| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-1 (T0 ) |
heterozygous
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-1-5 (T1 ) |
-2 bp/-2 bp
Is tDNA present: No
Mutation sequence: GCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCGGCACACAATTCAGCAGGTTA
GTTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGA
TATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAAT
TTGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGG
GGAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAG
TAATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGA
ACATTTATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAA
AGATTGCTTTGGTGTTTTACCTATCATTTTAGACTCTTTC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2920 GAAAGAGTCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGA 2969
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 GAAAGAGTCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGA 50
Wildtype 2970 TCATTTATCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCA 3019
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 TCATTTATCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCA 100
Wildtype 3020 AATACAAGCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTT 3069
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 AATACAAGCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTT 150
Wildtype 3070 AGGAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTA 3119
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 AGGAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTA 200
Wildtype 3120 CTACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTA 3169
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 CTACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTA 250
Wildtype 3170 TATTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAA 3219
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 TATTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAA 300
Wildtype 3220 TCACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTC 3269
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TCACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTC 350
Wildtype 3270 CTGAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAA 3319
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 CTGAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAA 400
Wildtype 3320 CTATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCT 3369
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 CTATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCT 450
Wildtype 3370 ACAGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGAT 3419
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 ACAGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGAT 500
Wildtype 3420 TCTCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGC 3469
||||||||||||||||||||||||||||||||||||||||| |||||||
MutationRev 501 TCTCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCC--GAATGGC 548
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3470 CCATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCA 3519
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 549 CCATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCA 598
Wildtype 3520 GC 3521
||
MutationRev 599 GC 600
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-2 (T0 ) |
heterozygous
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-2-27 (T1 ) |
-4 bp/-4 bp
Is tDNA present: Yes
Mutation sequence: TTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCCACACAATTCAGCAGGTTAGTTTTGT
CCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGCT
AACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATTT
CAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATGG
ATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTANTAATTGT
TGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3072 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACT 3121
||||||||||||||||||||||||||||||||||||||||||||||||.|
MutationRev 1 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTANT 50
Wildtype 3122 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 3171
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 100
Wildtype 3172 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 3221
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 150
Wildtype 3222 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 3271
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 200
Wildtype 3272 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 3321
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 250
Wildtype 3322 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 3371
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 300
Wildtype 3372 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 3421
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 350
Wildtype 3422 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCC 3471
||||||||||||||||||||||||||||||||||||| |||||||||
MutationRev 351 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTG----GAATGGCCC 396
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3472 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 3516
|||||||||||||||||||||||||||||||||||||||||||||
MutationRev 397 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 441
|
Immunity response: More susceptible to DC3000dd; decrease in ROS response to csp22
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-2-27-1 (T2 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: TTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCCACACAATTCAGCAGGTTAGTTTTGT
CCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGCT
AACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATTT
CAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATGG
ATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTANTAATTGT
TGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3072 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACT 3121
||||||||||||||||||||||||||||||||||||||||||||||||.|
MutationRev 1 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTANT 50
Wildtype 3122 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 3171
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 100
Wildtype 3172 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 3221
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 150
Wildtype 3222 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 3271
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 200
Wildtype 3272 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 3321
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 250
Wildtype 3322 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 3371
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 300
Wildtype 3372 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 3421
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 350
Wildtype 3422 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCC 3471
||||||||||||||||||||||||||||||||||||| |||||||||
MutationRev 351 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTG----GAATGGCCC 396
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3472 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 3516
