Name: Solyc11g069430
(Exon Intron)
Length: 2671
TCACGCATTGCTTCTGAATGAACCAAGAGCTTTAATTGCTCCGGCTCGGAGAATGTACTGGTGGTAGACAGCGGCGATGAAAGCTCCGATAAATGGCCCGACCCAGAAAATCCAGTGTTCGTCCCACGCCTTATCACCGTTGAAAATCACAGCTGCCCCGAAACTCCTCGCCGGGTTGATTCCGGTACCGGTGATCGGAA
TAGTGGCAAGGTGAACCATGAATACGGCAAATCCAATGGGGAGTGGGGCCAAGACCTTTATTTAAGACGTAAAAAAAGGTAAAATAATTTTTAAAAAAAT
TATTCATAACCAATTTTTATTAAAAACTATATAAAAAAAACACGATAGATAACATAACAAAATAAATATTAAACAACAACAAAATTTTAAAGTACTTCTT
TTTTATCTTTAATGAGCTAATATATTATTATATACATATATTCACTTATTTTAAACATATGTTTTAATTTAAAAACTTCATATTAATTTAAATTTATTAT
ATAATAATTTAGTGATATGATAGGTGTATATCTTTAATATCATTACAAACTAAGCAAAATCACTTCATTATATTGTATTTTCTAAAGTGTTTTAGTTAGG
TCACCTCCCACTTTAATAAATCAAGAAAAATAATTAGATTAATAATGGGTAGGCCACTTACTAATTAACACCACACAAAAAAATGCTTCAAATATTAAAG
ATAAGATTTGTTTTTAGCCACATTATTGATTTTTAGTGGGGAAATTAATTAAAGTAAACCCCCCACCCACCCAAACCCTAATCCTACCTACCCCCTAAAC
AAGCTAAACTAGACTAGAGTATACAATATAATAATACTTTATTTTGTGTCATATCATTATGTAACGTTGACAACAGATTCAGTATTTAAAATATGTGAAG
TCTGAATTCGATTTTACTTACAGGGACATGAGAGTCTCTGGCGCTTCGTTTAGGGTCGGTGGCGGAGAAGACAACATAGACCAATACAAAAGTACCAATG
ATTTCAGCAGCCAAACCAACACCCTTGGTGTGTCCTCCTGCCATGACATTAACACCACCACCATATCTATTGTAGTATGCACTTTGAAAAGCCTTAACAA
AACCTACACCACAAATTGCACCCAAACATTGTGCTACCATGTACAATATAGCCCTAATCAATGACACCTTCCTTGCCAAAAATAGCCCAAATGTCACAGC
AGGGTTGATGTGTCCACCTACAATCCATCACATATATTATCAGTATATTTTAATCTGTTATAATAATCGTAAAGAATTCGAACAAAGCAGAAACAAGATT
GATTTTATATGACATGTTATGTGTAAAAGCTTTAAGAATGGTCTAAATTTTCCCTTTTATTTGAAACTAATATGTCAAAGCTTCATTTACTCATATTCGT
AAGCCTGGAAGAGCAAAATCGAGCCACTTTGCTAGCGACAAAGGTAAGAAACGAGACAATTTTTTAACGAAGGGTTAAACTGTATCATTTTTTCGTAGTT
TATAGTCATTTTAACCCTTTCACCCTACTTGTTTTAACATCAAAAATATTAAATTCAATTGAACCCTAAAAATAAATTACTACTTGATAAGGACGAATAT
ATTTTAACTTGTTATAATAATCGTAAAGAATTTGAACAAAGCAAAAACAAGACTGGTTTTTTTGTTTAAAAACTTAGTCCATGGTCTAAAATTCCCTTTT
AATTGTTGTTTTTAGTATATATAGTTGGATACAAGTATTTAGCTCTACGATGAATTTATATCTATTAATTATCGTAATTTCAGTTGATTTTTTTACACAT
ATATTTATATTTTACATTCAAAATTCCTGAATTCATCTAAATACCTCATAGCTCCATTTATCTCGTGAAAAAAAAAGAGAAAAATCGAGCCAATTTACTA
GCGACAAAGGTAAGAAATGAAACATTTTTTTTTAACAAATATCAAAAATTGTTTACTTTTTCTTAATATATGGCCATTTTTAACCCTTCACTCTACTTAT
TTTTATTTCGAAAAAATACTAAATTCAAGCGAACGATAAAACATAAACTAAACATATCCACTTCTTTCTAATATCACTTAAACAAAGGACACTAACAAGA
CAACCTATATAGTTATGAATTCGACCGAACTTAATACCTTTTACGTATAAACTCAAAAACGAATTCCTACTTATTTTTATTTCAAAATTACTAAATTCAA
CCGAACGATAAAACATAAACTAATCATATCCACTTCTTTCTAATATCACTCAACAAAGGACACTAACAAGGCAACCTATATAGTTATGAATTCGACAGAA
CTTAATATCTTTTACGTATAAATTCAAAAACGAGTAAATTCGAAAAAAAAAAACTCACCAGAAATACCAGCAGTACAGTAAACAAGAACAAAGATCATGC
CACCAAAAGCCCAAGCAATACCAAGTAAACCAACACCACCGCAAATATCGCCTCCGGCTTTAACATCAGCTTGATGTTTGTAACCAATTACTGTAAGAAT
AGTTATATATAGAAATAACAGAGTAGCAATAAACTCCGCTATTGCTGCTCTGTATAGTGACCATTTGGTCAATTCCTCGAAATCGATCAGAGGAGCAGGC
GGTGGATCAGTGTAATCCTTTGCGGAGTACTCCACCGCCTGCTCGTGAACCGCTTCGACTTCTTTCGTCAT
