Name: Solyc11g072910
(Exon Intron)
Length: 2505
TTATCTACCCTCAGACTTGACCAATTGTGAGAAAACTCTACTCATGGAAACACCAGAGAGATCATCCAAATTTGGTGTTCCGTTATGAGCAGCAGAAACACCGGGATCAACTTGACTGAAATCATCGACTTGTGGAGAGAGCTCGCCAATAAGGATGGTGCTGTTTTCTTCAGGATCATCTTGAATGACAGCTTCTTGAA
GTTGAAGTGCATACTCCAGATTCCACAACACATCGCCCATTGATGGCCTGTCTACACCAAAATCAGCTAAGCATTTCTCTGCTGTTTCTCCAAACTTCCT
GAGAGAATCTGGTCTTATTTTGCCCTCAAGATTGGAATCTATTATTTGTTCCAGTTGTCCCATCTTCTGCCACTTCATTGCCCATTCAGCTAAGTTGACC
ATCTCTCTCGGAAGAGATGGATCTATGACAGGCCTAGCACAAAGAACTTCAAATAAAACAACACCGAAAGAATAAACATCAGATTTTTCTGTCAGTTGTT
GCCTTCTAAAATATTCAGGATCTAGGTAGCCAAAACTTCCTTTCACGGCAGTGCTAACATGAGTTTGATCAAGCTCAGGCCCTGTCTTGGATAGTCCAAA
ATCAGCAACTTTTGCCATAAAACTCTCATCAAGCAGTATGTTTGCGGACTTGACATCACGATGTATTACTGCCTTAGCATAGCCAGTATGAAGGTAGTGC
AGACCTCTGGCCGACCCGATGCATATCTCCAGCCTCTGCTTCCAGCTCATACTGGGTAGATCTGACCCATACAGATGGCTCTTGAGGGTCCCATTCTCCA
TATACTCATAAACTAGAATCATTTCATTTTTTTCATCACAGTATCCCATCAATGAAACCAGATGCCGATGGCGGAACTGAGAAAGCATCTCAATTTCCGT
TCGGAACTCTGCAATACCTTGTTGGGACTTGGGATTACCCCTTTTCACAGCCACCTTTGTACCATCATACAAAACACCCTTGTATACCTTCCCAAAGCCA
CCTATTCCAATGACCAAACTCTCATCGAAGTTGCTCGTTGCTTCCAGCAAAGCTGCAAAAGGAACGCGATAACTCATGTTTGAAGCAGCACTTATGGTTG
TACCGTTTGAATACTTGCTTCCCATGGTGTGTGAAGTGCCACCATTCATAGATACAGGAATCCATATTTTTGACTGGGCTAACTTTTCTTGTTTTCTTCT
TCTATGCATGCAAAATAAGATGCCAACCATCACCAACAAAACTGGGATACCTATACACACACCCACGATGACTCCGACATTCTTCTTTGCCTTTGAACTC
GGATTAGTAGGAACAGAACCTACCTGACTAAGGCTGCCCTGAGAATTATTCAACTTCAGAAGTTCCAGTCCATTTAGGAAAGCATCAGGATAAGCACTCC
TTCGAGATGGGCCCACACTAACGTTAAGCCTATTACTTTTAGCGGTTGGAGTAACATAGTCATTGTAATAAGCTGTGGCCAAAATTCCCTGAACTTTCTG
ACCAGGATCAAAATCAGAAGCCACGTTCCAGGAGTTCACATAAATGTTAAACAGCAGCTGATTGGCAGCTATACTCACTATGTCACAGAAGTGTAAGCGA
ATGAAATATTGGAACCCAGGATCCACATTAAATGTCCAGGTCACGTTAAAATTGGCATTAGAATCATCACCCGCACCAGCCACGTTCATCTCACTACAAG
TACCATAAACTGACGGTGGTGCAAAATCTGGTGTTGCTCCATCTGCTGGATATTTTACGGATCCAATCTTACTAACCGACTTAGCTGAACTCGGCTGGAT
CAAGAAACTTTGATCAGTAACCCAAGTTCTCCAAAGTGTATCATTATCAAACGACACCAATGATCCACCCATGTTTACCCTAGCAACCGTTTCGAGAGCC
TGTGCATACATACCACGGAATGTCCCAGCTGGACTAATGGTGAAGGCATCATCAGTTATAAGGACATCAGGTACAGAGACAATTTCCATAGCATTTATAT
AAGCAAATGAATTGCTCGAAGGAGAGAACGTTACTACAAGGTCATTCGAGGTAACATTTACTGAAAATTCCTTGACAGAAGCATTTTTTGGAGAGAAATT
GCCAAGAAGAACATTATTTTGAGTGGAAACAGAGAACATTGCAGTGCTCATATCATAAATTTGGTAAACAAACGGGTAAAAATAAAGGCGAATCCAATGC
CTCCCCTTCAGGCTAATTGGGAATTTATAGCTGGATTGTTGAGTAAAAATTCTAGCAGTTTGATAAAGGGGTGAATCATTAGCTTTTGTAATTGAATTTG
