCTTTTTCAG
GTTTGGAGTCCTCGCAAACACTTCCTCGGTGCAGCTTTCGGGAGCTATGGTGGAGAGAGTTTGCAGGCTTTGATTTACCAATGTAACCTTACTATTTTTT
GGAGCAACAGGGACAGGCAACTTAGCGGAAGAGTTAGTGTGCAGATGCCTTAGTCGTTCCATATTCCATATGTTTGCTTGAATATCAAGAGTGCGTTGTT
GCGTGTTAATTATGATGGTTTGGATGTTCCAAAGTTCTCCCATGAGTTTAGGAAGAATCTTGATTGAGTCAGATGAAAAAGCAACATACCTCAAATGATA
TAACTGGTAAAACTCCCTGGAAAAGCGACTGAAGTTGATTGATTCTACATCCAAAACCCTTAGCAACGGAAAGCCTTTGGGTATGGTTGGGATGTCAGCA
GATGGCATCTCAATCTTTTTCGAAGAAAAAGATAAGAATGACCTGACATGTTCAGCTGAAGGTTTCGTAGAGAAAAAATCCAAAACTGAGGAATGGATGC
ATAAGCGACGGTAGGTGGATAGTTCTCGTTTTCCAGGAAAATATTGCTCAGATCCTAACTTTATTTCTTGGAAAAGATTTTCTTCCTTCATAGCTTCTTG
CCTGCAAAACTCGTGCAACATGTCATGAAGACGACATGTTTTGATTTGACCATCAGAAGTTCTTTCCATTACCATCACTAGATTCCTATTGATGAGATCA
TTCAAGTTGTCCTCTCCTTTACACTCAAGAGATAAATGGCCTTTATACTGTATGAATCCTTCCGCAATCCACAAACGGATTAACTTCCAAGCAGGGATTT
GAAAGCCTCCAGGAAATGCACTACAATATAAAAAGCATGCTTTCAAGTCGTAAGGTAAACGATCATAACTCATTTGCACCAGTTTGTTACAATTCTCAGG
GTGGTCTCTATTTATGAGGTGTTCACTAACACTATCATCCACTTGTTTCCACTCTCTTGGTGTCTTGCCTTTCCCTATTAGTGCTCCTGCAATAACAACA
ATCGCAAGTGGTAGTCCCTTGCATTTTTTTGCTATGCTCTTTCCAGATAGTACCAATTCAGGAGGACATTTGTCCTTGTGAAAAACCTTCTTCTCCAGTA
ATATCCAACTTTCATCTTCAGTCAGAAATTTTAGATCATGAGGTATGGGATTGCATTGTTTAGCCACTTTGGAATCTCGAGTGGTCAACAATACTCGATT
CGGTTTGTTGTTATTTGGGAATGCTATCTTGATACGTTCCCAAGCTTCATCCGACCATACGTCATCCAACACAACCAAGTATTTTCCTCCCTTTCCCAAG
AATTCTTGTATTTCATCAGCCAAATCCTCCTCACACATCCCATGATATTGTTTTGTATTTCGAGTGAATTTGCTGATGATGTTGAGAAATAATTCTCTTC
TCCTGTATGATTGAGACACATAAACCCAAATACGAGTAAAAAACTCATACCCGATTTTAGGATGCTTGTAAATCTTATTTGCTAGGGTCGTTTTGCCGAG
ACCAGGCATACCAACAACTGGAACAACTTCTAGATGATTCGATTCTCCAAGAAGGCGGTTGATTACAATATCTGCTTCTTCGTCAAATCCAACCACGTCA
TCTTCCTCTACCACTGGAGGCTTTAGATTAATATAGCCAAGATAAATATTAGATATCTAAACAAGTCAATATAATATAATAGACAGATAGAAGAGTTACA
TTTATCAAGAGTGACACATACATACATACATACATGTACTCTGATTAAAGGCACTATACATCAACATATCCTATAACTTGGATTCAGATGACAACTAGCA
GATTAATCAACTTATATAAAGTCGATTGATGCATATTACATGACGTCTATCCTATATGGCGTTGTACATGTATTTTGACCACATAGGACGAGTGTGTCTA
CTAATTCAATTTTAAACTAGTTTAAGTGTCTAGTAATGCACATTCAAAGTCGAAGTCATAAATACGAGATGAAATCAAGTTAATAGGACACGATTTTATT
GATCTTTGTAACTAGATTGCTCACTAATTTATTTCCCCAATTTTTTTTCAAAAAAAAAAAGTGTTTAGGATGTGATACCTTTCTTTCTTCGGAACCTCTA
GCAGATAAATCATCATCTTGAAGGGCCTGAAGTCCAATGGCATCAGTCTGTCGGATCTCTCTGACTTTGTTTCGTATAGCTTTGATCTCACCAGCTACCT
CTTTGACTCTTTTATAATGGGGAAGATCTAATACTCTGGTGACACCCTTATCCTTGTGTAGCTTAGCTTCAATAACAAATTTATCAATAGCATCTTCAGC
AGAGTTAACGACTGTTCTTATTTTCTTAACAAGTTCTCTGAGGACTTCGTTCTCGTTGATGTGACACTTGGCAGCTTGCTTAAGAAAAGCATTGAAATCA
TTTAGATCTTGAAGTAGACTCTCAGCAGCCTCTTTAACTCCACTAATTAGCTCTACGTTGTCCCGCAGCAACTGCATCAAGTTCTCAACCAGAAATTCCA
CTGCTACGTTCGCCAT
The mutation and phenotype for selected feature: Solyc10g047320
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1 (T0 ) | WT 45%; -3 bp (50%) | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-3 (T1 ) |
-3 bp/-3 bp
Is tDNA present: No
Mutation sequence: GTTGGTATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTAC TCGTATTTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATA CAAAACAATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTG GTTGTGTTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGCGAGT ATTGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATG AAAGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCATA GCAAAAAAATGCAAGGGACTACCACTTGCGATTGTTGTTATTGCAGGAGCACTAATAGGGAAAGGCAAGACACCAAGAGA GTGGAAACAAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-6 (T1 ) | -3 (46.4%); -5 bp (50.4%) | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-6-1 (T2 ) | -5 bp/-5 bp | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-7 (T1 ) |
