Name: Solyc10g085120
(Exon Intron)
Length: 3120
ATGAGGCAGTGGTATCTAACATCCAATTCCAAATGGCTTTTGTTGTTATACTTCCAGTACTCAATTATTGTTATGTCCATTGCAATATCAGGTCTCGAATCGACTGATCAATTAGCATTACAAGACTTGAAAAGCAGAATAACAGAGGATCCACTTCATGTCATGGCTTCTTGGAATGATCATTCTTCCCATTTCTGCAA
CTGGACTGGTGTAACATGTAGTCCAGGAAATGGAAGAGTTACGTTTCTTGATTTGAGTTCACGACAACTTGCTGGAACCATACCGTCTTCCATGGGTAAT
CTCAGTTTCCTTACAGGTATTGATCTTGGAAACAATAGTTTCCGCGGTGAAATTCCTCAAGCTATAGGCCGCCTGTTGCAGCTACAACATTTAAATGCTA
GTTACAATTATTTTAGTGGGAAAATTCCTACTAATTTAACTTACTGTAAGGAACTAAGAGTACTTGATCTACAATTTAATGAACTTGTTGGGAAAATTGT
GGATCAACTAAGTTCATTGTCGAAGCTGTATTTGTTTAAACTGAAAAGGAACTCACTCGGAGGAAACATCCCCCGTTGGTTAGGGAACTTTTCATCTTTA
GAGTTTTTTGACATTTCGGGTAATAGTCTACAAGGACCAATACCTGAGGAACTTGGGCGTCTAACGAAGTTGTTAGTGTTTCATGTGAACTCTAACGAAC
TATCTGGTACAATCCCTCCATCGATTCTCAATATTTCCTCCATATACTATTTCTCTGCTACACAAAATATACTGCATGGACAACTTCCAGCAGATGTAGG
CCTCACTCTTCCAAACCTTGAAGTATTTGCTGGTGCTGTCAACAGCTTTACCGGACCTATTCCTGTTTCACTAGCTAATGCTTCTAAGCTTCGCGTGATT
GATTTTTCCCAAAATAAACTTACTGGGGATGTGCCAACTAGTTTTGGAAAGTTGGAAACGTTGGTTAGGCTTAACTTTGAGGCGAATAGACTAGGTGGGA
GGGGAAGTTATGAGGGCTTGAAATTTCTTGATTCCCTAACTAATTGTACACATCTGATGGTACTGAGTTTCGCCACCAATAACTTTAGGGGAGAATTGCC
TTACTCAATCACTAATCTTTCAACTGTACTTGAGATTTTCTCTCTAGGTCAAAATAGGCTGCATGGTACTCTTCCAGCTGGAATAGACAACCTGATTAGT
TTGACGCTACTAGGAATGGACGGAAACTACCTCAATGGGAGTGTGCCTGAATCTATAGGTAAGCTTGAATATCTTGAACGTCTCTATTTGAACGGAAATG
CATTTTCTGGGAAGATACCATCCTCTATTGGTAACTTGTCATTGTTAAATACTCTAAATCTGGACGAAAATAGACTAGAAGGAAGCATACCTCCGGAGTT
GGGGAAGTGTAAGTTCTTGTCAACGTTAAACCTTACCAGAAATAATCTCGTTGGCTCCATACCAAAAGAGGTTGCAGGATTATCTTCCCTTTCAATTTCT
TTATCATTGGGAAGTAATTCTTTGACTGGATCCCTGCCAAAAGAACTTGATCAGTTGATAAATCTCGAGGAATTGGATTTATCACAGAACAAGATATCTG
GTGAGATTCCAAGCACCCTCAGCAATTGCCTCCACTTAGAGCGTGTATATATTAGTAATAATCTCTTACAAGGAACCATTCCTCAATCCTTTATGAGTTT
AAAAGGATTGGAAGAGATAGATTTTTCGCGGAACAACTTGTCAGGGGAAATACCTGAGTTTCTTGGGGAACTTTCATATCTCAGAAAACTGGACCTTTCA
TTCAATGAATTTGAAGGTGAGGTGCCAAATGAAGGGGTCTTTTCCAATACAAGTGCAATTTCAATCAAAGGAAACAGGAAACTTTGTGGAGGTGTCTCCG
ACTTGCATTTGCCTGAATGCTCCAAAGCTCCGAAGCACCTTAATTCAAGAGTGTGGATAGCTGTTAGTGTTCCTGTTGCACTCTTAGCTTTGGTGCTATG
TTGTTGTGGTGGTTATTACAGGATCAGAAACTCAAGAAAGGCACACCCTTGGATCGAACAGCTAGCCCAGATACCAAGAACTACATATCGAGAAATACTT
AGAGCAACGGATGGTTTCTCTGAGGCTAACTTGGTAGGTACAGGAAGTTTTGGTTCAGTATATAAAGCGCATTTCCATGGTGAGGAAACAATAATGGCAG
TGAAAGTGTTGAACTTACAACAAAGAGGGGCCTTGAAGAGCTTCCTGGATGAATGTAGAGCTTTAAGAAATATAAGGCATCGTAATCTTCTCAAAATCAA
GACTGCTTGTTCGAGCATTGATCACCAAGGTAATGACTTCAAATGCCTAGTTTTTGAGTTCATGAGTAACGGAAACCTACACGATTGGTTGCATCCAGAA
AATGATGACCAGCAACACCAAACAAACAAGCTGACCATTATCCAAAGACTAAACATTGCCATAGATGTTGCTTCTGCACTCGACTATCTCCACAACAACT