|||||||||||||||||||||||||||||||||||||||||||||
MutationRev 397 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 441
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-2-27-2 (T2 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: TTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCCACACAATTCAGCAGGTTAGTTTTGT
CCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGCT
AACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATTT
CAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATGG
ATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTANTAATTGT
TGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3072 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACT 3121
||||||||||||||||||||||||||||||||||||||||||||||||.|
MutationRev 1 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTANT 50
Wildtype 3122 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 3171
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 100
Wildtype 3172 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 3221
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 150
Wildtype 3222 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 3271
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 200
Wildtype 3272 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 3321
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 250
Wildtype 3322 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 3371
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 300
Wildtype 3372 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 3421
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 350
Wildtype 3422 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCC 3471
||||||||||||||||||||||||||||||||||||| |||||||||
MutationRev 351 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTG----GAATGGCCC 396
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3472 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 3516
|||||||||||||||||||||||||||||||||||||||||||||
MutationRev 397 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 441
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-2-27-3 (T2 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: TTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCCACACAATTCAGCAGGTTAGTTTTGT
CCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGCT
AACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATTT
CAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATGG
ATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTANTAATTGT
TGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3072 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACT 3121
||||||||||||||||||||||||||||||||||||||||||||||||.|
MutationRev 1 GAATCCACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTANT 50
Wildtype 3122 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 3171
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ACTGTGCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATA 100
Wildtype 3172 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 3221
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TTCATTCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATC 150
Wildtype 3222 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 3271
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 ACAATAGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCT 200
Wildtype 3272 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 3321
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 GAAATCAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACT 250
Wildtype 3322 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 3371
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 ATTACTGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTAC 300
Wildtype 3372 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 3421
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 AGTTGGACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTC 350
Wildtype 3422 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCC 3471
||||||||||||||||||||||||||||||||||||| |||||||||
MutationRev 351 TCGATATAAGGACAAAACTAACCTGCTGAATTGTGTG----GAATGGCCC 396
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3472 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 3516
|||||||||||||||||||||||||||||||||||||||||||||
MutationRev 397 ATTATCAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAA 441
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4 (T0 ) |
heterozygous
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-10 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: ATGAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCATGGCACACAATTCAGC
AGGTTAGTTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGAC
AATTGATATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAG
TGGAATTTGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTG
TTGCGGGGAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCA
CAGTAGTAATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAA
GCTCGAACATTTATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAA
GTAAAAAGATTGCTTTGGTGTTTTACCTATCATTTTAGACTCTTTCTTCATCTAATGTCATGCTTTTGTTCATGGAACTT
TTGTTTAGTCCATAATTTTTTATTTCAATCCTACCTAACTGGAGAACTTTTTTATAGGCAACAATGGAGGTCAGCGTGCC
ACAGAAAGTGCACAAAGAGAGACACAGACTGTGAATATCCAGCCCATTGCTGTTCCTTCCATCGCTGTTGATGAGTTAAA
GGATATCACTGATAACTTTGGTTCGAAAGCTTTGATAGGTGAGGGATCATATGGAAGGGTATACCATGGTGTCCTGAAAA
GTGGACGTGCTGCAGCCATTAAAAAATTAGACTCAAGTAAGCAGCCTGATCGAGAATTTTTAGCTCAGGTTAGCTACACC
CTATTATCTTTCATTTGACTATGTTATATTGCACTCAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 2576
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 50
Wildtype 2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 2626
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 100
Wildtype 2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 2676
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 150
Wildtype 2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 2726
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 200
Wildtype 2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 2776
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 250
Wildtype 2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 2826
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 300
Wildtype 2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 2876
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 350
Wildtype 2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 2926
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 400
Wildtype 2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 2976
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 450
Wildtype 2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 3026
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 500
Wildtype 3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 3076
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 550
Wildtype 3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 3126
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 600
Wildtype 3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 3176