AAGGGGTATCAGCCAATATATCTTTTGAAGTTGACAAAAACTTTGAAGCTGATTTATCAGCCATAAAGACCCTATTGCCAACATTAGTATCTTTAGATGA
TCCACAATCTACTAAGAAATTATCAGCAGGTTCAAAACCAGAAACACACAGAACTCCCAACACCAATATTATCAAACTCTTCCCAAACAATCCAAAACCT
TCCAT
The mutation and phenotype for selected feature: Solyc11g072910
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0003 | RG-prf3 | CTG0003-1 | CGR0003-1 | CRT0003-1 (T0 ) | WT (45%); -4 bp (43%) | |
| CRT0003 | RG-prf3 | CTG0003-1 | CGR0003-1 | CRT0003-1-49 (T1 ) | WT(-45%); -4 bp (-35%) | |
| CRT0003 | RG-prf3 | CTG0003-1 | CGR0003-1 | CRT0003-1-49-121 (T2 ) |
-4 bp/-4 bp
Is tDNA present: Yes
Mutation sequence: TTGGGAAGAGTTTGATAATATTGGTGTTGGGAGTTCTGTGTGTTTCTGGTTTTGAACCTGCTGATAATTTCTTAGTTGTG GATCATCTAAAGATACTAATGTTGGCAATAGGGTCTTTATGGCTGATAAATCAGCTTCAAAGTTTTTGTCAACTTCAAAA GATATATTGGCTGATACCCCTTCAAATTCAATTACAAAAGCTAATGATTCACCCCTTTATCAAACTGCTAGAATTTTTAC TCAACAATCCAGCTATAAATTCCCAATTAGCCTGAAGGGGAGGCATTGGATTCGCCTTTATTTTTACCCGTTTGTTTACC AAATTTATGATATGAGCACTGCAATGTTCTCTGTTTCCACTCAAAATAATGTTCTTCTTGGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0004 | TA537 | CTG0003-1 | CGR0003-1 | CRT0004-5 (T0 ) | heterozygous | |
| CRT0004 | TA537 | CTG0003-1 | CGR0003-1 | CRT0004-5-16 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: TTGGGAAGAGTTTGATAATATTGGTGTTGGGAGTTCTGTGTGTTTCTGGTTTTGAACCTGCTGATAATTTCTTAGTATTG TGGATCATCTAAAGATACTAATGTTGGCAATAGGGTCTTTATGGCTGATAAATCAGCTTCAAAGTTTTTGTCAACTTCAA AAGATATATTGGCTGATACCCCTTCAAATTCAATTACAAAAGCTAATGATTCACCCCTTTATCAAACTGCTAGAATTTTT ACTCAACAATCCAGCTATAAATTCCCAATTAGCCTGAAGGGGAGGCATTGGATTCGCCTTTATTTTTACCCGTTTGTTTA CCAAATTTATGATATGAGCACTGCAATGTTCTCTGTTTCCACTCAAAATAATGTTCTTCTTGGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
| CRT0004 | TA537 | CTG0003-1 | CGR0003-1 | CRT0004-5-6 (T1 ) | -3 bp/-2 bp/-1 bp/WT | |
| CRT0004 | TA537 | CTG0003-1 | CGR0003-1 | CRT0004-6 (T0 ) | heterozygous | |
| CRT0004 | TA537 | CTG0003-1 | CGR0003-1 | CRT0004-6-38 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: TTGGGAAGAGTTTGATAATATTGGTGTTGGGAGTTCTGTGTGTTTCTGGTTTTGAACCTGCTGATAATTTCTTAGTATTG TGGATCATCTAAAGATACTAATGTTGGCAATAGGGTCTTTATGGCTGATAAATCAGCTTCAAAGTTTTTGTCAACTTCAA AAGATATATTGGCTGATACCCCTTCAAATTCAATTACAAAAGCTAATGATTCACCCCTTTATCAAACTGCTAGAATTTTT ACTCAACAATCCAGCTATAAATTCCCAATTAGCCTGAAGGGGAGGCATTGGATTCGCCTTTATTTTTACCCGTTTGTTTA CCAAATTTATGATATGAGCACTGCAATGTTCTCTGTTTCCACTCAAAATAATGTTCTTCTTGGCA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
