Transformation | Genetic background | Construct | gRNA | Plant | Mutation | Phenotype |
---|---|---|---|---|---|---|
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-3 (T0 ) | -11 bp (27%); WT (69.8%) | |
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-4 (T0 ) | -1 bp (10.8%); +1 bp (26.3%); -6 bp (35.3%) | |
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-4-1 (T1 ) |
+1 bp/-2 bp
Is tDNA present: No
Mutation sequence: TCTTTGAACTTGACGATTTCTGTCTTTTTCGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGA CAATTTGGTTCAATGCAGCCAAATGCAGATCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAG CTACTATATCTTTAAAGACTTAAAATCTGTAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGG ACTGGAGAGAGTGGCCCCATTTCACGAGGCTCAGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCA TCCTACTGCAGTGTTTAATAAATTTTTTTTCACGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCAT TCTGCGAGTTTACATTTGAGCAGCTCAGGATAGCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAA AAAGCTCCAAATGTTGTTTATAAAGGAAAGCTAGAGAACCAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGC CTGATTCCCGGCAATTTTTAGTAAGAGCTTGTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCAT ATCCATCTACTAAAATGATATAATTTCCTTTTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGA AATTTAAAATAAAATAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-4-2 (T1 ) |
+1 bp/-2 bp
Is tDNA present: Yes
Mutation sequence: TCTTTGAACTTGACGATTTCTGTCTTTTTCGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGA CAATTTGGTTCAATGCAGCCAAATGCAGATCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAG CTACTATATCTTTAAAGACTTAAAATCTGTAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGG ACTGGAGAGAGTGGCCCCATTTCACGAGGCTCAGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCA TCCTACTGCAGTGTTTAATAAATTTTTTTTCACGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCAT TCTGCGAGTTTACATTTGAGCAGCTCAGGATAGCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAA AAAGCTCCAAATGTTGTTTATAAAGGAAAGCTAGAGAACCAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGC CTGATTCCCGGCAATTTTTAGTAAGAGCTTGTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCAT ATCCATCTACTAAAATGATATAATTTCCTTTTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGA AATTTAAAATAAAATAG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-5 (T0 ) | -2 bp (49.1%); -6 bp (46.5%) | |
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-5-10 (T1 ) | -2 bp/-6 bp | |
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-5-2 (T1 ) |
-2 bp/-2 bp
Is tDNA present: Yes
Mutation sequence: CGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGACAATTTGGTTCAATGCAGCCAAATGCAGA TCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAGCTACTATATCTTTAAAGACTTAAAATCTG TAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGGACTGGAGAGAGTGGCCCCATACGAGGCTC AGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCATCCTACTGCAGTGTTTAATAAATTTTTTTTCA CGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCATTCTGCGAGTTTACATTTGAGCAGCTCAGGATA GCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAAAAAGCTCCAAATGTTGTTTATAAAGGAAAGCT AGAGAACCAGAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGCCTGATTCCCGGCAATTTTTAGTAAGAGCTT GTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCATATCCATCTACTAAAATGATATAATTTCCTT TTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGAAATTTAAAATAAAATAGTAATGAGAATAAT TG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-5-8 (T1 ) |
-2 bp/-2 bp
Is tDNA present: Yes
Mutation sequence: CGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGACAATTTGGTTCAATGCAGCCAAATGCAGA TCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAGCTACTATATCTTTAAAGACTTAAAATCTG TAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGGACTGGAGAGAGTGGCCCCATACGAGGCTC AGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCATCCTACTGCAGTGTTTAATAAATTTTTTTTCA CGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCATTCTGCGAGTTTACATTTGAGCAGCTCAGGATA GCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAAAAAGCTCCAAATGTTGTTTATAAAGGAAAGCT AGAGAACCAGAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGCCTGATTCCCGGCAATTTTTAGTAAGAGCTT GTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCATATCCATCTACTAAAATGATATAATTTCCTT TTTCTTTTCTTATATTAAATGTTGACGATGATATTCATGACAAGAATAGAAATTTAAAATAAAATAGTAATGAGAATAAT TG Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0012 | RG-PtoR | CTG0012-2 | CGR0012-2 | CRT0012-7 (T0 ) | -1 bp (49%); -2 bp (47%) | |
CRT0013 | Hawaii 7981 | CTG0012-2 | CGR0012-2 | CRT0013-1 (T0 ) | -2 bp (48.9%); -4 bp (47.4%) | |
CRT0013 | Hawaii 7981 | CTG0012-2 | CGR0012-2 | CRT0013-2 (T0 ) | Large deletion (~-140 bp); overlapping | |
CRT0013 | Hawaii 7981 | CTG0012-2 | CGR0012-2 | CRT0013-2-1 (T1 ) |
-4 bp/-4 bp
Is tDNA present: No
Mutation sequence: CGATTTCTGTCTTTTTCGGGTGTCTAACTAAGATAACCTTACAAGAAGCACAATGCACTTGAATTGACAATTTGGTTCAA TGCAGCCAAATGCAGATCTTGAAGCCGCATAAAGTGTAATTTGTCGGGTTGTGCTACAAACTCCAAGCTACTATATCTTT AAAGACTTAAAATCTGTAGGATAGTGTGATGGGCTGTGAAAGTTCTAAACTTGCCTCATGTTGCTGGACTGGAGAGAGTG GCCCCATTAGGCTCAGAATCCTGGTATGTATATCTTTTATTGAAATATTTTCTATATAACCATCCTACTGCAGTGTTTAA TAAATTTTTTTTCACGCTTCTCGTAGATGAAGAAAAAAATGAAGTTAGTGATTCACCTGCATTCTGCGAGTTTACATTTG AGCAGCTCAGGATAGCTACATCTGCATTTGCTGTAGAGAATATAGTTTCTGAACATGGCGAAAAAGCTCCAAATGTTGTT TATAAAGGAAAGCTAGAGAACCAGAGGCCGGTTGCTGTTAAACGCTTCAACAGATCTGCATGGCCTGATTCCCGGCAATT TTTAGTAAGAGCTTGTCTGTTAATTCCTTGGTATATTTCAACTCTATGGAAAAGACGTTCCCATATCCATCTACTAAAAT GATATAATTTCCTTTTTCT Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
|
CRT0013 | Hawaii 7981 | CTG0012-2 | CGR0012-2 | CRT0013-2-6 (T1 ) | -4 bp/-140 bp | |
CRT0013 | Hawaii 7981 | CTG0012-2 | CGR0012-2 | CRT0013-9 (T0 ) | -1 bp (71.2%); -4 bp (26.4%) |
This database is supported by NSF (IOS-1546625) and hosted by BTI.