Name: Solyc12g009020
(Exon Intron)
Length: 3887
TTATAGCTCAGTAAGTGTTGCCAATGGAGGTCCTGCGGAAGTGAAGTAAGATCCAAGATCGATATCCTGGTCATCGTACATGGTGATGAAAGGGTGACTCTGTATTACATGAATCCCAAATAGCCACTACATTAGTAATTGATCTATAAGCAACTTCGAGAAAGGCAAAAAAAAGTGATTATCACTACCATGAGATCATT
TGCCGACAGTCTGTCCTTCTGGTGCTTCTGGACACTGAAACAAGTTATTGTGTAGTGAGCTTCCGAACTAGAAAACACATCTACCAGACACTCATAGGAC
AATTAGATTAGAAGTTTCAGATGGTACATAACTATAGCGCTTACCATGCAGATATGAATGAGCAGAATTGTGGAGAAAATTGGTCAGGAGGTGCACAAGG
TTCTGGTTGGTCAACTATGGTTTCCATAAGTTCATAGACATTGACCCATCCTTCATCTCCCTCGGGTGGTTTATATGGGAAATGACCTGTTGCACACTCG
AGCAAGACTAAACCCAAGCTCCAAATGTCGCTTTTGTAATCATAGGCACCTCCTGAAATTCTCTCTGGCTATAGCAAACAACAGAACATCAGCAGCAACT
TGGAGGTTTTTAGCATAGGCAGATTGTAATGCTCCCTCTATTTCAATTTGTTTGTCTAGTTTTGACTTGGCACGGAAATTAAGTAAAGAAAACTTTTGAA
CCTCATGGTCTTAAATTAAAGATATGTCAAATGTACCAAAATGTCGTTTAATTTTGTGGTCTTAGATATGTTGCATGAAAAGTTGGAATTAAGAAATTGC
CAAAAAAGGAAAGAGGCATTCTTTTTTCAACGGACTAAACAAACAAATTGAAACGGAGGAAGTGATTAAGTTCCAGAAAAATATGCTAGGAAAATATTTC
TCTGTGATATTTATGAGTGAAAATTACCTAGCAGCGCAGTTCTATATTCGCTTCTACAAAAATCAGGTCACAGATACTCACAGACATATAGTTGTATGTG
CCGACAAAGGTATTGGCCAGTCCAGATGTGCTTGCTAGTACTGCACTCACACCAAAGTCTGTGATTTTGACATCACCTCTGTGATTGATTAGCAAATTCG
AAGGTTTCAAATCCCTGTGAATAATATGCTTCTCATGATGAAGATACCACAAGCCTTTGAGAACCTAATTGGTGAAGGAATACGGTTGAAACATTAGTAC
TTGAACAAATTCTTCTCCTGTGGTATTTAAATTGTTCACTGGCTACCTGTTTGCAGATAACAGCAAGAAATCGTTCAGGTATTGTTTTGACCTTTTTCAG
AAAATCTGCTAAGGAACCACCATCCATATACTCCAAAATCAAGGATATAGCACCATTGTCGAAGAACGACTGATAGCATATGACAACATATGGACACTGG
GATGACTGATTAATTCTCAGTTCTTGAGCAATATGTTTGCGCATAGACTCATCAATATTCATCTGAATAACCTGAATGATATAAAACTGTTCGTCAGTAA
AGGAACAAATTAAAGCATACGTCAACATAAGCTAACAGATTGTCCAATAAACAAACCCCTCCATCAGCATTGCGAACAGGTTTGGAGCCAGGTAGGACCA
AGGGGATTCATCTTAACCCCCTTGGGCGGAAAATTACACTACACAGGTTAAAATTATTTTTTATGTAGATATAGTAGATCGTGAACCCCATTTGGCTTCT
TTGTGTATTATCATTCATATTTAGAACCCCCTATTGAAAATTATGGCTTCCACTGAATTTGCAAAGAACAAGTCAATATGATCTTTGTGTAATAAAATGG
TGAGACTTGAAAGCACCCTAGTATTGGGTAAAATAGCAATCTCAATATTCAGAAACTGATTGAGATAGTCATACTGTCAACTCTTCAGGCTTTGGTGAGG
GACTCTCCAAAAATGTCGATGCACCCGTGTCGGATCCTCCAAAAAATGCACTACTTATGAAAGATTCGACACGCACCCGATGACATTTTTGAAGAGTCCA
CGCAACATAGTTAACATATTGCTACCAAAAATAGTACAAGTTATAAGACAAGAAATGGAACAATAGGGAGGAAACATCTCTATGAAAGAATTCGCGTCCC
AATCGTCAATTTGTCTTCTATCATTTTTTAAGAAGGAATATCGCACCAAATTAATGAAATATATCAAACGGATATGTAAACAGTCTGCTGCTACTCAGGG
GAATAGCAAACAACCAGGATAAGCAGAATAGAGATGGAAGCTGAAATATACCTTGAGAGCGAAAAATTGCCCTGTCCATTTATGCTGAACCAGCCGCACT
ATACCACCATTTCCCTTTCCAATAACTTTTACTGCTTCAAAATCAGCTAAGCATAACTGGTTGTCTGATGGCTGTATAACTGAAGGCTGAAAAAAAATTG
CAAATGCCAGAATAAATACGCCATTATAGTTGCAACGGATTGCGAAAATGGATATAAAGGAAGATGAAGAACACAAAATGTCCAGGTATGGACTAAAACG
ACGACTGCACCTCAACTCCTAGCAAGTTGGCATCAGGTAAGTGAATCCCCACTGCTCTTATCGCTCCATTAAAGTCCGTCTTAGTCCGATAAATTTAAAA
CAGTAGTTCCTCTAGAACCAGAGGTTCTCTACATTTCCGTATATAAATTTCTAACAAGACTAAGAGGCTCCTACCAAATTCTGGACCTAAAGTAAACGTA
CTAATATAACAAAGAGTCATTCCCGCTAATTAGTATCAGTTATATAAAAATATGTCTATCTCTAGATTCAAATACGTAAATTGTTATTTGGACCCTTCAT