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 650
Wildtype 3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 3226
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 700
Wildtype 3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 3276
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 750
Wildtype 3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 3326
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 800
Wildtype 3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 3376
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 850
Wildtype 3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 3426
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 900
Wildtype 3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 3476
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 950
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 3525
|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 999
|
Immunity response: "No significant change in resistance to DC3000dd, ROS not tested"
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-13 (T1 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-4 (T1 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
Immunity response: More susceptible to DC3000dd; decrease in ROS response to csp22
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-4-1 (T2 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-4-2 (T2 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-4-3 (T2 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-4-4 (T2 ) |
-7 bp/-7 bp
Is tDNA present: No
Mutation sequence: CTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACAATTCAGCAGGTTAGTTTTG
TCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGATATAGC
TAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATTTGATT
TCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGGGAATG
GATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGTAATTG
TTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAACATTT
ATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAAGTAAAAAGATTG
CTTTGGTGTTTTACCTATCATTTT
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2930 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 2979
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 AAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTATCA 50
Wildtype 2980 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 3029
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 ATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAAGCA 100
Wildtype 3030 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 3079
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 TAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCAC 150
Wildtype 3080 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 3129
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCC 200
Wildtype 3130 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 3179
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCC 250
Wildtype 3180 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 3229
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGA 300
Wildtype 3230 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 3279
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 TACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAA 350
Wildtype 3280 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 3329
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGC 400
Wildtype 3330 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 3379
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGAC 450
Wildtype 3380 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 3429
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATA 500
Wildtype 3430 AGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAG 3479
||||||||||||||||||||||||| ||||||||||||||||||
MutationRev 501 AGGACAAAACTAACCTGCTGAATTG-------TGAATGGCCCATTATCAG 543
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3480 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 3520
|||||||||||||||||||||||||||||||||||||||||
MutationRev 544 TAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAG 584
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-8 (T1 ) |
azygous
Is tDNA present: No
Mutation sequence: ATGAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCATGGCACACAATTCAGC
AGGTTAGTTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGAC
AATTGATATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAG
TGGAATTTGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTG
TTGCGGGGAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCA
CAGTAGTAATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAA
GCTCGAACATTTATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAA
GTAAAAAGATTGCTTTGGTGTTTTACCTATCATTTTAGACTCTTTCTTCATCTAATGTCATGCTTTTGTTCATGGAACTT
TTGTTTAGTCCATAATTTTTTATTTCAATCCTACCTAACTGGAGAACTTTTTTATAGGCAACAATGGAGGTCAGCGTGCC
ACAGAAAGTGCACAAAGAGAGACACAGACTGTGAATATCCAGCCCATTGCTGTTCCTTCCATCGCTGTTGATGAGTTAAA
GGATATCACTGATAACTTTGGTTCGAAAGCTTTGATAGGTGAGGGATCATATGGAAGGGTATACCATGGTGTCCTGAAAA
GTGGACGTGCTGCAGCCATTAAAAAATTAGACTCAAGTAAGCAGCCTGATCGAGAATTTTTAGCTCAGGTTAGCTACACC
CTATTATCTTTCATTTGACTATGTTATATTGCACTCAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 2576
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 50
Wildtype 2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 2626
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 100
Wildtype 2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 2676
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 150
Wildtype 2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 2726
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 200
Wildtype 2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 2776
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 250
Wildtype 2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 2826
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 300
Wildtype 2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 2876
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 350
Wildtype 2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 2926
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 400
Wildtype 2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 2976
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 450
Wildtype 2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 3026
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 500
Wildtype 3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 3076
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 550
Wildtype 3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 3126
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 600
Wildtype 3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 3176
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 650
Wildtype 3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 3226
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 700
Wildtype 3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 3276
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 750
Wildtype 3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 3326
