Is tDNA present:

Mutation sequence:

TACCACAAGAGTTAACTGGAGGGGTTCCCTTCCCAGGGATATACTTTGTATGTTTTGTCGCCAGCATCGCCTGTCTGAAC
CTCTCTTTTCCATTATAAGTCATCCTCTCAAAATACCAACTGAATCATCAGAGTCGTGTTTTAAGGCTGCAGGCTCTGGA
ACTGATGTGATTGAATGTGTCAATGGGGCCTCTGTAAATGGATGTCCAAAGCCAGGGATGTTTAAATGTGAAATAAAGTT
GCTTTCTCGGTGTGCGGAAGTAATACTTTTGTGTTCCCCTGATGATTGTTACAAGAAGCAGAATGATGCTATTCAGAATG
CCTCATTGAAACTTCTATCATGGCTGAACAAGTATTTTAATAGTGTAACTGCTCCTTTTGAACAGCTCTA

Mutation alignment:

CRISPR-generated mutation  natural variation  gRNA  exon

Wildtype        3327 ATCAGAAGTGAGCAAGTTCGTGTTGAAATAAGCACACTGG----------   3366
                     |.||.|||      ||||                .|||||          
Mutation           2 ACCACAAG------AGTT----------------AACTGGAGGGGTTCCC     29

Wildtype        3367 --CCAACAGACATCTGCATCACAACCTCCTCAATGTATGAAATCACAGCA   3414
                       ||.|..||.||          ||.|      ||||||...|..|..||
Mutation          30 TTCCCAGGGATAT----------ACTT------TGTATGTTTTGTCGCCA     63

Wildtype        3415 CCAACCCCAATCTCAAACTCAAACT----CATTACAAGATTCAATAACTT   3460
                     .||.|.||..|||.||.|||  .||    |||||.|||            
Mutation          64 GCATCGCCTGTCTGAACCTC--TCTTTTCCATTATAAG------------     99

Wildtype        3461 CTTTCATATTTTCACCCTC-CACA--ACCAAAGATACTGAATAACTAATA   3507
                                |||.|||| ||.|  ||||     |||||||        
Mutation         100 -----------TCATCCTCTCAAAATACCA-----ACTGAAT--------    125

Wildtype        3508 CAAGGTAAAGATCCATTGCATGGACAGACACCAGAGTC-TGGACCTTCAA   3556
                                                 ||.||||||| ||         
Mutation         126 ----------------------------CATCAGAGTCGTG---------    138

Wildtype        3557 TCTTTAAGTTGCAAACGCCATTTACTAACATGTCTTGTAATGAATTTGCA   3606
                      .||||||        ||                            ||||
Mutation         139 -TTTTAAG--------GC----------------------------TGCA    151

Wildtype        3607 CACATTGGCTC---AAGTGAT-TTATTACACTGCAT-TG-CATAAAGATG   3650
                           |||||   ||.|||| |.||     ||.|| || ||     |||
Mutation         152 ------GGCTCTGGAACTGATGTGAT-----TGAATGTGTCA-----ATG    185

Wildtype        3651 GCGATTCTG--AATGGATTGGCCAATTAG-AATCTCTCTGAAAAGG----   3693
                     |.|..||||  |||||| ||.||||  || ||||||..||...|||    
Mutation         186 GGGCCTCTGTAAATGGA-TGTCCAA--AGCAATCTCGGTGTGCAGGCTCA    232

Wildtype        3694 ----TTTTTGGAAAAAACGTGGATGCTGAAGTTTCCTGCAGTCTCATAGA   3739
                         |.|||     |||.|||.|  .|.||||    |||..||||     
Mutation         233 GGGATGTTT-----AAATGTGAA--ATAAAGT----TGCTTTCTC-----    266

Wildtype        3740 GCTGTTCAAAAGGAGCAGTTACACTATTAAAATACT----TGTTCAGCCA   3785
                     |.|||.|    |||  |||           ||||||    |||||  ||.
Mutation         267 GGTGTGC----GGA--AGT-----------AATACTTTTGTGTTC--CCC    297

Wildtype        3786 TGAT----------AGAAGTTTC--AATGAGGC-ATTCTGAATAGCATCA   3822
                     ||||          |||||   |  |||||.|| ||||.|||| ||.|||
Mutation         298 TGATGATTGTTACAAGAAG---CAGAATGATGCTATTCAGAAT-GCCTCA    343

Wildtype        3823 TTCTG---CTTCTTGTAACAATCATCAGGG--GAACACAAAAGTATTACT   3867
                     |  ||   |||||          ||||.||  ||||    |||||||  |
Mutation         344 T--TGAAACTTCT----------ATCATGGCTGAAC----AAGTATT--T    375

Wildtype        3868 TCCGCACACCGAGAAA----GCAACTTTATTTCACATTTAAACATCCCT   3912
                     |             ||    |.||||   ..|| |.|||.||||.|.||
Mutation         376 T-------------AATAGTGTAACT---GCTC-CTTTTGAACAGCTCT    407