ATAAGATGAGTCCCTAATCACAGCTTTAATATCCTGATTCAGAAAATCAGCTTTTAGATCTAGATCTTCAATTTTTCTTCCATTTCAAATTCTTTATAAG
CTGGCTGGGAGTAAGCATTCAAAAATTTCAATTCTTTATGCATTTAGATTTTCTAGCCATATCCTGTGTAATTTTACAAGTGGAGTATGGGGAAGGTTGA
GTGTACCCCGACCTTACCCTTACCTAATGGATGTAGAGAACTTGTTTTCGAAAAACCCTCGGCTCAATGCAGCAAATCAAAGTATTTACGGAAAAGGGAA
TAGACCAGTGTTGAAAACATGTCAATTACTAAGGAAAGCAGTACAAAGCATTCAAAGAAAAGAATGTGGTAACTGAAACACAATGAACACCAGATATCAA
AGGCAAGAAAATACATGTATGAGACTAATACTACAACAGACACAGTGTTCCCCGACACCCACCAATCTTAAACCCGTCGAAAAATGAGTCAAAGCTCAAT
TACCTACTTACCTTCTACCCTAATCCGCAACATCCATACCCTCCTATCTCAGGTCAGCTAAACATTCCAATTCAAACTTCAAACTTCCAAAGGCAAATTA
GAGAATAGTCTACTCTTAATCCCATGTAAACTAAGCAAAGGATCATCCTACTACCCAATCCAAGCAAGACTACAGAACTATAAAAGCAATTCAAACAAAT
CAAACCAACATTTCCTTTAATCAAATACAACATAAGTAATTCAAAAGAGAAAGTGTACAGCTGCAACTTCACTCTGCGAAACAATTCGAACTCCATCTCT
ATTCACCAGAAGATCTCCATCCTTAAATGTTCCTGATTCAGTCCTATACAACAAAATACCAAAAACATCTCATCAAAATACATAACCATAGCTAGAGAAA
CACAGAAATAAGAGATACAAGAAGTGGATCCAACAGTTCAAGTCAGTTGACACAAAGTACAGATGAATTTAGCATAAATGTGACATAAGAGAGAGGGATA
AAGTAAGTACAGGAATTTGGAGAGAGCAACTTCATCAGGAGGAGGAAGAGAGAGTTTAAGATTAGGTGCAAAAGATCCTTTCTTCAT
The mutation and phenotype for selected feature: Solyc12g009020
Project Name:Using CRISPR/Cas9 to understand and improve the plant immune system (NSF-IOS 1546625)
Diseases of crop plants have major economic and environmental impacts because they decrease yields, reduce marketability of produce and require extensive pesticide applications. The overarching goal o...... Lab: Martin Lab ; Contact: Gregory Martin: gbm7@cornell.edu| Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| CRT0094 | RG-PtoR | CTG0094-1 | CGR0094-1 | CRT0094-1 (T0 ) | -7 bp (8.1%); -2 bp (33.3%); WT (48.5%) | |
| CRT0094 | RG-PtoR | CTG0094-1 | CGR0094-1 | CRT0094-1-2 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: TCACATTTATGCTAAANTCATCTGTACTTTGTGTCAACTGACTTGAACTGTTGGATCCACTTCTTGTATCTCTTATTTCT GTGTTTCTCTAGCTATGGTTATGTATTTTGATGAGATGTTTTTGGTATTTTGTTGTATAGGACTGAATCAGGAACTTAAG GATGGAGATCTTCTGGTGAATAGAGATGGAGTTCGAATTGTTTCGCAGAGTGAAGTTGCAGCTGTACACTTTCTCTTTTG AATTACTTATGTTGTATTTGATTAAAGGAAATGTTGGTTTGATTTGTTTGAATTGCTTTTATAGTTCTGTAGTCTTGCTT GGATTGGGTAGTAGGATGATCCTTTGCTTAGTTTACATGGGATTAAGAGTAGACTATTCTCTAATTTGCCTTTGGAAGTT TGAAGTTTGAATTGGAATGTTTAGCTGACCTGAGATAGGAGGGTATGGATGTTGCGGATTAGGGTAGAAGGTAAGTAGGT AATTGAGCTTTGACTCATTTTTCGACGGGTTTAAGATTGGTGGGTGTCGGGGAACACTGTGTCTGTTGTAGTATTAGNCT CATACATGT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Immunity response: More susceptible to DC3000dd |
| CRT0094 | RG-PtoR | CTG0094-1 | CGR0094-1 | CRT0094-1-3 (T1 ) | "-2 bp (47.1%), -4 bp (46%)" | Immunity response: More susceptible to DC3000dd |
| CRT0094 | RG-PtoR | CTG0094-1 | CGR0094-1 | CRT0094-2 (T0 ) | -1 (50.4%); -9 (41.6%) | |
| CRT0094 | RG-PtoR | CTG0094-1 | CGR0094-1 | CRT0094-2-2 (T1 ) | -1 bp | Immunity response: More susceptible to DC3000dd |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