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 800
Wildtype 3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 3376
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 850
Wildtype 3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 3426
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 900
Wildtype 3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 3476
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 950
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 3525
|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 999
|
Immunity response: "No significant change in resistance to DC3000dd, ROS not tested"
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-8-1 (T2 ) |
azygous
Is tDNA present: No
Mutation sequence: ATGAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCATGGCACACAATTCAGC
AGGTTAGTTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGAC
AATTGATATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAG
TGGAATTTGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTG
TTGCGGGGAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCA
CAGTAGTAATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAA
GCTCGAACATTTATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAA
GTAAAAAGATTGCTTTGGTGTTTTACCTATCATTTTAGACTCTTTCTTCATCTAATGTCATGCTTTTGTTCATGGAACTT
TTGTTTAGTCCATAATTTTTTATTTCAATCCTACCTAACTGGAGAACTTTTTTATAGGCAACAATGGAGGTCAGCGTGCC
ACAGAAAGTGCACAAAGAGAGACACAGACTGTGAATATCCAGCCCATTGCTGTTCCTTCCATCGCTGTTGATGAGTTAAA
GGATATCACTGATAACTTTGGTTCGAAAGCTTTGATAGGTGAGGGATCATATGGAAGGGTATACCATGGTGTCCTGAAAA
GTGGACGTGCTGCAGCCATTAAAAAATTAGACTCAAGTAAGCAGCCTGATCGAGAATTTTTAGCTCAGGTTAGCTACACC
CTATTATCTTTCATTTGACTATGTTATATTGCACTCAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 2576
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 50
Wildtype 2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 2626
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 100
Wildtype 2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 2676
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 150
Wildtype 2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 2726
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 200
Wildtype 2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 2776
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 250
Wildtype 2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 2826
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 300
Wildtype 2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 2876
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 350
Wildtype 2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 2926
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 400
Wildtype 2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 2976
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 450
Wildtype 2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 3026
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 500
Wildtype 3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 3076
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 550
Wildtype 3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 3126
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 600
Wildtype 3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 3176
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 650
Wildtype 3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 3226
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 700
Wildtype 3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 3276
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 750
Wildtype 3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 3326
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 800
Wildtype 3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 3376
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 850
Wildtype 3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 3426
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 900
Wildtype 3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 3476
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 950
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 3525
|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 999
|
|
| CRT0005 |
Moneymaker |
CTG0005-1 |
CGR0005-1 |
CRT0005-4-8-2 (T2 ) |
azygous
Is tDNA present: No
Mutation sequence: ATGAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCATGGCACACAATTCAGC
AGGTTAGTTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGAC
AATTGATATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAG
TGGAATTTGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTG
TTGCGGGGAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCA
CAGTAGTAATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAA
GCTCGAACATTTATTATGCTTGTATTTGATGTTTTCGTGGTCAGTTCCGGTTGAAGCCTTGTAAATTGATAAATGATCAA
GTAAAAAGATTGCTTTGGTGTTTTACCTATCATTTTAGACTCTTTCTTCATCTAATGTCATGCTTTTGTTCATGGAACTT
TTGTTTAGTCCATAATTTTTTATTTCAATCCTACCTAACTGGAGAACTTTTTTATAGGCAACAATGGAGGTCAGCGTGCC
ACAGAAAGTGCACAAAGAGAGACACAGACTGTGAATATCCAGCCCATTGCTGTTCCTTCCATCGCTGTTGATGAGTTAAA
GGATATCACTGATAACTTTGGTTCGAAAGCTTTGATAGGTGAGGGATCATATGGAAGGGTATACCATGGTGTCCTGAAAA
GTGGACGTGCTGCAGCCATTAAAAAATTAGACTCAAGTAAGCAGCCTGATCGAGAATTTTTAGCTCAGGTTAGCTACACC
CTATTATCTTTCATTTGACTATGTTATATTGCACTCAAC
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 2527 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 2576
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 GTTGAGTGCAATATAACATAGTCAAATGAAAGATAATAGGGTGTAGCTAA 50
Wildtype 2577 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 2626
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTGAGCTAAAAATTCTCGATCAGGCTGCTTACTTGAGTCTAATTTTTTA 100
Wildtype 2627 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 2676
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ATGGCTGCAGCACGTCCACTTTTCAGGACACCATGGTATACCCTTCCATA 150
Wildtype 2677 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 2726
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 TGATCCCTCACCTATCAAAGCTTTCGAACCAAAGTTATCAGTGATATCCT 200
Wildtype 2727 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 2776
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 TTAACTCATCAACAGCGATGGAAGGAACAGCAATGGGCTGGATATTCACA 250
Wildtype 2777 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 2826
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 GTCTGTGTCTCTCTTTGTGCACTTTCTGTGGCACGCTGACCTCCATTGTT 300
Wildtype 2827 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 2876
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTATAAAAAAGTTCTCCAGTTAGGTAGGATTGAAATAAAAAATTATGG 350
Wildtype 2877 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 2926
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 ACTAAACAAAAGTTCCATGAACAAAAGCATGACATTAGATGAAGAAAGAG 400
Wildtype 2927 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 2976
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 401 TCTAAAATGATAGGTAAAACACCAAAGCAATCTTTTTACTTGATCATTTA 450
Wildtype 2977 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 3026
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 451 TCAATTTACAAGGCTTCAACCGGAACTGACCACGAAAACATCAAATACAA 500