GTCAAACACCGATAGTTCATTGTGATCTAAAACCAAGCAACATACTCCTCGATGAAGATATGTCTGCCCATGTTGGTGACTTTGGATTAGCAACATTTCT
CCTCGACACATCAAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAACAGGTACAACGATGTCATTAGTTTAC
ATTTTAATATTCAGTCATATATCATGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCAACCATCCACACTTGGAGATGTTTACA
GCTTTGGAATAGTGTTGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACAGCTTT
GCCTGAACATGTTATGGAGATTGTAGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCAAGCAAGAAGAGTGGAAGAATGTCTA
CTCTCGGTTTTGGAAATCGGGCTTACATGTTCTGCATCATCATCTAGAGATAGAGCACCAATAGACACTATTTTAAGCAAACTTCAAGCAATCCGCGAAT
CCTTCCTTACAAGGAGATGA
The mutation and phenotype for selected feature: Solyc10g085120
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19 (T0 ) | -1 bp/-3 bp/-4 bp/-5 bp | |
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19-1 (T1 ) | -1 bp/-4 bp/-5 bp | Morphology : Oval cotyledon (M82 sp+: elongated) |
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19-1-14 (T2 ) | -1 bp (76.4%); -5 bp (21.6%) | Morphology : Oval cotyledons; low seed set |
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19-1-14-2 (T3 ) |
-1 bp/-1 bp
Is tDNA present: No
Mutation sequence: GGTGACTTTGGATTAGCAACATTTCTCCTCGACACATCAAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGG TTCTATAGGTTACATCCCAACAGGTACAACGATGTCATTAGTTTACATTTTAATATTCAGTCATATATCATGCTGCAGAA AATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGCCAACCATCCAACTTGGAGATGTTTACAGCTTTGGAATAGTGT TGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGCAATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACA GCTTTGCCTGAACATGTTATGGAGATTGTAGACCCCTTATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCA AGCAAGAAGAGTGGAAGAATGTCTACTCTCGGTTTTGGAAATCGGG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Morphology : Oval cotyledons; delayed flowering; parthenocarpic fruits |
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19-1-14-3 (T3 ) |
azygous
Is tDNA present: No
Mutation sequence: CCAAGCAACATACTCCTCGATGAAGATATGTCTGCCCATGTTGGTGACTTTGGATTAGCAACATTTCTCCTCGACACATC AAGTAATTCTTGGAGCCATCAGATTTCTGCAGCACTAAAGGGTTCTATAGGTTACATCCCAACAGGTACAACGATGTCAT TAGTTTACATTTTAATATTCAGTCATATATCATGCTGCAGAAAATTGATTTAGTACCTTGCAGAGTACGGATCAGGCGGC CAACCATCCACACTTGGAGATGTTTACAGCTTTGGAATAGTGTTGTTAGAGTTGTTCATCTGTAAAAGACCAACTGATGC AATTTTCAACGAGAGTCTAAACATTCACAAGTATGTTTCAACAGCTTTGCCTGAACATGTTATGGAGATTGTAGACCCCT TATTGCTCTTGGCAGAGGAAGAGCAAAACATCAATCAAGATCAAGCAAGAAGAGTGGAAGAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0137 | M82 sp+ | CTG0137-48 | CGR0137-48 | CRT0137-19-1-14-6 (T3 ) | -1 bp (43.7%); -5 bp (50.1%) | Morphology : Oval cotyledons |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