+1 bp /+1 bp
Is tDNA present: Yes
Mutation sequence: ACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTATTTGGGTTTATGTGTCTCA ATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAACAATATCATGGGATGTGTG AGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTGTTGGATGACGTATGGTCG GATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATNCGAGTATTGTTGACCACTCGAGATT CCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAAAGTTGGATATTACTGGAG AAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCATAGCAAAAAAATGCAAGGGACT ACCACTTGCGATTGTTGTTATTGCAGGAGCACTAATAGGGAAAGGCAAGACACCAAGAGAGTGGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-7-2 (T2 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: ACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTATTTGGGTTTATGTGTCTCA ATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAACAATATCATGGGATGTGTG AGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTGTTGGATGACGTATGGTCG GATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATNCGAGTATTGTTGACCACTCGAGATT CCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAAAGTTGGATATTACTGGAG AAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCATAGCAAAAAAATGCAAGGGACT ACCACTTGCGATTGTTGTTATTGCAGGAGCACTAATAGGGAAAGGCAAGACACCAAGAGAGTGGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-1-8 (T1 ) |
+1 bp /+1 bp
Is tDNA present: Yes
Mutation sequence: ACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTATTTGGGTTTATGTGTCTCA ATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAACAATATCATGGGATGTGTG AGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTGTTGGATGACGTATGGTCG GATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATNCGAGTATTGTTGACCACTCGAGATT CCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAAAGTTGGATATTACTGGAG AAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCATAGCAAAAAAATGCAAGGGACT ACCACTTGCGATTGTTGTTATTGCAGGAGCACTAATAGGGAAAGGCAAGACACCAAGAGAGTGGAA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-2 (T0 ) | -2 bp 44%; -12 bp 50% | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4 (T0 ) | +1 bp (53.5%); +2 bp (43.7%) | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-2 (T1 ) | +1 bp; a large overlaping | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-2-2 (T2 ) | -265 bp/-265 bp | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-2-4 (T2 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-7 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-7-3 (T2 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-8 (T1 ) | +1 bp (30%); and mulitple other deletions | |
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-8-4 (T2 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: ATGCCTGGTCTCGGCAAAACGACCCTAGCAAATAAGATTTACAAGCATCCTAAAATCGGGTATGAGTTTTTTACTCGTAT TTGGGTTTATGTGTCTCAATCATACAGGAGAAGAGAATTATTTCTCAACATCATCAGCAAATTCACTCGAAATACAAAAC AATATCATGGGATGTGTGAGGAGGATTTGGCTGATGAAATACAAGAATTCTTGGGAAAGGGAGGAAAATACTTGGTTGTG TTGGATGACGTATGGTCGGATGAAGCTTGGGAACGTATCAAGATAGCATTCCCAAATAACAACAAACCGAATTCGAGTAT TGTTGACCACTCGAGATTCCAAAGTGGCTAAACAATGCAATCCCATACCTCATGATCTAAAATTTCTGACTGAAGATGAA AGTTGGATATTACTGGAGAAGAAGGTTTTTCACAAGGACAAATGTCCTCCTGAATTGGTACTATCTGGAAAGAGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0022 | RG-PtoR | CTG0022-2 | CGR0022-2 | CRT0022-4-8-5 (T2 ) | -265 bp/-265 bp |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
