Plant Information
Name: ncbp-1/2 #8
Seed stock:
Seed availability: No
Seed availability: No
Relationship:
Transformation: nCBP-1
transformation nCBP-1 to 60444
transformation nCBP-1 to 60444
| Plant ID | Transformation | Construct | gRNA | Target Feature | Mutation | Phenotype |
|---|---|---|---|---|---|---|
| ncbp-1/2 #8 (T0 ) | nCBP-1 | nCBP-1 | nCBP1-sgRNA | gene : Manes.09G140300 (nCBP-1) | -1 bp and -3 bp | Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
| ncbp-1/2 #8 (T0 ) | nCBP-1 | nCBP-1 | nCBP2-sgRNA | gene : Manes.08G145200 (nCBP-2) |
-1 bp
Is tDNA present:
Mutation sequence: ATGGAGATCACTGAGAAGAAGGATACAGAGAACAACACAAACAACAGCAATAATAATGCTCAAACGACATTGGATTCAGC ATCACTTGAGAACATAGACAAAGAAGCCGAAGAACGCCAAGCACGCGACCTCAAAGCTGGGTTGCATCCTCTCAGGTTCT CTCTTTCGCTCTTTTATGTGTCTATCTGTTGTTTAATTCTATATGATTAGATCTTCGGTCCCTGCTAGTTTTTGCAGTTT GATTATTTTGCTATGAATTAGAGGAATTATGAAGCCAATCAATGAATCATTTCCTATGATTTTTGTATTTTTTTTTTCCT GAAGTGGGTTATACTGGATTTATGCTACTAAGAGAAATTAAGGCTAAAGGCTAAGAAGTTGTTA Mutation alignment: CRISPR-generated mutation natural variation gRNA exon |
Delayed and attenuated cassava brown streak disease (CBSD) aerial symptoms, reduced severity and incidence of storage root necrosis, and reduced virus titre in storage root, when challenged with Cassava brown streal virus (CBSV). |
This database is supported by NSF (IOS-1546625) and hosted by BTI.