Wildtype 3027 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 3076
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 501 GCATAATAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATC 550
Wildtype 3077 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 3126
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 551 CACTCCCCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGT 600
Wildtype 3127 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 3176
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 601 GCCTCAACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCAT 650
Wildtype 3177 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 3226
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 651 TCCGCTCTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAAT 700
Wildtype 3227 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 3276
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 701 AGATACCCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAAT 750
Wildtype 3277 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 3326
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 751 CAAATTCCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTAC 800
Wildtype 3327 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 3376
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 801 TGCTATGCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTG 850
Wildtype 3377 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 3426
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 851 GACTCATTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGAT 900
Wildtype 3427 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 3476
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 901 ATAAGGACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTAT 950
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3477 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 3525
|||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 951 CAGTAGCTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTCAT 999
|
|
| CRT0006 |
RG-prf3 |
CTG0005-1 |
CGR0005-1 |
CRT0006-15 (T0 ) |
heterozygous
|
|
| CRT0006 |
RG-prf3 |
CTG0005-1 |
CGR0005-1 |
CRT0006-15-10 (T1 ) |
-5 bp/-5 bp
Is tDNA present: No
Mutation sequence: GAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACACAATTCAGCAGGTTAG
TTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGAT
ATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATT
TGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGG
GAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGT
AATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAA
CATTTA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3033 TAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCACTCC 3082
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCACTCC 50
Wildtype 3083 CCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCCTCA 3132
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCCTCA 100
Wildtype 3133 ACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCCGCT 3182
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCCGCT 150
Wildtype 3183 CTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGATAC 3232
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 CTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGATAC 200
Wildtype 3233 CCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAAATT 3282
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 CCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAAATT 250
Wildtype 3283 CCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGCTAT 3332
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 CCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGCTAT 300
Wildtype 3333 GCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGACTCA 3382
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGACTCA 350
Wildtype 3383 TTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATAAGG 3432
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 TTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATAAGG 400
Wildtype 3433 ACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAGTAG 3482
|||||||||||||||||||||||| |||||||||||||||||||||
MutationRev 401 ACAAAACTAACCTGCTGAATTGTG-----TGAATGGCCCATTATCAGTAG 445
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3483 CTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTC 3523
|||||||||||||||||||||||||||||||||||||||||
MutationRev 446 CTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTC 486
|
|
| CRT0006 |
RG-prf3 |
CTG0005-1 |
CGR0005-1 |
CRT0006-15-7 (T1 ) |
-5 bp/-5 bp
Is tDNA present: No
Mutation sequence: GAGCTGCTTCAGTTGTTGTGATGATGATGATATGCACAGAGCTACTGATAATGGGCCATTCACACAATTCAGCAGGTTAG
TTTTGTCCTTATATCGAGAATCTCTTCCGATTGTTTTCTTAACGTTTTCTGGGGAATGAGTCCAACTGTAGACAATTGAT
ATAGCTAACTAGTTTGTCGTTGAGGCATAGCAGTAATAGTTGTTAGTTTTGGGTAAGGGAAAGGTTATGGTAGTGGAATT
TGATTTCAGGACTTCTACACTTATGGTGAATACTTGAGCATTCTGGGTATCTATTGTGATTCTTTTTTTCGTGTTGCGGG
GAATGGATCCAAATAGAGCGGAATGAATATAGACGATTGATACAGCTAAACAAACTAATTTATGGTTGAGGCACAGTAGT
AATTGTTGTCTAGTTTTGGGTATGGAAAAGGATAGGGGAGTGGATTCCTAACTGGGGTATCAGTAAATTGGAAGCTCGAA
CATTTA
Mutation alignment:
CRISPR-generated mutation natural variation gRNA exon
Wildtype 3033 TAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCACTCC 3082
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 1 TAAATGTTCGAGCTTCCAATTTACTGATACCCCAGTTAGGAATCCACTCC 50
Wildtype 3083 CCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCCTCA 3132
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 51 CCTATCCTTTTCCATACCCAAAACTAGACAACAATTACTACTGTGCCTCA 100
Wildtype 3133 ACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCCGCT 3182
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 101 ACCATAAATTAGTTTGTTTAGCTGTATCAATCGTCTATATTCATTCCGCT 150
Wildtype 3183 CTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGATAC 3232
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 151 CTATTTGGATCCATTCCCCGCAACACGAAAAAAAGAATCACAATAGATAC 200
Wildtype 3233 CCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAAATT 3282
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 201 CCAGAATGCTCAAGTATTCACCATAAGTGTAGAAGTCCTGAAATCAAATT 250
Wildtype 3283 CCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGCTAT 3332
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 251 CCACTACCATAACCTTTCCCTTACCCAAAACTAACAACTATTACTGCTAT 300
Wildtype 3333 GCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGACTCA 3382
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 301 GCCTCAACGACAAACTAGTTAGCTATATCAATTGTCTACAGTTGGACTCA 350
Wildtype 3383 TTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATAAGG 3432
||||||||||||||||||||||||||||||||||||||||||||||||||
MutationRev 351 TTCCCCAGAAAACGTTAAGAAAACAATCGGAAGAGATTCTCGATATAAGG 400
Wildtype 3433 ACAAAACTAACCTGCTGAATTGTGTGCCATGAATGGCCCATTATCAGTAG 3482
|||||||||||||||||||||||| |||||||||||||||||||||
MutationRev 401 ACAAAACTAACCTGCTGAATTGTG-----TGAATGGCCCATTATCAGTAG 445
gRNA GCTGAATTGTGTGCCATGAA
Wildtype 3483 CTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTC 3523
|||||||||||||||||||||||||||||||||||||||||
MutationRev 446 CTCTGTGCATATCATCATCATCACAACAACTGAAGCAGCTC 486
|
|