The mutation and phenotype for selected feature: Solyc11g069430
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-2 (T0 ) | +1 bp (33.6%); WT (62.7%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-2-1 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: CACTGATCCACCGCCTGCTCCTCTGATCGATTTCGAGGAATTGACCAAATGGTCACTATACAGAGCAGCAATAGCGGAGT TCATTGCTACTCTGTTATTTCTATATATAACTATTCTTACAGTAATTGGTTACAAACATCAAGCTGATGTTAAAGCCGGA GGCGATATTTGCCGGTGGTGTTGGTTTACTTGGTATTGCTTGGGCTTTTGGTGGCATGATCTTTGTTCTTGTTTACTGTA CTGCTGGTATTTCTGGTGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-2-3 (T1 ) |
+1 bp /+1 bp
Is tDNA present: No
Mutation sequence: CACTGATCCACCGCCTGCTCCTCTGATCGATTTCGAGGAATTGACCAAATGGTCACTATACAGAGCAGCAATAGCGGAGT TCATTGCTACTCTGTTATTTCTATATATAACTATTCTTACAGTAATTGGTTACAAACATCAAGCTGATGTTAAAGCCGGA GGCGATATTTGCCGGTGGTGTTGGTTTACTTGGTATTGCTTGGGCTTTTGGTGGCATGATCTTTGTTCTTGTTTACTGTA CTGCTGGTATTTCTGGTGA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-3 (T0 ) | +1 bp (46.2%); WT (48.4%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-3-4 (T1 ) | WT (47.1%); +1 bp (47.5%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-4 (T0 ) | +1 bp (34.6%); WT (62%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-4-3 (T1 ) |
WT
Is tDNA present: No
Mutation sequence: GCAAAGGATTACACTGATCCACCGCCTGCTCCTCTGATCGATTTCGAGGAATTGACCAAATGGTCACTATACAGAGCAGC AATAGCGGAGTTTATTGCTACTCTGTTATTTCTATATATAACTATTCTTACAGTAATTGGTTACAAACATCAAGCTGATG TTAAAGCCGGAGGCGATATTTGCGGTGGTGTTGGTTTACTTGGTATTGCTTGGGCTTTTGGTGGCATGATCTTTGTTCTT GTTTACTGTACTGCTGGTATTTCTGGTGAGTTTTTTTTTTTCGAATTTACTCGTTTTTGAATTTATACGTAAAAGATATT AAGTTCTGTCGAATTCATAACTATATAGGTTGCCTTGTTAGTGTCCTTTGTTGAGTGATATTAGAAAGAAGTGGATATGA TTAGTTTATGTTTTATCGTTCGGTTGAATTTAGTAATTTTGAAAT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-4-9 (T1 ) | +1 bp (47.6%); WT (47%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-7 (T0 ) | WT (48.2%); +1 bp (46.9%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-7-2 (T1 ) | WT 48.4%; +1 bp (46.2%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-7-2-3 (T2 ) | WT | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-7-2-7 (T2 ) | +1 bp /+1 bp | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-8-5 (T1 ) | WT 26.4%; +1bp (70.8%) | |
| CRT0059 | RG-prf3 | CTG0059-1 | CGR0059-1 | CRT0059-8-5-2 (T2 ) | +1 bp/+1 bp | |
| CRT0138 | RG-PtoR | CTG0138-29 | CGR0138-29 | CRT0138-13 (T0 ) | -12 bp (41.7%) / -8 bp (42.1%) / -7 bp (2.3%) | |
| CRT0138 | RG-PtoR | CTG0138-29 | CGR0138-29 | CRT0138-22 (T0 ) | -1 bp (46.5%) / -2 bp (49.3%) | |
| CRT0138 | RG-PtoR | CTG0138-29 | CGR0138-29 | CRT0138-40 (T0 ) | -1 bp/-1 bp |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
